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Since treelines are generally fire-free, the trees growing there are expected to have thin bark, unless adaptation to other factors than fire results in the selection of a thick bark. Related to this is also higher proportional investment in inner bark in such an environment of infrequent fire. This study has considered stem bark thickness both in absolute and relative terms and also in the frame of the composition of outer and inner bark components of 20 tree species along an elevation transect (2100-3300 m) in high ranges of the Central Himalaya leading to treelines. The study species varied from 2.1 to 16.2 mm for total bark thickness and from 1.2 to 18.85% for relative bark thickness. The average absolute total bark thickness across the tree species decreased with elevation from forest to treeline, both when trees of all diameters (10.2 ± 0.84 mm for forest and 6.9 ± 1.79 mm for treeline) and those of the same stem diameter range (18-20 m) were compared (9.10 ± 1.30 mm for forest species and 6.38 ± 1.31 mm for treeline species). Nevertheless, the treeline bark thickness was similar to those of several forest communities considered to have comparatively thick bark. Like many other biological structures, bark carries out multiple functions; therefore, its thickness could be affected by more than one environmental factor. We suggest that the requirement of mechanical resistance to the snowfall, rainstorms, wind and adaptation to a high sunlight and UV radiations or storage of water, and non-structural carbohydrates could affect total, outer and inner bark thickness. Studies on these aspects in similar ecosystems may help understand the multi-functional attributes of the bark. For trees of comparable sizes (trees with 18-20 cm diameter at breast height) treeline species also had lower relative bark thickness (< 6%) than trees of forest below it (> 7%). The median proportion of inner bark of the total bark (70.5%) for our 20 species was more than that for savannas (~ 50%), exposed to frequent fire regime and similar to those of in cool sclerophyllous forests and temperate rain forests where fire return time is > 100 years. However, it was lower than the inner bark proportion reported for tropical rain forests. To conclude, in spite of a fire-free environment, the Himalayan treeline and adjoining forest species show mixed bark characters.
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Ecosistema , Árboles , Himalayas , Corteza de la Planta , Monitoreo del AmbienteRESUMEN
This study delves into the intricate dynamics of air pollution in the rapidly expanding northern regions of India, examining the intertwined influences of agricultural burning, industrialization, and meteorological conditions. Through comprehensive analysis of key pollutants (PM2.5, PM10, NO2, SO2, CO, O3) across ten monitoring stations in Uttar Pradesh, Haryana, Delhi, and Punjab, a consistent pattern of high pollution levels emerges, particularly notable in Delhi. Varanasi leads in SO2 and O3 concentrations, while Moradabad stands out for CO levels, and Jalandhar for SO2 concentrations. The study further elucidates the regional distribution of pollutants, with Punjab receiving significant contributions from SW, SE, and NE directions, while Haryana and Delhi predominantly face air masses from SE and NE directions. Uttar Pradesh's pollution sources are primarily local, with additional inputs from various directions. Moreover, significant negative correlations (p < 0.05) between PM10, NO2, SO2, O3, and relative humidity (RH) underscore the pivotal role of meteorological factors in shaping pollutant levels. Strong positive correlations between PM2.5 and NO2 (0.71 to 0.93) suggest shared emission sources or similar atmospheric conditions in several cities. This comprehensive understanding highlights the urgent need for targeted mitigation strategies to address the multifaceted drivers of air pollution, ensuring the protection of public health and environmental sustainability across the region.
