RESUMEN
The visible and Mössbauer spectra of [Fe(II)(Por)L2] and [Fe(II)(Por)L(CO)] complexes (where Por = protoporphyrin IX (PPIX) or tetra(p-sulfophenyl)porphyrin (TPPS) and L = an aliphatic or aromatic nitrogenous base) are reported and discussed. The results are compared to those of previously reported [Fe(II)(Por)L(CO)] complexes (where Por = PPIX, TPPS, PMXPP, TPP, OMTBP and OEP; L = a nitrogenous aromatic ligand) and HbCO (where Hb = haemoglobin) and MyCO (where My = myoglobin). A new approach, to extracting information from the Mössbauer parameters has been developed by plotting those of the [Fe(II)(Por)L2] complexes against those of [Fe(II)(Por)L(CO)] complexes for the same ligands, has yielded a series of trend lines that show a significant dependence on both the nature of the porphyrin and also of the nitrogenous ligand. Different trend lines were found for aromatic nitrogenous ligands to aliphatic nitrogenous ligands showing that the porphyrins could donate different amounts of charge to the Fe(II) cations as the L ligand changed, and hence, they display electron sink properties. From the plots, it was shown that haemoglobin and myoglobin both bind CO very strongly compared to the model complexes studied herein. Using the reported structural and Mössbauer data for the [Fe(II)(Por)L2] and [Fe(II)(Por)L(CO)] complexes, it proved possible and instructive to plot the Mössbauer parameters against a number of the bond lengths around the Fe(II) cations. The interpretation of the resulting trend lines both supported and facilitated the extension of our findings enabling further understanding of the geometry of the bonding in CO haemoglobin and CO myoglobin.
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Mioglobina , Porfirinas , Compuestos Ferrosos , Hemoglobinas , Ligandos , Porfirinas/química , Monóxido de Carbono/químicaRESUMEN
Studies are reported on the formation of low-spin six-coordinate [Fe(PPIX)L2] complexes from iron(II) protoporphyrin where L is one of a series of nitrogenous ligands (aliphatic, aromatic or heterocyclic). The bonding constants have been determined by titration of the metal complex with these ligands and are compared in relation to previous studies. The adduct formation was monitored utilising optical spectroscopy. In addition, MÓ§ssbauer spectroscopic experiments were conducted to monitor the electronic environment around the central iron atom in these complexes. The two complementary spectroscopic methods indicated that all nitrogen ligands formed low-spin octahedral complexes. The magnitude of the overall binding constants (ß2 values) are discussed and related to (a) the pKa values of the free ligands and (b) the Mössbauer parameter ΔEQ, which represents the quadrupole splitting of the haem iron. The ß2 and ΔEQ values are also discussed in terms of the structure of the ligand. Cooperative binding was observed for nearly all the ligands with Hill coefficients close to 2 for iron(II) protoporphyrin; one of these ligands displayed a much greater affinity than any we previously studied, and this was a direct consequence of the structure of the ligand. Overall conclusions on these and previous studies are drawn in terms of aliphatic ligands versus aromatic ring structures and the absence or presence of sterically hindered nitrogen atoms. The implications of the work for the greater understanding of haem proteins in general and in particular how the nitrogenous ligand binding results are relevant to and aid the understanding of the binding of inhibitor molecules to the cytochrome P450 mono-oxygenases (for therapeutic purposes) are also discussed. Changes in the electronic absorption spectra of five-coordinate [Fe(II)(PPIX)(2-MeIm)] that occurred as the temperature was lowered from room temperature to 78° K.
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Hierro , Nitrógeno , Compuestos Ferrosos/química , Hemo , Concentración de Iones de Hidrógeno , Hierro/química , Ligandos , ProtoporfirinasRESUMEN
Purification of compounds is a necessary aspect of chemical synthesis. Developing an efficient purification method is time-consuming. A method that quickly calculates preparative gradients from analytical scouting runs is described. A solvent composition that provides the desired retention of a model compound is used to calibrate the analytical scouting run to determine an apparent gradient delay. This delay is applied to the retention times of compounds run with the same scouting gradient to yield a solvent composition for preparative purification.
