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INTRODUCTION: This 3-arm parallel, double-blind study aimed to evaluate the alignment efficiency of 3 different diameters of superelastic nickel-titanium (NiTi) archwires during the initial phase of orthodontic treatment. METHODS: Ninety-three patients with nonextraction treatment in the mandibular arch (using 0.022-in Roth prescription brackets) were randomly allocated into 3 groups: 0.012-in NiTi (group 1), 0.014-in NiTi (group 2), and 0.016-in NiTi (group 3). The alignment change was measured using the modified Little's irregularity index before archwire placement and every 4 weeks. The alignment efficiency of the 3 groups was analyzed. RESULTS: Ninety patients were analyzed (group 1: n = 29, group 2: n = 31, and group 3: n = 30; overall mean age, 16.6 years). The overall Wald test analysis revealed that wire type was not statistically significant (P = 0.91), whereas time exhibited significance as expected (P <0.001). Furthermore, the interaction between wire type and time was not significant (P = 0.93). The time-adjusted estimated difference between 0.014-in and 0.012-in was -0.49 (95% confidence interval, -2.83 to 1.85; P = 0.68). The time-adjusted estimated difference between 0.016-in and 0.012-in was -0.45 (95% confidence interval, -2.95 to 2.05; P = 0.72). No harm was observed. CONCLUSIONS: The clinical performance of 3 different sizes of NiTi archwires was similar. In addition, 0.014-in and 0.016-in NiTi archwires demonstrated better performances in aligning any irregularities because of a greater number of wire deformations in the 0.012-in group. REGISTRATION: IR.TBZMED.REC.1395.1039. PROTOCOL: The protocol was not published before trial commencement. FUNDING: None.
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Níquel , Alambres para Ortodoncia , Titanio , Humanos , Método Doble Ciego , Femenino , Adolescente , Masculino , Diseño de Aparato Ortodóncico , Adulto Joven , Técnicas de Movimiento Dental/instrumentación , Técnicas de Movimiento Dental/métodos , Aleaciones Dentales/química , Elasticidad , Resultado del Tratamiento , Niño , Maloclusión/terapiaRESUMEN
Aim: Retention is one of the stages of orthodontic treatment, which is an attempt to retain teeth in their corrected positions after active treatment with the use of fixed orthodontic appliances. The aim of the present study was to compare the stability of the results of orthodontic treatment and the gingival health between Hawley retainer (HR) and vacuum-formed retainer (VFR) with two different thicknesses. Materials and methods: In this randomized clinical trial, 66 patients undergoing comprehensive orthodontic treatment in a private office were evaluated after completion of treatment. The subjects were randomly assigned to three groups. At the end of orthodontic treatment, the subjects in all the groups received a fixed bonded retainer in the mandible; in the maxilla, group I received an HR, group II received a VFR with a thickness of 1.5 mm, and group III received a VFR with a thickness of 1 mm. The American Board of Orthodontics objective grading system (ABO-OGS) index was used at the end of treatment (before the delivery of the retainers) and 6 months after the use of retainers to evaluate the stability of the results of orthodontic treatment. Gingival index (GI) was used at the two above-mentioned intervals to evaluate gingival health. The ABO-OGS measurements were carried out on dental casts by a clinician who was blinded to the types of retainers the patients wore. Data were analyzed with Statistical Package for the Social Sciences (SPSS) version 20, using proper statistical analyses. Results: Six months after the delivery of retainers, ABO-OGS and GI scores with the 1.5 mm VFR were higher than those in the two other groups, with no significant differences between the three groups. There were no significant differences between the ABO-OGS scores before the delivery of retainers and 6 months after the use of retainers in any of the study groups. In the HR and 1.5 mm VFR groups, there were significant differences in GI scores between the period before the delivery of the retainers and 6 months after their delivery; however, in the 1 mm VFR group, no significant differences were observed in GI scores between the two time intervals. Conclusion: Hawley retainer and 1 mm thick and 1.5 mm thick VFRs were equally effective in preserving and stabilizing the results of orthodontic treatment during the 6-month interval after the completion of orthodontic treatment. In addition, there were no significant differences between the three retainers in relation to gingival health. Clinical significance: The VFR might be a good alternative for HR due to its better esthetic appearance and greater popularity with orthodontic patients. Keywords: American Board of Orthodontics model grading system, American Board of Orthodontics objective frading system, Gingival index, Hawley retainers, Vacuum-formed retainers.
