Effects of proprioceptive neuromuscular facilitation and both sides up ball exercise on pain level, range of motion, muscle function after total knee arthroplasty.

Knee osteoarthritis (OA) is a degenerative joint disease caused tearing and progressive wear of articular cartilage, and total knee arthroplasty (TKA) is recommended to patients with OA. The purpose of this study was to investigate the effect of proprioceptive neuromuscular facilitation (PNF) and both sides up ball (BOSU) exercises on pain, range of motion (ROM), and muscle function in patients following TKA. Ten participants who have limitation of the knee joint from TKA were divided into two groups: the continuous passive motion (CPM)+PNF exercise group (n=5) and the CPM+BOUS exercise group (n=5). Exercise rehabilitation program consisted of PNF and BOSU exercises, and both exercises were performed twice a day for 2 weeks. To examine effect of exercise rehabilitation, visual analogue scale (VAS), sit and reach flexibility, knee ROM, and Timed Up and Go test (TUG) were measured before and after exercise intervention. A two-way repeated analysis of variance was used to confirm the main effect. If there was a significant interaction effect, an independent t-test between groups or a paired t-test between times was applied. VAS, sit and reach flexibility, knee ROM and TUG did not show interaction between the PNF and BOSU exercise groups, but all measured variables showed significant differences over time. Present findings provide information that PNF and BOSU exercise rehabilitation after TKA might be an important part of ensuring successful surgical outcomes as they have a positive impact on reducing pain, increasing ROM, improving muscle strength, and enhancing daily life movements.

Assessing the Risk of Normal Weight Obesity in Korean Women across Generations: A Study on Body Composition and Physical Fitness.

Normal weight obesity (NWO) refers to a condition in which the body mass index falls within the normal range, but the percent of body fat is excessive. Although there are reports of a high prevalence of cardiovascular and metabolic diseases in NWO, analyses regarding physical fitness have been lacking. Therefore, the purpose of this study was to analyze the age-related prevalence of NWO and to examine physical fitness across generations. Our study utilized a dataset comprising 119,835 participants for analysis. The prevalence of NWO across ages was examined using cross-tabulation analysis. For body composition and physical fitness, medians and group differences were assessed by generation through Kruskal-Wallis and Bonferroni post hoc tests. Additionally, univariate logistic regression was adopted to analyze the odds ratio. The prevalence of NWO in Korean women was 18.3%. The fat-free mass of the NWO group was consistently lower than that of both the group with normal body mass indexes (Normal) and obese body mass indexes (Obesity) across all generations. Additionally, the waist circumference and blood pressure were greater in the now group than in the Normal group. When considering maximal strength, muscle endurance, power, balance, and coordination, the NWO group exhibited lower levels compared to the Normal group. The NWO group showed lower muscle mass than both the Normal and Obesity groups, resulting in significantly reduced physical fitness compared to that of the Normal group, similar to the Obesity group. This condition may increase not only the risk of posing a potentially more serious health concern than obesity but also the risk of falls in elderly people. Therefore, based on this study, it is crucial to not only define obesity using BMI criteria but also to diagnose NWO. Public health policies and preventive measures must be implemented accordingly.

Effect of concurrent aerobic exercise and bone marrow stromal cell transplantation on time-dependent changes of myogenic differentiation-related cascades in soleus muscle after sciatic nerve injury.

The purpose of this study was to investigate the time-dependent alteration in whether concurrent aerobic exercise and bone marrow stromal cell (BMSC) engraftment could regulate myogenic differentiation-related signaling pathway in the soleus up to 35 days after sciatic nerve injury (SNI). The rats were divided as follows: the normal control (CON, n=5), sedentary group (SED, n=20), treadmill exercise group (TEX, n=20), BMSC transplantation group (BMSC, n=20), TEX+BMSC transplantation group (TEX+BMSC, n=20) 7, 14, 21, and 35 days after SNI. SNI was applied into the thigh and treadmill exercise was comprised of walking at a speed of 4 to 8 m/min for 30 min once a day. Harvested BMSC at a density of 5×106 in 50-µL phosphate-buff-ered saline was injected into the injury site. Phosphorylated (p) extracellular signal-regulated kinase 1/2 expression was dramatically upregulated in BMSC and BMSC+EX groups from 21 days after SNI compared to those in the SED group. P-ribosomal s6 kinase (RSK) was sharply increased 14 days later, and then rapidly downregulated from day 21, whereas TEX, BMSC and TEX+ BMSC groups significantly kept up expression levels of p-RSK until 35 days post injury than SED group. TEX+BMSC group significantly increased activation of protein kinase B-mammalian target of rapamycin in the soleus from day 14 and myoblast determination protein 1-myogen-in pathways was activated in TEX+BMSC group from day 21. Present findings provide information that combined intervention of aerobic exercise and BMSC transplantation might be a reliable therapeutic strategy for overcoming the morphological and functional problems in denervated soleus muscle.

