Osteogenesis imperfecta in Brazilian patients.

Osteogenesis Imperfecta (OI) is a heterogeneous genetic disorder characterized by bone fragility and fracture. Mutations in 20 distinct genes can cause OI, and therefore, the genetic diagnosis of OI is frequently difficult to obtain because of the great number of genes that can be related with this disease. Studies that report the most frequently mutated genes in OI patients can help to improve molecular strategies for diagnosis of the disease. In order to characterize the mutation profile of OI in Brazilian patients, we analyzed 30 unrelated patients through SSCP screening, NGS gene panel, and/or Sanger sequencing for the 11 most frequently mutated genes in the database of mutations, including COL1A1, COL1A2, P3H1, CRTAP, PPIB, SERPINH1, SERPINF1, FKBP10, SP7, WNT1 and IFITM5. Disease-causing variants were identified in COL1A1, COL1A2, FKBP10, P3H1, and IFITM5. A total of 28 distinct mutations were identified, including seven novel changes. Our data show that the analysis of these five genes is able to detect at least 95% of causative mutations in OI disorder from Brazilian population. However, it has to be taken into considerations that distinct populations can have different frequencies of disease-causing variants. Hence, it is important to replicate this study in other groups.

Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae).

In this chapter, we report advances in tissue culture applied to Passiflora. We present reproducible protocols for somatic embryogenesis, endosperm-derived triploid production, and genetic transformation for such species knowledge generated by our research team and collaborators in the last 20 years. Our research group has pioneered the work on passion fruit somatic embryogenesis, and we directed efforts to characterize several aspects of this morphogenic pathway. Furthermore, we expanded the possibilities of understanding the molecular mechanism related to developmental phase transitions of Passiflora edulis Sims. and P. cincinnata Mast., and a transformation protocol is presented for the overexpression of microRNA156.

Xylose isomerase from Piromyces sp. E2 is a promiscuous enzyme with epimerase activity.

Xylose isomerase catalyzes the isomerization of D-xylose to D-xylulose with promiscuous activity for other saccharides including D-glucose, D-allose, and L-arabinose. The xylose isomerase from the fungus Piromyces sp. E2 (PirE2_XI) is used to engineer xylose usage by the fermenting yeast Saccharomyces cerevisiae, but its biochemical characterization is poorly understood with divergent catalytic parameters reported. We have measured the kinetic parameters of the PirE2_XI and analyzed its thermostability and pH-dependence towards different substrates. The PirE2_XI shows promiscuous activity towards D-xylose, D-glucose, D-ribose and L-arabinose with variable effects depending on different divalent ions and epimerizes D-xylose at C3 to produce D-ribulose in a substrate/product dependent ratio. The enzyme follows Michaelis-Menten kinetics for the substrates used and although KM values for D-xylose are comparable at 30 and 60 °C, the kcat/KM is three-fold greater at 60 °C. The purified PirE2_XI shows maximal activity at 65 °C in the pH range of 6.5-7.5 and is a thermostable enzyme, maintaining full activity over 48 h at 30 °C or 12 h at 60 °C. This is the first report demonstrating epimerase activity of the PirE2_XI and its ability to isomerize D-ribose and L-arabinose, and provides a comprehensive in vitro study of substrate specificity, effect of metal ions and temperature on enzyme activity and these findings advance the knowledge of the mechanism of action of this enzyme.

Photodynamic therapy as an adjunctive treatment for Grade C periodontitis in molar teeth: a preliminary trial.

OBJECTIVE: The present study aimed to evaluate the local effect of antimicrobial photodynamic therapy (aPDT) as adjunctive treatment for Grade C periodontitis in molars teeth. METHOD AND MATERIALS: Thirty-eight molar teeth from 11 patients were included in this split-mouth randomized clinical trial. The teeth were randomly divided into two groups (n = 19 each) that were submitted to conventional scaling and root planing in addition to aPDT (test group) and to scaling and root planing in addition to aPDT simulation (control group). The following periodontal parameters were obtained prior to treatment (baseline) and at 3 months after the intervention: bleeding on probing, probing depth, gingival recession, clinical attachment level, and furcation level. A 5% significance level was adopted in the statistical analysis. RESULTS: Both groups exhibited improvement in the clinical parameters (P < .001). However, the reductions in bleeding on probing and probing depth were greater in the test group (P < .001). There were no significant differences in the other clinical parameters between groups (P ≥ .130). CONCLUSIONS: Molar teeth treated with aPDT and scaling and root planing showed superior clinical improvement compared to those submitted only to scaling and root planing. aPDT may be used as adjunctive treatment for Grade C periodontitis affecting molar teeth since it seems to improve the clinical response to conventional debridement.