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Retenedores Ortodóncicos , Ortodoncia Correctiva/instrumentación , Índice Periodontal , Adolescente , Adulto , Niño , Diseño de Equipo/métodos , Femenino , Enfermedades de las Encías/etiología , Humanos , Masculino , Maloclusión/terapia , Retenedores Ortodóncicos/efectos adversos , Ortodoncia Correctiva/métodos , Resultado del Tratamiento , Vacio , Adulto JovenRESUMEN
OBJECTIVES: Of the various techniques used to determine the optimum timing for growth modification treatments, the cervical vertebrae maturation method has great advantages, including validity and no need for extra X-ray exposure. Recently, the reproducibility of this method has been questioned. The aim of this study was to investigate the cause of poor reproducibility of this method and to assess the reproducibility of the clinical decisions made based on it. MATERIALS/METHODS: Seventy lateral cephalograms of Iranian patients aged 9â15 years were observed twice by five experienced orthodontists. In addition to determining the developmental stage, each single parameter involved in this method was assessed in terms of inter- and intra-observer reproducibility. In order to evaluate the reproducibility of clinical decisions based on this method, cervical vertebrae maturation staging (CVMS) I and II were considered as phase 1 and CVMS IV and V were considered as phase 3. RESULTS: By considering the clinical approach of the CVMS method, inter-observer reproducibility of this method increased from 0.48 to 0.61 (moderate to substantial) and intra-observer reproducibility enhanced from 0.72 to 0.74. LIMITATIONS: 1. Complete visualization of the first four cervical vertebrae was an inclusion criterion, which also limits the clinical application of CVMS method. 2. These results can be generalized when determining growth modification treatments solely for Class II patients. CONCLUSIONS: Difficulty in determining the morphology of C3 and C4 leads to poor reproducibility of the CVMS method. Despite this, it has acceptable reproducibility in determining the timing of functional treatment for Class II patients.
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Determinación de la Edad por el Esqueleto/normas , Vértebras Cervicales/diagnóstico por imagen , Vértebras Cervicales/crecimiento & desarrollo , Adolescente , Determinación de la Edad por el Esqueleto/métodos , Cefalometría/métodos , Cefalometría/normas , Atlas Cervical/anatomía & histología , Atlas Cervical/diagnóstico por imagen , Atlas Cervical/crecimiento & desarrollo , Vértebras Cervicales/anatomía & histología , Niño , Toma de Decisiones Clínicas , Humanos , Irán , Variaciones Dependientes del Observador , Ortodoncia/métodos , Ortodoncia/normas , Proyectos Piloto , Radiografía , Reproducibilidad de los ResultadosRESUMEN
INTRODUCTION: Determining the right time for orthodontic treatment is one of the most important factors affecting the treatment plan and its outcome. The aim of this study is to estimate the mandibular growth stage based on cervical vertebral maturation (CVM) in lateral cephalometric radiographs using artificial intelligence. Unlike previous studies, which use conventional CVM stage naming, our proposed method directly correlates cervical vertebrae with mandibular growth slope. METHODS AND MATERIALS: To conduct this study, first, information of people achieved in American Association of Orthodontics Foundation (AAOF) growth centers was assessed and after considering the entry and exit criteria, a total of 200 people, 108 women and 92 men, were included in the study. Then, the length of the mandible in the lateral cephalometric radiographs that were taken serially from the patients was calculated. The corresponding graphs were labeled based on the growth rate of the mandible in 3 stages; before the growth peak of puberty (pre-pubertal), during the growth peak of puberty (pubertal) and after the growth peak of puberty (post-pubertal). A total of 663 images were selected for evaluation using artificial intelligence. These images were evaluated with different deep learning-based artificial intelligence models considering the diagnostic measures of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV). We also employed weighted kappa statistics. RESULTS: In the diagnosis of pre-pubertal stage, the convolutional neural network (CNN) designed for this study has the higher sensitivity and NPV (0.84, 0.91 respectively) compared to ResNet-18 model. The ResNet-18 model had better performance in other diagnostic measures of the pre-pubertal stage and all measures in the pubertal and post-pubertal stages. The highest overall diagnostic accuracy was also obtained using ResNet-18 model with the amount of 87.5% compared to 81% in designed CNN. CONCLUSION: The artificial intelligence model trained in this study can receive images of cervical vertebrae and predict mandibular growth status by classifying it into one of three groups; before the growth spurt (pre-pubertal), during the growth spurt (pubertal), and after the growth spurt (post-pubertal). The highest accuracy is in post-pubertal stage with the designed networks.