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Productos Biológicos/aislamiento & purificación , Compuestos Orgánicos/aislamiento & purificación , Cromatografía Liquida/métodos , Interacciones Hidrofóbicas e Hidrofílicas , Modelos Químicos , Proteínas/aislamiento & purificación , Dióxido de Silicio/química , Solventes/química , Factores de TiempoRESUMEN
OBJECTIVE: Tumor necrosis factor (TNF)-alpha plays a role in the inflammatory process in Crohn disease, a disease with an apparent polygenic basis. We investigated whether polymorphisms in multiple genes involved in the lipopolysaccharide-TNF inflammatory pathway are independently associated with Crohn disease in the Jewish Ashkenazi population. Polymorphisms in CD14, Toll-like receptor 4 (TLR4), and TNF-alpha were studied. In addition, we investigated polymorphisms in the TNF-alpha converting enzyme (TACE) gene, which to date has not been studied for an association with Crohn disease. PATIENTS AND METHODS: To examine whether TLR4 Asp299Gly, CD14-260C/T, TNF-1031T/C, TNF-863C/A, TNF-857C/T, TACE-172C/T, and TACE-154C/A polymorphisms are associated with Crohn disease in the Ashkenazi Jewish population, we analyzed families with at least 1 child with Crohn disease for association with these mutations using a family-based association test (transmission disequilibrium test) for analysis. RESULTS: The allelic frequency in the patient population of TLR4 G allele was 8.0%, CD14 T allele was 51.3%, TNF-1031C was 18.8%, TNF-863A was 14.2%, TNF-857T was 25.2%, TACE172T was 20.7%, and TACE154A was 24.5%. The transmission disequilibrium test transmitted:untransmitted (T:U) result for TLR4G was T:U = 32:20, for CD14T was T:U = 103:88, for TNF-1031C was T:U = 48:56, for TNF-863A was T:U = 39:42, for TNF-857T was T:U = 63:62, for TACE-172C/T was T:U = 48:59, and for TACE-154C/A was T:U = 52:55. No statistically significant associations were observed. CONCLUSIONS: The transmission disequilibrium test did not demonstrate preferential transmission of these variants in Jewish Ashkenazi patients with Crohn disease. These results suggest that these polymorphisms in the TNF/lipopolysaccharide pathway play little or no role in susceptibility to Crohn disease in the Jewish Ashkenazi population.
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Enfermedad de Crohn/genética , Judíos/genética , Desequilibrio de Ligamiento/genética , Polimorfismo Genético , Factor de Necrosis Tumoral alfa/genética , Adolescente , Edad de Inicio , Femenino , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Humanos , Receptores de Lipopolisacáridos/genética , Lipopolisacáridos , Masculino , Receptor Toll-Like 4/genéticaRESUMEN
Herein, we describe three advanced techniques for cathodoluminescence (CL) spectroscopy that have recently been developed in our laboratories. The first is a new method to accurately determine the CL-efficiency of thin layers of phosphor powders. When a wide band phosphor with a band gap (Eg > 5 eV) is bombarded with electrons, charging of the phosphor particles will occur, which eventually leads to erroneous results in the determination of the luminous efficacy. To overcome this problem of charging, a comparison method has been developed, which enables accurate measurement of the current density of the electron beam. The study of CL from phosphor specimens in a scanning electron microscope (SEM) is the second subject to be treated. A detailed description of a measuring method to determine the overall decay time of single phosphor crystals in a SEM without beam blanking is presented. The third technique is based on the unique combination of microscopy and spectrometry in the transmission electron microscope (TEM) of Brunel University London (UK). This combination enables the recording of CL-spectra of nanometre-sized specimens and determining spatial variations in CL emission across individual particles by superimposing the scanning TEM and CL-images.