Desired versus actual delivery route: nursing students' perception about their type of delivery.

OBJECTIVE: To identify the perception of nursing student mothers regarding the desired type of delivery in relation to the one performed. METHOD: Qualitative research, supported by the assumptions of Humanization of Obstetric Care, developed remotely with 16 nursing students who experienced the parturition process. The data were collected through an online focus group using the Google Meet program, guided by a semi-structured script. The speeches were analyzed using the Thematic Content Analysis technique. RESULTS: An unsatisfactory discrepancy was identified between the mother's preference and the realization, predominantly the desire for the vaginal way due to academic knowledge about the benefits. However, the cesarean section was predominant as a procedure performed due to possible complications, fears, financial situation of the parturient woman and control of choice by the health professional. CONCLUSION: Perception indicates marked disagreements, with the presence of complications, fear, lack of autonomy and predominance of a hegemonic care model, reinforcing the need for expansion and application of the Humanization of Obstetric Care, as a primary condition for proper monitoring.

Description of Two New Species of Dexosarcophaga (Diptera: Sarcophagidae) From the Coastal Environments of the Brazilian Amazon.

Two new species of Dexosarcophaga Townsend (Diptera: Sarcophagidae) are described, both from the coastal zone of the Brazilian Amazon: Dexosarcophaga salgada sp. nov. and Dexosarcophaga klycyae sp. nov.

Four challenges when conducting bibliometric reviews and how to deal with them.

The evidence base in environmental sciences is increasing steadily. Environmental researchers have been challenged to handle massive volumes of data to support more comprehensive studies, assess the current status of science, and move research towards future progress. Bibliometrics can provide important insights into the research directions by providing summarized information for several end users. Here, we present an in-depth discussion on the use of bibliometric indicators to evaluate research outputs through four case studies comprising disciplines in environmental sciences. We discuss four big challenges researchers may face when conducting bibliometric reviews and how to deal with them. We also address some primary questions researchers may answer with bibliometric mapping, drawing lessons from the case studies. Lastly, we clarify some misuses of review concepts and suggest methodological principles of systematic reviews and maps to improve the overall quality of bibliometric studies.

Searching chromosome mosaicisms in 45,X Turner syndrome: how relevant is it?

OBJECTIVE: To investigate the presence of chromosome mosaicism, especially for the presence of Y derived material in 45,X women with Turner syndrome (TS). METHODS: FISH and PCR were performed for the presence of chromosome mosaicism and Y-derived-material and genetic findings were correlated to clinical data. RESULTS: Thirty-one participants were enrolled: 18 (58%) had chromosome mosaicisms (FISH), Y-derived material was found in 2. Yet, SRY primer was found with PCR in only one of them and DYZ3 was not found. The most frequent clinical findings were short or webbed neck (81,82%), high-arched palate (78%), breast hypertelorism, e cubitus valgus and genu valgus (57.6%, both), short fourth metacarpals (46.9%), epicanthic folds (43.8%), shield chest (43.8%), lymphedema (37.5%), and low set ears (34.4%). Both patients with Y-derived-material had primary amenorrhea, dyslipidemia and reached the height of 150 cm despite not treated with recombinant growth hormone (GHr). One of them showed 26% of leukocytes with Y-derived material and few clinical findings. CONCLUSION: FISH techniques proved efficient in detecting chromosome mosaicisms and Y-derived material and searching in different tissues such as mouth cells is critical due to the possibility of tissue-specific mosaicism. Phenotypical variance in TS may be a signal of chromosome mosaicisms, especially with the presence of Y-derived material.

Functional data analysis reveals asymmetrical crank torque during cycling performed at different exercise intensities.

