Circular RNA Cdyl promotes abdominal aortic aneurysm formation by inducing M1 macrophage polarization and M1-type inflammation.

Macrophage polarization plays a crucial role in regulating abdominal aortic aneurysm (AAA) formation. Circular RNAs (circRNAs) are important regulators of macrophage polarization during the development of cardiovascular diseases. How-ever, the roles of circRNAs in regulating AAA formation through modulation of macrophage polarization remain unknown. In the present study, we compared circRNA microarray data under two distinct polarizing conditions (M1 and M2 macrophages) and identified an M1-enriched circRNA, circCdyl. Loss- and gain-of-function assay results demonstrated that circCdyl overexpression accelerated angiotensin II (Ang II)- and calcium chloride (CaCl2)-induced AAA formation by promoting M1 polarization and M1-type inflammation, while circCdyl deficiency showed the opposite effects. RNA pulldown, mass spectrometry analysis, and RNA immunoprecipitation (RIP) assays were conducted to elucidate the underlying mechanisms by which circCdyl regulates AAA formation and showed that circCdyl promotes vascular inflammation and M1 polarization by inhibiting interferon regulatory factor 4 (IRF4) entry into the nucleus, significantly inducing AAA formation. In addition, circCdyl was shown to act as a let-7c sponge, promoting C/EBP-δ expression in macrophages to induce M1 polarization. Our results indicate an important role for circCdyl-mediated macrophage polarization in AAA formation and provide a potent therapeutic target for AAA treatment.

Sumanene Monolayer of Pure Carbon: A Two-Dimensional Kagome-Analogy Lattice with Desirable Band Gap, Ultrahigh Carrier Mobility, and Strong Exciton Binding Energy.

The design and synthesis of novel two-dimensional (2D) materials that possess robust structural stability and unusual physical properties may open up enormous opportunities for device and engineering applications. Herein, a 2D sumanene lattice that can be regarded as a derivative of the conventional Kagome lattice is proposed. The tight-binding analysis demonstrates sumanene lattice contains two sets of Dirac cones and two sets of flat bands near the Fermi surface, distinctively different from the Kagome lattice. Using first-principles calculations, two possible routines for the realization of stable 2D sumanene monolayers (named α phase and ß phase) are theoretically suggested, and an α-sumanene monolayer can be experimentally synthesized with chemical vapor deposition using C21 H12 as a precursor. Small binding energies on Au(111) surface (e.g., -37.86 eV Å-2 for α phase) signify the possibility of their peel-off after growing on the noble metal substrate. Importantly, the GW plus Bethe-Salpeter equation calculations demonstrate both monolayers have moderate band gaps (1.94 eV for α) and ultrahigh carrier mobilities (3.4 × 104 cm2  V-1  s-1 for α). In particular, the α-sumanene monolayer possesses a strong exciton binding energy of 0.73 eV, suggesting potential applications in optics.

Photoredox-Catalyzed Synthesis of C-Benzoselenazolyl/Benzothiazolyl Glycosides from 2-Isocyanoaryl Selenoethers/Thioethers and Glycosyl Bromides.

Molecules containing heteroatoms, such as Se and S, play an indispensable role in the discovery and design of pharmaceuticals, whereas Se has been less studied. Here, we described a photoredox strategy to synthesize C-benzoselenazolyl (Bs) glycosides from 2-isocyanoaryl selenoethers and glycosyl bromides. This reaction was carried out under mild conditions with high efficiency. C-Benzothiazolyl (Bt) glycosides could also be synthesized from 2-isocyanoaryl thioethers using this strategy. This method can access novel seleno/thiosugars, which will benefit Se/S-containing drug discovery.

Highly selective fluorescence detection of L-selenium-methylselenocysteine in selenium-enriched Cardamine violifolia.

A feasible and practicable "off-on" type of fluorescence strategy for highly selective screening of L-selenium-methylselenocysteine (L-SeMC) in selenium-enriched Cardamine violifolia was developed using g-C3N4-MnO2 nanocomposites as fluorescent probes. The g-C3N4 nanosheets can emit blue fluorescence at 320 nm excitation wavelength with a fluorescence quantum yield of 28%. When MnO2 was deposited onto g-C3N4 nanosheets, the fluorescence of the g-C3N4 nanosheets was quenched due to fluorescence resonance energy transfer (FRET). After the addition of L-SeMC, MnO2 was reduced to Mn2+, which eliminated FRET and fluorescence was restored. Based on this, a quantitative method for the determination of L-SeMC was established. The fluorescence intensity of g-C3N4-MnO2 nanocomposites showed a good linear relationship with the concentration of L-SeMC in the range of 0-45 µg mL-1, the limit of detection (LOD, 3σ/K) was 8.25 ng mL-1 and the correlation coefficient was 0.9904. Common selenium compounds such as SeO2, Na2SeO3, SeMet and SeCys caused weak fluorescence intensity, which means that the developed method is highly selective to detect L-SeMC in a series of selenium compounds. Meanwhile, the technique was evaluated by spiking L-SeMC standards in C. violifolia extraction solutions and with 9 C. violifolia extraction specimens, receiving excellent accordance with results from the commercially available atomic fluorescence spectroscopy method.

Quality-driven deep cross-supervised learning network for semi-supervised medical image segmentation.