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Inteligencia Artificial , Cefalometría , Vértebras Cervicales , Mandíbula , Humanos , Cefalometría/métodos , Mandíbula/crecimiento & desarrollo , Mandíbula/diagnóstico por imagen , Masculino , Femenino , Vértebras Cervicales/crecimiento & desarrollo , Vértebras Cervicales/diagnóstico por imagen , Niño , Adolescente , Pubertad/fisiología , Aprendizaje ProfundoRESUMEN
BACKGROUND: Orthodontic appliances (ligatures, braces, wires, etc.), are one of the main sources of trauma in the oral cavity. Friction between brackets and mucosa is a kind of chronic trauma that may cause buccal mucosal proliferation and ulceration and is one of the most common complaints of patients during orthodontic treatment. The aim of this study was to evaluate the changes of the lower lip epithelial cells after the removal of orthodontic fixed appliances. METHODS: Cells of lower lip oral mucosa of 31 orthodontic patients were collected by exfoliative cytology in three times: immediately (T0), 30(T1) and 60 (T2) days after debonding. Nuclear (NA) and cytoplasmic (CA) areas, NA/CA ratio, cell morphology and cellularity of smears were evaluated using cytomorphometric and cytomorphologic methods. Friedman test followed by the Wilcoxon test was used to compare the NA, CA and NA/CA values in three different times. Cell morphology and cellularity were analyzed by the Chi-square test. All statistical tests were performed with SPSS version 20. RESULTS: The NA and CA values increased significantly (P<0.001) after the removal of friction and irritation of orthodontic appliances in T1 and T2 and reached their highest level in T2. The NA/CA values did not change significantly (P=0.25). Class I smears, according to Papanicolaou classification, were observed in T0, T1, and T2. Superficial epithelial cells were predominant cell types in the lower incisor region in all times without statistically significant difference in three intervals. CONCLUSIONS: Orthodontic appliances increase keratinization of lip mucosa which is considered an adaptive reaction to the mechanical stimulus, but after removing of the appliances cellular activity rapidly increases. Keratinization is the main change observed at the end of orthodontic treatment.
