RESUMEN
Eosinophilic lung diseases (ELD) are a variety of several clinical entities, which may result from different etiologies, including drug treatment. Dapsone, a sulfone antibiotic widely used in leprosy (among other indications), has been described as a possible cause of ELD. We report a patient with leprosy who presented with respiratory symptoms and pulmonary infiltrates and was diagnosed as suffering from eosinophilic pneumonia. To the best of our knowledge, this is the first report in which the diagnosis of dapsone-induced eosinophilic pneumonia was supported by bronchoalveolar lavage, lung biopsy and typical response to therapy.
Asunto(s)
Dapsona/efectos adversos , Leprostáticos/efectos adversos , Eosinofilia Pulmonar/inducido químicamente , Eosinofilia Pulmonar/patología , Anciano , Biopsia , Clofazimina/uso terapéutico , Humanos , Lepra Lepromatosa/tratamiento farmacológico , Masculino , Eosinofilia Pulmonar/fisiopatología , Rifampin/uso terapéuticoAsunto(s)
Factores Inmunológicos/uso terapéutico , Omalizumab/uso terapéutico , Trastornos por Fotosensibilidad/tratamiento farmacológico , Luz Solar/efectos adversos , Urticaria/tratamiento farmacológico , Niño , Humanos , Factores Inmunológicos/efectos adversos , Masculino , Omalizumab/efectos adversos , Trastornos por Fotosensibilidad/diagnóstico , Trastornos por Fotosensibilidad/etiología , Índice de Severidad de la Enfermedad , Resultado del Tratamiento , Urticaria/diagnóstico , Urticaria/etiologíaRESUMEN
The RecE recombination pathway is active in Escherichia coli recB recC sbcA mutants. To isolate and characterize products and intermediates of RecE-mediated, break-induced, intramolecular recombination, we infected recB recC sbcA mutants, expressing EcoRI endonuclease, with chimeric lambda phages that allow EcoRI-mediated release of cloned linear recombination substrates. Substrates with direct terminal repeats recombined to yield a circular product with one copy of the repeated sequence. Some recombinants were heteroallelic for the recombining markers. Markers distant to the break were recovered in the circular product at a higher frequency than markers close to the break. To examine the heteroduplex structures that may have yielded the heteroallelic recombinants, nonreplicative substrates were employed. Some of the nonreplicative recombination products contained heteroduplexes, with a strong bias for paired strands ending 3' at the break. This strand bias in heteroduplex formation is consistent with recombination models that postulate homologous pairing of protruding 3' single-stranded ends.
Asunto(s)
ADN Bacteriano/genética , Escherichia coli/genética , Ácidos Nucleicos Heterodúplex/genética , Recombinación Genética , Secuencia de Bases , Enzimas de Restricción del ADN , Genes Bacterianos , Marcadores Genéticos , Modelos Genéticos , Datos de Secuencia Molecular , Mutación , PlásmidosRESUMEN
To test the double-strand break (DSB) repair model in recombination by the RecE pathway of Escherichia coli, we constructed chimeric phages that allow restriction-mediated release of linear plasmid substrates of the bioluminescence recombination assay in infected EcoRI+ cells. Kinetics of DSB repair and expression of recombination products were followed by Southern hybridization and by the bioluminescence recombination assay, respectively. Plasmid recombinants were analyzed with restriction endonucleases. Our results indicate that a DSB can induce more than one type of RecE-mediated recombination. A DSB within the homology induced intermolecular recombination that followed the rules of the DSB repair model: (1) Recombination was enhanced by in vivo restriction. (2) Repair of the break depended on homologous sequences on the resident plasmid. (3) Break-repair was frequently associated with conversion of alleles that were cis to the break. (4) Conversion frequency decreased as the distance from the break increased. (5) Some clones contained a mixture of plasmid recombinants as expected by replication of a heteroduplex in the primary recombinant. The rules of the DSB repair model were not followed when recombination was induced by a DSB outside the homology. Both the cut and the uncut substrates were recipients in conversion events. Recombination events were associated with deletions that spanned the break site, but these deletions did not reach the homology. We propose that a break outside the homology may stimulate a RecE-mediated recombination pathway that does not involve direct participation of DNA ends in the homologous pairing reaction.