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Contaminantes Atmosféricos , Contaminación del Aire , Ciudades , Monitoreo del Ambiente , Material Particulado , Dióxido de Azufre , Contaminantes Atmosféricos/análisis , India , Contaminación del Aire/estadística & datos numéricos , Material Particulado/análisis , Dióxido de Azufre/análisis , Dióxido de Nitrógeno/análisis , Ozono/análisis , Conceptos MeteorológicosRESUMEN
MAIN CONCLUSION: In this study, we report that peroxynitrite is necessary for ethylene-mediated aerenchyma formation in rice roots under waterlogging conditions. Plants under waterlogging stress face anoxygenic conditions which reduce their metabolism and induce several adaptations. The formation of aerenchyma is of paramount importance for the survival of plants under waterlogging conditions. Though some studies have shown the involvement of ethylene in aerenchyma formation under waterlogging conditions, the implication of peroxynitrite (ONOO-) in such a developmental process remains elusive. Here, we report an increase in aerenchyma formation in rice roots exposed to waterlogging conditions under which the number of aerenchyma cells and their size was further enhanced in response to exogenous ethephon (a donor of ethylene) or SNP (a donor of nitric oxide) treatment. Application of epicatechin (a peroxynitrite scavenger) to waterlogged plants inhibited the aerenchyma formation, signifying that ONOO- might have a role in aerenchyma formation. Interestingly, epicatechin and ethephon co-treated waterlogged plants were unable to form aerenchyma, indicating the necessity of ONOO- in ethylene-mediated aerenchyma formation under waterlogging conditions. Taken together, our results highlight the role of ONOO- in ethylene-mediated aerenchyma formation in rice and could be used in the future to develop waterlogging stress-tolerant varieties of rice.
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Catequina , Oryza , Oryza/fisiología , Ácido Peroxinitroso/metabolismo , Catequina/metabolismo , Etilenos/metabolismo , Raíces de Plantas/metabolismoRESUMEN
The generation of food waste (FW) is increasing at an alarming rate, contributing to a total of 32% of all the waste produced globally. Anaerobic digestion (AD) is an effective method for dealing with organic wastes of various compositions, like FW. Waste valorization into value-added products has increased due to the conversion of FW into biogas using AD technology. A variety of pathways are adopted by microbes to avoid unfavorable conditions in AD, including competition between sulfate-reducing bacteria and methane (CH4)-forming bacteria. Anaerobic bacteria decompose organic matter to produce biogas, a digester gas. The composition depends on the type of raw material and the method by which the digestion process is conducted. Studies have shown that the biogas produced by AD contains 65-75% CH4 and 35-45% carbon dioxide (CO2). Methanothrix soehngenii and Methanosaeta concilii are examples of species that convert acetate to CH4 and CO2. Methanobacterium bryantii, Methanobacterium thermoautotrophicum, and Methanobrevibacter arboriphilus are examples of species that produce CH4 from hydrogen and CO2. Methanobacterium formicicum, Methanobrevibacter smithii, and Methanococcus voltae are examples of species that consume formate, hydrogen, and CO2 and produce CH4. The popularity of AD has increased for the development of biorefinery because it is seen as a more environmentally acceptable alternative in comparison to physico-chemical techniques for resource and energy recovery. The review examines the possibility of using accessible FW to produce important value-added products such as organic acids (acetate/butyrate), biopolymers, and other essential value-added products.
HighlightsPopulation growth globally increases the generation of FW.FW generation, recycling, and reuse have been discussed.Biogas and bio-fertilizers can be recovered from FW through AD.
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Large-scale food waste (FW) disposal has resulted in severe environmental degradation and financial losses around the world. Although FW has a high biomass energy contents and a growing large number of national projects to recover energy from FW by anaerobic digestion (AD) are being developed. AD is a promising solution for FW management and energy generation when compared to typical disposal options including landfill disposal, incineration, and composting. AD of FW can be combined with an existing AD operation or linked to the manufacture of value-added products to reduce costs and increase income. AD is a metabolic process that requires four different types of microbes: hydrolyzers, acidogens, acetogens, and methanogens. Microbes use a variety of strategies to avoid difficult situations in the AD, such as competition for the same substrate between sulfate-reducing bacteria and methane-forming bacteria. An improved comprehension of the microbiology involved in the anaerobic digestion of FW will provide new insight into the circumstances needed to maximize this procedure, including its possibilities for use in co-digestion mechanisms. This paper reviewed the present scientific knowledge of microbial community during the AD and the connection between microbial diversity during the AD of FW.