The role of walking exercise on axonal regrowth and neuropathic pain markers in dorsal root ganglion after sciatic nerve injury.

The aim of this study was to determine whether walking exercise can regulate the expression level of neuropathic pain- and inflammatory response markers in the ipsilateral lumbar 4 to 6 dorsal root ganglion neurons after sciatic nerve injury (SNI). The experimental rats were randomly divided into seven groups: the normal control group, sedentary groups for 3, 7, and, 14 days postinjury (dpi), and walking exercise groups for 3, 7, and 14 dpi. Western blot techniques were used to evaluate specific neuropathic pain- and cytokine markers and mechanical allodynia was confirmed by paw withdrawal test. Mechanical allodynia was significantly improved in the walking exercise group compared to the sedentary group at all 7, 10, and 14 dpi. Furthermore, growth associated protein 43 and brain-derived neurotrophic factor levels were significantly increased in the walking exercise groups compared to the sedentary group at all 3, 7, and 14 dpi. Conversely, nuclear factor kappa-light-chain-enhancer of activated B cells, interleukin-6, tumor necrosis factor α, calcitonin gene-related peptide, and c-Fos expression levels were significantly decreased in the walking exercise groups compared to the sedentary group at all 3, 7, and 14 dpi. These findings suggest meaningful information that aggressive rehabilitation walking exercise applied early after SNI might be improve mechanical allodynia, neuropathic pain and inflammatory response markers following SNI.

The relationship between driving distance and different types of contraction in core muscles in Korea elite female golfers.

BACKGROUND: This study compared and examined the relationship between the concentric, eccentric, and isometric muscular contraction measurements that are commonly used to assess trunk strength and power, and the driving distance in elite female golfers. The purpose of this study was to utilize as a basis to evaluate the driving distance-related physical training program design and its efficacy. METHODS: Eighteen female golfers from A club in Gyeonggi province are recruited. The driving distance, medicine ball throwing, isokinetic trunk flexor, and extensor strength and power, and isometric strength in the 3D electromagnetic motion analysis system were assessed. The statistical analysis adopted the Students' t-test to adjust the different units in each assessment method, and then interrelation and correlation analysis between the driving distance was conducted. RESULTS: The result of this study is as the following: the medicine ball throwing, isokinetic trunk extensor power, isometric trunk flexor, and extensor strength, and left and right flexor and rotator strength showed high correlation with the driving distance. In the comparison between strength factors, isokinetic trunk extensor strength had a high correlation (R2=0.452), while the medicine ball throw and isokinetic trunk extensor power were reported a higher correlation in order (R2=0.740). In the overall comparison analysis, the isometric trunk left rotation, and isometric trunk extensor had an R2 value of 0.893. CONCLUSIONS: Consequently, the isometric trunk rotational strength and extensor strength had the highest correlation with the driving distance. This result can be used to design the physical fitness program for golfers.

Effect of combined intervention of exercise and autologous bone marrow stromal cell transplantation on neurotrophic factors and pain-related cascades over time after sciatic nerve injury.