RESUMEN
Herein we describe the synthesis and luminescence of nanosized (Lu1-y-xGdx)2O2S:Tby and (Lu1-y-xGdx)2O3:Tby phosphors with y = 0.1 mol% Tb3+ and y = 2 mol% Tb3+ and x ranging between 0 and 1. The concentration of Gd3+ (x) was varied in steps of 0.1 (molar ratio Gd3+). The samples at 0.1 < x < 0.7 contained a mixture of (Lu1-xGdx)2O3:Tb3+ and (Lu1-xGdx)2O2S:Tb3+, while the samples at x = 0 contained only Lu2O3:Tb3+. At 0.1 < x < 0.7 Lu2O2S:Tb3+ and Gd2O2S:Tb3+ did not form a solid solution, but rather crystallised into two slightly different hexagonal structures. This behaviour has been explained in terms of segregation of Lu and Gd between the oxide and oxysulfide phases: the oxide phase is more Lu-rich whereas the second oxysulfide phase is more Gd-rich. The photoluminescence spectra of the phosphors with 0.1 mol% Tb3+ showed a modest colour change of the fluorescence light from cyan to green when x was increased from 0 to 1, whereas the samples of the series with 2 mol% Tb3+ yielded essentially green light. From this analysis it was concluded that the colour change of (Lu1-xGdx)2O2S:0.1%Tb3+ is caused by increasing energy transfer of the 5D3-level of Tb3+ to the charge transfer band of (Lu1-xGdx)2O2S:Tb3+ upon increasing x. Since the samples with 100% Lu consisted of pure cubic Lu2O3:Tb3+, we had the opportunity to also study the symmetry-related PL of this compound. From this study we concluded that the C2-C3i doublet of the Tb3+ 5D4 â 7F5 transition behaves in the same way as the corresponding doublet in cubic Y2O3:Tb3+.
RESUMEN
The black pigment of Porphyromonas gingivalis is composed of the mu-oxo bishaem complex of Fe(III) protoporphyrin IX (mu-oxo oligomer, dimeric haem), namely [Fe(III)PPIX]2O. P. gingivalis W50 and Rgp (Arg-gingipain)- and Kgp (Lys-gingipain)-deficient mutants K1A, D7, E8 and W501 [Aduse-Opoku, Davies, Gallagher, Hashim, Evans, Rangarajan, Slaney and Curtis (2000) Microbiology 146, 1933-1940] were grown on horse blood/agar for 14 days and examined for the production of mu-oxo bishaem. Mu-oxo Bishaem was detected by UV-visible, Mössbauer and Raman spectroscopies in wild-type W50 and in the black-pigmented RgpA- and RgpB-deficient mutants (W501 and D7 respectively), whereas no haem species were detected in the straw-coloured colonies of Kgp-deficient strain K1A. The dark brown pigment of the double RgpA/RgpB knockout mutant (E8) was not composed of mu-oxo bishaem, but of a high-spin monomeric Fe(III) protoporphyrin IX species (possibly a haem-albumin complex). In vitro incubation of oxyhaemoglobin with cells of the W50 strain and the RgpA- and RgpB-deficient mutants (W501 and D7) resulted in the formation of mu-oxo bishaem via methaemoglobin as an intermediate. Although the Kgp-deficient strain K1A converted oxyhaemoglobin into methaemoglobin, this was not further degraded into mu-oxo bishaem. The double RgpA/RgpB knockout was also not capable of producing mu-oxo bishaem from oxyhaemoglobin, but instead generated a haemoglobin haemichrome. Inhibition of Arg-X protease activity of W50, W501, D7 and K1A with leupeptin, under conditions where Lys-X protease activity was unaffected, prevented the production of mu-oxo bishaem from oxyhaemoglobin, but resulted in the formation of a haemoglobin haemichrome. These results show that one or both of RgpA and RgpB gingipains, in addition to the lysine-specific gingipain, is necessary for the production of mu-oxo bishaem from haemoglobin by whole cells of P. gingivalis.
