Comparison of programmed death-ligand 1 expression in adenocarcinoma and squamous cell carcinoma of the cervix in paraffin blocks of patients with cervical cancer.

AIMS: Cervical cancer (CC) is a common malignancy in women, predominantly caused by human papillomavirus. The most subtypes are adenocarcinomas (AC) and squamous cell carcinomas (SCC), which show various features and treatment responses. Programmed death-ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) as Immune checkpoint molecules, play a role in immune evasion. We investigated PD-L1 expression in AC and SCC of the cervix and explored its link to clinical characteristics. METHODS AND RESULTS: The present cross-sectional research was done between 2016 and 2022 on samples in Shahid Beheshti University of Medical Sciences-affiliated hospitals in Iran. Histological tissue samples of CCs (16 AC and 48 SCC) were assessed, and clinical information was obtained by reviewing their medical documents. PD-L1 expression was evaluated by immunohistochemistry and we used the combined positive score. SCC cases showed a higher (not significant) PD-L1 expression. The PD-L1 expression and clinical characteristics were not significantly correlated in both subgroups. CONCLUSION: Although SCC cases exhibited higher PD-L1 expression, this difference was non-significant. More investigations should highlight the role of PD-L1 in CC and the potential benefits of immunotherapy.

Prevalence and Risk Factors of Prenatal and Postnatal Depressive Symptoms in Babol Pregnancy Mental Health Registry: A Cross-Sectional Study.

BACKGROUND: Prenatal and postnatal depression (PND) is associated with adverse outcomes for mother, fetus, and child. The aim of study was to examine the prevalence and risk factors of prenatal and postnatal depressive symptoms. MATERIALS AND METHODS: This was a cross-sectional and hospital-based survey of 2305 pregnant women and post-partum women (18-48 years) that was registered in the Babol Pregnancy Mental Health Registry (BPMHR) database from June 2020 to March 2021. Two questionnaires, including demographics and depression, were analyzed in this study. Also, the Edinburg Postnatal Depression Scale (EPDS) was used to assess the depressive symptoms. Independent t test and the analysis of variance were used to compare the means. Multiple logistic regressions were used to determine risk factors for depressive symptoms. RESULTS: According to the EPDS scale, the prevalence of depressive symptoms was 19.8% in the pregnant woman group in comparison with the postpartum period (11.6%). Risk factors for antenatal depressive symptoms were parity (women with parity ≥ 4 vs. 1 parity, ß=1.808, P=0.020), two groups of gestational age (gestational age ≤12 weeks vs. 28 weeks, ß=1.562 P=0.030) as well as (gestational age 21-27 weeks vs. 28 weeks (ß=1.586, P=0.033), and high-risk pregnancy (high-risk vs. low-risk pregnancy, ß=1.457, P=0.003). For postnatal depressive symptoms, none of the factors were a significant risk. CONCLUSION: Prenatal and postnatal depressive symptoms should be screened, particularly for women in the first and second trimesters, with high parity, and those with a high-risk pregnancy, as recommended by the present study.

Placental Mesenchymal Dysplasia Associated with Severe Intrauterine Growth Restriction: A Case Report.

Placental mesenchymal dysplasia (PMD) is an uncommon placental lesion, which may mimic molar pregnancy at gross and microscopic examination. PMD can be associated with fetal growth restriction, Beckwith-Wiedemann syndrome, intrauterine fetal death, and preterm delivery. Nonetheless, it may also be associated with a normal appearing fetus. We aimed to emphasize that clinicians, radiologists, and pathologists should be aware of PMD as one of the etiologies of intrauterine growth restriction (IUGR). We presented the case of a 27-year-old gravida 1, para 1 woman who was admitted to Ayatollah Rouhani hospital, in Babol, Iran, at 30 weeks of gestation due to severe IUGR and fetal tachycardia. Ultrasound examination showed uteroplacental insufficiency and increased resistive index (RI) of umbilical artery. At last, a normal female fetus (1320 g) with no definitive anomalies was delivered by cesarean section. Pathological examination revealed cystically dilated stem villi with peripherally located thick-walled muscular stem vessels, and also stromal fibroblasts overgrowth in some stem villi. None of the examined sections revealed trophoblastic proliferation or stromal trophoblastic inclusion. The findings confirmed the diagnosis of PMD. Careful radiological and pathological examination should be performed in the case of IUGR for ruling out the rare placental abnormalities, including PMD.

Preparation of a novel magnetic Pd(II) ion-imprinted polymer for the fast and selective adsorption of palladium ions from aqueous solutions.

A novel magnetic ion-imprinted polymer with high accessibility to palladium ions was synthesized via co-precipitation polymerization. Accordingly, a ternary complex composed of PdCl2 as an imprinting ion, 8-aminoquinoline (AQ) as a ligand, and 4-vinyl pyridine (4-VP) as a complexing monomer was applied to Fe3O4@SiO2 as magnetic core, followed by precipitation polymerization using 2-hydroxyethyl methacrylate (2-HEMA) as a co-monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker, and 2,2-azobisisobutyronitrile (AIBN) as an initiator in the presence of 2-methoxyethanol as a solvent. The palladium ions were leached out by a solution containing 50% (v/v) HCl. The synthesized polymer was characterized physically and morphologically using different techniques. In order to assess the conditions required for adsorption, as well as the selectivity and reusability, batch adsorption experiments were carried out. The experiments exhibited that the maximum adsorption capacity was about 65.75 mg g-1 at 25 °C, while the pH solution and the adsorbent dose were 4 and 1 g L-1, respectively. Kinetic studies of experimental data demonstrated that they correspond very much to the pseudo-second-order kinetic model. The development of the Langmuir and Freundlich isothermal models on the equilibrium data proved to correspond well to the Langmuir isotherm model. Interferences studies of the magnetic polymer demonstrated higher affinity and discernment for palladium ions than other co-existing ions in the solutions. Spontaneous (ΔG < 0) and exothermic (ΔH < 0) behavior of the adsorption process is confirmed by thermodynamic studies. In addition, the affinity of the spent polymer has not been dramatically reduced over at least five regeneration cycles.