Semi-supervised medical image segmentation presents a compelling approach to streamline large-scale image analysis, alleviating annotation burdens while maintaining comparable performance. Despite recent strides in cross-supervised training paradigms, challenges persist in addressing sub-network disagreement and training efficiency and reliability. In response, our paper introduces a novel cross-supervised learning framework, Quality-driven Deep Cross-supervised Learning Network (QDC-Net). QDC-Net incorporates both an evidential sub-network and an vanilla sub-network, leveraging their complementary strengths to effectively handle disagreement. To enable the reliability and efficiency of semi-supervised training, we introduce a real-time quality estimation of the model's segmentation performance and propose a directional cross-training approach through the design of directional weights. We further design a truncated form of sample-wise loss weighting to mitigate the impact of inaccurate predictions and collapsed samples in semi-supervised training. Extensive experiments on LA and Pancreas-CT datasets demonstrate that QDC-Net surpasses other state-of-the-art methods in semi-supervised medical image segmentation. Code release is available at https://github.com/Medsemiseg.

Optogenetic Stimulation of the Cardiac Vagus Nerve to Promote Heart Regenerative Repair after Myocardial Infarction.

Background: It had been shown that selective cardiac vagal activation holds great potential for heart regeneration. Optogenetics has clinical translation potential as a novel means of modulating targeted neurons. This study aimed to investigate whether cardiac vagal activation via optogenetics could improve heart regenerative repair after myocardial infarction (MI) and to identify the underlying mechanism. Methods: We used an adeno-associated virus (AAV) as the vector to deliver ChR2, a light-sensitive protein, to the left nodose ganglion (LNG). To assess the effects of the cardiac vagus nerve on cardiomyocyte (CM) proliferation and myocardial regeneration in vivo, the light-emitting diode illumination (470 nm) was applied for optogenetic stimulation to perform the gain-of-function experiment and the vagotomy was used as a loss-of-function assay. Finally, sequencing data and molecular biology experiments were analyzed to determine the possible mechanisms by which the cardiac vagus nerve affects myocardial regenerative repair after MI. Results: Absence of cardiac surface vagus nerve after MI was more common in adult hearts with low proliferative capacity, causing a poor prognosis. Gain- and loss-of-function experiments further demonstrated that optogenetic stimulation of the cardiac vagus nerve positively regulated cardiomyocyte (CM) proliferation and myocardial regeneration in vivo. More importantly, optogenetic stimulation attenuated ventricular remodeling and improved cardiac function after MI. Further analysis of sequencing results and flow cytometry revealed that cardiac vagal stimulation activated the IL-10/STAT3 pathway and promoted the polarization of cardiac macrophages to the M2 type, resulting in beneficial cardiac regenerative repair after MI. Conclusions: Targeting the cardiac vagus nerve by optogenetic stimulation induced macrophage M2 polarization by activating the IL-10/STAT3 signaling pathway, which obviously optimized the regenerative microenvironment and then improved cardiac function after MI.

Photoredox-catalyzed C-heteroaryl glycosylation of biphenyl isocyanides with glycosyl bromides.

8,9-Dimethoxyphenanthridine derivatives, as potential antitumor drugs, need modification to improve their biocompatibility and water solubility. Reported here is a strategy to access C-heteroaryl glycosides by photoredox catalysis. C6-glycosylated phenanthridine derivatives are synthesized from biphenyl isocyanides and glycosyl bromides. The reaction conditions are mild and widely applicable, with anomeric α selectivity and good functional group tolerance.

Porous carbon-based metal-free monolayers towards highly stable and flexible wearable thermoelectrics and microelectronics.

In the search for high mechanical strength and flexibility, ultrahigh semiconducting speed is crucial for the next generation of microelectronic and wearable electronics. Herein, we propose two 2D graphene-like macrocyclic complex carbon-based monolayers, namely g-MC-A and g-MC-B. Both monolayers are dynamically stable according to phonon dispersion and ab initio molecular dynamics simulations. The yield stress of these two layers reaches half that of graphene, revealing remarkably high mechanical strength. Besides, both monolayers are semiconductors. The electron mobility of g-MC-A is high: up to 104 cm2 V-1 s-1, comparable to black phosphorene. Furthermore, these two monolayers exhibit excellent inherent conductivity with anisotropic characteristics. Interestingly, an extra valley is observed near the conduction band edge for both layers, further simulation predicted both metal-free monolayers will exhibit ZT > 1, implying high thermoelectric performance. Therefore, these two C-based metal-free layers have promising applications in mechanical enhancement, microelectronics, wearable electronics and thermoelectric devices.

Stability and superconductivity of freestanding two-dimensional transition metal boridene: M4/3B2.

The small atomic mass of boron indicates strong electron-phonon coupling (EPC), so it may have a brilliant performance in superconductivity. Recently, a new 2D boride sheet with ordered metal vacancies and surface terminals (Mo4/3B2-x) was realized in experiments (Zhouet al2021Science373801). Here, the 2D monolayer freestanding Mo4/3B2is evidenced to be thermodynamically stable. Through electronic structure, phonon spectrum and EPC, monolayer Mo4/3B2is found to be an intrinsic phonon-mediated superconductor. The superconducting transition temperature (Tc) is determined to be 4.06 K by the McMillian-Allen-Dynes formula. Remarkably, theTcof monolayer Mo4/3B2can be increased to 6.78 K with an appropriate biaxial tensile strain (+5%). Moreover, we predict that other transition metal replacing Mo atoms is also stable and retaining the superconductivity. Such as monolayer W4/3B2is also a superconductor with theTcof 2.37 K. Our research results enrich the database of 2D monolayer superconductors and boron-related formed materials science.