Pedaling asymmetry is claimed as a factor of influence on injury and performance. However, the evidence is still controversial. Most previous studies determined peak torque asymmetries, which in our understanding does not consider the pattern of movement like torque profiles. Here we demonstrate that asymmetries in pedaling torque at different exercise intensities can be better described when the torque profiles are considered using functional analysis of variance than when only the peak values are analyzed. We compared peak torques and torque curves recorded while cyclists pedaled at submaximal intensities of 60%, 80%, and 95% of the maximal power output and compared data between the preferred and non-preferred legs. ANOVA showed symmetry or rather no difference in the amount of peak torque between legs, regardless of pedaling intensity. FANOVA, on the other hand, revealed significant asymmetries between legs, regardless of cycling intensity, apparently for different sections of the cycle, however, not for peak torque, either. We conclude that pedaling asymmetry cannot be quantified solely by peak torques and considering the analysis of the entire movement cycle can more accurately reflect the biomechanical movement pattern. Therefore, FANOVA data analysis could be an alternative to identify asymmetries. A novel approach as described here might be useful when combining kinetics assessment with other approaches like EMG and kinematics and help to better understand the role of pedaling asymmetries for performance and injury risks.

Early Exposure to High-Sucrose Diet Leads to Deteriorated Ovarian Health.

Background: The metabolic syndrome (MetS) is correlated with disorders of the reproductive system, such as the polycystic ovary syndrome (PCOS). While consumption of a diet rich in carbohydrates is linked to the development of MetS, it is still unclear if this diet leads to ovarian dysfunction and PCOS. Objectives: We investigated the influence of a high-sucrose diet (HSD) on the ovarian milieu of Wistar rats and studied the correlation between high consumption of sugary drinks and the prevalence of PCOS in women. Methods: Wistar rats were given a standard laboratory diet (CTR, 10% sucrose, n = 8) or HSD (HSD, 25% sucrose, n = 8) from postnatal day 21 to 120. Animals were evaluated weekly to calculate food intake, feed efficiency and weight gain. Both onset of puberty and estrous cycle were monitored. Metabolic serum biochemistry, organ morphometry and ovarian histology were performed upon euthanasia. In parallel, a fixed-effects multiple linear regression analysis was performed using data from Brazilian states (459 state-year observations) to test the correlation between the consumption of sugar-sweetened beverages (surrogate for HSD intake) and the prevalence of PCOS (surrogate for ovarian dysfunction). Results: HSD animals showed increased adipose tissue accumulation, hyperglycaemia and insulin resistance when compared to CTR. Interestingly HSD rats also entered puberty earlier than CTR. Moreover, ovaries from HSD animals had an increased number of atretic antral follicles and cystic follicles, which were correlated with the hypertrophy of periovarian adipocytes. Finally, there was a positive correlation between the intake of sugary drinks and prevalence of PCOS in women of reproductive age. Conclusions: HSD ingestion leads to ovarian dysfunction in rats and could be correlated with PCOS in women, suggesting these alterations could lead to public health issues. Therefore, we reinforce the deleterious impact of HSD to the ovarian system and suggest that the reduction of added sugars intake could be beneficial to ovarian health.

Transcultural theory in nursing care of women with infections.

OBJECTIVE: To analyze how nurses consider, in their practice of healthcare, the sociocultural context of women with sexually transmitted infections. METHOD: Qualitative research, carried out in October 2017, with ten nurses working in teams of the Estratégia de Saúde da Família (Family Health Strategy). The data were collected through semi-structured interviews, subsequently transcribed, and submitted to the organization supported by the Discourse of the Collective Subject method and analyzed according to Madeleine Leininger's transcultural theory. RESULTS: Nurses identified the sociocultural aspects of women with sexually transmitted infections and recognized the presence of influencing factors: technological, religious, economic, political, and legal, kinship and social, cultural values, and lifeways. FINAL CONSIDERATIONS: The study found that the provision of care by nurses in Primary Health Care is close to the sociocultural aspects of these women when considering the beliefs and values of each individual, especially regarding the life context and their care experiences.

Adverse effects of p-TSA-doped polypyrrole particulate exposure during zebrafish (Danio rerio) development.

