The effect of Froriepia subpinnata on the performance, carcass characteristics, blood parameters, immune system, microbial population, intestinal morphology, and breast meat fatty acid content of broiler chickens.

The present experiment was conducted to investigate the effects of F. subpinnata powder (FSP) on the performance, carcass characteristics, blood parameters, immune system, microbial population, intestinal morphology, and percentage of fatty acids in the breast meat of broiler chickens. A total of 300 day-old male chickens from the ROSS 308 strain were used in the form of a completely random design with four treatments of five replicates (15 birds each). The experimental treatments were, respectively, (1) control with base diet (without additives), (2) base diet + 1% FSP, (3) base diet + 2% FSP, and (4) base diet + 3% FSP. The results showed that the feed intake and weight gain increased in the treatments containing 2% and 3% FSP when compared to the control (P < 0.05). Cholesterol and ALT levels in the treatment containing 3% FSP were lower that the control (P < 0.05), while the concentration of glutathione peroxidase enzyme in the treatment containing 3% FSP significantly increased (P < 0.05). Thymus weight and antibodies produced against AIV in all three levels of FSP increased significantly compared to the control (P < 0.05). The population of lactobacilli and coliforms in the treatments containing FSP increased and decreased significantly compared to the control (P < 0.05). The length and width of the intestinal villi of the chickens that were fed with 3% of FSP had a significant increase compared to the control (P < 0.05). The percentage of saturated fatty acids in the breast decreased significantly with the consumption of all three levels of FSP (P < 0.001). In general, the results showed that the use of 3% FSP in the broiler diet increased the efficiency of growth performance and enzyme activity, while strengthening the immune system, favorably altering the intestinal microbial population, and reducing the fat in breast meat.

Effects of in ovo feeding of vitamin E or vitamin C on egg hatchability, performance, carcass traits and immunity in broiler chickens.

The effect of in ovo feeding of different levels of vitamins C and E on egg hatchability, immune response, growth and carcass traits of broiler chickens were investigated. A total of 672 fertilized eggs were assigned to one of eight experimental groups having three replicates with 28 eggs as follows: (1) negative control (not injected); (2) positive control (injected with 0.2 mL deionized water); (3) vitamin C at 1 mg; (4) vitamin C at 3 mg; (5) vitamin C at 6 mg; (6) vitamin E at 0.5 IU; (7) vitamin E at 0.75 IU; and (8) vitamin E at 1.0 IU. At the end of incubation, the number of chicks hatched, and their individual body weight were recorded. Among hatched birds, a total of 240 mixed chicks were randomly selected (30 subject per group equally shared in three pen floors). Chicks were vaccinated against Avian Influenza, Gumboro, Bronchitis, and Newcastle disease virus. Performance parameters were weekly evaluated until 42 days of age. At days 28 and 42, broiler serum and spleen and Bursa of Fabricius relative weight were assessed as well as on day 42 the carcass traits. From results, in ovo injection with 3 mg of vitamin C or 0.75 IU of vitamin E, increased significantly (p < .05) the embryos hatchability when compared to the negative control. However, body weight at hatch and growth performance parameters showed no differences among treatments. Similarly, in ovo concentrations of vitamins C or E showed no differences on carcass traits, immunity-related organs weight or immune response for anti-Newcastle disease hemagglutination-inhibition and total immunoglobulins against sheep red blood cells (SRBC) when compared to the control groups. Based on findings, it can be concluded that in ovo feeding vitamins E and C supported positively chicken embryos hatchability demonstrating the key-role as antioxidant agents; however, further studies are currently being evaluated.

The effects of peppermint (Mentha piperita L.) and chicory (Cichorium intybus L.) in comparison with a prebiotic on productive performance, blood constituents, immunity and intestinal microflora in broiler chickens.

A total of 320 one-day-old broiler chickens were used in a 42-day feeding trial to evaluate the effects of peppermint (Mentha piperita L.) and chicory (Cichorium intybus L.) in comparison with a prebiotic on-growth performance, blood constitutes, immunity and intestinal microflora. The dietary treatments were as follows: basal diet (control); control + prebiotic (Fermacto™); control + 0.1% peppermint; control + 0.1% chicory, respectively. A significant (p < 0.05) body weight gain and feed intake was found at 21 and 42 days of growth period in broilers fed diet supplemented with 0.1% chicory compared with other groups. Feeding of prebiotic or chicory led to higher (p < 0.05) feed intake. Chickens fed control diet had higher (p < 0.05) abdominal fat compared with the other groups. Serum blood constituents indicated that broilers fed prebiotic or supplemented with peppermint or chicory had reduced (p < 0.05) levels of cholesterol, triglycerides and low-density lipoprotein than control group. Immunity-related parameters showed that chicken fed chicory had lower (p < 0.05) heterophil-to-lymphocyte ratio compared with the other groups. Intestinal microflora revealed that chickens fed prebiotic or herbals had higher count of Lactobacillus and lower E. coli than control. Thus, it can be concluded that broiler dietary supplementation with prebiotic or chicory can improve performance supporting positively health status.

Birth weight and stillbirth genetic parameter estimation in a river buffalo simulated population.

Stillbirth (SB) is a threshold character that has been detected as the most important factor affecting the future reproductive life of an animal. In buffaloes, stillbirth accounts for approximately 42% of all reproductive disorders being considered as the main reproductive disorder in buffalo calves. The present study aimed to estimate the heritability values and to determine the genetic and phenotypic correlation between stillbirth rate and birth weight (BW) using a Bayesian approach via Gibbs sampling method in a river buffalo population. A stochastic simulation was used to create a population in which random mating was used for 15 discrete generations. A bivariate animal model including maternal effects was used. Maternal effects were significant (p < .05) for both BW and SB. The results showed that when the SB rate increased from 1% to 50%, the estimated direct heritability of SB increased. Yet, by increasing the SB rate from 50% to 99%, a decrease of the SB estimated direct heritability was observed. The genetic correlation between BW and SB was decreased by increasing the level of occurrence of SB in the population from 1% to 99%. The root mean square error (RMSE) of heritability was increased from 40% to 70% level of occurrence of SB. Based on findings, it can be concluded that to reduce stillbirth rate in the population, it is possible to use genetic improvement program and birth weight could be one of the main components of a selection goal.

The effect of vitamin C and aspirin supplementation on the performance of turkeys under normal conditions.

This study aimed to investigate the effect of vitamin C and aspirin on the growth performance of turkeys under normal conditions. For this purpose, 288 male turkeys, BUT6 strain, were used from 1-day-old to 12 weeks of age in nine treatments with four replications and eight birds per replication, as a 3 × 3 factorial experiment [three levels of vitamin C (V; 0, 250, and 500 mg/kg feed), and three levels of aspirin (A; 0, 75, and 150 mg/kg feed)] in a completely randomized design. Our results demonstrated that supplementing with vitamin C and aspirin had no effect on plasma total protein and albumin levels, but the data revealed significant effects on total cholesterol, triglycerides, very low-density lipoprotein (VLDL), and low-density lipoprotein (LDL) levels in the supplemental groups. Also, the interaction of vitamin C and aspirin significantly increased high-density lipoprotein (HDL) and decreased plasma malondialdehyde (MDA) and abdominal fat pad. The results of this study indicated the beneficial effects of vitamin C and aspirin supplements may be related to changes in plasma lipids and body fat under normal conditions.

Effect of dietary flaxseed meal supplemented with dried tomato and grape pomace on performance traits and antioxidant status of laying hens.

This study was carried out to determine the effect of dietary flaxseed meal (FSM) supplemented with dried tomato pomace (DTP) and dried grape pomace (DGP) on performance, egg quality, biochemical parameters traits and antioxidant status of laying hens. Birds (1825 ± 87 g of body weight) were divided into 12 dietary groups with six replicates per group (eight birds per replicate), under a completely randomized design with factorial arrangement 2 × 3 × 2 consisted of two levels of DTP (0 and 15%), three FSM levels (0, 4 and 8%) and two levels of DGP (0 and 5%). As a result of this study, there were no significant differences in egg production and weight as well in feed conversion ratio (FCR) among treatments (p > 0.05). Feeding of DGP reduced significantly feed intake and egg mass when compared to control group (p < 0.05). There was no effect (p > 0.05) of dietary treatment on shell thickness and strength, shape index, Haugh unit and egg specific gravity. Hens consuming 15% DTP and 5% DGP revealed a significantly higher yolk color compared to the other dietary treatments (p < 0.05). Moreover, there was no difference among dietary treatments in terms of serum low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) cholesterol, atherogenic index, triglycerides, total cholesterol levels (p > 0.05). Serum antioxidant parameters as glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA), total superoxide dismutase (TSOD) and total antioxidant capacity (T-AOC) were not influenced by treatments (p > 0.05). Based on findings, FSM and DTP supplements did not significantly impact most of hens' performance indicators and egg quality parameters, whereas significant improvements were observed by feeding of 15% DTP and 5% DGP on egg traits, in particular on egg-yolk color that plays a key-role in consumer's choice. However, the supplementation of FSM and DTP or DGP even in laying hen diet is still controversial and further research is needed.

Beneficial and adverse effects of medicinal plants as feed supplements in poultry nutrition: a review.

Medicinal plants exhibit colossal impact on poultry industries by improving its performance and productivity. However, some of these plants show adverse influence too by decreasing egg production percentage, egg mass, and microbiota counts. Green tea, nettle, pennyroyal, yarrow, and alfalfa in the form of seed, powder, and extract had vast potentiality to improve immunity, reduce the growth of pathogenic microbes, and improve the viable counts of lactic acid bacteria. Lavender, Alfalfa, and Nettle powder were able to improve egg yolk color. Furthermore, ginger reduced fat content in meat and increased color intensity. Flax seed increased alpha linolenic acid content in tissue, and increased n-3 fatty acid content in breast as well as thigh tissue. Physiological assessment showed that green tea, lavender, nettle, pennyroyal, and yarrow improved poultry immunity. Lavender and nettle improved internal organ traits. Interestingly, the use of flaxseed improved quail egg hatchability. Plants metabolites, particularly carvacrol and thymol showed its pivotal role as natural growth promoters by affecting growth performances, nutrient bioavailability, and immunity of broiler chickens. Additionally, in recent years, micro-encapsulation or nano-encapsulation of plant extracts and its metabolites improved growth performances of broiler chickens, thereby suggested wide utilization of this technique as a potential alternative to antibiotic growth promoters in future. This review sheds a light on beneficial as well as no adverse effects of some of the direct-fed important medicinal plants and its metabolites in poultry nutrition in order to suggest its key role in future poultry enterprise.

Effects of different levels of dietary black cumin (Nigella sativa L.) and fenugreek (Trigonella foenum-graecum L.) and their combination on productive traits, selected blood constituents, microbiota and immunity of broilers.

The effects of inclusion of powdered seeds of black cumin (B) (Nigella sativa L.) and fenugreek (F) (Trigonella foenum-graecum L.) on productive traits, selected blood constituents, microbiota and immunity of broilers were studied. A total of 648 day-old chicks were randomly assigned to nine treatments, with four pen replicates, each with 18 birds, including three levels of B seed powder (BSP; 0, 5 or 10 g/kg) and three levels of F seed powder (FSP; 0, 5 or 10 g/kg) in a 3 × 3 factorial arrangement. Neither powder affected feed intake. The FSP increased (p = 0.048) feed conversion ratio (FCR), but decreased daily BW gain (p = 0.02) between days 0 and 21, while BSP increased daily gain between days 22 and 42 and overall (both p = 0.005). Abdominal fat was decreased (p = 0.003) by BSP. Blood constituents were unaffected by either powder, but ileal Escherichia coli were decreased (p = 0.039) at day 42. The BSP increased a range of immunological titers, where BSP affected positively the measured variables. The interactions between BSP and FSP, specifically on broiler carcass cuts, suggested that where BSP is included at 10 g/kg, the inclusion of FSP at the same level may provide no additional benefit. Thus, while either powder could be included separately, the co-inclusion of both at 10 g/kg is not recommended.

Influence of dietary L-carnitine and lysine-methionine levels on reproductive performance and blood metabolic constituents of breeder ducks.

The present study aimed to evaluate the influence of dietary supplementation of different levels of L-carnitine and/or lysine-methionine (Lys-Met) on reproductive performance of breeder ducks. Three L-carnitine (0, 75 and 150 mg/kg) and three lysine-methionine (100%, 110% and 120% above the NRC (Nutrient requirements of poultry, 1994, National Academy Press) recommendations) levels were fed to 180 breeder ducks (144 females and 36 males) in a completely randomized design for 49 days. Laying performance and reproductive traits were evaluated; additionally, uric acid, total protein total, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assessed. The Lys-Met above 100% NRC (Nutrient requirements of poultry, 1994, National Academy Press) recommendations with or without L-carnitine improved feed utilization (p < .05). Furthermore, Lys-Met above 100% recommendations without L-carnitine improved egg fertility and hatchability. Fertility and hatchability improved in breeders fed on L-carnitine with 120% Lys-Met (p < .05). Serum glucose increased and total cholesterol reduced on 100% Ly-Met without L-carnitine or 110% Ly-Met with 150 mg L-carnitine (p < .05). Glucose was reduced, while total cholesterol increased on 75 mg L-carnitine and 100% Lys-Met (p < .05). Increasing Lys-Met without L-carnitine reduced serum protein (p < .05). Albumin and ALT increased on 75 mg L-carnitine-100% Lys-Met and reduced on 150 mg L-carnitine-120% Lys-Met (p < .05). There were no interaction effects on globulin, uric acid and AST (p > .05). Thus, based on findings, breeder ducks responded to dietary Lys-Met more efficiently than L-carnitine; however, more research is needed to evaluate also economic aspects related to L-carnitine dietary supplementation.

Excreta quality and digestive function of singly versus couple caged Sardinian partridges (Alectoris barbara barbara Bonnaterre, 1790) as non-invasive indicators of birds' coping ability to forced pairing.

The Sardinian partridge is a monogamous wild bird species of least concern according to IUCN list at present, though formerly accounted among threatened species for decades. The creation of couples is crucial in captivity, because forced pairing in cages can lead to poor welfare of birds, specially of females. This study investigated the impact of single versus couple caging of Sardinian partridges during non-laying period based on the collection and interpretation of indirect and non-invasive parameters (feed intake; space availability; excreta quality; and nutrient utilization). A total of 24 couples of breeding partridges were enrolled for two consequent phases of a same investigation (lasting 15 days each). During phase 1, all couples were fed ad libitum with a pelleted complete diet (DM, 905 g kg-1 of diet; CP, 160 g kg-1 ; and EE, 25 g kg-1 ). In phase 2, each male from 12 out of 24 couples was moved to an identical cage close to that where the female remained alone, to keep visual contact. The rest of couples continued to be kept like in phase 1. During phases 1 and 2, feed intake and excreta outputs were recorded daily. Pooled excreta of the last 3 days from couples and single birds were assessed for pH and DM, CP and ash content. Nutrient digestibility was calculated. No significant differences were noted between single versus couple caging regarding body weight (438 vs. 422 g respectively) or nutrient digestibility. However, singly housed males reduced daily feed intakes and females increased daily feed intake per g-1 BW (0.041 vs. 0.052 g, respectively, p < 0.001). Our results suggest that singly caged partridges from permanent couples can improve the access to feed and reduce competition during the non-mating season.

The effects of chelated micro-elements feeding in broiler breeder hens and their progeny: A review.

Micro-elements are essential for the optimal feeding of broiler hens. Since the chelated micro-elements negatively affect the content of micro- and macro-minerals, there has been a tendency to apply them as an alternative to common mineral forms of micro-elements in poultry diets. This paper reviews the effects of chelates of micro-elements (iron, copper, zinc, manganese, and selenium) on broiler breeder hens' internal and external egg quality and their egg products. The use of chelated micro-elements compared to the mineral forms does not have a significant effect on the performance of broiler breeder flocks, but they have significantly positive effects on the internal and external egg quality of broiler breeder flocks. The chelated micro-elements were also better than mineral resources of micro-elements in improving fertility (0.59%), hatchability (0.81%), and reducing mortality of progeny (2.25%). Chelated micro-elements have no negative impact on blood biochemistry or the immune system of broiler breeder hens and decrease cholesterol (0.84 mmol/l) and triglycerides (0.04 mmol/l) in most cases in comparison with the other mineral forms. Therefore, chelated forms of micro-elements instead of the inorganic mineral forms in diets of broiler breeder hens provide better protection for birds and the environment and also improve egg quality.

