RESUMEN
In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae.
Asunto(s)
Ritmo Circadiano , Microbioma Gastrointestinal/fisiología , Mariposas Nocturnas/microbiología , Animales , Bacterias/clasificación , Larva/microbiología , Mariposas Nocturnas/crecimiento & desarrollo , FilogeniaRESUMEN
To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After feeding on the diets with different pH values for 84 hr, fourth-instar caterpillars were randomly collected. Growth and various physiological traits were determined and 16S recombinant DNA sequencing was performed using the intestinal microflora of surviving larvae. Results showed that the mortality was 30% in DPB6, and 10% in DPB8, while no mortality was observed in control. The partial least squares discriminant analyses suggested that diets prepared with PB of different pH resulted in different food intake, amount of produced feces, weight gain, digestive enzyme activities, and antioxidant enzyme activities in larvae. Interestingly, both the highest weight gain and the lowest total antioxidant capacities were seen in control larvae. Results also showed that the larval gut microbiota community structure was significantly affected by dietary pH. Moreover, linear discriminant analysis effect size suggested that the family Acetobacteraceae in control, genus Prevotella in DPB8, and genus Lactococcus, family Flavobacteriaceae, family Mitochondria, and family Burkholderiaceae in DPB6 contributed to the diversity of the larval gut microbial community.
Asunto(s)
Alimentación Animal/análisis , Microbioma Gastrointestinal/efectos de los fármacos , Mariposas Nocturnas/crecimiento & desarrollo , Mariposas Nocturnas/microbiología , Animales , Dieta , Concentración de Iones de Hidrógeno , Larva/crecimiento & desarrollo , Larva/microbiologíaRESUMEN
Lymantria dispar asiatica is a globally distributed herbivorous pest. Avermectin is a highly effective, broad-spectrum insecticide. In this study, fourth instar L. dispar asiatica larvae were exposed to a LC30 dose of avermectin. The structure and function of larval gut microbial community was analyzed to examine how gut microbiota in L. dispar asiatica larvae responded to avermectin stress. Results showed that the structure and function of gut microbial community in L. dispar asiatica larvae were varied by avermectin stress. To be precise, more than half quantity of the observed Optical Taxonomic Units (OTUs) showed significantly different abundances under avermectin stress. Linear discriminant analysis effect size (LEfSe) suggested nine bacterial genera and 12 fungal genera contributed to the different gut microbial community structure in L. dispar asiatica larvae. Gut microbial function classification (PICRUSt and FUNGuild) suggested that three bacterial function categories and a fungal function guild were significantly increased, and two fungal function guilds were significantly decreased by avermectin stress. This study furthers our understanding of the physiology of L. dispar asiatica larvae under avermectin stress, and is an essential step towards future development of potential pesticide targets.
Asunto(s)
Insecticidas , Lepidópteros , Mariposas Nocturnas , Animales , Ivermectina/análogos & derivados , LarvaRESUMEN
This study investigated the distribution of Perfluorooctanesulfonate (PFOS) and Perfluorooctanoate (PFOA) in Fenhe River. A solid phase extraction (SPE) pretreatment method was used together with liquid chromatography tandem mass spectrometry (LC-MS/MS) to analyze the concentrations of PFOS and PFOA in Fenhe River, which were found to be in the ranges of 3.54-16.23 ng x L(-1) and 2.49-4.79 ng x L(-1) in the water phase, and in the ranges of 7.77-51.22 ng x g(-1) and 1.94-3.54 ng x g(-1) in the sediment, respectively. In water samples, the PFOS levels showed an increasing trend from the upstream to the downstream of the river, while the PFOA levels were similar among all the samples. In sediment samples, the increasing tendency of PFOS concentrations was not as obvious as in water samples, and the PFOA levels were again similar. Besides, the partitioning of PFOS between the water phase and the sediment was related to the content of organic carbon in the sediment. However, there was no significant correlation between PFOA partitioning and the organic carbon content.
Asunto(s)
Ácidos Alcanesulfónicos/análisis , Caprilatos/análisis , Fluorocarburos/análisis , Sedimentos Geológicos/química , Ríos/química , Contaminantes Químicos del Agua/análisis , China , Cromatografía Liquida , Espectrometría de Masas , Espectrometría de Masas en TándemRESUMEN
Estrogens and alkylphenols have received much attention because of its endocrine disrupting effects to aquatic ecosystem in recent years. The fate of these compounds in sediments which is a repository of many organic pollutants has an important significance on the study of behaviors of target compounds in the environment. It is difficult to separate trace estrogens from sediments with complex matrices. Alkali extraction, liquid-liquid extraction and LC-MS/MS were used to analyze estrogens, nonylphenol, 4-tert-octylphenol and bisphenol A in sediments based on their physicochemical properties. The results showed that recoveries of the seven target compounds were 61.3%-93.7%. The established pretreatment method can effectively remove pollutants that cannot dissolve in alkali solution or that cannot dissolve in both acid and alkali solution. It can widely be used to analyze sediments and soil samples because of its low cost and simple operation but with high recoveries and low detection limit.
Asunto(s)
Compuestos de Bencidrilo/análisis , Estrógenos/análisis , Sedimentos Geológicos/análisis , Fenoles/análisis , China , Monitoreo del Ambiente , Contaminantes Químicos del Agua/análisisRESUMEN
This paper investigated the contamination levels of heavy metal and polycyclic aromatic hydrocarbons (PAHs) in street dusts in different functional areas in urban Beijing. Results show that the mean concentrations of Cd, Hg, Cr, Cu, Ni, Pb and Zn in street dusts in Beijing are 710 ng/g, 307 ng/g, 85.0 microg/g, 78.3 microg/g, 41.1 microg/g, 69.6 microg/g and 248.5 microg/g, respectively, which are significantly lower than those in most cities around the world and Shenyang, Shanghai in China. The mean concentration of Sigma 16PAHs in street dusts in Beijing is 0.398 microg/g, which is also lower than those of Handan, Tianjin and Shanghai. Non-parametric Friedman test demonstrates significant differences of heavy metal contents on street dusts from different functional zones. Street dusts in residential area and parks have lower heavy metal and PAHs concentrations than the street dusts from areas of high traffic density. The concentrations of heavy metals follow the order Zn > Cr > Cu > Pb > Ni > Cd > Hg, which is consistent with the situation in other cities around the world. The geoaccumulation index analysis shows that street dust in urban Beijing is moderately polluted by Cd, Zn and Cu, little polluted by Cr and Pb and practically unpolluted by Ni. The contamination levels of Sigma 16PAHs on street dusts vary greatly in different functional zones with parks little polluted, residential areas moderately to strongly polluted and traffic related areas strongly polluted to extremely polluted. Mass loading of heavy metals and PAHs is largely associated with street dusts of size range < 300 microm. Therefore, the urban sweeping vehicles should update the dust sweeping devices to remove not only the fine particle but also the coarser particles.