Asunto(s)
Reparación del ADN/genética , Proteínas de Escherichia coli , Escherichia coli/genética , Exodesoxirribonucleasas/metabolismo , Plásmidos/genética , Recombinación Genética/genética , Southern Blotting , Daño del ADN/genética , Cinética , Mapeo Restrictivo , Homología de Secuencia de Ácido NucleicoRESUMEN
The phospholipid inflammatory mediator platelet activating factor (PAF) has been shown to stimulate certain functions of polymorphonuclear leukocytes (PMN). However, the effect of PAF on surface complement receptors of PMN has not been described. Using monoclonal antibodies and flow cytometry, we have assessed the effects of PAF on surface expression of membrane receptors for C3bi (CR3) and C3b (CR1) in human PMN. PAF (optimal concentration of 1 x 10(-8) M) increased CR3 190% and CR1 174% compared with unstimulated cells at 37 degrees C, while the PAF analogue lyso-PAF had no stimulatory effect. Both CR3 and CR1 responses to PAF reached maximum levels at 15-30 min. PAF effects were comparable to peak effects induced by LTB4 but less than induced by FMLP. A PAF receptor antagonist, SRI 63-441, blocked the increased complement receptor expression in a dose-dependent manner with maximal inhibition of 80-95% at 5 x 10(-6) M. Extracellular calcium had no effect on CR1 expression but slightly enhanced and EGTA partially inhibited the PAF-induced increase in CR3 expression. Simultaneous incubation with PAF and LTB4 enhanced CR3 and CR1 expression more than either agent alone. These findings indicate that PAF, alone and in combination with LTB4, can induce altered expression of complement receptors on the surface of PMN. This effect may enhance adhesion and phagocytosis by PMN at inflammatory reaction sites.
Asunto(s)
Neutrófilos/efectos de los fármacos , Factor de Activación Plaquetaria/farmacología , Receptores de Complemento/fisiología , Calcio/fisiología , Relación Dosis-Respuesta a Droga , Humanos , Leucotrieno B4/farmacología , Neutrófilos/inmunología , Receptores de Complemento/análisis , Receptores de Complemento/efectos de los fármacos , Receptores de Complemento 3bRESUMEN
Four patients developed nonthrombocytopenic purpura two to three weeks after initiation of quinidine therapy. The skin lesions disappeared and did not recur after cessation of quinidine therapy. Histologic examination revealed leukocytoclastic vasculitis with deposition of C3, IgA, and/or IgM in the small dermal vessels. Since quinidine purpura is usually associated with thrombocytopenia, the possibility of leukocytoclastic vasculitis as an additional cause of purpura is stressed.
Asunto(s)
Quinidina/efectos adversos , Vasculitis Leucocitoclástica Cutánea/inducido químicamente , Anciano , Femenino , Humanos , Pierna , Masculino , Púrpura/inducido químicamente , Púrpura/patología , Piel/patología , Vasculitis Leucocitoclástica Cutánea/patologíaRESUMEN
Q fever, caused by Coxiella burnetii, can present as an outbreak of acute disease ranging from asymptomatic disease, pneumonia, hepatitis or fever of unknown origin, which can progress to a chronic disease, most frequently endocarditis. The occurrence of Q fever within families is rarely described, and in most cases presents with uniform acute disease manifestations. Here we present a familial cluster of Q fever presenting as highly variable synchronous manifestations in four of five family members, including prolonged fever of unknown origin, asymptomatic carrier state, hepatitis, and chronic endocarditis developing in the absence of previous symptoms. This case series highlights the possibility of Q fever developing in cohabitated individuals with highly variable symptoms masking the common disease etiology. Screening of all exposed individuals, even those not clinically suspected to be infected, may enable to better identify, treat and prevent progression to chronic disease.
