Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Más filtros

Banco de datos
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Br Poult Sci ; 57(4): 494-500, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27166917

RESUMEN

Forty-two enrofloxacin-resistant Escherichia coli strains isolated from eggs and first-week mortality associated with yolk sac infection of two vertically integrated poultry companies of Central Mexico in 1997 and 2005 were characterised. E. coli resistance to 19 antibiotics was determined, as well as the minimum inhibitory concentrations (broth dilution) for ciprofloxacin. The presence of gyrA,B, parC,E chromosomal point mutations, qnrA,B,S plasmid genes and the aminoglycoside acetyltransferase aac(6')-Ib-cr were determined by PCR and sequencing. Resistance to ampicillin (95%), piperacillin (95%), gatifloxacin (95%), levofloxacin (95%), ampicillin/sulbactam (90%), cefazolin (85%), trimethoprim/sulfamethoxazole (80%), amoxicillin/clavulanic acid (80%), aztreonam (80%), cefepime (80%), cefotaxime (80%), ceftazidime (80%), ceftriaxone (80%) and cefoxitin (75%) was high in the 2005 strains and 19 (95%) strains were resistant to 7 or more antimicrobials. The strains from 1997 expressed high rates of resistance only to the fluoroquinolones and 4 strains (18%) expressed resistance to 7 or more antimicrobials. All strains had a gyrA mutation (Ser83Leu) and a parC mutation (Ser80Ile or Ser80Arg) and 41 (97.6%) strains had a second gyrA mutation (Asp87Asn, Asp87Tyr or Asp87Gly). Only two (4.7%) strains had a parE mutation (Ser458Ala). A total of 10 strains were positive for the aac(6')-Ib wild-type gene, 6 strains for the aac(6')-Ib-cr variant and 6 strains possessed both the wild type and the variant. No gyrB mutations or qnrA,B,S genes were detected. This is the first report in Latin America of chromosomal and plasmid quinolone resistance genes in E. coli strains recovered from poultry.


Asunto(s)
Antibacterianos/farmacología , Farmacorresistencia Bacteriana , Infecciones por Escherichia coli/veterinaria , Fluoroquinolonas/farmacología , Enfermedades de las Aves de Corral/microbiología , Animales , Escherichia coli/efectos de los fármacos , Infecciones por Escherichia coli/microbiología , México , Pruebas de Sensibilidad Microbiana/veterinaria , Óvulo/microbiología , Aves de Corral
2.
Int J Antimicrob Agents ; 58(5): 106426, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34419579

RESUMEN

The worldwide spread of carbapenem- and polymyxin-resistant Enterobacterales represents an urgent public-health threat. However, for most countries in the Americas, the available data are limited, although Latin America has been suggested as a silent spreading reservoir for isolates carrying plasmid-mediated polymyxin resistance mechanisms. This work provides an overall update on polymyxin and polymyxin resistance and focuses on uses, availability and susceptibility testing. Moreover, a comprehensive review of the current polymyxin resistance epidemiology in the Americas is provided. We found that reports in the English and Spanish literature show widespread carbapenemase-producing and colistin-resistant Klebsiella pneumoniae in the Americas determined by the clonal expansion of the pandemic clone ST258 and mgrB-mediated colistin resistance. In addition, widespread IncI2 and IncX4 plasmids carrying mcr-1 in Escherichia coli come mainly from human sources; however, plasmid-mediated colistin resistance in the Americas is underreported in the veterinary sector. These findings demonstrate the urgent need for the implementation of polymyxin resistance surveillance in Enterobacterales as well as appropriate regulatory measures for antimicrobial use in veterinary medicine.


Asunto(s)
Farmacorresistencia Bacteriana/genética , Escherichia coli/efectos de los fármacos , Escherichia coli/genética , Klebsiella pneumoniae/efectos de los fármacos , Klebsiella pneumoniae/genética , Polimixinas/farmacología , Animales , Antibacterianos/farmacología , Proteínas Bacterianas/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Humanos , Klebsiella pneumoniae/metabolismo , Proteínas de la Membrana/genética , Pruebas de Sensibilidad Microbiana , América del Norte , Plásmidos/genética , América del Sur , beta-Lactamasas/genética
3.
Antimicrob Agents Chemother ; 52(8): 2943-6, 2008 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-18490501

RESUMEN

During 2003, 40 carbapenem-resistant Pseudomonas aeruginosa clinical isolates collected in a Mexican tertiary-care hospital were screened for metallo-beta-lactamase production. Thirteen isolates produced IMP-15, and 12 had a single pulsed-field gel electrophoresis pattern. The bla(IMP-15) gene cassette was inserted in a plasmid-borne integron with a unique array of gene cassettes and was named In95.