Nanomaterials have been attracting attention due to the wide range of applications in nanomedicine. Polypyrrole (PPy), a conductive polymer, has been employed in the biomedical field due to its stimulus-responsive properties, although in vivo studies to assess its potential undesirable effects are limited. This study evaluated the effects of PPy doped with p-toluene sulfonic acid ((p-TSA); PPy/p-TSA) exposure (at 25, 100, 250 and 500 µg/mL) during six consecutive days on mortality, hatching, spontaneous movement, heart rate, morphology and locomotion behavior of zebrafish embryos/larvae. Additionally, PPy/p-TSA envelopment of developing embryo chorions and gene expression of a hypoxia-related marker in this context were also evaluated. No significant mortality was found; however, altered heart rate and early hatching was identified in all exposed groups at 48 hours post-fertilization (hpf). Surprisingly, with the 500 µg/mL dose, hatching initiated as early as 24 hpf. PPy/p-TSA adhered to and enveloped the chorion of embryos in a time- and dose-dependent fashion; morphological changes in body length and ocular distance were found with higher concentrations. PPy/p-TSA-exposed animals showed locomotor behavioral alterations compatible with hypoactivity. A significant increase in the turn angle with a concomitant reduction in meander was also verified at higher concentrations. Taken together, these results emphasize the adverse effects of PPy/p-TSA on zebrafish development and behavior. Some effects of PPy/p-TSA exposure were dose-dependent, and indicate specific adverse effects of PPy/p-TSA on zebrafish development and behavior.

Seletividade socioterritorial e étnico-racial: papel das políticas sociais no enfrentamento de desigualdades históricas / Socio-territorial selectivity: the role of social policies in facing historical inequalities

O presente artigo tem como objetivo geral demonstrar a importância da dimensão étnico-racial na área da habitação, enfatizando os fenômenos socioespaciais atuais, com foco na seletividade socioterritorial. Para tal, utiliza-se de ampla revisão de literatura fundamentada no materialismo histórico e dialético em Marx, bem como nas análises sobre território e espaço de Milton Santos. Assim, evidenciando as motivações deste recorte fazer-se fundamental na área a partir de um resgate histórico sobre a formação socioespacial do espaço urbano no Brasil, tornando notória a consequente seletividade socioterritorial existente nos dias atuais. A partir da contextualização histórica, as ocupações irregulares são tidas como um reflexo desta questão e as Zonas Especiais de Interesse Social (ZEIS) tornam-se um importante suporte na garantia de moradia adequada à população de baixa renda; porém, ainda um tanto ineficazes sem um olhar atento para quem são os usuários das políticas e serviços ali presentes. Ademais, se fomenta o debate sobre a dimensão étnico racial precisar estar presente desde a formação acadêmica, visto que atualmente o conteúdo referente à temática é escasso ou quase nulo

This article aims to demonstrate the importance of the ethnic-racial dimension in housing, emphasizing current socio-spatial phenomena, with a focus on socio-territorial selectivity. In order to do so, it makes use of an extensive literature review based on Marx's historical and dialectical materialism, as well as Milton Santos' analysis of territory and space. Thus, evidencing the motivations of this cut, making itself fundamental in the area from a historical review of the socio-spatial formation of urban space in Brazil, making evident the consequent socio-territorial selectivity existing today. From the historical context, irregular occupations are seen as a reflection of this issue and the Special Zones of Social Interest (ZEIS) become an important support in guaranteeing adequate housing for the low-income population; however, they are still somewhat ineffective without a careful look at who the users of the policies and services present there are. Furthermore, the debate on the racial ethnic dimension is fostered, it needs to be present since academic training, as currently the content related to the subject is scarce or almost nil

Flesh flies (Diptera: Sarcophagidae) from a white-sand habitat in the Brazilian Amazon, with the description of four new species.

The white-sand enclaves in the Amazon Basin are small areas scattered through the tropical forest, with sandy and nutrient-poor soils and an unusual vegetation type. The insect fauna of this ecosystem is poorly known, especially in the eastern Amazon. The flesh fly fauna of an area of open herbaceous white-sand vegetation known as "Campo Redondo" in the municipality of Cametá, state of Pará, was surveyed, resulting in the discovery of 43 species in 11 genera representing the subfamilies Sarcophaginae and Miltogramminae. Four new species are described: Dexosarcophaga (Dexosarcophaga) campina sp. nov., Helicobia cametaensis sp. nov., Helicobia domquixote sp. nov., and Metopia fofo sp. nov. Lepidodexia (Lepidodexia) grisea Lopes and Lepidodexia (Notochaeta) setifrons (Lopes) are newly recorded from Brazil. Dexosarcophaga (Bezzisca) ampullula (Engel), D. (Dexosarcophaga) transita Townsend and Titanogrypa (Cucullomyia) larvicida (Lopes) are newly recorded from the Brazilian Amazon.