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The purpose of this study is to identify microbial communities in pulp and paper industry sludge and their metagenomic profiling on the basis of; phylum, class, order, family, genus and species level. Results revealed that the dominant phyla in 16S rRNA Illumina Miseq analysis inside sludge were Anaerolinea, Pseudomonas, Clostridia, Bacteriodia, Gammaproteobacteria, Spirochetia, Deltaproteobacteria, Spirochaetaceae, Prolixibacteraceae and some unknown microbial strains are also dominant. Metagenomics is a molecular biology-based technology that uses bioinformatics to evaluate huge gene sequences extracted from environmental samples to assess the composition and function of microbiota. The results of metabarcoding of the V3-V4 16S rRNA regions acquired from paired-end Illumina MiSeq sequencing were used to analyze bacterial communities and structure. The present work demonstrates the potential approach to sludge treatment in the open environment via the naturally adapted microorganism, which could be an essential addition to the disposal site. In summary, these investigations indicate that the indigenous microbial community is an acceptable bioresource for remediation or detoxification following secondary treatment. This research aims at understanding the structure of microbial communities and their diversity (%) in highly contaminated sludge to perform in situ bioremediation.
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Microbiota , Aguas del Alcantarillado , Metagenoma , Metagenómica , Microbiota/genética , ARN Ribosómico 16S/genética , Aguas del Alcantarillado/microbiologíaRESUMEN
Contamination of the environment with heavy metals (HMs) has led to huge global environmental issues. Industrialization activities such as mining, manufacturing, and construction generate massive amounts of toxic waste, posing environmental risks. HMs soil pollution causes a variety of environmental issues and has a detrimental effect on both animals and plants. To remove HMs from the soil, traditional physico-chemical techniques such as immobilization, electro-remediation, stabilization, and chemical reduction are used. Moreover, the high energy, trained manpower, and hazardous chemicals required by these methods make them expensive and non-environmentally friendly. Bioremediation process, which involves microorganism-based and microorganism-associated-plant-based approaches, is an ecologically sound and cost-effective strategy for restoring HMs polluted soil. Microbes adjust their physiology to these conditions to live, which can involve significant variations in the expression of the genes. A set of genes are activated in response to toxic metals in microbes. They can also adapt by modifying their shape, fruiting bodies creating biofilms, filaments, or chemotactically migrating away from stress chemicals. Microbes including Bacillus sp., Pseudomonas sp., and Aspergillus sp. has been found to have high metals remediation and tolerance capacity of up to 98% whether isolated or in combination with plants like Helianthus annuus, Trifolium repens, and Vallisneria denseserrulata. Several of the regulatory systems that have been discovered are unique, but there is also a lot of "cross-talk" among networks. This review discusses the current state of knowledge regarding the microbial signaling responses, and the function of microbes in HMs stress resistance.
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Metales Pesados , Contaminantes del Suelo , Biodegradación Ambiental , Expresión Génica , Metales Pesados/análisis , Plantas/metabolismo , Suelo/química , Contaminantes del Suelo/análisisRESUMEN
Non-degradable pollutants have emerged as a result of industrialization, population growth, and lifestyle changes, endangering human health and the environment. Bioremediation is the process of clearing hazardous contaminants with the help of microorganisms, and cost-effective approach. The low-cost and environmentally acceptable approach to removing environmental pollutants from ecosystems is microbial bioremediation. However, to execute these different bioremediation approaches successfully, this is imperative to have a complete understanding of the variables impacting the development, metabolism, dynamics, and native microbial communities' activity in polluted areas. The emergence of new technologies like next-generation sequencing, protein and metabolic profiling, and advanced bioinformatic tools have provided critical insights into microbial communities and underlying mechanisms in environmental contaminant bioremediation. These omics approaches are meta-genomics, meta-transcriptomics, meta-proteomics, and metabolomics. Moreover, the advancements in these technologies have greatly aided in determining the effectiveness and implementing microbiological bioremediation approaches. At Environmental Protection Agency (EPA)-The government placed special emphasis on exploring how molecular and "omic" technologies may be used to determine the nature, behavior, and functions of the intrinsic microbial communities present at pollution containment systems. Several omics techniques are unquestionably more informative and valuable in elucidating the mechanism of the process and identifying the essential player's involved enzymes and their regulatory elements. This review provides an overview and description of the omics platforms that have been described in recent reports on omics approaches in bioremediation and that demonstrate the effectiveness of integrated omics approaches and their novel future use.
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Contaminantes Ambientales , Microbiota , Biodegradación Ambiental , Contaminantes Ambientales/metabolismo , Genómica/métodos , Humanos , Metabolómica/métodos , Proteómica/métodosRESUMEN
Bacteria of the genus Azospirillum include several plant associated bacteria which often promote the growth of their host plants. Although the host range of Azospirillum brasilense Sp7 is much wider than its close relative Azospirillum lipoferum 4B, it lacks the ability to efficiently utilize D-glucose for its growth. By comparing the genomes of both the species, the genes of A. lipoferum 4B responsible for conferring D-glucose utilization ability in A. brasilese Sp7 were identified by cloning individual or a combination of genes in a broad host range expression vector, mobilizing them in A. brasilense Sp7 and examining the ability of exconjugants to use D-glucose as sole carbon source for growth. These genes also included the homologs of genes involved in N-acetyl glucosamine utilization in Pseudomonas aeruginosa PAO1. A transcriptional fusion of the 5 genes encoding glucose-6-phosphate dehydrogenase and 4 components of glucose phosphotransferase system were able to improve D-glucose utilization ability in A. brasilense Sp7. The A. brasilense Sp7 strain engineered with D-glucose utilization ability showed significantly improved root colonization of rice seedling. The improvement in the ability of A. brasilense Sp7 to colonize rice roots is expected to bring benefits to rice by promoting its growth. KEY POINTS: ⢠Genes required for glucose utilization in Azospirillum lipoferum were identified. ⢠A gene cassette encoding glucose utilization was constructed. ⢠Transfer of gene cassette in A. brasilense improves glucose utilization and rice root colonization..
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Azospirillum brasilense , Azospirillum , Oryza , Azospirillum brasilense/genética , Azospirillum brasilense/metabolismo , Glucosa/metabolismoRESUMEN
Azospirillum brasilense is a plant growth-promoting bacterium that colonizes the roots of a large number of plants, including C3 and C4 grasses. Malate has been used as a preferred source of carbon for the enrichment and isolation Azospirillum spp., but the genes involved in their transport and utilization are not yet characterized. In this study, we investigated the role of the two types of dicarboxylate transporters (DctP and DctA) of A. brasilense in their ability to colonize and promote growth of the roots of a C4 grass. We found that DctP protein was distinctly upregulated in A. brasilense grown with malate as sole carbon source. Inactivation of dctP in A. brasilense led to a drastic reduction in its ability to grow on dicarboxylates and form cell aggregates. Inactivation of dctA, however, showed a marginal reduction in growth and flocculation. The growth and nitrogen fixation of a dctP and dctA double mutant of A. brasilense were severely compromised. We have shown here that DctPQM and DctA transporters play a major and a minor role in the transport of C4-dicarboxylates in A. brasilense, respectively. Studies on inoculation of the seedlings of a C4 grass, Eleusine corcana, with A. brasilense and its dicarboxylate transport mutants revealed that dicarboxylate transporters are required by A. brasilense for an efficient colonization of plant roots and their growth.