Asunto(s)
Cisteína Endopeptidasas/metabolismo , Hemaglutininas/metabolismo , Oxihemoglobinas/metabolismo , Pigmentos Biológicos/química , Pigmentos Biológicos/metabolismo , Porphyromonas gingivalis/enzimología , Protoporfirinas/metabolismo , Adhesinas Bacterianas , Agar , Animales , Cisteína Endopeptidasas/deficiencia , Cisteína Endopeptidasas/genética , Cisteína-Endopeptidasas Gingipaínas , Hemaglutininas/genética , Caballos/sangre , Leupeptinas/metabolismo , Pigmentos Biológicos/análisis , Porphyromonas gingivalis/química , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/crecimiento & desarrollo , Protoporfirinas/análisis , Protoporfirinas/química , Espectrofotometría Ultravioleta , Espectroscopía de Mossbauer , Espectrometría Raman , Factores de TiempoRESUMEN
Cathodoluminescence (CL) studies are reported on phosphors in a field emission scanning electron microscope (FESEM). ZnO: Zn and other luminescent powders manifest a bright ring around the periphery of the particles: this ring enhances the contrast. Additionally, particles resting on top of others are substantially brighter than underlying ones. These phenomena are explained in terms of the combined effects of electrons backscattered out of the particles, together with light absorption by the substrate. The contrast is found to be a function of the particle size and the energy of the primary electrons. Some phosphor materials exhibit a pronounced comet-like structure at high scan rates in a CL-image, because the particle continues to emit light after the electron beam has moved to a position without phosphor material. Image analysis has been used to study the loss of brightness along the tail and hence to determine the decay time of the materials. The effect of phosphor saturation on the determination of decay times by CL-microscopy was also investigated.
RESUMEN
OBJECTIVES: Infliximab, an anti-tumor necrosis factor (TNF) monoclonal antibody, might exert some of its long-term therapeutic effects in Crohn's disease (CD) by interacting directly with cells of the immune system such as monocytes and T lymphocytes via membrane TNF and by inducing apoptosis. Accordingly, the effects of inflix-imab on monocyte apoptosis and down-regulation of proinflammatory cytokines (reverse signaling) were assessed. METHODS: To assess apoptosis, monocytes from healthy individuals (controls) and CD patients were incubated in the presence or absence of infliximab or the apoptotic agent gliotoxin for 24 hours. Annexin V staining and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-FITC nick end labeling assay were used to measure early and late apoptosis. To measure the effects of infliximab on reverse signaling, monocytes from healthy individuals pretreated in vitro with infliximab were stimulated with lipopolysaccharide or staphylococcal enterotoxin A, and the induction of the proinflammatory cytokines, TNF-alpha, interleukin (IL)-1beta, IL-6, and IL-8 was measured by reverse transcription polymerase chain reaction. The effect of in vivo infliximab treatment of monocytes was similarly determined by comparing the responses of monocytes from CD patients before and immediately after infliximab infusion. RESULTS: Infliximab did not induce apoptosis of monocytes from either healthy individuals or CD patients but rather stabilized them. However, monocytes from healthy individuals treated with infliximab in vitro, or from CD patients infused with infliximab, produced significantly less TNF and other proinflammatory cytokines when stimulated with the bacterial products lipopolysaccharide and staphylococcal enterotoxin A. CONCLUSIONS: Apoptosis of monocytes is not responsible for the therapeutic effects of infliximab. However, some of the therapeutic effects of infliximab may be caused by its ability to down-regulate proinflammatory cytokines production by monocytes exposed to bacterial antigens.
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Anticuerpos Monoclonales/farmacología , Apoptosis/efectos de los fármacos , Enfermedad de Crohn/tratamiento farmacológico , Fármacos Gastrointestinales/farmacología , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Anticuerpos Monoclonales/uso terapéutico , Estudios de Casos y Controles , Enfermedad de Crohn/patología , Fármacos Gastrointestinales/uso terapéutico , Humanos , Infliximab , Monocitos/efectos de los fármacosRESUMEN
The crystal and molecular structure of bis(ferrocenecarboxylato)(phthalocyaninato)silicon(IV) (1) is reported. The structure is compared to the only other two fully solved Si(Pc) structures. The size of the Si atom is shown to vary with the nature of the axial ligands. The implications of this finding for the chemistry and structures of the [Si(Pc)O](n)() materials is discussed. The cyclic voltammograms of 1 manifest four couples. One of these is due to the ferrocenecarboxylato ligands; this indicates that both undergo oxidation and reduction at the same potentials. Here the first study of the electrochromic behavior of a complex that also contains a redox active ligand in addition to the electrochromic center (1) is also reported. The effect of the ligand redox properties on the electrochromic properties of the Si(Pc) center is discussed.
