RESUMEN
Objective: To study the clinical features and prognostic impact of transarterial chemoembolization (TACE), immune checkpoint inhibitors (ICIs), and tyrosine kinase inhibitors (TKIs) combination therapy regimens in the treatment of patients with hepatitis B virus-related intermediate-and advanced-stage hepatocellular carcinoma with secondary cholestasis. Methods: Patients with HBV-related intermediate-and advanced-stage hepatocellular carcinoma (HBV) who visited the Affiliated Hospital of Xuzhou Medical University between January 1, 2020, and December 31, 2022, were enrolled. TACE+TKIs +ICIs combination therapy was used to treat all patients. The occurrence and factors influencing cholestasis, as well as the impact on prognosis after combined therapy, were analyzed. The measurement data were compared using a t-test and a non-parametric rank sum test. The count data was compared using the χ(2) test. The survival rates were compared using a log-rank test between different groups. Results: A total of 106 cases with HBV-related intermediate-and advanced-stage hepatocellular carcinoma were enrolled. The probabilities of secondary cholestasis within 3 and 6 months, 1, 2, and 3 years after TACE+ICIs+TKIs combination therapy were 9.4%, 12.3%, 14.2%, 24.5%, and 24.5%, respectively. Patients with secondary cholestasis had persistent symptoms and rapid progression. During the treatment course, the median survival time was significantly longer in patients with hepatocellular carcinoma without secondary cholestasis than that of patients with cholestasis (26.9 months vs. 13.7 months, respectively, P < 0.05). Secondary cholestasis, baseline aspartate aminotransferase, and prothrombin activity levels were independent risk factors that affected the survival and prognosis of patients treated with combination therapy. There was no statistically significant difference in the occurrence of other adverse reactions between the two groups with secondary and non-secondary cholestasis during the treatment course (47.5% vs. 43.3%, χ(2)=0.058, P = 0.810). Conclusion: TACE+ICIs+TKIs therapy combination is relatively common in the treatment of patients with HBV-related intermediate-and advanced-stage hepatocellular carcinoma with secondary cholestasis. Moreover, accelerated disease progression is an independent risk factor affecting the survival and prognosis of patients.
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Carcinoma Hepatocelular , Quimioembolización Terapéutica , Colestasis , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/patología , Neoplasias Hepáticas/patología , Pronóstico , Virus de la Hepatitis B , Estadificación de Neoplasias , Estudios Retrospectivos , Inmunoterapia , Colestasis/etiología , Resultado del TratamientoRESUMEN
Objective: To investigate the effect of high-fat diet (HFD) on respiratory function and its mitochondrial mechanism in mice. Methods: Twenty 4-week-old healthy male C57BL/6 mice were divided by simple random grouping into two groups, 10 mice for each group. Mice were raised with normal diet (NFD) and HFD for 16 weeks, respectively, and weighed once every two weeks. At the end of the intervention, respiratory parameters were measured by whole-body plethysomography, serum and diaphragm tissue lipid species were measured, diaphragm tissue was stained to observe diaphragmmatic morphology, muscle fiber phenotype and mitochondrial ultrastructure. Real-time fluorescence quantitative PCR and immunoblotting were used to detect myosin heavy chain(MHC) and mitochondrial dynamics-related genes and protein expression. Results: The baseline body weights of NFD and HFD mice were (19.17±0.59) and (19.12±0.64) g, respectively, with no statistically significant differences (P=0.857). After 16 weeks of feeding, the body weight of mice in the HFD group was (41.28±2.21) g, which was higher than NFD group [(27.14±0.53) g, P<0.001]. The peak inspiratory flow rate, tidal volume and minute ventilation of mice in the HFD group were (5.72±0.64) ml/s, (0.23±0.04) ml and (97.49±21.68) ml, respectively, which were lower than NFD group [(7.70±1.52) ml/s, (0.31±0.07) ml and (129.99±28.87) ml, respectively, all P values<0.05]. Penh value was 1.16±0.07, which was higher than NFD group (0.98±0.09, P<0.001). The diaphragmatic triglyceride content of mice in the HFD group was (20.43±6.36) mmol/mg, which was higher than NFD group [(11.62±1.78) mmol/mg] (P=0.003), and lipid droplets were deposited in the diaphragm fibers. The percentage of MHC-â muscle fibers in the diaphragm of mice in the HFD group was 13.33%±2.95%, which was lower than NFD group (19.20%±1.23%, P=0.034). The mitochondria of the diaphragm in the NFD group were arranged in rows with clear structures, while in the HFD group showed swelling, cristae breakage and vacuoles. The relative expression level of mitochondrial fusion protein 2 in the diaphragm of the HFD group was 0.61±0.16, which was lower than NFD group (1.28±0.03, P<0.001); the relative expression of mitochondrial dynamin-related protein 1 were 1.18±0.06 and 0.91±0.11, respectively, both higher than NFD group (0.61±0.07 and 0.60±0.04, respectively, P<0.001). Conclusion: HFD impairs respiratory function in mice by the mechanism associated with the MHC-â muscle fibers decrease and the mitochondrial dynamics imbalance of diaphragm.
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Diafragma , Dieta Alta en Grasa , Animales , Peso Corporal , Masculino , Ratones , Ratones Endogámicos C57BL , MitocondriasRESUMEN
The dorsal cortex of the pond turtle (Pseudemys scripta) is a relatively simple structure consisting of two principal classes of neurons that occupy three distinct layers. Morphological, pharmacological, and physiological data suggest many similarities to the mammalian neocortex, rendering it an interesting preparation for comparative studies. We prepared monoclonal antibodies to the turtle dorsal cortex by immunizing mice with cortical tissue from adult turtles. Twelve antibodies were generated that recognize specific components of the turtle cortex. Among these, eight antibodies label only neurons and four label only ependymal glial cells. Differences in tissue staining pattern and immunoglobulin class suggest a heterogeneity of antigenic specificity among the antibodies. The staining patterns of three of our antibodies are described. TC3, like all other neuron-marking antibodies generated, labels a subset of both pyramidal and stellate cell types. It also cross-reacts with a subset of mammalian cortical neurons and labels them with a pattern similar to that observed in the turtle cortex. TC5 stains ependymal cells and their glial processes in the turtle cortex, and cross-reacts with fibrous astrocytelike processes in mammalian neocortical white matter. TC9 appears to recognize antigens of neurons sharing a pallial derivation in turtle.
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Corteza Cerebral/citología , Neuronas/clasificación , Tortugas/anatomía & histología , Animales , Anticuerpos Monoclonales , Corteza Cerebral/inmunología , Reacciones Cruzadas , Epéndimo/citología , Epéndimo/inmunología , Epítopos/inmunología , Haplorrinos/anatomía & histología , Haplorrinos/inmunología , Ratones , Neuroglía/citología , Neuroglía/inmunología , Especificidad de la Especie , Tortugas/inmunologíaRESUMEN
In order to learn more about the anatomical substrate for gamma-aminobutyric acid (GABA)-mediated inhibition in cortical structures, the intrinsic neuronal organization of turtle dorsal cortex was studied by using Golgi impregnation, immunohistochemical localization of GABA and its synthetic enzyme glutamic acid decarboxylase (GAD), and histochemical localization of the presynaptic GABA-degrading enzyme GABA-transaminase (GABA-T). GABAergic markers are found in neurons identical in morphology and distribution to Golgi-impregnated aspiny and sparsely spiny nonpyramidal neurons with locally arborizing axons and appear to label most if not all of the nonpyramidal neurons. In addition, the GABAergic markers are found in punctate structures in a distribution characteristic of presumed inhibitory terminals. The spine-laden pyramidal neurons, the principal projecting cell type in the dorsal cortex, are devoid of labelling for GABAergic markers but are surrounded by presumed GABAergic terminals. The data complement previous physiological and ultrastructural studies that implicate aspiny and sparsely spiny nonpyramidal neurons as mediators of intrinsic inhibition of pyramidal neurons in turtle cortex. The results also suggest similarities in the functional organization of intrinsic inhibitory elements in turtle and mammalian cortex.