Effect of Cynara scolymus and its derivatives on broilers: an updated review.

Medicinal plants and their derivatives are human-friendly, nutritional supplements, and their use in the poultry industry is increasing today. Cynara scolymus is a very high-quality antioxidant medicine plant that has recently attracted the attention of poultry and nutrition researchers to supplement their rations and reduce feed costs. In the present study, the effect of powder and extract of C. scolymus on broilers has reviewed. This review shows that the impact of C. scolymus on production performance, carcass characteristics, liver enzymes activity, and meat characteristics of broiler chickens are not significant. However, the negative impact of this compound on these properties has not reported. Moreover, cholesterol levels reduced by the dietary C. scolymus supplementation. The impact of C. scolymus powder and extract on chickens' broiler immunity was not significant in the cited literature, but dietary C. scolymus powder increases Newcastle antibody titers and lowers blood pressure. Dietary C. scolymus under heat stress increased anti-sheep red blood cell titers. No substantial studies have conducted on the effect of C. scolymus on broilers. Therefore, it is not possible to determine a single dosage for the use of C. scolymus powder or extract in broiler diets to the improvement of the broiler characteristics. More researches are needed to determining the appropriate dosage to achieve maximum performance.

Effect of diets containing earthworm powder and vermihumus on egg production, hatchability, blood parameters and immunity of Japanese breeder quails.

The present study is aimed to evaluate the effects of different levels of earthworm (EW) powder and vermihumus (VH) on the growth performance, humoral immunity response, plasma constituents and reproductive performance of Japanese breeder quails. A total of 336 birds were assigned to seven treatments in a completely randomized design from day 30 to 90 of age. The dietary treatments were carried out, one without any addition of EW and VH, the second had a standard rate of VH at 0.8%, while a range of EW additions was supplied from 0%, 0.5%, 1%, 1.5%, 2% and 2.5%. The effects of treatments on body weight and mortality were not significant (p > .05). The highest feed intake was observed in birds fed the diets supplemented with 1% and 1.5% EW (p < .05). The highest titre of antibodies against influenza virus and sheep red blood cells belonged to the EW and VH treatments (p < .05). The antibody titre against Newcastle disease virus vaccine was not affected by the treatments. In general, the birds receiving EW and VH had the highest egg weight, egg production and egg mass weight (p < .05). Nonetheless, various levels of EW powder negatively affected hatchability and increased mortality and leg disorders (p < .05). The results showed that the levels of dietary 1% and 1.5% of EW powder gave the best growth and reproductive performance to birds respectively.

Effect of dietary inclusion of lecithin with choline on physiological stress of serum cholesterol fractions and enzymes, abdominal fat, growth performance, and mortality parameters of broiler chickens.

A total of 270 one-d-old Ross 308 broiler chicks were randomly allotted to 9 experimental diets (3 replicates of 10 birds each), including three types of supplemental lipotropic factors (control, 0.1% or 0.2% choline and 0.5% or 1% lecithin) in a 3 × 3 factorial arrangement. Supplementation of lecithin improved FCR values during 1-21 days of age. Although no differences were noticed for mortality index among different diets, the group supplemented with a combination of choline (0.1) and lecithin (0.5) showed the highest (P < 0.0001) production index. Choline (0.1% or 0.2%) significantly decreased serum total cholesterol by 11%, triglycerides by 21%, low-density lipoprotein (LDL) by 20%, and very low-density lipoprotein (VLDL) by 20%, while increased the glucose and high-density lipoprotein (HDL) values by 11% and 6%, respectively. On the other hand, lecithin significantly increased glucose, total cholesterol, triglycerides, HDL, LDL and VLDL by 4%, 9%, 7%, 24%, and 25%, respectively. Choline supplementation decreased the aspartate amino transferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (AP); however, the lecithin addition increased their respective proportions. This study concluded that the combinations of 0.1% choline and 0.5% lecithin is the best among all other treatments because of the highest production index and least mortality.

Extraction and purification of ß-glucanase from bovine rumen fungus Trichoderma reesei and its effect on performance, carcass characteristics, microbial flora, plasma biochemical parameters, and immunity in a local broiler hybrid Golpayegan-Ross.