RESUMEN
Two novel antifungals SCH 643432 (1), and 2, were isolated from the fermentation broth of a fungus taxonomically classified as a Paecilomyces varioti. These compounds were separated from the fermentation broth filtrate by adsorption on a macroreticular resin XAD-16 (Amberlite). Purification and separation of the individual compounds were achieved by trituration of the extract with dichloromethane followed by preparative HPLC using reverse phase columns. Extensive FAB (Fast Atom Bombardment) and ESI (Electro Spray) mass spectrometric studies using fragmentation of various daughter ions, NMR experiments and degradative studies helped in elucidating the structure of compound 1. Compound 2 is an isomer of SCH 643432 (1). They were identified as straight chains peptides containing several amino acids such as alanine, aminoisobutyric acid, proline, leucine, glycine and arginine. The N-terminal is terminated in a previously identified beta-keto acid, 2-methyl 3-oxo tetradecanoic acid (MOTDA). Both compounds were active against Candida albicans, other Candidas, dermatophytes and Aspergillus (Geometric Mean MIC's 4.00, 2.59, 3.56, 11.31 and 4.49, 4.00, 5.66, 16.0 microg/ml, respectively for 1 and 2).
Asunto(s)
Antifúngicos/química , Fermentación , Hongos Mitospóricos/metabolismo , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Pared Celular/efectos de los fármacosRESUMEN
Resonance Raman spectra of naturally occurring carotenoids have been obtained from nautilus, periwinkle (Littorina littorea) and clam shells under 514.5 nm excitation and these spectra are compared with the resonance Raman spectra obtained in situ from tomatoes, carrots, red peppers and saffron. The tomatoes, carrots and red peppers gave rise to resonance Raman spectra exhibiting a nu1 band at ca. 1520 cm(-1), in keeping with its assignment to carotenoids with ca. nine conjugated carbon-carbon double bonds in their main chains, whereas the resonance Raman spectrum of saffron showed a nu1 band at 1537 cm(-1) which can be assigned to crocetin, having seven conjugated carbon-carbon double bonds. A correlation between nu1 wavenumber location and effective conjugated chain length has been used to interpret the data obtained from the shells, and the wavenumber position (1522 cm(-1)) of the nu1 band of the carotenoid in the orange clam shell suggests that it contains nine conjugated double bonds in the main chain. However, the black periwinkle and nautilus shells exhibit nu1 bands at 1504 and 1496 cm(-1), respectively. On the basis of the correlation between nu1 wavenumber location and effective conjugated chain length, this indicates that they contain carotenoids with longer conjugated chains, the former having ca. 11 double bonds and the latter ca. 13 or even more. Raman spectra of the nautilus, periwinkle and clam shells also exhibited a strong band at 1085 cm(-1) and a doublet with components at 701 and 705 cm(-1), which can be assigned to biogenic calcium carbonate in the aragonite crystallographic form.
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Carotenoides/química , Animales , Moluscos/química , Espectrometría RamanRESUMEN
Nanometre sized particles of terbium and erbium co-doped yttrium oxysulfide up-converting phosphors were prepared by a urea homogeneous-precipitation method. Results from X-ray powder diffraction (XRPD), scanning electron microscopy (SEM) and photoluminescence spectroscopy studies on the microstructure and luminescent properties of the materials are reported. Upconversion emission was observed from the Er(3+) cations when particles were excited with laser light of 632.8 nm wavelength. Under these conditions no interactions between the Er(3+) cations and the Tb(3+) cations were observed. In contrast there was evidence from the Stokes emission of the Er(3+) cations under 254 nm excitation for an interaction between the Er(3+) and Tb(3+) cations reducing intensity of the latter's blue and green emission bands by cross relaxation processes.
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Erbio/química , Mediciones Luminiscentes/métodos , Nanoestructuras/química , Nanoestructuras/ultraestructura , Sulfuros/química , Terbio/química , Itrio/química , Color , Erbio/efectos de la radiación , Ensayo de Materiales , Nanoestructuras/efectos de la radiación , Tamaño de la Partícula , Sulfuros/efectos de la radiación , Terbio/efectos de la radiación , Rayos Ultravioleta , Itrio/efectos de la radiaciónRESUMEN
Y2O2S and Gd2O2S phosphor lattices activated with a range of Tb(3+) concentrations have been successfully prepared as nanoparticles and their emission properties have been characterized using SEM, XRPD, photoluminescence spectroscopy and cathodoluminescence. (5)D3-(5)D4 cross relaxation processes between Tb(3+) cations were observed in both Y2O2S and Gd2O2S as a function of Tb(3+) concentration. In the Y2O2S host lattice, the predominant emission colour shifts from blue to green with increased Tb(3+) concentration. In contrast, green emission is always predominant in Gd2O2S at Tb(3+) concentrations from 0.1 mol% to 5 mol%. This finding is explained in accordance with previous reports on the bulk materials that found the Gd2O2S lattice has a lower charge transfer state than the Y2O2S host lattice.