Neck Circumference and its Correlation to Other Anthropometric Parameters and Finnish Diabetes Risk Score (FINDRISC).

BACKGROUND: The Neck Circumference (NC) is an anthropometric measure to evaluate obesity. The FINDRISC predicts the risk of developing type 2 diabetes mellitus. Our aims were to identify the mean value of NC in individuals with higher (≥15 points) and lower FINDRISC and to establish cutoff values that indicate individuals with higher FINDRISC. METHODS: It is a population-based, cross-sectional study representative of the city of Curitiba, Brazil. We studied individuals (>18 years), without diabetes mellitus, between August 2013 and August 2014. We evaluated anthropometric parameters, glycaemia, socioeconomic situation, chronic conditions, and their risk factors. In a sex-specific analysis, data are presented as mean and standard deviation. We performed Pearson's and Spearman's correlation between NC and the waist circumference, body mass index and FINDRISC. Receiver Operating Characteristic curves were estimated for NC and higher FINDRISC. Logistic regression models were built to analyze the association between higher FINDRISC and 1-SD increase in NC. RESULTS: We studied 950 individuals (621 women) with a mean age of 47.4 ± 17.6 years and body mass index of 26.2 ± 5.6 kg/m2. The mean NCs were 34.1 ± 3.1 cm in women and 38.2 ± 3.5 cm in men. Mean NC was lower in women (33.7 ± 2.9 cm vs. 35.8 ± 3.2 cm) and men (37.7 ± 3.4 cm vs. 41 ± 3.6 cm) with lower FINDRISC (p <0.001). All the correlations with NC were significant (p ≤ 0.001). The area under the curve for NC and the higher FINDRISC was 0.702 (95% CI 0.653 - 0.752) for women and 0.762 for men (95% CI 0.679 - 0.845), determining the best cutoff value of 34.5 cm for women and 39.5 cm for men to discriminate individuals with higher FINDRISC. Fully adjusted odds ratios for higher FINDRISC per 1-SD increase in NC in women and men were, respectively 1.89 (95% CI 1.53 - 2.33) and 2.86 (95% CI 1.91 - 4.29). CONCLUSION: NC is positively correlated to the body mass index, waist circumference, glycaemia, and FINDRISC scores in a population-based sample of adults. We identified the mean values of NC in higher and lower FINDRISC and established cutoff values for NC and higher FINDRISC.

O estágio curricular na atenção primária à saúde durante a pandemia de COVID-19: desafios e reinvenções para a formação em Odontologia / La pasantía curricular en atención primaria de salud durante la pandemia COVID-19: desafíos y reinvenciones para la formación en Odontología / The curricular internship in primary health care during the COVID-19 pandemic: challenges and reinventions for Dentistry education

O Decreto Legislativo n. 6/2020 reconheceu a ocorrência do estado de calamidade pública em razão da COVID-19, o que suscitou a suspensão das atividades presenciais nas instituições de ensino superior. O objetivo deste relato foi descrever e analisar a experiência de docentes e estudantes de graduação em Odontologia nos estágios curriculares na Atenção Primária à Saúde (APS) durante o Ensino Remoto Emergencial (ERE). Trata-se de um relato de experiência de docentes e monitoras com três turmas do penúltimo semestre de um curso diurno de Odontologia, no período letivo entre 2020/1 a 2021/2, totalizando 109 estudantes. Foram consultados documentos institucionais e registros dos docentes sobre as atividades de ensino. O método utilizado foi o de Sistematização deExperiências. Em agosto de 2020, com o início do ERE, o percurso do estágio foi reorganizado e a carga horária redistribuída, com os estudantes sendo preparados para posterior atuação na APS. A partir de janeiro de 2021, o estágio, que enfrentou desafios e reinvenções, foi desenvolvido na modalidade híbrida com vivências práticas nas Unidades Básicas de Saúde (UBS). Em relação aos desafios, destacam-se a capacitação para o ensino virtual; o aprendizado de lidar com as vidas perdidas; a rescisão de contratos dos profissionais das UBS, o número restrito de serviços para atender as turmas acumuladas e a expectativa de atuação clínica dos estudantes versus as restrições sanitárias. Relacionado às reinvenções, destacam-se o preenchimento e assinatura dos termos virtualmente e abertura de campos de estágio nas cidades do interior. As reconfigurações do estágio buscaram a integração entre a teoria e a prática e possibilitaram, além da formatura dos estudantes, a aquisição do arcabouço didático-pedagógico para o desenvolvimento de competências profissionais (AU).