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4-Aminobutirato Transaminasa/análisis , Corteza Cerebral/análisis , Glutamato Descarboxilasa/análisis , Tortugas/metabolismo , Ácido gamma-Aminobutírico/análisis , Animales , Corteza Cerebral/ultraestructura , Dendritas/análisis , Histocitoquímica , Técnicas para Inmunoenzimas , Microscopía Electrónica , Neuronas/análisis , Neuronas/clasificación , Neuronas/ultraestructura , Plata , Coloración y EtiquetadoRESUMEN
Cholecystokinin octapeptide (CCK-8) in CNS has been shown to function as a neuropeptide with potent anti-opioid activity. It hinders opioid analgesia and facilitates opioid tolerance. The present study showed that electroacupuncture (EA) stimulation produced a marked increase of the CCK-8 immunoreactivity (ir) in the perfusate of the rat spinal cord. The increase of CCK-8-ir was most marked in response to EA of 100 Hz and 15 Hz, and less marked in response to EA of 2 Hz. Since CCK-8 has been shown to possess potent anti-opioid activity at the spinal level, blockade of the spinal CCK effect would be expected to potentiate EA-induced analgesia which is known to be opioid-mediated. Intrathecal (i.t.) administration of CCK-B antagonist L-365,260 per se did not affect tail flick latency (TFL) to any significant extent, yet it potentiated EA induced analgesia in a dose- and frequency-dependent manner. The potentiation was most marked at a dose range of 2.5-5.0 ng (i.t.) and at a frequency rank order of 100 Hz > 15 Hz > 2 Hz. The results suggest that an increased release of CCK-8 following EA may limit the effect of opioid peptides, and that the CCK-B receptor mediates the anti-opioid effect of CCK-8 in rat spinal cord.
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Analgesia por Acupuntura/métodos , Electroacupuntura , Neuropéptidos/metabolismo , Compuestos de Fenilurea , Sincalida/metabolismo , Médula Espinal/metabolismo , Animales , Benzodiazepinonas/administración & dosificación , Inyecciones Espinales , Masculino , Radioinmunoensayo , Ratas , Ratas Wistar , Receptores de Colecistoquinina/antagonistas & inhibidoresRESUMEN
The appearance of bicuculline-induced epileptiform discharges was studied in embryonic turtle cortex using extracellular recording techniques. Drug-induced discharges occurred at an early stage of cortical plate formation, suggesting that mechanisms for synchronizing neuronal discharges are functional at this stage. Discharges originated in the medial cortex and increased in amplitude and decreased in frequency with development. gamma-Aminobutyric acid (GABA)-containing neurons and functional GABA receptors are present in advance of excitatory synchronizing mechanisms and may have a non-synaptic role in corticogenesis.
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Bicuculina , Corteza Cerebral/fisiopatología , Epilepsia/inducido químicamente , Receptores de GABA-A/fisiología , Tortugas/fisiología , Animales , Corteza Cerebral/embriología , Epilepsia/embriología , Epilepsia/fisiopatología , Receptores de GABA-A/efectos de los fármacos , Tortugas/embriologíaRESUMEN
The whole-cell patch-clamp method was used to study the development of functional GABA receptors in cortical neurons dissociated from embryonic turtles. GABA elicited an increase in membrane conductance, even from cells obtained from the earliest stages of corticogenesis. The GABA-mediated conductance had a mean value 7.4 times greater than membrane 'leak' conductance and increased with developmental age. In all stages studied, the response inverted polarity at a value approximating ECl- and was blocked by applications of bicuculline, suggesting that it was mediated by GABAA receptors. GABA receptors are thus present and functional very early in corticogenesis, preceding electrogenesis, synaptogenesis, and full neuronal differentiation.