Asunto(s)
Coxiella burnetii/aislamiento & purificación , Salud de la Familia , Fiebre Q/epidemiología , Fiebre Q/patología , Adolescente , Adulto , Análisis por Conglomerados , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Chronic granulomatous disease (CGD) is a rare recessive disorder caused by defects in the NADPH oxidase enzyme complex of phagocytes (neutrophils, eosinophils and monocytes). CGD phagocytes fail to produce superoxide and other reactive oxygen species following cell activation (Malech, 1993). The products of oxidase activation can be measured in individual cells by flow cytometry using specific fluorescent probes that increase fluorescence upon oxidation (Trinkle et al., 1987). This approach can be used to confirm a diagnosis of CGD, and to detect the normal/abnormal phagocyte mixture that characterizes the X-linked CGD carrier state. Three fluorescent probes have been described as useful for this purpose: 2'7'-dichlorofluorescin diacetate (DCF) (Bass et al., 1983), 5,6-carboxy-2'7'-dichlorofluorescein diacetate, bis(acetoxymethyl) ester (C-DCF) (Hockenbery et al., 1993) and dihydrorhodamine 123 (DHR) (Rothe et al., 1988; Kinsey et al., 1987). A direct comparison between these three probes has not been reported. In this study we performed a direct comparison between these three probes, evaluating their ability in flow cytometric analysis to maximize fluorescent separation between activated CGD patient and normal granulocytes. Using a whole blood technique with phorbol myristate acetate (PMA) as an activator, it was found that DHR loaded normal granulocytes had a fluorescence intensity which, upon activation, was 48-fold higher than that of C-DCF loaded granulocytes and seven-fold higher than DCF loaded granulocytes (P < 0.001). Use of sodium azide to decrease the catabolism of H2O2 enhanced the fluorescence of DCF by 140%, C-DCF by 45% and DHR by 25%, suggesting that DCF is primarily sensitive to H2O2. DCF and DHR were then evaluated for sensitivity in the detection of small percentages of normal cells in a CGD/normal granulocyte mixture. Normal sub-populations as small as 0.1% could clearly be distinguished using DHR, while DCF was insensitive at this level. Based on these findings, we used DHR in an effort to detect normal granulocytes in a CGD patient following therapeutic granulocyte transfusion. We were able to detect normal granulocytes in the circulation for up to 18 h after transfusion. With these data we show that DHR is the most sensitive flow cytometric indicator for the detection of oxygen reactive species in activated granulocytes and is the best probe for evaluating CGD patients and carriers. In addition, our data suggest that DHR is a useful tool for monitoring circulating normal granulocytes in CGD patients following transfusion, and potentially will be a sensitive probe for assessing the success of such future technologies as gene therapy for CGD.
Asunto(s)
Citometría de Flujo/métodos , Colorantes Fluorescentes , Granulocitos/metabolismo , Enfermedad Granulomatosa Crónica/metabolismo , Estallido Respiratorio , Fluoresceínas , Enfermedad Granulomatosa Crónica/diagnóstico , Humanos , Transfusión de Leucocitos , RodaminasRESUMEN
Brain mast cells (MC) are located in close proximity to glial cells and it has been suggested that they belong to the connective tissue phenotype. To determine whether the local microenvironment provided by glial cells can influence mouse bone marrow-derived MC (BMMC), the putative counterpart of mucosal MC, we co-cultured these two cell types. BMMC numbers, morphology, histochemical properties and histamine content as well as glial cell morphology and function were evaluated up to 21 days. Our data indicate that BMMC adhere, proliferate, survive and can be activated to release histamine on the glial cell monolayers without changing their phenotype. Co-cultured glial cells preserve their morphological appearance and function throughout the culture period. These data indicate that central nervous system (CNS) glial cells do not induce phenotypic changes in BMMC and do not interfere with their viability and function.
Asunto(s)
Comunicación Celular , Mastocitos/fisiología , Neuroglía/fisiología , Animales , Células Cultivadas , Liberación de Histamina , Interleucina-3/fisiología , Ratones , Ratones Endogámicos BALB CRESUMEN
The potential role of donor's mature T lymphocytes on the recovery of various immunological functions and hematopoiesis was investigated in lethally irradiated BALB/c mice by studying reconstitution with normal, as compared with T-cell-depleted, syngeneic marrow grafts. Recovery of total, as well as mononuclear, peripheral white blood cell counts, platelets, hemoglobin levels, proportion of Thy 1.2+ cells, responses to concanavalin A, phytohemagglutinin and lipopolysaccharide, mixed lymphocyte response, cell-mediated lympholysis response, anti-SRBC agglutinins and natural killer activity were basically similar in recipients of unmanipulated (as compared with T cell depleted) syngeneic marrow grafts. The data suggest that in a syngeneic murine bone marrow transplantation setting, mature donor T lymphocytes do not seem to play a major role in immunohemopoiesis. Normal T cell number and T-cell-dependent immune function can be readily regenerated out of the stem cell reservoir of adult donors following transplantation into lethally ablated recipients.