Asunto(s)
Proteínas Bacterianas/genética , Integrones/genética , Pseudomonas aeruginosa/genética , beta-Lactamasas/genética , Infección Hospitalaria/microbiología , Electroforesis en Gel de Campo Pulsado , Humanos , México , Modelos Genéticos , Datos de Secuencia Molecular , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/enzimología , Pseudomonas aeruginosa/aislamiento & purificación
4.
Genome Announc ; 4(4)2016 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-27389261

RESUMEN

A clinical isolate of extended-spectrum-ß-lactamase-producing Klebsiella quasipneumoniae subsp. similipneumoniae 06-219 with hypermucoviscosity phenotypes obtained from a urine culture of an adult patient was used for whole-genome sequencing. Here, we report the draft genome sequences of this strain, consisting of 53 contigs with an ~5.6-Mb genome size and an average G+C content of 57.36%. The annotation revealed 6,622 coding DNA sequences and 77 tRNA genes.

5.
Arch Med Res ; 28(2): 285-7, 1997.
Artículo en Inglés | MEDLINE | ID: mdl-9204623

RESUMEN

With the simultaneous use of an isoelectric focusing gel (IEF) and a nitrocefin/cefotaxime bioassay, it is possible to identify the Extended-Spectrum beta-lactamases (ESBL) with precision. A mixture of soft agar and susceptible bacterial cells are layered over the gel following overnight incubation and areas of cell growth are detected where the antibiotic has been hydrolyzed by specific enzymes. This innovative method improves sensitivity and specificity for the identification of ESBLs in those enterobacteria strains producing more than one beta-lactamase and are resistant to third generation cephalosporines.


Asunto(s)
Proteínas Bacterianas/análisis , Bioensayo , Enterobacteriaceae/enzimología , Focalización Isoeléctrica , Pruebas de Sensibilidad Microbiana , Resistencia betalactámica , beta-Lactamasas/análisis , Proteínas Bacterianas/metabolismo , Cefotaxima/metabolismo , Cefalosporinas/metabolismo , Escherichia coli/enzimología , Klebsiella pneumoniae/enzimología , Sensibilidad y Especificidad , beta-Lactamasas/metabolismo
6.
Arch Med Res ; 28(2): 195-203, 1997.
Artículo en Inglés | MEDLINE | ID: mdl-9204608

RESUMEN

In this review article, we make suggestions on how to approach the increasing problem worldwide of bacterial acute respiratory infections resistant to antibiotics. After a brief description of the main mechanisms of bacterial resistance, i.e., enzymatic inactivation by beta-lactamases, reduction in the permeability of the outer membrane and the development of PBPs that have decreased affinity for the antibiotic, we analyze documented experiences on the response to different groups of antibiotics (beta- lactam antibiotics, cephalosporins, carbapenems and quinolones), of the most commonly isolated bacteria from invasive respiratory infections (Haemophilus influenzae, Streptococcus pneumoniae and Moraxela (Branhamella) catarrhalis. Antimicrobial agent susceptibility in vivo and in vitro testing and the correlation of their results provide the basic information for the adoption of adequate policies and strategies for better use of antibiotics in bacterial respiratory infections; proper surveillance would allow to make intelligent changes in such a policy. Standardized recommendations for clinical practice on the use of antibiotics could be misleading, iatrogenic, and could complicate the resistance problem. To prevent and control the rise and spread of bacterial resistance, an interdisciplinary approach is needed.


Asunto(s)
Infecciones Bacterianas/tratamiento farmacológico , Farmacorresistencia Microbiana , Infecciones del Sistema Respiratorio/tratamiento farmacológico , Enfermedad Aguda , Adulto , Anciano , Antibacterianos/administración & dosificación , Antibacterianos/clasificación , Antibacterianos/farmacología , Infecciones Bacterianas/epidemiología , Niño , Ensayos Clínicos como Asunto , Resistencia a Múltiples Medicamentos , Femenino , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Infecciones del Sistema Respiratorio/epidemiología , Infecciones del Sistema Respiratorio/microbiología
7.
New Microbes New Infect ; 2(6): 173-4, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25566396

RESUMEN

A collection of 15 carbapenem-resistance Acinetobacter baumannii clinical isolates was analysed on two tertiary hospitals in Mexico. The OXA-51 was identified in all isolates, followed by OXA-239 and OXA-58; OXA-239 is described as a new OXA-23-like allele. These carbapenemases were identified on four clonal groups, distributed between two neighbouring hospitals. Acinetobacter baumannii is poorly studied in Mexico; this situation urges the implementation of strategies to prevent its dissemination.

9.
J Chemother ; 22(3): 160-4, 2010 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-20566419

RESUMEN

The production of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriaceae is the most prevalent resistance mechanism to third-generation cephalosporins. The aim of this study was to identify the ESBLs produced in Escherichia coli and Klebsiella pneumoniae clinical isolates from two hospitals of the Colombian Caribbean Region. A total of 30 clinical isolates of K. pneumoniae (21) and E. coli (9) ESBL-producers were collected in two hospitals from January, 2001 to June, 2003. Isoelectric point values were indicative of SHV-, and CTX-M-type beta-lactamases. PCR amplification and sequencing of SHV genes revealed that SHV-12 was the most prevalent ESBL followed by SHV-5, SHV-2a, the novel SHV-86 and CTX-M-12. There was a geographic distribution of two particular PFGE subtypes in these two distant hospitals. Clonal and horizontal dissemination of resistance was observed.