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Labio , Mucosa Bucal , Células Epiteliales , Humanos , Aparatos Ortodóncicos , Aparatos Ortodóncicos FijosRESUMEN
AIM: The aim of the present study was to investigate the prevalence and distribution according to various arch length conditions of lingually erupted mandibular permanent incisors and the effect of space condition on this phenomenon with long-term follow-up of these teeth. METHODS AND MATERIALS: A total of 105 children aged 5-7.5 years with one or more permanent mandibular incisors erupting lingual to the corresponding primary incisors were included in the study. A space analysis was performed. The subjects were divided into six groups according to various space conditions. The subjects were followed for 2 years. RESULTS: In this study the prevalence of lingual eruption was 18.4%. Eighty-three point seven percent of the subjects had either equivalent space, mild space excess, or a space deficiency (not more than 3 mm). No relationship was found between groups and clinical outcomes (P>.05). Follow up of the main three groups (according to the space analysis results) revealed in the adequate space group 75% of the children lost their primary incisors without any intervention. Similar outcomes occurred in 85.7% and 57.14% of cases in the mild space excess and space deficiency groups, respectively. Considering the three main groups combined, equivalent space, mild space excess, and mild space deficiency, 70.45% of children lost their primary incisors without any intervention and 14.8% needed primary incisor extraction (17.4%, 14.3%, and 10.7% in each group, respectively). CONCLUSION: There was no predisposition toward a space deficiency or excess found in these subjects so the wait-and-see policy versus early extraction can be considered for lingually erupting permanent mandibular incisors. CLINICAL SIGNIFICANCE: Lingual eruption of mandibular incisors is a common clinical problem in the early mixed dentition period that is a source of discomfort for parents of patients with this condition. The present study provides a unique insight into the prevalence of the problem as well as the clinical decisions such as favoring retention of primary incisors as long as possible, the extraction of these primary teeth, placement of lower lingual holding arches, or referral for comprehensive orthodontic treatment. These types of data should not be regarded as standards but as illustrative of several key factors that are still not well-understood or which are still ignored.
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Incisivo/fisiopatología , Maloclusión/complicaciones , Erupción Ectópica de Dientes/etiología , Niño , Preescolar , Toma de Decisiones , Arco Dental/anatomía & histología , Arco Dental/patología , Dentición Mixta , Humanos , Incisivo/patología , Maloclusión/terapia , Mandíbula , Odontometría , Ortodoncia Correctiva/estadística & datos numéricos , Erupción Ectópica de Dientes/terapia , Extracción Dental/estadística & datos numéricos , Diente Primario/cirugíaRESUMEN
Background . The aim of this longitudinal observational study was to evaluate patients' perceptions of alignment changes during the first stage of fixed orthodontic treatment. Methods . Ninety-three non-extraction patients (mean age: 17.6 years) who were scheduled to undergo fixed-appliance treatment in the first author's private office were included. Patients assessed the alignment of their teeth subjectively using visual analogue scale at the bonding session and four, eight and 12 weeks later. The amount of Little's irregularity index at each session was calculated on stone casts. Freidman test was used to compare the "alignment changes" between different intervals. Correlation coefficients were calculated using Spearman test between Little's irregularity indices and alignment scores reported by the patients in each session. Results . No patients reported regression in alignment changes during three-month course of treatment. The final changes (from bonding session to the 12th-week visit) were smaller than the sum of the three intervals, which indicated that patients became more perceptive as the treatment progressed. Comparison of two scores reported for each session (in the same session and in the next session) revealed that patients could not recall their previous situation well. Patients do not perceive alignment changes in the same way as clinicians. Furthermore, patients who were 16 or older perceived smaller alignment changes during the first four-week period and smaller final alignment changes. Conclusion . To obtain better patient compliance and improve their motivation throughout orthodontic treatment, patientspecific measures should be undertaken, including reminding them about their initial conditions and highlighting the changes as the treatment progresses.