Asunto(s)
Trasplante de Médula Ósea , Animales , Recuento de Eritrocitos , Femenino , Células Asesinas Naturales/fisiología , Recuento de Leucocitos , Prueba de Cultivo Mixto de Linfocitos , Depleción Linfocítica , Ratones , Ratones Endogámicos BALB C , Linfocitos T/inmunología , Irradiación Corporal TotalRESUMEN
Graft-versus-host disease (GVHD), a serious complication of allogeneic bone marrow transplantation (BMT), can be prevented by in vitro depletion of T cells from the bone marrow (BM) prior to transplantation. The purpose of this study was to assess the role of BMT cells in the reconstitution of various immune functions following BMT across minor histocompatibility barriers. Lethally irradiated CBA/J (H-2k) mice were grafted with either 10(7) unseparated or T-cell-depleted BM cells from B10.BR (H-2k, minor-histoincompatible) mice. Blood counts, BM colonies in agar, and various immune functions of spleen cells from the recipient mice were tested 2-12 weeks post-BMT and compared with those of normal donors. The following observations were made: (A) Peripheral blood lymphocyte counts decreased to 30% of normal 2 weeks post-BMT with almost normal recovery at 8 weeks. (B) The percentage of Thy1.2+ splenocytes reached normal levels at 8 weeks post-BMT. (C) The number of BM colonies (GM-CFU) was reduced to 10% at 2 weeks and fully recovered at 12 weeks. (D) Proliferative response to the B-cell mitogen LPS was fully reconstituted after 4 weeks; however, anti-SRBC PFC (following Mishell-Dutton cultures) was restored 50% at 8-12 weeks. (E) Reconstitution of T cell functions including proliferative responses to concanavalin A, phytohemagglutinin, and allogeneic leukocytes, and allocytotoxicity, did not exceed 50% even 12 weeks post-BMT. Overall, depletion of T cells from donor BM allografts incompatible at minor histocompatibility loci, did not seem to significantly alter the rate of immunohematopoietic reconstitution in the lethally irradiated BM recipients.
Asunto(s)
Trasplante de Médula Ósea , Sitios Menores de Histocompatibilidad , Linfocitos T , Animales , Células de la Médula Ósea , Ensayo de Unidades Formadoras de Colonias , Femenino , Hematopoyesis , Prueba de Cultivo Mixto de Linfocitos , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos CBA , Mitógenos/farmacología , Irradiación Corporal TotalRESUMEN
This study was undertaken to determine whether zinc, manganese and copper could regulate the thrombin-induced secretion of the granule-associated mediator, beta-hexosaminidase, from mast cells derived from mouse bone marrow. Exposure of thrombin to copper (2-100 microM) does not affect the enzyme-induced release of beta-hexosaminidase from the mast cells. Zinc at 50 microM reduced the degranulation of calcium ionophore A23187 activated cells by 75% and that of immunological challenge or thrombin by 30% each. Exposure of the thrombin to incremental concentrations of manganese (2-100 microM) prevents its degranulation activity in a dose-related fashion. 75% inhibition of the enzyme activity was achieved at 100 microM manganese. However, exposure of IgE sensitized or unsensitized cells to incremental concentrations of manganese (2-400 microM) prior to antigen or calcium ionophore A23187 stimulation, does not significantly affect the exocytosis of beta-hexosaminidase. The binding of purified human FITC-thrombin to E-mast cells was analyzed by fluorescence flow cytometry. All cells bound specifically the labelled thrombin. Pretreatment of the FITC-thrombin with 100 micron zinc or manganese had no effect on the binding of the labelled thrombin to the cells. It was assumed that manganese modulates either directly the thrombin activity or the substrate for the enzyme on the cell surface.
Asunto(s)
Hexosaminidasas/metabolismo , Manganeso/farmacología , Mastocitos/metabolismo , Trombina/metabolismo , Zinc/farmacología , Animales , Células de la Médula Ósea , Calcimicina/farmacología , Diferenciación Celular , Células Cultivadas , Cobre/farmacología , Exocitosis/efectos de los fármacos , Citometría de Flujo , Inmunoglobulina E/inmunología , Masculino , Ratones , Ratas , Tasa de Secreción/efectos de los fármacos , beta-N-AcetilhexosaminidasasRESUMEN
Left main coronary artery (LMCA) stenosis occurs in 10% of patients undergoing coronary arteriography, but total occlusion is rare. Goldberg et al reported 6 cases of complete obstruction of the LMCA among 2,200 patients studied arteriographically. Sudden obstruction of the LMCA should be lethal, and we found no report describing survival with sudden obstruction of the LMCA. The present report describes such a patient.
