MiR-337 suppresses pancreatic cancer development via STAT3/Wnt/ß-catenin axis.

MiRNA is an important regulator of tumorigenesis and tumor progression. MiR-337 expression was increased in pancreatic cancer tissues and it was associated with patients' survival. This study aimed to explore the influence and the potential working mechanism of miR-337 on the malignant behaviors of pancreatic cancer cells. MiR-337 expression was detected by qRT-PCR. The expression levels of STAT3, epithelial-mesenchymal transition-related genes and Wnt/ß-Catenin pathway genes were evaluated by qRT-PCR and western blot. Cell counting kit -8 and colony formation assays were conducted to examine the proliferation of AsPC-1 and SW1990 cells. Wound healing and transwell assays were performed to determine the migration and invasion of AsPC-1 and SW1990 cells. The predicted target gene of miR-337 was verified by luciferase reporter assay. The expression of miR-337 was decreased and STAT3 expression was increased in pancreatic cancer tissues as well as tumor cells. Overexpression of miR-337 suppressed proliferation, invasion and migration of AsPC-1 and SW1990 cells. MiR-337 targeted 3'UTR of STAT3 and inhibited STAT3 expression. In addition, exogenous STAT3 partially restored the inhibitory role of miR-337 on proliferation, invasion and migration of AsPC-1 and SW1990 cells. Moreover, miR-337 impeded the expression of Wnt/ß-catenin pathway-related genes. Through the saving experiment, we found that the inhibitory effect of miR-337 on AsPC-1 and SW1990 cells was abolished by the addition of LiCl. These outcomes expounded that miR-337 inactivated the Wnt/ß-catenin pathway to suppress the malignant behaviors of pancreatic cancer cells through targeting STAT3. This study may provide a novel biomarker for diagnosis and a new therapeutic target for pancreatic cancer treatment.

Reactivation performance and sludge transformation after long-term storage of Partial denitrification/Anammox (PD/A) process.

This study explores the startup of innovative Partial denitrification/Anammox (PD/A) process using long-term stored sludge (>2 years at 4 °C). Results indicate a swift recovery performance, characterized by a progressive increase in the activity of functional microorganisms with improved nitrogen volumetric loading rate during operation. Stable nitrogen removal efficiency of 99.6 % was attained at 14.2 °C under influent nitrate and ammonium of 120 and 100 mg/L, respectively. A distinctive transformation was observed as the initially black seeding sludge transitioned to brownish-red, accompanied by rapid sludge granulation with size notably increased from 263.1 µm (day 4) to 1255.0 µm (day 128), significantly contributing to the rapid PD/A performance recovery. Microbial community analysis revealed substantial increases in functional bacteria, Thauera (0.09 %-10.4 %) and Candidatus Brocadia (0.003 %-1.98 %), coinciding with enhanced nitrogen removal performance. Overall, this study underscores the viability of long-term stored PD/A sludge as a seed for rapid reactor startup, offering useful technical support to advance practical PD/A process implementation.