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Introduction: Mineral Trioxide Aggregate (MTA) is a substance with favorable physical-mechanical properties. Disodium hydrogen phosphate (DHP) is sometimes added to MTA to reduce its setting time. Therefore, this study was conducted to evaluate the effect of various ratios of liquid to powder of white MTA (WMTA) and addition of DHP on its compressive strength. Methods and Materials: One hundred and twenty samples were prepared with a two-piece stainless steel mold with a height of 6 mm and a diameter of 4 mm in order to evaluate the compressive strength where WMTA was used in 60 samples and DHP in white MTA composition (DHPWMTA) was used in other 60 samples. The compressive strength of WMTA and DHPWMTA was measured in various ratios of liquid to powder including 50, 60 and 70% and at 24 h and 21 days (n=10). Univariate Analysis of Variance test with SPSS 16 software were used to determine the difference between groups. The level of significance was set at 0.05. Results: The maximum and minimum compressive strength of WMTA groups were 63.25±1.96 (50% ratio and 21 days) and 37.79±1.28 (70% ratio and 24 h), respectively. The maximum and minimum compressive strength of DHPWMTA groups were 63.96±1.40 (60% ratio and 21 days) and 37.37±1.62 (70% ratio and 24 h), respectively. The effect of each of factors (type of material, powder to liquid ratio and time) alone were significant on the compressive strength (P<0.05). However, the interactive effect of three factors (type of material, powder to liquid ratio and time) were not statistically significant on compressive strength (P>0.05). Conclusion: Adding 2.5 wt% of DHP to white MTA increased samples compressive strength. Compressive strength in liquid to powder ratios of 50 and 60% compare to 70% and at 21 days compared to 24 h was high.
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INTRODUCTION: The use of mini-implants has increased in recent years because of their role in absolute anchorage, but the placement sites may affect the success or failure of the procedure, so it is very important to determine the appropriate and safe location for orthodontic mini-implants. On the other hand, the Cone Beam Computed Tomography (CBCT), which offers clear 3-Dimentional (3D) images, has been widely used in orthodontics and implant dentistry for surgical guidance of mini-implant placement. AIM: The aim of this retrospective study was to evaluate inter-radicular spaces between mandibular canines to second molars using cone beam 3D images. MATERIALS AND METHODS: In this retrospective cross-sectional descriptive study, maxillofacial CBCT scan data were obtained from 40 adults. The 3D images were evaluated in five axial sections at 2, 4, 6, 8 and 10 mm from the cementoenamel Junction (CEJ). To determine inter-radicular spaces, tangent lines were drawn buccolingually to the roots in axial section and the minimum distance between these two lines was measured. The data was analysed using Friedman test with SPSS(ver.13). RESULTS: Interradicular spaces of canine to second molar increased from cervical to apical direction. The maximum distance was recorded at 4 mm from the CEJ between first and second molars. CONCLUSION: According to our findings there is a distinct pattern of inter-radicular space changes in mandible. Attention to this pattern during placement of mini-implants can ensure the safety of the procedure.
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INTRODUCTION: Mineral trioxide aggregate (MTA) can be used in the treatment of irritated vital pulp and repair of root perforations. However, the initial reaction of inflammatory cells to this material and also its setting time are not ideal. Studies have shown that disodium hydrogen phosphate (DHP), decreases the setting time of MTA, with no effect on its pH. This study was undertaken to evaluate the effect of DHP on push-out bond strength of MTA at different liquid-to-powder ratios. METHODS AND MATERIALS: A total of 120 samples were prepared from the middle third of the roots of single-rooted teeth for evaluation of push-out bond strength. The push-out bond strength was measured in both groups after 72 h at different liquid-to-powder ratios, including 0.33:1, 0.5:1 and 0.6:1. Factorial ANOVA and Tukey's HSD post-hoc tests were used to compare the differences between the independent groups. Statistical significant was set at P<0.05. RESULTS: The push-out bond strengths of pure MTA and MTA+DHP groups were 10.96±5.78 and 13.32±5.03, respectively. Tukey's HSD post-hoc test revealed significant differences between the two groups. Furthermore, there were no interactive effect between material and the liquid: powder ratio. CONCLUSION: Incorporation of DHP into MTA resulted in an increase in push-out bond strength of MTA, and an increase in liquid-to-powder ratio resulted in a decrease in push-out bond strength.