Asunto(s)
Angiografía Coronaria , Enfermedad Coronaria/diagnóstico por imagen , Enfermedad Coronaria/diagnóstico , Muerte Súbita , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Inhalation of hydrogen gas and its immediate diffusion into the blood permits an accurate and reproducible determination of its clearance through a fine platinum electrode inserted in the renal cortex. The oxidation of molecular hydrogen at the surface of the electrode generates a current which is then easily recorded. Such recordings accurately reflect regional arterial circulation. Experiments have been done in rats to determine regional renal cortical arterial circulation in (1) normal conditions, (2) immediately following unilateral, complete ureteral obstruction, and (3) following a twenty-four-hour ureteral obstruction. Hundreds of simple and reproducible recordings were obtained. Although acute obstruction does not immediately affect cortical blood supply, a twenty-four-hour obstruction was accompanied by a 31.3 per cent reduction in circulation.
Asunto(s)
Hidrógeno , Circulación Renal , Animales , Electrodos Implantados , Corteza Renal/irrigación sanguínea , Platino (Metal) , Ratas , Ratas Endogámicas , Factores de Tiempo , Obstrucción Ureteral/etiología , Obstrucción Ureteral/fisiopatologíaRESUMEN
We describe herein the clinical and laboratory manifestations of a unique group of patients (pts) presenting with hypereosinophilic syndrome (HES) who were treated in our medical centers for 4-13 years. Skin biopsies, flow cytometry of peripheral blood mononuclear cells (PBMC), assays for cytokines and immunoglobulin (Ig) production in vitro, and Southern blots of T-cell receptor (TCR) genes were performed. All four pts had a persistent hypereosinophilia (> 1.9 x 10(9)/L) and chronic skin rash. Three of four had elevated IgE, thrombotic manifestations and lung involvement (asthma and/or infiltrates), and one had deforming sero-negative arthritis of the hands. 66-95% of their peripheral T-cells expressed CD4 but not CD3 or TCR molecules on the cell surface membrane. Activated CD4+CD3- cells secreted interleukin (IL)-4 and/or 5, and were required for maximal IgE secretion by autologous B-cells. Two pts had evidence of rearrangement of TCR genes of the CD4+CD3- cells, one of whom died of anaplastic lymphoma. In conclusion, HES with CD4+CD3- lymphocytosis may be associated with high serum IgE, dermatological, pulmonary, thrombotic and rheumatic manifestations which may be due to Th2 effects of CD4+CD3- cells migrating to end organs. Fatal systemic lymphoid malignancy may also develop in some pts with monoclonal expansion of the CD4+CD3- T-cells.
Asunto(s)
Complejo CD3/análisis , Síndrome Hipereosinofílico/sangre , Células Th2/patología , Adulto , Anciano , Antígenos CD4/análisis , Células Clonales/inmunología , Células Clonales/patología , Femenino , Reordenamiento Génico de Linfocito T , Humanos , Síndrome Hipereosinofílico/complicaciones , Síndrome Hipereosinofílico/etiología , Inmunoglobulina E/sangre , Inmunofenotipificación , Linfocitosis/sangre , Linfocitosis/complicaciones , Linfocitosis/inmunología , Masculino , Persona de Mediana Edad , Células Th2/inmunologíaRESUMEN
Five patients developed nasal visual field defects as a result of involvement of the intracranial portion of the optic nerves. The cause in each patient, respectively, was as follows: (1) dolichoectatic carotid arteries, (2) optochiasmatic arachnoiditis, (3) meningioma of the olfactory groove, (4) pituitary apoplexy, and (5) pituitary chromophobe adenoma. The common factor in these cases was probably impaired circulation in the prechiasmal arterial anastomotic network. The nasal visual field loss present in these cases was characterized by a pattern similar to that seen in glaucoma but with impairment of visual acuity. The superior nasal visual field was usually normal and the lower temporal visual field often defective.