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Azospirillum brasilense , Transportadores de Ácidos Dicarboxílicos , Eleusine , Azospirillum brasilense/genética , Azospirillum brasilense/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Transportadores de Ácidos Dicarboxílicos/genética , Transportadores de Ácidos Dicarboxílicos/metabolismo , Eleusine/microbiología , Regulación Bacteriana de la Expresión Génica , Silenciador del Gen , Malatos/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/microbiologíaAsunto(s)
Criptocromos , Daño del ADN , Luz , Criptocromos/metabolismo , Criptocromos/genética , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Plantas/genética , Plantas/metabolismo , Plantas/efectos de la radiación , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Luz AzulRESUMEN
Endophytes have been explored and found to perform an important role in plant health. However, their effects on the host physiological function and disease management remain elusive. The present study aimed to assess the potential effects of endophytes, singly as well as in combination, in Withania somnifera (L.) Dunal, on various physiological parameters and systemic defense mechanisms against Alternaria alternata Seeds primed with the endophytic bacteria Bacillus amyloliquefaciens and Pseudomonas fluorescens individually and in combination demonstrated an enhanced vigor index and germination rate. Interestingly, plants treated with the two-microbe combination showed the lowest plant mortality rate (28%) under A. alternata stress. Physiological profiling of treated plants showed improved photosynthesis, respiration, transpiration, and stomatal conductance under pathogenic stress. Additionally, these endophytes not only augmented defense enzymes and antioxidant activity in treated plants but also enhanced the expression of salicylic acid- and jasmonic acid-responsive genes in the stressed plants. Reductions in reactive oxygen species (ROS) and reactive nitrogen species (RNS) along with enhanced callose deposition in host plant leaves corroborated well with the above findings. Altogether, the study provides novel insights into the underlying mechanisms behind the tripartite interaction of endophyte-A. alternata-W. somnifera and underscores their ability to boost plant health under pathogen stress.IMPORTANCEW. somnifera is well known for producing several medicinally important secondary metabolites. These secondary metabolites are required by various pharmaceutical sectors to produce life-saving drugs. However, the cultivation of W. somnifera faces severe challenge from leaf spot disease caused by A. alternata To keep pace with the rising demand for this plant and considering its capacity for cultivation under field conditions, the present study was undertaken to develop approaches to enhance production of W. somnifera through intervention using endophytes. Application of bacterial endophytes not only suppresses the pathogenicity of A. alternata but also mitigates excessive ROS/RNS generation via enhanced physiological processes and antioxidant machinery. Expression profiling of plant defense-related genes further validates the efficacy of bacterial endophytes against leaf spot disease.
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Alternaria/fisiología , Endófitos/fisiología , Enfermedades de las Plantas/microbiología , Withania/genética , Withania/microbiología , Resistencia a la Enfermedad/genética , Genes de Plantas/fisiología , Withania/inmunologíaRESUMEN
Secondary cell wall (SCW) biosynthesis is an important stage of the cotton fibre development, and its transcriptional regulation is poorly understood. We selected the Gossypium hirsutum GDSL (GhGDSL) lipase/hydrolase gene (CotAD_74480), which is expressed during SCW biosynthesis (19 through to 25 days postanthesis; DPA), for study. T1 -transgenic cotton lines expressing the ß-glucuronidase (gus) reporter under the control of a 1026-bp promoter fragment of GhGDSL (PGhGDSL ) showed 19 DPA stage-specific increase in GUS expression. 5' deletion indicated that the 194-bp fragment between -788 and -594 relative to the transcription start site was essential for this stage-specific expression. Site-directed mutagenesis of eight transcription factor binding sites within PGhGDSL demonstrated that the MYB1AT motif (AAACCA) at -603/-598 was critical for the 19 DPA-specific reporter gene expressions. Yeast one-hybrid (Y1H) analysis identified nine proteins, including GhMYB1 (CotAD_64719) that bound to the PGhGDSL promoter. Further, Y1H experiments using the 5' promoter deletions and individually mutated promoter motifs indicated that GhMYB1 interacted with PGhGDSL at MYB1AT sequence. GhMYB1 was expressed specifically in fibre from 19 DPA, overlapping with the sharp rise in GhGDSL expression, indicating that it could regulate GhGDSL during fibre development. Analysis of genes co-expressed with GhMYB1 showed that it potentially regulates a number of other 19-25 DPA-specific genes in networks including those functioning in the cell wall and precursor synthesis, but not the major polysaccharide and protein components of the fibre SCW. GhGDSL and its promoter are therefore potential tools for the improvement of cotton fibre quality traits.