Decreto Legislativo núm. 6/2020 reconoció la ocurrencia de un estado de calamidad pública por causa del COVID-19, que motivó la suspensión de actividades presenciales en las instituciones de educación superior. El objetivo de este informe fue describir y analizar la experiencia de profesores y estudiantes de pregrado en Odontología en prácticas curriculares en Atención Primaria a la Salud (APS) durante la Educación a Distancia de Emergencia (ERE). Se trata de un informe de la experiencia de docentes y monitores con tres clases del penúltimo semestre de una carrera diurna de Odontología, en el periodo académico comprendido entre 2020/1 y 2021/2, totalizando 109 estudiantes. Se consultaron documentos institucionales y registros docentes sobre la actividad docente. El método utilizado fue la Sistematización de Experiencias. En agosto de 2020, con el inicio del ERE, se reorganizó la ruta de prácticas y se redistribuyó la carga horaria, preparándose los estudiantes para su posterior trabajo en la APS. A partir de enero de 2021, la pasantía, que enfrentó desafíos y reinvenciones, se desarrolló en una modalidad híbrida con experiencias prácticas en Unidades Básicas de Salud (UBS). En cuanto a los desafíos, destaca la formación para la docencia virtual; aprender a lidiar con las vidas perdidas; la rescisión de contratos de los profesionales de la UBS, la restricción del número de servicios para atender las clases acumuladas y la expectativa de desempeño clínico de los estudiantes versus las restricciones sanitarias. Enrelación con las reinvenciones, las más destacadas son la cumplimentación y firma de plazos de forma virtual y la apertura de campamentos de pasantías en ciudades del interior. Las reconfiguraciones de la pasantía buscaron la integración entre teoría y práctica y permitieron, además de la graduación de los estudiantes, la adquisición del marco didáctico-pedagógico para el desarrollo de habilidades profesionales (AU).

The Law Decree n. 6/2020 recognized the occurrence of public calamity due to COVID-19, which led to the interruption of in-person activities in higher education institutions. The aim of this report is to describe and analyze the experience of professors and undergraduate Dentistry students in curricular internships in Primary Health Care (PHC) during Emergency Distance Learning (EDL). This paper reports the experience of professors and monitors with three classes from the last but one semester of adaytime Dentistry course in the academic period between 2020/1 and 2021/2, adding up to 109 students. Institutional documents and professors' records on teaching activities were consulted. The method used was Systematization of Experiences. In August 2020, with the onset of EDL, the internship was reorganized, and the workload redistributed, preparing the students for later work at PHC. After January 2021, the internship, which faced challenges and reinventions, was developed in hybrid modality with practical experiences in Basic Health Units (UBS). Regarding the challenges, the training for virtual teaching is highlighted, as well as learning to deal with lost lives, the termination of contracts of UBS professionals, the restricted number of services to assist the accumulated classes and the expectation of clinical performance of students as opposed to the health restrictions. Concerning the reinventions, the most outstanding are filling and signing terms virtually and opening internship fields in countryside cities. The reconfigurations of internship aimed at the integration between theory and practice; besides the students' graduation, it enabled the acquisition of didactic-pedagogical framework for the development of professional skills (AU).

Lesão psoriásica estigmatizante em paciente pediátrico: relato de caso / Stigmatizing psoriatic lesion in a pediatric patient responsive to immunobiological use: case report / Lesión psoriásica estigmatizante en un paciente pediátrico que responde al uso de etanercepte: reporte de un caso