RESUMEN
Previous studies have shown that CD14(-/-) mice are resistant to peritoneal infection with some clinical isolates of Escherichia coli and that this resistance is accompanied by an enhanced ability to clear the bacteria; in contrast, normal mice expressing CD14 fail to clear the bacteria, causing severe sepsis and death. The enhanced clearance in CD14(-/-) mice is dependent on early neutrophil recruitment to the local foci of infection in the PC. The studies described show that neutrophil recruitment in CD14(-/-) mice occurs as a result of the local induction of the CXCL1 and CXCL2 chemokines, KC and MIP-2. Although local induction of these chemokines also occurs in normal mice, their effects on neutrophil recruitment to the PC appear to be counterbalanced by very high levels of these chemokines in the blood of normal, but not CD14(-/-), mice. Neutrophil recruitment to the PC is also inhibited in normal mice in response to LPS, which also induces high chemokine levels in the blood of normal, but not CD14(-/-), mice. However, MPLA, a monophosphorylated derivative of LPS, is able to induce early neutrophil recruitment in normal mice; this is because MPLA, unlike LPS or E. coli, induces MIP-2 and KC in the PC but not in the blood of normal mice. The pretreatment of normal mice with MPLA is able to protect them from a lethal E. coli infection. Thus, stimulation of a local CD14-independent chemokine induction pathway without triggering a systemic CD14-dependent chemokine pathway can protect against severe E. coli infections.
Asunto(s)
Movimiento Celular/inmunología , Quimiocina CXCL1/biosíntesis , Quimiocina CXCL2/biosíntesis , Infecciones por Escherichia coli/inmunología , Receptores de Lipopolisacáridos/fisiología , Neutrófilos/inmunología , Transducción de Señal/inmunología , Animales , Infecciones por Escherichia coli/metabolismo , Infecciones por Escherichia coli/patología , Regulación de la Expresión Génica/inmunología , Receptores de Lipopolisacáridos/biosíntesis , Receptores de Lipopolisacáridos/genética , Ratones , Ratones Endogámicos C57BL , Ratones Mutantes , Neutrófilos/metabolismo , Neutrófilos/patología , Índice de Severidad de la EnfermedadRESUMEN
Severe bacterial infections leading to sepsis or septic shock can be induced by bacteria that utilize different factors to drive pathogenicity and/or virulence, leading to disease in the host. One major factor expressed by all clinical isolates of gram-negative bacteria is lipopolysaccharide (LPS); a second factor expressed by some Escherichia coli strains is a K1 polysaccharide capsule. To determine the role of the CD14 LPS receptor in the pathogenic effects of naturally occurring E. coli, the responses of CD14-/- and CD14+/+ mice to three different isolates of E. coli obtained from sepsis patients were compared; two isolates express both smooth LPS and the K1 antigen, while the third isolate expresses only LPS and is negative for K1. An additional K1-positive isolate obtained from a newborn with meningitis and a K1-negative isogenic mutant of this strain were also used for these studies. CD14-/- mice were resistant to the lethal effects of the K1-negative isolates. This resistance was accompanied by significantly lower levels of systemic tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) in these mice than in CD14+/+ mice, enhanced clearance of the bacteria, and significantly fewer additional gross symptoms. In contrast, CD14-/- mice were as sensitive as CD14+/+ mice to the lethal effects of the K1-positive isolates, even though they had significantly lower levels of TNF-alpha and IL-6 than CD14+/+ mice. These studies show that different bacterial isolates can use distinctly different mechanisms to cause disease and suggest that new, nonantibiotic therapeutics need to be directed against multiple targets.