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Corteza Cerebral/fisiología , Neuronas/fisiología , Ácido gamma-Aminobutírico/fisiología , Animales , Bicuculina/farmacología , Corteza Cerebral/citología , Corteza Cerebral/embriología , Conductividad Eléctrica , Electrofisiología , Desarrollo Embrionario y Fetal , Tortugas/embriologíaRESUMEN
In a prospective study on latent autonomic dysfunction in the Chiari type I malformation, 15 patients were examined for degree of sinus arrhythmia in the supine and the sitting positions with a deep breathing test, and for pupil size and function with electronic pupillometry after sympathicomimetic eyedrop stimulation. The 5 patients with brainstem signs had a marked disturbance of sinus arrhythmia, which was more pronounced in the supine than in the sitting position. The clinical signs suggested a dysfunction of the reflex centers in the medulla rather than of the lower cranial nerves. The patients also tended to have more anisocoria than healthy controls, but no clear-cut pattern existed as to pupil dysfunction. Of the 4 patients with syringomyelia, however, the two with the most extensive cord cavitations had a Horner-like pupil reaction ipsilaterally to a marked hand wasting, indicating a sympathetic lesion at the T1 level of the cord.
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Malformación de Arnold-Chiari/complicaciones , Arritmia Sinusal/etiología , Adulto , Anciano , Anisocoria/diagnóstico , Anisocoria/etiología , Anisocoria/fisiopatología , Malformación de Arnold-Chiari/diagnóstico , Arritmia Sinusal/diagnóstico , Tronco Encefálico/fisiopatología , Diagnóstico Diferencial , Electrocardiografía , Femenino , Síndrome de Horner/diagnóstico , Humanos , Masculino , Persona de Mediana Edad , Soluciones Oftálmicas , Postura , Estudios Prospectivos , Pupila/efectos de los fármacos , Trastornos de la Pupila/diagnóstico , Trastornos de la Pupila/etiología , Trastornos de la Pupila/fisiopatología , Siringomielia/etiología , Siringomielia/fisiopatologíaRESUMEN
Pain perception threshold (PPT) in the head was assessed with a pressure algometer in 40 control individuals (24 females and 16 males). Threshold was assessed at 11 symmetrical points on each side. The average threshold for each side was calculated, and differences between two sides were evaluated with an asymmetry index. Subjects were studied in relation to age and sex, and any difference in the symmetrical perception of pain on the head was registered. The deltoid muscle was chosen as a reference point, since it is rarely a site of trigger points. The reproducibility of the method was satisfactory. A significantly positive correlation was found between PPT value of the head and the deltoid muscle. There was no tendency to rise or fall in PPT during the same "session", which consisted of 3 separate complete measurements. The mean of mean values showed that there was little asymmetry as for the thresholds pertaining to the head in the total material. Age and sex apparently played little role as for the outcome of the test. The assessment of PPT in the head might be a useful tool in the study of lateralization of pain in unilateral headache syndromes.