The purpose of this study was to determine whether combined inter-vention of treadmill exercise and bone marrow stromal cell (BMSC) transplantation would affect the expression of neurotrophic factors in the sciatic nerve injury (SNI) and neuropathic pain-related cascades in ipsilateral lumbar 4-5 dorsal root ganglion (DRG) during the early or late stage of sciatic nerve regeneration. The rats were randomly divided into the normal control group (CONT, n=6), sedentary group (SS, n=24), exercise group (SE, n=24), BMSC transplantation group (SB, n=24), BMSC transplantation+exercise group (SBE, n=24) 1, 2, 3, and 5 weeks after SNI. Single dose of 5×106 harvested BMSC was injected into the injury area sing by a 30 gauge needle. Treadmill exercise was performed at a speed of 8 m/min for 30 min once a day. Tropomyosin-receptor kinase B, brain-derived neurotrophic factor and ciliary neurotrophic fac-tor were significantly upregulated in the SE and SBE groups at 1- and 2-week postinjury than those in the CONT and SS groups, and SB and SBE groups continuously kept up proinflammatory cytokines until the late stage of regeneration. Nuclear factor kappa-light-chain-enhancer of activated B cells, interleukin and tumor necrosis factor alpha in ipsi-lateral DRG were progressively decreased by exercise alone application and/or BMSC transplantation at early and late stage of regeneration. Present results provide reliable information that combined intervention of treadmill exercise and BMSC transplantation might be one of the effective treatment strategies for recovering sciatic nerve injury-induced neuropathic pain over time.

A comparative study on the reliability and validity of body composition results by impedance method measurement device.

The purpose of this study was to analyze the reliability and validity of the body composition results for each impedance device and use them as primary data for body composition research. Total of 58 participants, including 24 men and 34 women, were recruited. The correlation was analyzed by measuring two repetitive measurements for each device. Dual energy x-ray absorptiometry (DEXA) equipment was used as reference equipment for body composition research. All data were analyzed as IBM SPSS Statistics ver. 25.0, and the validity and reliability were estimated by calculating the timely correlation coefficient. As a result of the study, repeated measurements of the measuring instrument showed high reliability by gender and age, especially in the child age group, with relatively higher reliability than those in their 50s or older. In the validity analysis between DEXA, in the case of the male group, the validity of the fat mass amount was relatively higher than the body fat amount and body fat rate. In the case of the women's group, the validity of the body fat amount and body fat rate was relatively higher than that of the fat mass amount. The company B had the highest validity for body fat rate and body fat amount in both men and women. However, the validity for men's fat mass amount was the lowest. In subsequent studies, it is believed that studies that have expanded the population further and studies targeting special groups such as athletes should be conducted.

The timing point of exercise intervention regulates neuropathic pain-related molecules in the ipsilateral dorsal root ganglion neurons after sciatic nerve injury.

The purpose of this study was to determine whether the timing of tread-mill exercise application can control expression levels of neuropathic pain- and regeneration-related proteins in the ipsilateral lumbar 4 (L4) to 6 (L6) dorsal root ganglion cells (DRG) after sciatic nerve injury (SNI). The experimental rats were randomly divided into five groups: the normal control, SNI+sedentary (IS), exercise+SNI (EI), SNI+exercise (IE), exercise+SNI+exercise (EIE) groups. The rats in exercise groups per-formed treadmill exercise at a speed of 8 m/min for 30 min once a day during 14 days before and/or after SNI. For investigating the expression of specific neuropathic pain and regeneration-related proteins in DRG, we prepared L4 to L6 DRG in the ipsilateral side. In the quantitative analysis, growth associated protein 43 (GAP-43) and brain-derived neurotrophic factor levels were further increased in the ipsilateral DRG at all treadmill exercise groups than those in IS group. In the histological findings, GAP-43 was qualitatively increased IE and EIE groups than IS group at DRG. Wnt3a and ß-catenin were dramatically downregulated in EIE and IE groups than IS groups. In addition, nuclear factor kappa-light-chain-enhancer of activated B cells and tumor necrosis factor-α were significantly decreased in IE and EIE groups than IS group in the ipsilateral DRG. Our findings suggested novel information that regular low-intensity exercise before and/or after SNI might be a therapeutic and preventive approaches for relieving neuropathic pain and improving axonal elongation after peripheral nerve injury.

Correlation between physical efficiency index using Harvard step test and heart rate variation in college students.