The enzyme ß-glucanase was extracted from Trichoderma reesei in bovine rumen fluid samples collected from a slaughterhouse and its effect was investigated in broilers. Data collected was broiler performance, carcass characteristics, duodenum microbial flora, hematological, and immunological parameters. ß-glucanase activity was assayed through spectrometry and was approximately 0.434 IU per gram culture medium. In the current study, endoglucanase enzymes were extracted from Trichoderma reesei. A total of 160 local broilers (Golpayegan-Ross hybrid) were allocated to 4 treatments with 4 replicates per treatment. Over a 49-day experimental period, broilers were fed a basal diet (T1), basal diet plus 20% barley (T2), basal diet with 10 IU extracted ß-glucanase and 20% barley (T3), and basal diet with 10 IU commercial ß-glucanase and 20% barley (T4). The T3 treatment resulted in the greatest body weight gain at the end of experiment (P < 0.01). No significant differences were for feed conversion (FCR; P > 0.05). The highest cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), and LDL cholesterol ratio was observed in the T3 treatment. The highest concentrations of immunoglobulin G1 (IgG1), immunoglobulin G2 (IgG2), and immunoglobulin M1 (IgM1) were observed in the T4 treatment. The T3 treatment resulted in the best response for all measured carcass characteristics. The highest levels of aerobic bacteria, lactobacilli, anaerobic bacteria, and E. coli were associated with the T4, T3, T4, and T1 treatments, respectively. It is concluded that ß-glucanase supplementation can be used to overcome the anti-nutritive effects of water soluble barley non-starch polysaccharides (NSPs) and consequently enhance broiler performance without any adverse effects on humoral immunity parameters.

Effect of dietary sesame (Sesame indicum L) seed meal level supplemented with lysine and phytase on performance traits and antioxidant status of late-phase laying hens.

OBJECTIVE: This study was performed to investigate the effects of supplementing sesame seed meal (SSM) with phytase and lysine on performance, egg quality, blood biochemical and antioxidant status of laying hens. METHODS: A total of 960, 56-wk-old laying hens were divided into 12 dietary groups with eight replicates per group (10 birds per replicate). A completely randomized design with factorial arrangement 2×3×2 consisted of two levels of lysine supplement (0 and 10% over requirement), three SSM levels (0, 10 and 20%) with or without phytase (0 and 300 g/ton). The feeding trial lasted 10 weeks. RESULTS: From findings, birds fed diets with 10% SSM had higher feed intake than groups fed 0 and 20% SSM. The addition of phytase to experimental feeds, improved feed conversion ratio (FCR), increased egg weight and mass (P&lt;0.01). Egg quality criteria was not affected by supplementing phytase; however, supplementing 300 g/ton phytase to hens diet, led to a significant (P&lt;0. 05) increase in egg shell strength. Egg yolk cholesterol and serum low-density lipoprotein cholesterol (LDL-C), atherogenic index and total cholesterol were decreased (P&lt;0.01) by diet containing 20% SSM. The high-density lipoprotein cholesterol (HDL-C) was increased (P&lt;0.05) in serum of hens fed 20% SSM than the other groups. It was also observed that total antioxidant capacity (TAC) and total superoxide dismutase (TSOD) content of hens fed 20% SSM was significantly upper than control group (P&lt;0.05). CONCLUSION: As from results, dietary supplementation of SSM and phytase had no negative effects on laying hens performance or egg quality improving also the egg oxidative stability.

Dietary supplementation of Aspergillus oryzae meal and its effect on performance, carcass characteristics, blood variables, and immunity of broiler chickens.

This study investigated the effect of different levels and consumption periods of Aspergillus oryzae meal on performance, carcass characteristics, blood variables, and immunity of broiler chickens. A total of 270 (male and female) Ross 308 chicks were randomly assigned to 9 treatment groups. Two levels (2 g/kg diet and 4 g/kg diet as-fed) of Aspergillus oryzae meal (AO) and 4 consumption periods of AO (starter, grower, finisher, and entire period) in a 2 × 4 factorial arrangement were used. Compared with control, AO used during the entire rearing period increased weight gain, reduced relative weight of abdominal fat, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) serum levels, and increased antibody titers against influenza and Newcastle disease vaccination and sheep red blood cells injection. Few differences in the variables considered were found if AO was added to broiler diets only during specific consumption periods, and between the two supplementation levels of AO. In conclusion, the addition of AO to the broiler diet can have beneficial effects in terms of performance, carcass composition, and health, but these positive effects were mainly reached adding AO for the entire rearing period.