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Dominancia Cerebral/fisiología , Cabeza/inervación , Cuello/inervación , Nociceptores/fisiología , Dimensión del Dolor/instrumentación , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Umbral Sensorial/fisiología , Piel/inervaciónAsunto(s)
Enfermedades de los Nervios Craneales/clasificación , Cefalea/clasificación , Síndrome de Horner/clasificación , Hipohidrosis/clasificación , Adulto , Anciano , Blefaroptosis/clasificación , Blefaroptosis/diagnóstico , Blefaroptosis/fisiopatología , Enfermedades de los Nervios Craneales/diagnóstico , Enfermedades de los Nervios Craneales/fisiopatología , Nervios Craneales/fisiopatología , Diagnóstico Diferencial , Dominancia Cerebral/fisiología , Femenino , Traumatismos Cerrados de la Cabeza/complicaciones , Traumatismos Cerrados de la Cabeza/diagnóstico , Traumatismos Cerrados de la Cabeza/fisiopatología , Cefalea/diagnóstico , Cefalea/fisiopatología , Síndrome de Horner/diagnóstico , Síndrome de Horner/fisiopatología , Humanos , Hipohidrosis/diagnóstico , Hipohidrosis/fisiopatología , Masculino , Persona de Mediana Edad , Examen Neurológico , Oftalmoplejía/clasificación , Oftalmoplejía/diagnóstico , Oftalmoplejía/fisiopatología , Sudoración/fisiología , Nervio Trigémino/fisiopatologíaRESUMEN
Three patients with chronic paroxysmal hemicrania (CPH) (1 M, 2 F) and 9 healthy controls (8 M 1 F) were studied with transcranial Doppler (TCD) sonography. One patient who was studied during the spontaneous attacks hyperventilated markedly. Middle cerebral artery velocity (VMCA) was measured in the first attack, and anterior cerebral artery velocity (VACA) in the second attack, respectively. VMCA and VACA decreased bilaterally during attack. VMCA started to decrease at an early stage of the attack, i.e. prior to the major hyperventilation that was observed during the attack. VACA on the symptomatic side decreased less than that on the other side (P < 0.05). Cerebral vasomotor reactivity (VMR) was expressed as the percentage change in mean blood flow velocity as a function of end-tidal PCO2 (PETCO2) reduction induced by voluntary hyperventilation (delta V/delta PETCO2). In the 3 patients, a slightly lowered VMR was observed in the MCA and posterior cerebral artery on both sides, and in the ACA on the symptomatic side (P > 0.05) in comparison with controls. These observations may imply an abnormal vascular reactivity in CPH.
Asunto(s)
Trastornos Migrañosos/diagnóstico por imagen , Ultrasonografía Doppler Transcraneal , Adulto , Anciano , Circulación Cerebrovascular , Enfermedad Crónica , Femenino , Humanos , Masculino , Persona de Mediana Edad , Trastornos Migrañosos/fisiopatología , Recurrencia , Sistema Vasomotor/fisiopatologíaRESUMEN
Cluster headache and chronic paroxysmal hemicrania are assumed to be so closely related that they from a classification point of view have been grouped together under the superstructure: cluster headache syndrome. If this grouping will prove to stand the test of time, the pathogenesis of the two subgroups ought to be quite similar. CPH per se has two subgroups: those with and those without mechanical precipitation of attacks. If the aforementioned grouping of CPH is correct, then the CPH subdivision with mechanical precipitation of attacks should also be closely akin to cluster headache. Cluster headache, however, seems to lack the "nuchal" factor. It is felt that "midline structures", like the cavernous sinus, are of importance in cluster headache (cluster headache syndrome?) pathogenesis. In CPH with mechanical precipitation, attacks may be precipitated via "cervical volleys" a.m. Kerr, activating "midline" cavernous sinus structures, while the activation mechanisms in cluster headache is unknown.