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OBJECTIVE: Since there is no standard method for rebonding loose ceramic brackets, the aim of this study was to evaluate the possibility of using Er,Cr:YSGG laser to eliminate the remaining composite materials from the base of ceramic brackets and to compare the bond strength of rebonded brackets with the new ones. MATERIALS AND METHODS: Sixty-two extracted human premolars were mounted in acrylic cylinders. Thirty-one ceramic brackets were bonded, and shear bond strength was tested using Hounsfield testing machine. The remnants of the bonding material were removed from the bases of brackets using Er,Cr:YSGG laser. These brackets were rebonded to 31 fresh teeth and again shear bond strength was measured. Pattern of debonding was assessed in both cases under a stereomicroscope and graded according to ARI index. Data were analyzed with independent t-test and Fisher's exact test. RESULTS: Mean shear bond strength of the bond and rebond groups was 12.29 ± 5.46 and 10.58 ± 5.16 MPa, respectively. There were no significant differences between the two groups (p = 0.21). Pattern of bond failure was not statistically different between the two groups. CONCLUSIONS: Er,Cr:YSGG laser was effective in removing the remnants of bonding material from the base of ceramic brackets without any interference with the ceramic base itself, demonstrating that it might be a suitable method for rebonding ceramic brackets.
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Cerámica , Recubrimiento Dental Adhesivo/métodos , Láseres de Estado Sólido , Soportes Ortodóncicos , Diente Premolar , Humanos , Técnicas In VitroRESUMEN
INTRODUCTION: The aim of the present in vitro study was to compare the accuracy of cone-beam computed tomography (CBCT) in determining root canal morphology of mandibular first molars in comparison with staining and clearing technique. METHODS AND MATERIALS: CBCT images were taken from 96 extracted human mandibular first molars and the teeth were then evaluated based on Vertucci's classification to determine the root canal morphology. Afterwards, access cavities were prepared and India ink was injected into the canals with an insulin syringe. The teeth were demineralized with 5% nitric acid. Finally, the cleared teeth were evaluated under a magnifying glass at 5× magnification to determine the root canal morphology. Data were analyzed using the SPSS software. The Fisher's exact test assessed the differences between the mesial and distal canals and the Cohen's kappa test was used to assess the level of agreement between the methods. Statistical significance was defined at 0.05. RESULTS: The Kappa coefficient for agreement between the two methods evaluating canal types was 0.346 (95% CI: 0.247-0.445), which is considered a fair level of agreement based on classification of Koch and Landis. The agreement between CBCT and Vertucci's classification was 52.6% (95% CI: 45.54-59.66%), with a significantly higher agreement rate in the mesial canals (28.1%) compared to the distal canals (77.1%) (P<0.001). CONCLUSION: Under the limitations of this study, clearing technique was more accurate than CBCT in providing accurate picture of the root canal anatomy of mandibular first molars.
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INTRODUCTION: The aim of this in vitro study was to evaluate the transportation of mesiobuccal canals of mandibular first molars prepared with either BioRaCe or Mtwo rotary instruments or hand K-Flexofile, by means of cone-beam computed tomography (CBCT). METHODS AND MATERIALS: Forty-five mandibular molars were selected and randomly divided into three groups (n=15). Mesiobuccal roots of these teeth were prepared by BioRaCe, Mtwo, or hand K-Flexofile. Transportation was measured by pre- and post-operative CBCT images. Two-way ANOVA analysis was applied to detect any differences between the groups followed by the post hoc Tukey's tests. The level of significance was set at 0.05. RESULTS: The non-parametric Friedman test was used to compare the behavior of each file at 3-, 6- and 9-mm levels. There were no significant differences between different levels in Mtwo group (P=0.15); however, the differences in K-Flexofile and BioRaCe groups were significant (P>0.05). The post hoc Tukey's test revealed significant differences between BioRaCe and K-Flexofile and also between Mtwo and K-Flexofile, both in the 3-mm depths (P<0.05). CONCLUSION: Under the limitations of the present study, BioRaCe and Mtwo rotary instruments are considered suitable for canal preparation to greater apical sizes provided that the recommended sequences are observed.