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Fibra de Algodón , Regulación de la Expresión Génica de las Plantas/genética , Gossypium/genética , Proteínas de Plantas/metabolismo , Regiones Promotoras Genéticas/genética , Sitios de Unión , Pared Celular/metabolismo , Glucuronidasa , Gossypium/enzimología , Gossypium/crecimiento & desarrollo , Hidrolasas/genética , Hidrolasas/metabolismo , Lipasa/genética , Lipasa/metabolismo , Mutagénesis Sitio-Dirigida , Fenotipo , Proteínas de Plantas/genética , Unión Proteica , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
Extraction of lignin via green methods is a crucial step in promoting the bioconversion of lignocellulosic biomasses. In the present study, utilisation of natural deep eutectic solvent for the pretreatment of kenaf fibres biomass is performed. Furthermore, extracted lignin from natural deep eutectic solvent pretreated kenaf biomass was carried out and its comparative study with commercial lignin was studied. The extracted lignin was characterized and investigated through Infrared Fourier transform spectroscopy, X-ray Diffraction, thermogravimetric analysis, UV-Vis spectroscopy, and scanning electron microscopy. FTIR Spectra shows that all samples have almost same set of absorption bands with slight difference in frequencies. CHNS analysis of natural deep eutectic solvent pretreated kenaf fibre showed a slight increase in carbon % from 42.36 to 43.17% and an increase in nitrogen % from - 0.0939 to - 0.1377%. Morphological analysis of commercial lignin shows irregular/uneven surfaces whereas natural deep eutectic solvent extracted lignin shows smooth and wavy surface. EDX analysis indicated noticeable peaks for oxygen and carbon elements which are present in lignocellulosic biomass. Thermal properties showed that lignin is constant at higher temperatures due to more branching and production of extremely condensed aromatic structures. In UV-VIS spectroscopy, commercial lignin shows slightly broad peak between 300 and 400 nm due to presence of carbonyl bond whereas, natural deep eutectic solvent extracted lignin does not show up any peak in this range. XRD results showed that the crystallinity index percentage for kenaf and natural deep eutectic solvent treated kenaf was 70.33 and 69.5% respectively. Therefore, these innovative solvents will undoubtedly have significant impact on the development of clean, green, and sustainable products for biocatalysts, extraction, electrochemistry, adsorption applications.
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Hibiscus , Lignina , Lignina/química , Disolventes Eutécticos Profundos , Biomasa , Carbohidratos , Solventes/química , Carbono , HidrólisisRESUMEN
In flowering plants, the tapetum degeneration in post-meiotic anther occurs through developmental programmed cell death (dPCD), which is one of the most critical and sensitive steps for the proper development of male gametophytes and fertility. Yet the pathways of dPCD, its regulation, and its interaction with autophagy remain elusive. Here, we report that high-level expression of Arabidopsis autophagy-related gene BECLIN1 (BECN1 or AtATG6) in the tobacco tapetum prior to their dPCD resulted in developmental defects. BECN1 induces severe autophagy and multiple cytoplasm-to-vacuole pathways, which alters tapetal cell reactive oxygen species (ROS)-homeostasis that represses the tapetal dPCD. The transcriptome analysis reveals that BECN1- expression caused major changes in the pathway, resulting in altered cellular homeostasis in the tapetal cell. Moreover, BECN1-mediated autophagy reprograms the execution of tapetal PCD by altering the expression of the key developmental PCD marker genes: SCPL48, CEP1, DMP4, BFN1, MC9, EXI1, and Bcl-2 member BAG5, and BAG6. This study demonstrates that BECN1-mediated autophagy is inhibitory to the dPCD of the tapetum, but the severity of autophagy leads to autophagic death in the later stages. The delayed and altered mode of tapetal degeneration resulted in male sterility.
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Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Beclina-1/genética , Beclina-1/metabolismo , Apoptosis/genética , Homeostasis , Autofagia/genética , Regulación de la Expresión Génica de las Plantas , Flores/metabolismo , Proteínas Nucleares/genética , Chaperonas Moleculares/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismoRESUMEN
Environmental sources of chromium (Cr) such as solid waste, battery chemicals, industrial /waste, automotive exhaust emissions, mineral mining, fertilizers, and pesticides, have detrimental effects on plants. An excessive amount of Cr exposure can lead to toxic accumulations in human, animal, and plant tissues. In plants, diverse signaling molecules like hydrogen sulfide (H2S) and nitric oxide (NO) play multiple roles during Cr stress. Consequently, the molecular mechanisms of Cr toxicity in plants, such as metal binding, modifying enzyme activity, and damaging cells are examined by several studies. The reactive oxygen species (ROS) that are formed when Cr reacts with lipids, membranes, DNA, proteins, and carbohydrates are all responsible for damage caused by Cr. ROS regulate plant growth, programmed cell death (PCD), cell cycle, pathogen defense, systemic communication, abiotic stress responses, and growth. Plants accumulate Cr mostly through the root system, with very little movement to the shoots. The characterization of stress-inducible proteins and metabolites involved in Cr tolerance and cross-talk messengers has been made possible due to recent advances in metabolomics, transcriptomics, and proteomics. This review discusses Cr absorption, translocation, subcellular distribution, and cross-talk between secondary messengers as mechanisms responsible for Cr toxicity and tolerance in plants. To mitigate this problem, soil-plant systems need to be monitored for the biogeochemical behavior of Cr and the identification of secondary messengers in plants.
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Cromo , Contaminantes del Suelo , Humanos , Cromo/química , Especies Reactivas de Oxígeno/metabolismo , Plantas/metabolismo , Estrés Fisiológico , Transducción de Señal , Contaminantes del Suelo/metabolismoRESUMEN
Nutrient deficiency has resulted in impaired growth and development of the population globally. Microgreens are considered immature greens (required light for photosynthesis and growing medium) and developed from the seeds of vegetables, legumes, herbs, and cereals. These are considered "living superfood/functional food" due to the presence of chlorophyll, beta carotene, lutein, and minerals like magnesium (Mg), Potassium (K), Phosphorus (P), and Calcium (Ca). Microgreens are rich at the nutritional level and contain several phytoactive compounds (carotenoids, phenols, glucosinolates, polysterols) that are helpful for human health on Earth and in space due to their anti-microbial, anti-inflammatory, antioxidant, and anti-carcinogenic properties. Microgreens can be used as plant-based nutritive vegetarian foods that will be fruitful as a nourishing constituent in the food industryfor garnish purposes, complement flavor, texture, and color to salads, soups, flat-breads, pizzas, and sandwiches (substitute to lettuce in tacos, sandwich, burger). Good handling practices may enhance microgreens'stability, storage, and shelf-life under appropriate conditions, including light, temperature, nutrients, humidity, and substrate. Moreover, the substrate may be a nutritive liquid solution (hydroponic system) or solid medium (coco peat, coconut fiber, coir dust and husks, sand, vermicompost, sugarcane filter cake, etc.) based on a variety of microgreens. However integrated multiomics approaches alongwith nutriomics and foodomics may be explored and utilized to identify and breed most potential microgreen genotypes, biofortify including increasing the nutritional content (macro-elements:K, Ca and Mg; oligo-elements: Fe and Zn and antioxidant activity) and microgreens related other traits viz., fast growth, good nutritional values, high germination percentage, and appropriate shelf-life through the implementation of integrated approaches includes genomics, transcriptomics, sequencing-based approaches, molecular breeding, machine learning, nanoparticles, and seed priming strategiesetc.
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Abiotic stress is considered as the main culprit for reduction of global food production. Recent studies have reported GABA as a major regulator of abiotic stress and thus opening new avenues in research on emerging roles of GABA in abiotic stress acclimation in plants.
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Estrés Fisiológico , Ácido gamma-Aminobutírico , Plantas/genéticaRESUMEN
Global warming is likely to become one of the significant drivers of forest losses in the Hindu-Kush Himalaya (HKH) during the 21st century. Better understanding of how forest ecosystem will respond to global warming requires a precise knowledge of site and species specific responses to climate change. We applied dendrochronological technique to quantify and predict future growth trend of Himalayan cedar (Cedrus deodara), a tree of high commercial importance, and explored its spatial growth variability under two different climatic regimes from 17 deodar sites in the HKH. Of the two climate regimes, one is dominated by the monsoon rainfall and the other by the westerly disturbances. Analysis of tree ring width and climate (monthly temperature and precipitation) data reveals that the spring (March-May) temperature and precipitation affect the growth of deodar negatively and positively, respectively. We used Generalized Least Squares (GLS) regression model to forecast future growth of deodar by taking an ensemble of 40 General Circulation Models (GCMs) for emission scenarios RCP 4.5 and RCP 8.5. Predicted growth trends indicate the decline between 34 % and 38 % under RCP 4.5, and between 29 % and 32 % under RCP 8.5 scenarios, for the low and mid latitude sites. In contrast, a moderate increase in growth was observed in high latitude sites under the both climate scenarios. The study shows more drought stress to deodar trees growing in monsoon areas in mid-and low-latitude sites where less snow melt and low precipitation during the spring season are predicted to increase evapotranspiration. In comparison, in the higher latitude sites where there is a high snowfall due to western disturbances, the growth of deodar is predicted to increase. These findings may be used to take suitable migratory steps for the conservation of deodar in the HKH region.
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Ten dry and pregnant Murrah buffaloes were selected to investigate the effect of Asparagus racemosus feeding on hormones, metabolites, milk yield, and plasma cholesterol levels. The treatment groups of buffaloes were fed with A. racemosus (shatavari) @ 150 g/day/animal during prepartum and @ 300 g/day/animal during the postpartum period. Blood samples collected on -6, -4, -2-week, day of parturition (0), and +2, +4, and +6-week postpartum were analyzed for plasma total cholesterol, triglycerides, HDL, low-density lipoproteins (LDL), prolactin, cortisol, and blood metabolites. Milk samples collected at weekly intervals (+1, +3, +5, and 7 weeks) were analyzed for total milk fat cholesterol. Prepartum plasma cholesterol concentrations were significantly higher in treatment group over the control (P < 0.05). Mean plasma triglycerides, LDL cholesterol, HDL cholesterol, glucose, and nonesterified fatty acid (NEFA) levels varied nonsignificantly between groups. Plasma prolactin and cortisol concentrations were significantly (P < 0.01) more in treatment group than in control group. On day of parturition, plasma prolactin, cortisol, LDL, and plasma total cholesterol were higher (P < 0.01) in treatment group buffaloes in comparison to control group. A. racemosus feeding significantly (P < 0.01) increased plasma prolactin, cortisol (P < 0.01), and milk fat cholesterol (P < 0.05) without affecting total cholesterol, HDL, LDL, glucose, and NEFA concentrations. The buffaloes of treatment group produced more milk (@ 0.526 kg/animal/day) suggesting thereby that A. racemosus is galactopoietic. It was concluded that feeding of A. racemosus increases plasma prolactin and cortisol and decreased plasma total cholesterol and LDL concentration.