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Cefalalgia Histamínica/fisiopatología , Seno Cavernoso/inervación , Nervios Craneales/fisiopatología , Ojo/inervación , Humanos , Presión Intraocular/fisiología , Mecanorreceptores/fisiopatología , Músculos del Cuello/inervaciónRESUMEN
The dorsal and medial telencephalon of reptiles consists of a simple trilaminar cortex. The turtle dorsal cortex has been identified as a favorable physiological preparation that may bear a phylogenetic relationship to mammalian neocortex. While anatomical studies have likened the reptilian medial cortical region to mammalian hippocampus, its physiological properties have not been explored. We therefore used intracellular and extracellular recording techniques to examine the cellular and synaptic physiology of turtle "hippocampal" or medial cortex. Turtle medial cortex contains two principal classes of neurons, pyramidal cells and stellate neurons. Recordings with Lucifer yellow CH (LY)-filled microelectrodes allowed us to correlate the physiological properties of medial cortical neurons with their cellular morphology. Pyramidal neurons were situated in a single cellular layer and had spiny apical dendrites extending into the molecular layer. These cells fired relatively long-duration action potentials (APs) and showed frequency adaptation to suprathreshold current pulse injections. Stellate cells were usually found in the subcellular and molecular layers and had aspiny dendrites. In contrast to pyramidal cells, they fired brief APs and displayed no frequency adaptation. A discrete population of cells in the dorsal portion of medial cortex (DMC) was capable of bursting endogenously or in response to synaptic activation. Bursts usually contained an underlying slow depolarization and often occurred at regular intervals. Intracellular LY injections confirmed that these cells were pyramidal in morphology. Electrical stimulation of afferent fibers revealed that pyramidal cells and stellate neurons differed in their synaptic responses. In ventral medial cortex (VMC), afferent stimulation evoked a multiphasic response in most pyramidal cells, whereas stellate cells were synaptically excited. Orthodromic activation of DMC bursting cells resulted in a powerful excitation--often a short burst--and subsequent inhibition. Stellate neurons in DMC also had a biphasic synaptic response consisting of both an early excitation and a late inhibition. Experiments using intracellular chloride (Cl-) injection or focal bicuculline application suggested that part of the inhibitory component of the pyramidal cell synaptic response was dependent on a gamma-aminobutyric acid (GABA)-mediated increase in Cl- conductance. These results correlated with our immunohistochemical studies that revealed the presence of GABAergic neurons in medial cortex.(ABSTRACT TRUNCATED AT 400 WORDS)
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Hipocampo/fisiología , Excitación Neurológica , Tortugas/fisiología , Animales , Mapeo Encefálico , Dendritas/fisiología , Electrólitos/metabolismo , Potenciales Evocados , Canales Iónicos/fisiología , Neuronas/clasificación , Neuronas/fisiología , Receptores de GABA-A/fisiología , Sinapsis/fisiología , Ácido gamma-Aminobutírico/fisiologíaRESUMEN
Four patients with SUNCT syndrome (Short-lasting, Unilateral, Neuralgiform headache attacks with Conjunctival injection and Tearing) were investigated with Doppler ultrasonography. Blood flow velocity (V) was measured in all intracranial arteries during both normocapnia and voluntary hyperventilation in 4 patients outside attacks (2 during remission; 2 during bout, but outside attacks) and in 8 healthy individuals. Vasomotor reactivity (VMR) was calculated on the basis of the formula of percentage change in V divided by the reduction in end-tidal PCO2 (PETCO2). Under the basal condition, the patients had a slightly, but non-significantly higher V in the middle cerebral artery (MCA) (P > 0.1) and lower V in the basilar artery (P > 0.05) than controls. During hyperventilation, a significant reduction in V was observed in the anterior and posterior cerebral arteries, at a level 1.5-2 SD above that in controls (P < 0.05), but a non-significant difference in VMR in comparison with controls. VMCA was continuously insonated during spontaneous (n = 8) and precipitated (n = 4) attacks in one particular patient on different days. Prior to attack, VMCA was significantly lower on the symptomatic side than on the non-symptomatic side (P < 0.014). VMCA decreased significantly during spontaneous attacks on both sides (P < 0.01) in comparison with the pre-attack stage, and returned to baseline before the cessation of attack. Similar findings were made during precipitated attacks. PETCO2, was rather constant throughout the entire attack study. Our data suggest that abnormal cerebral circulation may be part of the SUNCT syndrome. The vascular changes may have underlying mechanisms differing from those of the pain.
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Velocidad del Flujo Sanguíneo , Circulación Cerebrovascular , Cefalea/diagnóstico por imagen , Cefalea/fisiopatología , Neuralgia/fisiopatología , Ultrasonografía Doppler Transcraneal , Enfermedad Aguda , Humanos , Masculino , Persona de Mediana Edad , SíndromeRESUMEN
To test the influence of arterial O2 saturation (SaO2) on heart rate in cluster headache, changes in pulse rate induced by hyperoxia and hypoxia were monitored in 11 cluster headache patients (6 during cluster period, and 5 during remission). The results were compared with those obtained in 11 age and sex matched healthy individuals. The subjects were administered 5 min each of 100% O2 and 12% O2 in nitrogen in sequence. The aim of the latter procedure was to reduce SaO2 to approximately 80%. Pulse rate was recorded every minute from a finger pulse oximeter during the whole procedure. Cluster headache patients, in particular during the bout, had a slightly lower basal pulse rate than controls (P > 0.5, Student's t-test). This tendency was maintained throughout the test. Hyperoxia and hypoxia resulted in a marked, significant decrease and increase, respectively, in pulse rate from baseline values within each group. However, the difference between groups was not significant at any stage. Cluster headache patients therefore seem to have the same heart rate response to changes in SaO2 as healthy individuals. The marked heart rate changes which sometimes accompany cluster headache are unlikely to be caused by SaO2 changes.
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Cefalalgia Histamínica/sangre , Cefalalgia Histamínica/fisiopatología , Oxígeno/sangre , Pulso Arterial , Adulto , Arterias , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Transcranial Doppler ultrasound (TCD) investigations have been carried out in cluster headache patients (8 during remission and 6 during bout) and 14 healthy subjects, to assess cerebral vasomotor reactivity (VMR) to hypocapnia induced by voluntary hyperventilation. VMR was expressed as the relative change in blood flow velocity (V) (%) as a function of the reduction in end-tidal PCO2 (PETCO2) (kPa), i.e. V/P ETCO2. TCD with simultaneous PETCO2 monitoring, was also performed in 5 patients during spontaneous attacks. Prior to hyperventilation, there was bilaterally lower anterior cerebral artery velocity (VACA) during the bout than during remission (P < 0.05 on the symptomatic side), and also lower than in the controls. During remission, VACA was higher on the symptomatic side than on the other side (P < 0.05). ACA also showed a lower VMR during the bout than during remission, and it was also lower than in controls (bout vs. remission on the non-symptomatic side, P < 0.01; on the symptomatic side, P > 0.1). Approximately 30 minutes after the onset of attack, PETCO2 started to decrease gradually from 4.65 to 4.10 kPa in one patient with severe attack. The VACA decreased markedly and bilaterally already at an early stage of the attack, i.e. prior to the hyperventilation. Middle cerebral artery velocity tended to decrease 30 minutes after the onset of attack on the symptomatic side, and 50 minutes after onset on the non-symptomatic side. It is concluded that the vascular changes observed most likely are secondary phenomena during the cluster headache attack.
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Circulación Cerebrovascular , Cefalalgia Histamínica/diagnóstico por imagen , Hipocapnia/diagnóstico por imagen , Ultrasonografía Doppler Transcraneal , Adulto , Anciano , Cefalalgia Histamínica/fisiopatología , Femenino , Humanos , Hipocapnia/fisiopatología , Masculino , Persona de Mediana Edad , Sistema Vasomotor/fisiopatologíaRESUMEN
SUNCT is a headache syndrome characterized by short-lasting (usually 15-120 sec), unilateral head pain paroxysms localized in the peri-ocular area, accompanied by conjunctival injection, lacrimation, nasal stuffiness, rhinorrhea, and subclinical forehead sweating, all on the symptomatic side. A relative bradycardia seems to be an integral part of the paroxysm; a parasympathetic stimulation could theoretically be the causative factor for the bradycardia. In 3 SUNCT patients, vagal nerve function (E:I ratio) has been monitored outside and during pain paroxysms, while 3 other patients could be studied in the attack-free period only. E:I ratio is obtainable in the course of a maximally deep breath and represents the ratio of the longest R-R interval during a 5 sec long expiration to the shortest R-R interval during a 5 sec long expiration. The mean E:I ratio of SUNCT patients outside paroxysms was significantly higher than the mean E:I ratio in an aged-matched control group. The E:I ratio was, however, significantly decreased during paroxysms in comparison with ratios obtained outside the pain paroxysms. After 0.6 mg atropine administration s.c. to one of the patients in the symptomatic phase, the heart rate increased, and the relative bradycardia during headache paroxysm was diminished (but not completely abolished). The E:I ratio was lowered but it was still slightly larger outside than during attacks. The reason for the abrupt and seemingly clear attack-related decrement in E:I ratio together with the previously described relative bradycardia remains enigmatic, however the possibility of increased parasympathetic tone cannot be excluded.