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BACKGROUND AND OBJECTIVE: As the Enterococcus faecalis biofilm ages, it is likely that bacteria in mature and old biofilms will become more resistant to commonly used antibacterial irrigants. Photodynamic therapy (PDT) has been used to kill resistant bacteria organized in a biofilm. This study aimed to investigate the antimicrobial effectiveness of 1% sodium hypochlorite (NaOCl), 2.5% NaOCl, and PDT on E. faecalis biofilm at different stages of development. METHODS: In this study 4-, 6-, and 8-week-old E. faecalis biofilms were subjected to one of the following approaches: phosphate-buffered saline solution (PBS), PDT, or 1% and 2.5% NaOCl. Dentin chip suspensions were used for colony forming units (CFU) counting to estimate remaining E. faecalis counts. RESULTS: PDT and 2.5% NaOCl completely eliminated E. faecalis biofilms in three stages of biofilm development, whereas 1% NaOCl resulted in 81.88%, 85.73%, and 78.62% reductions of bacterial counts in 4-, 6-, and 8-week-old biofilms, respectively, which was significantly more than PBS. CONCLUSIONS: The bacteria in mature and old biofilms were more resistant to 1% NaOCl than were the bacteria in young biofilms. Overall survival and residual bacteria increase with biofilm aging. PDT and the 2.5% NaOCl solution were equally efficient in completely eliminating E. faecalis biofilms at all three stages.
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Biopelículas/crecimiento & desarrollo , Enterococcus faecalis/crecimiento & desarrollo , Fotoquimioterapia , Hipoclorito de Sodio/farmacología , Biopelículas/efectos de los fármacos , Enterococcus faecalis/efectos de los fármacosRESUMEN
Background and aims. The aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on Enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. Materials and methods. The modified directcontact test was used to evaluate the antibacterial effect of Adper Easy One, Bond Force, Clearfil S3 Bond, Futurabond M, G-Bond, iBond and OptiBond All-in-one adhesives on Enterococcus faecalis after aging the samples in phosphate-buffered saline for one, seven and fourteen days. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Aging effect of each adhesive was evaluated by paired-sample test. In this study, P<0.05 was considered significant. Results. All the tested adhesives exhibited antibacterial activity after one day and had significant differences with the positive control group (P<0.05). After one week, OptiBond All-in-one, iBond and Futurabond M exhibited significant differences in bacterial growth from other groups (P<0.05). There were no significant differences between the groups in two weeks (P>0.05). Conclusion. iBond exhibited the highest antibacterial effect on E. faecalis after one week. Futurabond and OptiBond All-in-one exhibited antibacterial effects against E. faecalis for one week.
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INTRODUCTION: The aim of this in vitro study was to evaluate the effect of an experimental irrigation solution, containing two different concentrations of papain, Tween 80, 2% chlorhexidine and EDTA, on removal of the smear layer. METHODS AND MATERIALS: Thirty-six single-rooted teeth were divided into two experimental groups (n=12) and two positive and negative control groups of six. The canals were prepared with BioRaCe instruments up to BR7 (60/0.02). In group 1, canals were irrigated with a combination of 1% papain, 17% EDTA, Tween 80 and 2% CHX; in group 2, canals were irrigated with a combination of 0.1% papain, 17% EDTA, Tween 80 and 2% CHX. In group 3 (the negative control), the canal was irrigated with 2.5% NaOCl during instrumentation and at the end of preparation with 1 mL of 17% EDTA was used; in group 4 (positive control), normal saline was used for irrigation. The amount of the remaining smear layer was quantified according to Hulsmann method using scanning electron microscopy (SEM). Data was analyzed by the Kruskal-Wallis and Mann-Whitney tests. RESULTS: Two-by-two comparisons of the groups revealed no significant differences in terms of smear layer removal at different canal sections between the negative control group (standard regiment for smear layer removal) and 1% papain groups (P<0.05). CONCLUSION: Under the limitations of the present study, combination of 1% papain, EDTA, 2% chlorhexidine and Tween 80 can effectively remove smear layer from canal walls.