A psoríase é uma patologia cutânea inflamatória e crônica que atinge de 2% a 3% da população adulta, ocorrendo antes dos 18 anos em cerca de 30% dos casos. A faixa etária pediátrica também pode ser vítima de estigmatização em virtude das lesões de pele e representa uma população com risco para problemas psiquiátricos, metabólicos e sociais. O tratamento pode ser difícil, se valendo de medicações tópicas ou sistêmicas. Contudo, o uso de imunobiológicos em crianças na sua maioria é baseado na experiência em adultos e deve ser realizado para casos específicos. Reporta-se um caso clínico de uma paciente pediátrica portadora de psoríase com lesões estigmatizantes em face que obteve boa resposta ao uso do biológico Etanercepte, mas que suspendeu tratamento após período gestacional, retomando uso de Ustequinumabe após lactação. O estudo demonstra a importância da integridade corporal e emocional do paciente e que a associação de múltiplas situações de risco e traumas constantes pode contribuir para a fragmentação da sequência das etapas de desenvolvimento e de aquisição das habilidades necessárias ao aprendizado e ao desempenho dos papéis sociais. Neste estudo também discutimos as possibilidades do tratamento da psoríase durante o ciclo gravídico-puerperal.

Psoriasis is an inflammatory and chronic skin condition that affects 2% to 3% of the adult population, occurring before the age of 18 in about 30% of cases. The pediatric age group can also fall victim to stigmatization due to skin lesions and represents a population at risk for psychiatric, metabolic, and social issues. Treatment can be challenging, relying on topical or systemic medications. However, the use of biologics in children is mostly based on adult experience and should be reserved for specific cases. A clinical case report is presented here, involving a pediatric patient with psoriasis and stigmatizing facial lesions, who showed a favorable response to use of the biologic Etanercept, but who discontinued treatment after the gestational period, resuming the use of ustekinumab after lactation. The study highlights the importance of the patient's physical and emotional well-being and suggests that the combination of multiple risk situations and ongoing traumas can contribute to the disruption of developmental stages and the acquisition of skills necessary for learning and performing social roles. In this study, we also discuss the possibilities of treating psoriasis during the pregnancy- puerperal cycle.

La psoriasis es una patología cutánea inflamatoria y crónica que afecta al 2% al 3% de la población adulta, ocurriendo antes de los 18 años en aproximadamente el 30% de los casos. El grupo de edad pediátrica también puede ser víctima de estigmatización debido a las lesiones en la piel y representa una población en riesgo de problemas psiquiátricos, metabólicos y sociales. El tratamiento puede ser difícil, utilizando medicamentos tópicos o sistémicos. Sin embargo, el uso de inmunobiológicos en niños en su mayoría se basa en la experiencia en adultos y debe reservarse para casos específicos. Se presenta un informe de un caso clínico de una paciente pediátrica con psoriasis y lesiones estigmatizantes en la cara que mostró una buena respuesta al uso del biológico Etanercept, pero que interrumpieron el tratamiento después del período gestacional, retomando el uso de ustekinumab después de la lactancia. El estudio resalta la importancia de la integridad física y emocional del paciente y sugiere que la combinación de múltiples situaciones de riesgo y traumas constantes puede contribuir a la interrupción de las etapas de desarrollo y la adquisición de habilidades necesarias para el aprendizaje y el desempeño de roles sociales. En este estudio también discutimos las posibilidades de tratar la psoriasis durante el ciclo embarazo-puerperio.

Desired versus actual delivery route: nursing students' perception about their type of delivery / El parto deseado comparado con el parto real: la percepción de los estudiantes de enfermería sobre su tipo de parto / Parto almejado versus realizado: percepção de acadêmicas de enfermagem acerca do seu tipo de parto

ABSTRACT Objective: To identify the perception of nursing student mothers regarding the desired type of delivery in relation to the one performed. Method: Qualitative research, supported by the assumptions of Humanization of Obstetric Care, developed remotely with 16 nursing students who experienced the parturition process. The data were collected through an online focus group using the Google Meet program, guided by a semi-structured script. The speeches were analyzed using the Thematic Content Analysis technique. Results: An unsatisfactory discrepancy was identified between the mother's preference and the realization, predominantly the desire for the vaginal way due to academic knowledge about the benefits. However, the cesarean section was predominant as a procedure performed due to possible complications, fears, financial situation of the parturient woman and control of choice by the health professional. Conclusion: Perception indicates marked disagreements, with the presence of complications, fear, lack of autonomy and predominance of a hegemonic care model, reinforcing the need for expansion and application of the Humanization of Obstetric Care, as a primary condition for proper monitoring.