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Arritmia Sinusal/fisiopatología , Cefalea/fisiopatología , Nervio Vago/fisiopatología , Anciano , Femenino , Frecuencia Cardíaca/fisiología , Humanos , Masculino , Persona de Mediana Edad , Neuralgia/fisiopatología , Respiración/fisiología , Síndrome , Lágrimas/metabolismo , Factores de TiempoRESUMEN
The forehead sweating function has been assessed in SUNCT syndrome--a short-lasting, unilateral, neuralgiform headache syndrome with autonomic phenomena on the symptomatic side (conjunctival injection, lacrimation, etc.). In the three patients (of a total of six) who could be studied during paroxysms, increased evaporation was present on the symptomatic side of the forehead compared to the non-symptomatic side during attacks or to the symptomatic side between attacks. Basal sweating was generally within control limits, so long as the attack frequency was not so high as to influence the interictal level. During attacks precipitated by eating chocolate or sour apple (in the case of one of the patients), forehead sweating was also increased on the symptomatic side. The forehead sweating responses to heating and pilocarpine were without any notable or systematic asymmetries. The forehead sweating pattern in SUNCT syndrome may differ from the patterns in unilateral headaches like cluster headache, on the one hand (in which there is generally an asymmetry during heating and pilocarpine tests), and chronic paroxysmal hemicrania (CPH) and cervicogenic headache, on the other (where there is no systematic increase during attacks.
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Cefalalgia Histamínica/clasificación , Cefalalgia Histamínica/fisiopatología , Frente , Sudoración , Anciano , Cacao , Ingestión de Alimentos , Frutas , Humanos , Masculino , Persona de Mediana Edad , Pilocarpina/farmacología , Sudoración/efectos de los fármacos , Sudoración/fisiología , SíndromeRESUMEN
To determine whether the carotid body plays a pathogenetic role in cluster headache, 20 cluster headache patients have been studied. Of these, 11 patients were in the interparoxysmal cluster phase, and 9 were in remission. Comparison was made with healthy subjects matched for sex, age, and smoking habits. Transient hypoxia was induced by inhalation of 1-8 breaths of 100% nitrogen (N2), until the arterial oxygen saturation (SaO2) decreased to around 80%. Changes in ventilation (tidal volume, inspiratory minute ventilation (VI), and end-tidal PCO2 (PETCO2)), were analyzed breath-by-breath. Under basal conditions, cluster headache patients had a slightly higher SaO2 and VI when compared to controls. PETCO2 was significantly lower (P < 0.05) during the cluster period as measured by Wilcoxon signed rank test for paired data, and during remission, according to the Student's paired t-test, in comparison with controls. After exposure to N2, no significant difference was found in the rate of reduction of SaO2 between any of the groups. A higher absolute increase in VI, but a relative (%) decrease in VI at moderate hypoxia were measured, the differences between patients and controls being on the border of the level of significance. Chemoreceptor sensitivity of the carotid body, expressed as the slope of a regression curve obtained by plotting the increase in VI against the reduction in SaO2, showed no statistical difference between the groups. The results do not support the hypothesis of a pathogenetic role for the carotid body in cluster headache.