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Antígenos Bacterianos/inmunología , Cápsulas Bacterianas/inmunología , Escherichia coli/inmunología , Receptores de Lipopolisacáridos/inmunología , Polisacáridos Bacterianos/inmunología , Estructuras Animales/microbiología , Animales , Antígenos Bacterianos/genética , Cápsulas Bacterianas/genética , Recuento de Colonia Microbiana , Susceptibilidad a Enfermedades , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/mortalidad , Infecciones por Escherichia coli/fisiopatología , Eliminación de Gen , Inmunidad Innata/genética , Interleucina-6/sangre , Ratones , Ratones Endogámicos BALB C , Ratones Noqueados , Polisacáridos Bacterianos/genética , Sepsis/microbiología , Análisis de Supervivencia , Factor de Necrosis Tumoral alfa/sangreAsunto(s)
Proteínas Reguladoras de la Apoptosis/antagonistas & inhibidores , Sepsis/terapia , Anticuerpos/uso terapéutico , Antígenos CD/inmunología , Apoptosis , Proteínas Reguladoras de la Apoptosis/inmunología , Sistemas de Liberación de Medicamentos/métodos , Interacciones Huésped-Patógeno , Humanos , Terapia de Inmunosupresión , Receptor de Muerte Celular Programada 1 , Choque Séptico/terapiaRESUMEN
The interaction of LPS (endotoxin) with the CD14-TLR4 receptor complex modulates the host innate immune response. Several studies using partial structures of LPS have suggested that TLR4 determines the ligand specificity of this complex, and that CD14 indiscriminately serves to deliver the ligand to TLR4. This conclusion has been made despite observations that the response of TLR4(+/+),CD14(-/-) macrophages to LPS is very weak. To determine whether CD14 itself plays a role in specific ligand recognition, the influences of various partial structures of LPS on induction of the proinflammatory cytokine, TNF, by CD14(+/+) and CD14(-/-) macrophages were compared. These studies show that the ligand specificities of CD14(+/+) and CD14(-/-) macrophages are very different. When CD14 is present, the receptor complex shows exquisite specificity for smooth LPS, the major form expressed by Gram-negative bacteria; however, as increasing amounts of carbohydrate are removed from smooth LPS, the sensitivity of CD14(+/+) macrophages decreases as much as 500-fold. In contrast, CD14(-/-) macrophages are unable to distinguish between smooth LPS and its various partial structures. Furthermore, CD14(-/-) macrophages are 150,000-fold less sensitive than CD14(+/+) macrophages to smooth LPS. A similar ability to distinguish the differing LPS structures of various bacteria such as Bacteroides fragilis and Salmonella abortus are observed for CD14(+/+), but not CD14(-/-), macrophages. Thus, CD14(+/+), but not CD14(-/-), macrophages are highly sensitive to stimulation by natural forms of LPS and show the ability to distinguish between various LPS ligands, consistent with CD14 being a highly specific receptor.
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Receptores de Lipopolisacáridos/metabolismo , Lipopolisacáridos/metabolismo , Lipopolisacáridos/farmacología , Animales , Bacterias Gramnegativas , Ligandos , Lipopolisacáridos/química , Macrófagos/metabolismo , Ratones , Ratones Noqueados , Estructura Molecular , Especificidad por Sustrato , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
When grown on blood-containing solid media, the anaerobic periodontal pathogen Porphyromonas gingivalis produces a haem pigment, the major component of which is the mu-oxo bishaem of iron protoporphyrin IX [Smalley, Silver, Marsh and Birss (1998) Biochem. J. 331, 681-685]. In this study, mu-oxo bishaem generation by P. gingivalis from oxy- and deoxyhaemoglobin was examined. Bacterial cells were shown to convert oxyhaemoglobin into methaemoglobin, which was degraded progressively, generating a mixture of both monomeric and mu-oxo dimeric iron protoporphyrin IX. The rate of methaemoglobin formation was accelerated in the presence of bacterial cells, but was inhibited by N-ethylmaleimide and tosyl-lysylchloromethylketone. Interaction of cells with deoxyhaemoglobin resulted in formation of an iron(III) haem species (Soret gamma(max), 393 nm), identified as pure mu-oxo bishaem.