The purpose of this study is to investigate the relationship between physical efficiency index (PEI) calculated by the Harvard step test and heart rate variation (HRV), and to identify parameters of HRV that can predict PEI in college students. Sixteen college students were participated in this study and they were randomly divided into two groups; higher PEI group (HPEI, n=6) and lower PEI group (LPEI, n=10). To investigate the relationship between PEI and HRV, we were measured HRV and Harvard step test. HRV test was the resting, immediately, 15 min and 30 min after the Harvard step test using electrocardiography device polyG-I. Relationship between PEI and HRV were determined Pearson correlation coefficient, and multiple regression analysis was performed for examining HRV parameters to predict PEI. As shown in the result, not only PEI was negatively correlated with root mean squared differences between adjacent normal RR intervals (RMSSD), but had a positive correlation with low-frequency/high-frequency, but also normalized low frequency (normLF), the ratio of LF, and RMSSD, the change in RR interval showed a significant difference at each time point of measurement according to PEI levels. But, there were no significant differences among the HRV variables except normLF and RMS-SD. Our findings suggest a critical information that PEI calculated by the Harvard step test can be used as an index to predict the autonomic nerve function, and high PEI may have a positive effect on changes in autonomic nerve activity during recovery after exercise intervention.

Effect of resistance circuit training on health-related physical fitness, plasma lipid, and adiponectin in obese college students.

The purpose of this study is to investigate the effects of resistance circuit training on health-related physical fitness, plasma lipid and adiponectin in obese college students. Twenty male college students participated in this study and they were randomly divided into the sedentary group (SG, n=10) and the resistance circuit training group (RCG, n=10). The exercise group underwent the resistance circuit training program for 60 min 3 times a week for 12 weeks, while the sedentary group continued activities of daily living as usual. The levels of health-related fitness, blood lipid, and adiponectin were measured twice before and after the experiment. To analyze the statistically significance of collected data, Two-way repeated measure analysis of variance was used to determine the changes in the groups over the training period. A paired t-test was used to verify the difference within each group, and an independent t-test was used to verify the difference between the groups. In this study, the body composition including body fat mass, percent body fat and body mass index were significantly decreased in RCG compared to those in SG, and the health-related fitness showed a significant difference between RCG and SG. In addition, RCG positively modulated blood concentration of total cholesterol, triglycerides, high-density and low-density lipoprotein cholesterol. However, plasma adiponectin concentration did not show any significant differences between the two groups. Therefore, the present data suggested that resistance circuit training might be regulator to improve health-related physical fitness and decrease the level of plasma lipid in obese male college students.

Effect of treadmill exercise combined with bone marrow stromal cell transplantation on atrophy-related signaling pathway in the denervated soleus muscle.

The purpose of this study was to investigate whether combination of low-intensity exercise with bone marrow stromal cell (BMSC) transplantation could regulate protein kinas B (Akt)-mammalian target of rapamycin (mTOR) and Wnt3a-ß-catenin signaling pathways for prevention of soleus muscle atrophy after sciatic nerve injury (SNI). The experimental rats divided into 5 groups (n=10): normal control group, SNI+sedentary group (SED), SNI+low-intensity treadmill exercise group (TEX), SNI+BMSC transplantation group (BMSC), SNI+TEX+BMSC transplantation group (TEX+BMSC). Sciatic nerve crush injury was applied into the middle of thigh twice for 1 min and 30 sec at interval. Low-intensity treadmill exercise was comprised of walking at a speed of 4 to 8 m/min for 30 min once a day. cultured BMSC at a density of 5×106 in 50-µL phosphate-buffered saline was injected into the distal portion of the injured sciatic nerves. TEX+BMSC group dramatically up-regulated expression levels of growth-associated protein-43 in the injured sciatic nerve at 2 weeks postinjury. Also, although Akt and mTOR signaling pathway significantly increased in TEX and BMSC groups than SED group, TEX+BMSC group showed more potent increment on this signaling in soleus muscle after SNI. Lastly, Wnt3a and the nuclear translocation of ß-catenin and nuclear factor-kappa B in soleus were increased by SNI, but TEX+BMSC group significantly downregulated activity of this signaling pathway in the nuclear cell lysate of soleus muscle. Present findings provide new information that combination of low-intensity treadmill exercise might be effective therapeutic approach on restriction of soleus muscle atrophy after peripheral nerve injury.