Asunto(s)
Encefalopatías/complicaciones , Enfermedades del Nervio Óptico/diagnóstico , Campos Visuales , Adenoma Cromófobo/complicaciones , Adolescente , Adulto , Anciano , Aracnoiditis/complicaciones , Enfermedades de las Arterias Carótidas/complicaciones , Neoplasias de los Nervios Craneales/complicaciones , Femenino , Humanos , Masculino , Neoplasias Meníngeas/complicaciones , Meningioma/complicaciones , Persona de Mediana Edad , Nervio Olfatorio/cirugía , Quiasma Óptico/cirugía , Enfermedades del Nervio Óptico/etiología , Enfermedades de la Hipófisis/complicaciones , Neoplasias Hipofisarias/complicacionesRESUMEN
Mast cells (MC) have been implicated in the pathogenesis of experimental allergic encephalomyelitis (EAE). In order to further evaluate their role, several morphological and functional studies were performed. Semiquantitative counts of histological sections showed a significant reduction in MC numbers in EAE brains. In addition, a higher proportion of EAE MC (about 50-70%) appeared degranulated compared with about 20% degranulation in controls. Central nervous system (CNS) MC exhibited staining properties of connective tissue MC and about 98% of them, both in diseased and control rats, were located in the thalamus. They were not present in the spinal cord and did not relate to EAE lesions. In vitro incubation of peritoneal MC (of connective tissue phenotype) with either MBP, or with neuropeptides such as substance P or bradykinin resulted in release of beta-hexosaminidase and histamine. The latter responses were similar in both EAE and control rats. It is suggested that the decrease in number and in granular content of CNS MC in EAE may reflect prior in vivo activation. The fact that MC were activated by MBP and by neuropeptides in vitro suggests a possible mechanism of MC activation in EAE.
Asunto(s)
Sistema Nervioso Central/patología , Encefalomielitis Autoinmune Experimental/patología , Mastocitos/patología , Proteína Básica de Mielina/farmacología , Neuropéptidos/farmacología , Animales , Bradiquinina/farmacología , Gránulos Citoplasmáticos/metabolismo , Femenino , Mastocitos/efectos de los fármacos , Mastocitos/metabolismo , Neuroinmunomodulación , Cavidad Peritoneal/citología , Ratas , Ratas Endogámicas Lew , Sustancia P/farmacología , Tálamo/patología , beta-N-Acetilhexosaminidasas/metabolismoRESUMEN
A case of spontaneous subdural hematoma in the cervicothoracic region associated with a small meningioma in a patient on anticoagulant therapy is presented. The neurological complications of anticoagulant therapy are discussed briefly. Progressive neurological deterioration in a patient on anticoagulant therapy should prompt the performance of an emergency myelogram and a possible laminectomy in spite of the potential risks of these procedures. Intraspinal bleeding occurs more frequently in the form of an epidural hematoma, but the clinical presentation may not allow differentiation from a subdural hematoma. The possible causal relation between the asymptomatic spinal meningioma, the anticoagulant therapy, and the formation of the subdural hematoma is discussed.
Asunto(s)
Hematoma Subdural/complicaciones , Neoplasias Meníngeas/complicaciones , Meningioma/complicaciones , Neoplasias de la Médula Espinal/complicaciones , Warfarina/efectos adversos , Femenino , Hematoma Subdural/inducido químicamente , Hematoma Subdural/patología , Humanos , Neoplasias Meníngeas/patología , Meningioma/patología , Persona de Mediana Edad , Neoplasias de la Médula Espinal/patologíaRESUMEN
The widespread usage of ventriculoperitoneal shunts has been followed by a plethora of complications. One of these complications, the separation and migration of the distal tubing to be free intraperitoneally, has been relatively disregarded in the literature both as a phenomenon and as to treatment. We present our experience with nine such cases, four involving two peritoneal catheters. Unless contraindicated, we think that such tubing should be removed during shunt revision by single digit blind palpation of the tubing. No complications arose from this procedure, and the postoperative course was uneventful. The indications for this procedure are discussed in relation to the relevant literature.
Asunto(s)
Derivaciones del Líquido Cefalorraquídeo/métodos , Derivaciones del Líquido Cefalorraquídeo/efectos adversos , Niño , Preescolar , Falla de Equipo , Humanos , Lactante , Cavidad Peritoneal , ReoperaciónRESUMEN
Subarachnoid hemorrhage (SAH) was induced in 13 adult mongrel cats by a slow injection of fresh autogenous blood into the cisterna magna. Serial determinations of regional cerebral blood flow (rCBF) in the cortex and deep-seated areas (internal capsule, thalamus, and caudate nucleus) were made during the following 2 hours, while intracranial pressure (ICP) was maintained at normal values. A decrease in rCBF was observed in all the areas examined. This reduction followed a characteristic triphasic pattern with an initial steep decline immediately after the SAH. The clinical implications of these findings are discussed.