The effect of three tropical medicinal plants and superzist probiotic on growth performance, carcass characteristics, blood constitutes, immune response, and gut microflora of broiler.

The pivotal aim of the present context was to determine the effect of probiotic (superzist) and medicinal plants (R. coriaria, H. persicum, and M. piperita) powder on growth performance, carcass characteristics, blood parameters, immunity response, and microflora counts of broilers over a 42-day feeding period. One hundred and fifty one-day-old chickens were randomly allocated to the following five treatments: (1) Control diets; (2) control diets + 0.03% w/v superzist; (3) control diets + 0.5% w/v R. coriaria; (4) control diets + 0.5% w/v H. persicum; and (5) control diets + 0.5% w/v M. piperita. No significant differences among treatments on broiler performance during 29th to 35th days of age, whereas a gradual reduction (P < 0.05) in the feed efficiency and economic index values between 36th to 42nd days of age, across the treatments versus control diet. Supplementations were increased (P < 0.05), the colon weight, colon length, and right cecum weight versus control. However, the superzist and phytogenic supplementation slightly increased the jejunum length, ileum length, and left cecum length. The supplementation showed reduced heterophils, increased lymphocytes and monocytes percentage, and decreased heterophils/lymphocytes ratio. Additions of superzist and medicinal plants reduced (P < 0.05) the total counts of E. coli and improved (P < 0.05) the cecal lactobacilli. In conclusion, under the appropriate conditions of this investigation, the superzist and tested medicinal plants could undoubtedly be ideal alternatives to the use of existing antibiotics as growth promoters in poultry industry.

The effect of dietary energy and protein levels on body weight, size, and microflora of ostrich chicks.

The aim of this study was to evaluate the effect of different dietary energy and protein supplements on performance, weekly body sizes or body frame size, and microbiota of ostrich chicks during 2-9 weeks of age. Two metabolic energy levels of 2400 and 2600 kcal/kg live weight and three protein levels of 20, 22, and 24% were used. A total of 36 ostrich chickens (Struthio camelus) of the blue and black African breed were used. Body weight, 12 body measurements (i.e., circumference of the head, neck, breast, abdomen, thigh, body height, length of tail, list the other 5 here) and excretion of microbial population (Escherichia coli, Coliforms bacteria, and Lactobacillus bacteria) were measured. Mean body weight in each week of the experiment was generally the lowest when chicks were offered 2600 kcal/kg dietary energy and 24% protein. Of the 12 body measurements, the breast, abdomen, and thigh circumference and also body length were greater at the lower energy (2400 kcal/kg) and higher protein (24%) levels. Total Aerobic bacteria excretion was generally lower in response to the diet containing the higher level of energy. We conclude that ostrich chickens during 2-9 weeks of age can grow on diets that contain lower energy levels.

Probiotic level effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers.

Probiotic effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers were studied. Two hundred one-day-old male chickens were allocated to one of five treatments (four replicates of 10 birds per treatment): control, and the same control diet supplemented with 0.005%, 0.01%, 0.015% and 0.02% probiotics. Probiotics in feed at 0.01% or higher levels of supplementation improved body weight gain (+12%) and feed conversion rate (-5%) compared with the control. There were no effects on carcass traits, but the relative weights of drumsticks and wings showed increasing and decreasing linear responses, respectively, to probiotic supplementation level. Blood plasma glucose and albumin contents linearly increased (from 167.1 to 200.5 mg dl-1, and from 1.70 to 3.25 g dl-1) with increasing probiotic supplementation. Triglycerides and cholesterol contents were lower in probiotic supplemented treatments (average contents 71.3 and 125.3 mg dl-1 vs. 92.6 and 149.9 mg dl-1 in the control). Probiotics decreased cecal Escherichia coli counts, but had no effects on immunity related organs or immune response. The linear trends, either positive or negative, observed in many of the parameters studied, suggest that more studies are needed to establish the optimal concentration of probiotics in broiler feed.