The effect of limitation of joint motion range due to ankle taping on the evaluation of functional motion of high school Judo athletes.

The purpose of this study is to investigate the effect of ankle injury preventative C-tape on the ankle range of motion, Y-balance test (YBT), and functional movement screening (FMS) test in Judo athletes. Participants in this study were 15 male judo athletes in high school. The angle of the ankle joint, YBT, and FMS were examined with and without the application of C-tape on each athlete's dominant foot. In YBT, ankle range of motion, and anterior reach distance were significantly lower in the taped ankle compared to the other (P<0.01). Moreover, the overhead deep squat and the FMS total score were significantly decreased after the C-tape application (P<0.05). The taping's limitation on the ankle joint range of motion may impose adverse effects on the other relevant joints, therefore a proper guideline on long-term usage is advised.

Effect of treadmill exercise and bone marrow stromal cell engraftment on activation of BDNF-ERK-CREB signaling pathway in the crushed sciatic nerve.

The effect of combined approach of exercise training and bone marrow stromal cell (BMSC) engraftment on activation of brain-derived neurotrophic factor (BDNF)-extracellular signal-regulated kinase 1 and 2 (ERK1/2)-cyclic adenosine monophosphate response element-binding protein (CREB) signaling pathway after sciatic nerve injury (SNI) was investigated. Sixty male Sprague-Dawley rats divided into the normal control, nonexercise (NEX), exercise training (EX), BMSC transplantation (TP), and exercise training+BMSC transplantation (EX+TP) groups 4 weeks after SCI. Exercise training was carried out on the treadmill device at 5-10 m/min for 20 min for 4 weeks. Single dose of 5×106 harvested BMSC was injected into the injury area of the injured sciatic nerve. In order to evaluate induction levels of BDNF-ERK1/2-CREB signaling molecules in the whole cell and nuclear cell lysates of the injured sciatic nerve, we applied Western blot analysis. BDNF was significantly increased only in EX+TP compared to NEX, EX, and TP groups. Phosphoinositide-dependent kinase-1 was more increased in EX, TP, and EX+TP groups than NEX group, but EX+TP group showed the most upregulation of phosphorylated protein kinase B compared to other groups. In addition, in the whole cell lysate, phosphorylated ERK1/2, but not activating transcription factor-3 (ATF-3) and phosphorylated CREB, was significantly increased in TP and EX+TP groups. In the nuclear cell lysate, ATF-3 and phosphorylated CREB were strongly activated in EX+TP group compared to EX group. Regular exercise training combined with BMSC engraftment would seem to be more effective in controlling activation of regeneration-related signaling pathway after SNI.

Effect of aqua walking exercise on knee joint angles, muscular strength, and visual analogue scale for patients with limited range of motion of the knee.

The purpose of this study is to identify the effects of aqua walking exercise on the joint range of motion (ROM), muscular strength, and pain in patients who have limited knee ROM from surgery or joint fixation. Ten subjects were participated in this study and divided into two groups: continuous passive motion (CPM) therapy+walking exercise on dryland (CWD) and CPM+aqua walking exercise (CAW) groups. The repeated measures analysis of variance was used to verify the difference between the groups and the duration of the program. A paired t-test was used to verify the significance of the observed difference within the groups, and an independent t-test was used to verify the significance of the observed difference between different groups. In this study, ROM in the knee flexion showed interaction effect between the two groups. CAW had a significant increase of knee flexion angle compared to those in CWD. Also, strength on the knee flexors showed interaction effect between the two groups. CAW had a significant increase of knee flexion angle compared to those in CWD, while strength on the knee extensors showed no significant interaction effect between groups. Visual analogue scale (VAS) score showed interaction between the two groups. CAW had a significant increase of the VAS scores compared to those in CWD. Given these findings reported in present study, aqua walking exercise for patients with osteoarthritis is a very safe and effective therapeutic strategies that can move the joint in the optimal ROM.

Effect of treadmill exercise on pain-related Wnt/ß-catenin signaling pathway in dorsal root ganglion neurons at the early phase regeneration of the injured sciatic nerve.