Asunto(s)
Escherichia coli/enzimología , Klebsiella pneumoniae/enzimología , beta-Lactamasas/genética , Región del Caribe , Farmacorresistencia Bacteriana Múltiple , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/microbiología , Hospitales , Humanos , Infecciones por Klebsiella/microbiología , Klebsiella pneumoniae/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Reacción en Cadena de la Polimerasa , beta-Lactamasas/metabolismo
11.
J Chemother ; 20(5): 586-92, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19028621

RESUMEN

Previous outbreaks caused by Serratia marcescens have been associated with contaminated medical equipment, intravenous fluids and inadequate hygiene. We carried out the molecular characterization of an outbreak produced by a cephalosporin-resistant S. marscescens that occurred in a Mexican hospital in August 1999. The lethality of this outbreak was 26%. Positive isolates were collected from 20 patients, one medical staff and three chlorhexidine disinfectant solutions. Results of PFGE, beta-lactamase patterns, sequencing of PCR amplifications, plasmid profiles, and mating experiments showed that the outbreak occurred by the dissemination of a S. marcescens SHV-5 producing strain. The adequate enforcement of procedures under the supervision of an infection control resulted in the abrupt end of the outbreak.


Asunto(s)
Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Infecciones por Serratia/epidemiología , Infecciones por Serratia/microbiología , beta-Lactamasas/biosíntesis , Southern Blotting , Infección Hospitalaria/genética , ADN Bacteriano , Farmacorresistencia Microbiana , Humanos , México , Reacción en Cadena de la Polimerasa , Infecciones por Serratia/genética , Serratia marcescens/genética , Serratia marcescens/aislamiento & purificación
12.
Rev Med Chir Soc Med Nat Iasi ; 105(1): 133-5, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-12092140

RESUMEN

OBJECTIVES: To characterize the OMP profile and to study the possible interference with other resistance determinants. MATERIAL AND METHODS: 16 non-typhoidic Salmonella strains, isolated in 1999 from stools of pediatric patients, were selected according to their resistance phenotype: resistance to Ampicillin (AMP), Amoxycillin/Clavulanic Acid (AMC), and third generation cephalosporins: Ceftriaxone (CRO) and Ceftazidime (CAZ). Identification and sensitivity testing were done by DADE MicroScan System. beta-Lactamases were characterized by isoelectric focusing (IEF) with preformed minigelss. OMPs were studied on membrane preparations on SDS-urea gels. RESULTS: Isoelectric points: Majority of the isolates had the association 5.4 +/- 7.6 (3/16) or 5.4 + 8.2 (6/16). The second beta-lactamase has the capacity to hydrolyse CAZ. We found three different patterns of OMPs: I: 30, 29 and 25 kDa; II: 30, 26 and 24 kDa; III: 30 and 25 kDa. We couldn't find any correlation between the OMPs profile and resistance phenotype, showing that the beta-lactamase production is the only resistant determinant. CONCLUSIONS: The study shows the high frequency of extended-spectrum beta-lactamases (ES beta LA) amongst the non-typhoidic Salmonella; association of TEM type and ES beta LA enzymes diminishes considerably the therapeutic resources: inhibitor associated combinations are non effective. In non-typhoidic Salmonella, porins seems to have no influence in co-modulation of resistance.


Asunto(s)
Proteínas de la Membrana Bacteriana Externa/fisiología , Farmacorresistencia Bacteriana/genética , Infecciones por Salmonella/microbiología , Salmonella/genética , beta-Lactamasas/biosíntesis , Antibacterianos/farmacología , Proteínas de la Membrana Bacteriana Externa/genética , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Penicilinas/farmacología , Rumanía/epidemiología , Salmonella/efectos de los fármacos , Salmonella/enzimología , Infecciones por Salmonella/epidemiología , beta-Lactamas
14.
J Clin Microbiol ; 42(8): 3877-80, 2004 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-15297554

RESUMEN

Between 1997 and 2000 a single multidrug-susceptible methicillin-resistant Staphylococcus aureus clone, M (sequence type 30 [ST30]-staphylococcal cassette chromosome mec [SCCmec] type IV), was present in a pediatric hospital in Mexico City, Mexico. In 2001 the international multidrug-resistant New York-Japan clone (ST5-SCCmec type II) was introduced into the hospital, completely replacing clone M by 2002.


Asunto(s)
Resistencia a la Meticilina/genética , Infecciones Estafilocócicas/epidemiología , Infecciones Estafilocócicas/prevención & control , Staphylococcus aureus/aislamiento & purificación , Niño , Electroforesis en Gel de Campo Pulsado , Hospitales Pediátricos , Humanos , México/epidemiología , Filogenia , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus aureus/clasificación , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/genética , Virulencia/genética
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA