RESUMEN
B vitamins are some of the most commonly required biochemical cofactors in living systems. Therefore, cellular metabolism of marine vitamin-requiring (auxotrophic) phytoplankton and bacteria would likely be significantly compromised if B vitamins (thiamin B(1), riboflavin B(2), pyridoxine B(6), biotin B(7), and cobalamin B(12)) were unavailable. However, the factors controlling the synthesis, ambient concentrations, and uptake of these key organic compounds in the marine environment are still not well understood. Here, we report vertical distributions of five B vitamins (and the amino acid methionine) measured simultaneously along a latitudinal gradient through the contrasting oceanographic regimes of the southern California-Baja California coast in the Northeast Pacific margin. Although vitamin concentrations ranged from below the detection limits of our technique to 30 pM for B(2) and B(12) and to â¼500 pM for B(1), B(6), and B(7), each vitamin showed a different geographical and depth distribution. Vitamin concentrations were independent of each other and of inorganic nutrient levels, enriched primarily in the upper mesopelagic zone (depth of 100-300 m), and associated with water mass origin. Moreover, vitamin levels were below our detection limits (ranging from ≤0.18 pM for B(12) to ≤0.81 pM for B(1)) in extensive areas (100s of kilometers) of the coastal ocean, and thus may exert important constraints on the taxonomic composition of phytoplankton communities, and potentially also on rates of primary production and carbon sequestration.
Asunto(s)
Biología Marina/métodos , Fitoplancton/metabolismo , Agua de Mar/química , Agua de Mar/microbiología , Complejo Vitamínico B/metabolismo , Biotina/análisis , Biotina/metabolismo , California , Carbono/metabolismo , Cromatografía Liquida/métodos , Ecosistema , Alimentos , Metionina/análisis , Metionina/metabolismo , Oceanografía/métodos , Océanos y Mares , Fitoplancton/crecimiento & desarrollo , Riboflavina/análisis , Riboflavina/metabolismo , Salinidad , Espectrometría de Masas en Tándem/métodos , Tiamina/análisis , Tiamina/metabolismo , Vitamina B 12/análisis , Vitamina B 12/metabolismo , Vitamina B 6/análisis , Vitamina B 6/metabolismo , Complejo Vitamínico B/análisisRESUMEN
In order to establish the status of metal contamination in surface waters in the coastal ocean off Los Angeles, California, we determined their dissolved and particulate pools and compared them with levels reported in the 1970s prior the implementation of the Clean Water Act. These measurements revealed a significant reduction in particulate toxic metal concentrations in the last 33 years with decreases of â¼100-fold for Pb and â¼400-fold for Cu and Cd. Despite these reductions, the source of particulate metals appears to be primarily anthropogenic as enrichment factors were orders of magnitude above what is considered background crustal levels. Overall, dissolved trace metal concentrations in the Los Angeles coastal waters were remarkably low with values in the same range as those measured in a pristine coastal environment off Mexico's Baja California peninsula. In order to estimate the impact of metal contamination on regional phytoplankton, the internalization rate of trace metals in a locally isolated phytoplankton model organism (Synechococcus sp. CC9311) was also determined showing a rapid internalization (in the order of a few hours) for many trace metals (e.g., Ag, Cd, Cu, Pb) suggesting that those metals could potentially be incorporated into the local food webs.