RESUMEN Objetivo: Identificar la percepción de madres estudiantes de enfermería en cuanto al tipo de parto deseado comparado con el realizado. Método: Investigación cualitativa, apoyada en los programas de Humanización de la Asistencia Obstétrica, desarrollada de forma remota con 16 estudiantes de enfermería, que vivieron el proceso de participación. La recolección de datos se realiza a través de un grupo focal en línea mediante el programa Google Meet, guiado por un guión semiestructurado. Los discursos fueron analizados con base en la técnica de Análisis Temático del Contenido. Resultados: Se identificó una discrepancia insatisfactoria entre la preferencia de la madre y la realización, predominando el deseo de la vía vaginal en virtud de los conocimientos académicos sobre los beneficios. Por lo tanto, la cesárea fue predominante como procedimiento realizado en razón de las eventuales complicaciones, los medicamentos, la situación financiera de la parturienta y el control de la elección por parte del profesional de la salud. Conclusión: La percepción indica discordancias marcadas, con la presencia de complicaciones, medo, ausencia de autonomía y predominio de un modelo asistencial hegemónico, reforzando la necesidad de ampliación y aplicación de la Humanización de la Asistencia Obstétrica, como condición primordial para un adecuado acompañamiento.

RESUMO Objetivo: Identificar a percepção de mães acadêmicas de enfermagem quanto ao tipo de parto almejado em relação ao realizado. Método: Pesquisa qualitativa, apoiada nos pressupostos da Humanização da Assistência Obstétrica, desenvolvida de forma remota com 16 acadêmicas de enfermagem, que vivenciaram o processo de parturição. Procedeu-se a coleta por meio de grupo focal online pelo programa Google Meet, guiado por roteiro semiestruturado. Os discursos foram analisados com base na técnica de Análise Temática de Conteúdo. Resultados: Identificou-se discrepância insatisfatória entre a preferência da mãe e a realização, predominando-se o desejo pela via vaginal em virtude dos conhecimentos acadêmicos acerca dos benefícios. No entanto, a cesariana se sobressaiu enquanto procedimento realizado em razão de eventuais complicações, medos, situação financeira da parturiente e controle da escolha pelo profissional de saúde. Conclusão: A percepção indica discordâncias marcantes, com a presença de complicações, medo, ausência de autonomia e predominância de um modelo assistencial hegemônico, reforçando a necessidade de ampliação e aplicação da Humanização da Assistência Obstétrica, como condição primária para um adequado acompanhamento.

Peckia veropeso sp. nov., a flesh fly (Diptera: Sarcophagidae) from the Brazilian Amazon associated with riparian habitats.

A new species of Sarcophagidae, Peckia (Peckia) veropeso sp. nov., is described based on adult male specimens collected on organic residues in an urban open air market located on the riverside in Belém, Pará state, Brazil, and in a nearby inundated forest. This species seems to be restricted to periodically inundated riparian habitats locally known as "várzea". The distiphallus of this species is similar to that of members of the monophyletic hilifera (Aldrich)-group in the subgenus Peckia Robineau-Desvoidy. It is distinguished from the other species in the subgenus by characteristics of the male terminalia, mainly in the shape of cercus and vesica. A modified key to species of the subgenus Peckia is included.

Immunoglobulin G response to mammalian cell entry 1A (Mce1A) protein as biomarker of active tuberculosis.

Cell wall components are major determinants of virulence of Mycobacterium tuberculosis and they contribute to the induction of both humoral and cell-mediated immune response. The mammalian cell entry protein 1A (Mce1A), in the cell wall of M. tuberculosis, mediates entry of the pathogen into mammalian cells. Here, we examined serum immunoglobulin levels (IgA, IgM and total IgG) against Mce1A as a potential biomarker for diagnosis and monitoring tuberculosis (TB) treatment response. Serum samples of 39 pulmonary TB patients and 65 controls (15 healthy household contacts, 19 latently infected household contacts, 13 non-TB and 18 leprosy patients) were screened by ELISA. The median levels of all immunoglobulin classes were significantly higher in TB patients when compared with control groups. The positive test results for IgA, IgM and total IgG were 62, 54 and 82%, respectively. For comparison, routine sputum smear examination diagnosed only 26 (67%) of 39 TB cases. Sensitivities of IgA, IgM and IgG test were 59, 51.3 and 79.5%, respectively, while the specificities observed were 77.3, 83.3 and 84.4%, respectively. A significant decrease compared with baseline was also shown after TB treatment. These results suggest that circulating total IgG antibody to Mce1A could be a complementary tool to diagnosis pulmonary TB.