The purpose of this study was to determine whether treadmill walking exercise can improve mechanical allodynia through regulation of Wnt/ß-catenin signaling in dorsal root ganglion (DRG) neurons at the early stage of regeneration after sciatic nerve injury (SNI). The experimental rats were divided into seven groups: the normal control, sedentary groups for 3-, 7-, and 14-day post crush (dpc), and exercise group for 3, 7, and 14 dpc. The rats in exercise groups performed treadmill walking exercise at a speed of 8 m/min for 20 min once a day according to experiment duration. For evaluating neuropathic pain-like behavior after SNI, the mechanical allodynia was examined by von Frey apparatus. And the expression levels of pain-related protein were identified in the cytoplasm or nucleus of DRG neurons using Western blot techniques. Mechanical allodynia was significantly ameliorated in the exercise group at 7 and 14 dpc. Treadmill exercise further decreased Wnt3a expression at 3, 7, and 14 dpc compared to in the sedentary group. Also, phosphorylated-low-density lipoprotein receptor 6 was decreased in exercise groups at 3 and 14 dpc. Beta-catenin was significantly decreased in exercise groups at 3 and 14 dpc compared to sedentary groups as well as treadmill exercise decreased translocation of ß-catenin towards the nucleus of DRG neurons at 14 dpc. Our findings indicate that treadmill walking exercise may be an important regulator of neuropathic pain after peripheral nerve injury through delayed Wnt/ß-catenin signaling pathway in DRG neurons.

In Vitro and In Vivo Effects of Nobiletin on DRG Neurite Elongation and Axon Growth after Sciatic Nerve Injury.

Sciatic nerve injury (SNI) leads to sensory and motor dysfunctions. Nobiletin is a major component of polymethoxylated flavonoid extracted from citrus fruits. The role of nobiletin on sciatic nerve regeneration is still unclear. Thus, the purpose of this study was to investigate whether nobiletin increases DRG neurite elongation and regeneration-related protein expression after SNI. Cytotoxicity of nobiletin was measured in a concentration-dependent manner using the MTT assay. For an in vitro primary cell culture, the sciatic nerve on the middle thigh was crushed by holding twice with forceps. Dorsal root ganglion (DRG) and Schwann cells were cultured 3 days after SNI and harvested 36 h later and 3 days later, respectively. In order to evaluate specific regeneration-related markers and axon growth in the injured sciatic nerve, we applied immunofluorescence staining and Western blot techniques. Nobiletin increased cell viability in human neuroblastoma cells and inhibited cytotoxicity induced by exposure to H2O2. Mean neurite length of DRG neurons was significantly increased in the nobiletin group at a dose of 50 and 100 µM compared to those at other concentrations. GAP-43, a specific marker for axonal regeneration, was enhanced in injury preconditioned Schwann cells with nobiletin treatment and nobiletin significantly upregulated it in injured sciatic nerve at only 3 days post crush (dpc). In addition, nobiletin dramatically facilitated axonal regrowth via activation of the BDNF-ERK1/2 and AKT pathways. These results should provide evidence to distinguish more accurately the biochemical mechanisms regarding nobiletin-activated sciatic nerve regeneration.

Study on physical fitness factors affecting race-class of Korea racing cyclists.

The purpose of this study is to compare and analyze the difference of isokinetic muscle functions, anaerobic capabilities, pedaling power and maximum strength according to race-class of Korea racing cyclists. The participants in this study were 57 racing cyclist candidates who graduated from the Korea racing school. One year after graduation, we confirmed race-class of candidates and they were divided into three groups: the first-class racer group (FC, n=14), second-class racer group (SC, n=29), third-class racer group (TC, n=14). The isokinetic muscle strength of trunk and knee flexion/extension was measured using HUMAC NORM and basic physical strength such as squat and bench press was analyzed by Ariel device. As results of this study, % Fat (percentage of body fat) in FC group was the lowest compared to other groups. Isokinetic knee flexion strength was higher in FC group than SC and TC racer groups. Peak and average pedaling power as well as maximum muscle strength were significantly higher in FC group than in other groups. Our findings suggest new evidence that pedaling power and isokinetic muscle strength might be closely associated with race-class of Korea racing cyclists.

Treadmill exercise activates ATF3 and ERK1/2 downstream molecules to facilitate axonal regrowth after sciatic nerve injury.

The purpose of this study was to investigate the effect of treadmill exer-cise on activating transcription factors such as activating transcription factor 3 (ATF3) and extracellular signal-regulated kinase (ERK1/2) sig-naling pathway to facilitate axonal regrowth after sciatic nerve injury (SNI). The experimental rats divided into the normal control (n=10), sedentary groups for 7 (n=10) and 14 days (n=10) post crush, exercise group for 7 (n=10) and 14 days (n=10) post crush (dpc). The rats in ex-ercise groups run on treadmill device at a speed of 8 m/min for 20 min once a day according to exercise duration. In order to evaluate specific regeneration markers and axonal elongation in injured sciatic nerve, we applied immunofluorescence staining and western blot techniques. Treadmill exercise further increased growth-associated protein (GAP-43) expression and axonal regrowth at 7 and 14 dpc than those in sed-entary group. Among mitogen-activated protein kinase downstream molecules, phospho-ERK1/2 (p-ERK1/2) was enhanced by treadmill ex-ercise at only 7 dpc and decreased to basal level 14 days later. But c-Jun N-terminal kinase, c-Jun, and phospho-cyclic adenosine mono-phosphate response element-binding protein showed a tendency to in-crease continuously until 14 dpc by exercise. ATF3 expression in exer-cise group was upregulated at both 7 and 14 dpc compared to the sed-entary group. These results indicate that treadmill exercise had benefi-cial effect on expression of regeneration-related proteins after SNI, suggesting that exercise might be one of various therapeutic strategies for sciatic nerve regeneration.

Relationship between movement dysfunctions and sports injuries according to gender of youth soccer player.

The purpose of this study is to investigate relationship between movement dysfunctions and sports injuries according to gender of youth soccer player. Thirty-eight middle school soccer players participated in this study and they were divided into male (n=19) and female (n=19) groups. The Functional Movement Screen (FMS) and the Quadriceps-angle (Q-angle) during single-leg squat were analyzed for identifying imbalance and asymmetry of the body, and sports injury questionnaire was examined for 6 months after FMS test. The number of sports injuries did not show significant difference between youth male and female soccer athletes. In FMS results, the scores of overhead squat, hurdle step, in-line lunge, shoulder mobility, active straight leg raise, and rotary stability and the total scores were no significant differences between gender, but the score for the trunk stability push-up was significantly higher in male group than female group. There was no significant difference of Q-angle values between the left and right legs, but Q-angle value between youth male and female groups significantly showed interaction. Therefore, the present data suggested that FMS and Q-angle during single-leg squat might be indicators to predict and/or prevent sports injury in youth male and female soccer players.

Effects of treadmill exercise on activity, short-term memory, vascular dysfunction in maternal separation rats.

Maternal separation during early life causes psychiatric and neurologi-cal disorders such as anxiety and depression. Depression or anxiety is closely associated with memory impairment. The purpose of this study was to investigate the effect of treadmill exercise on activity, short-term memory, vascular dysfunction using maternal separation-induced de-pression model. Maternal separation started on 15-day-old rats. The rats in the maternal separation and fluoxetine injection group received intraperitoneal injection of 5 mg/kg of fluoxetine one time daily for 15 days from 21 to 35 days. The rats performed treadmill exercise once a day during 15 days from 21 to 35 days. There was low activity and short-term memory was decreased in the maternal separation rats. Treadmill exercise and fluoxetine injection increased activity and ameliorated memory impairment. The number of rat endothelial cells antigen-1 (RECA-1) of microvessels was decreased in the maternal separation rats. The number of RECA-1was increased by treadmill exercise and fluoxetine injection. Expression of matrix metalloproteinase-9 (MMP-9) was increased and expressions of zonula occludens-2 (ZO-2) and oc-cludin were decreased in the maternal separation rats. Treadmill exer-cise and fluoxetine injection suppressed MMP-9 expression and en-hanced ZO-2 and occludin expressions in the maternal separation rats. The present study shows treadmill exercise and antidepressant treat-ment ameliorates depressive symptom and short-term memory impair-ment by protecting from blood-brain barrier damage.