RESUMEN
Both lymphatic vessels and macrophages are key factors influencing the inflammatory response. During the inflammatory response, lymphatic vessels undergo dilation and growth, playing a beneficial role in alleviating inflammation by facilitating the drainage of exudate, inflammatory mediators, and leukocytes. Consequently, the promotion of lymphangiogenesis has emerged as a novel therapeutic approach to treating inflammation. Macrophages play a crucial role in promoting lymphangiogenesis by secreting several pro-lymphatic growth factors, including vascular endothelial growth factor (VEGF)-C, and undergoing transdifferentiation into lymphatic endothelial cell progenitors (LECP), which integrate into newly formed lymphatic vessels. Macrophages exhibit heterogeneity and perform diverse functions based on their phenotypes. The regulation of macrophage polarization is crucial in inflammatory responses. Notably, macrophages promote lymphangiogenesis, while lymphatic vessels, in turn, serve as a conduit for macrophages to drain out inflamed tissue and also affect macrophage polarization. Thus, there is an interactive relationship between them. In this review, we discuss current work on the effects of macrophages on lymphangiogenesis as well as lymphatic vessel recruitment of macrophages and regulation of macrophage polarization. Furthermore, we explore the roles of lymphatic vessels and macrophages in various inflammation-related diseases, emphasizing potential therapeutic targets within the context of lymphatic-macrophage interactions.
Asunto(s)
Inflamación , Linfangiogénesis , Vasos Linfáticos , Macrófagos , Macrófagos/inmunología , Macrófagos/metabolismo , Vasos Linfáticos/metabolismo , Vasos Linfáticos/patología , Humanos , Inflamación/metabolismo , Inflamación/patología , Animales , Linfangiogénesis/fisiología , Factor C de Crecimiento Endotelial Vascular/metabolismoRESUMEN
BACKGROUND: Numerous insect species undertake long-distance migrations on an enormous scale, with great implications for ecosystems. Given that take-off is the point where it all starts, whether and how the external light and internal circadian rhythm are involved in regulating the take-off behaviour remains largely unknown. Herein, we explore this issue in a migratory pest, Cnaphalocrocis medinalis, via behavioural observations and RNAi experiments. RESULTS: The results showed that C. medinalis moths took off under conditions where the light intensity gradually weakened to 0.1 lx during the afternoon or evening, and the take-off proportions under full spectrum or blue light were significantly higher than that under red and green light. The ultraviolet-A/blue light-sensitive type 1 cryptochrome gene (Cmedcry1) was significantly higher in take-off moths than that of non-take-off moths. In contrast, the expression of the light-insensitive CRY2 (Cmedcry2) and circadian genes (Cmedtim and Cmedper) showed no significant differences. After silencing Cmedcry1, the take-off proportion significantly decreased. Thus, Cmedcry1 is involved in the decrease in light intensity induced take-off behaviour in C. medinalis. CONCLUSIONS: This study can help further explain the molecular mechanisms behind insect migration, especially light perception and signal transmission during take-off phases.
Asunto(s)
Criptocromos , Proteínas de Insectos , Mariposas Nocturnas , Animales , Migración Animal , Ritmo Circadiano , Criptocromos/genética , Criptocromos/metabolismo , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Luz , Mariposas Nocturnas/fisiología , Interferencia de ARNRESUMEN
BACKGROUND: COVID-19 can have a particularly detrimental effect on patients with cancer, but no studies to date have examined if the presence, or site, of metastatic cancer is related to COVID-19 outcomes. METHODS: Using the COVID-19 and Cancer Consortium (CCC19) registry, the authors identified 10,065 patients with COVID-19 and cancer (2325 with and 7740 without metastasis at the time of COVID-19 diagnosis). The primary ordinal outcome was COVID-19 severity: not hospitalized, hospitalized but did not receive supplemental O2, hospitalized and received supplemental O2, admitted to an intensive care unit, received mechanical ventilation, or died from any cause. The authors used ordinal logistic regression models to compare COVID-19 severity by presence and specific site of metastatic cancer. They used logistic regression models to assess 30-day all-cause mortality. RESULTS: Compared to patients without metastasis, patients with metastases have increased hospitalization rates (59% vs. 49%) and higher 30 day mortality (18% vs. 9%). Patients with metastasis to bone, lung, liver, lymph nodes, and brain have significantly higher COVID-19 severity (adjusted odds ratios [ORs], 1.38, 1.59, 1.38, 1.00, and 2.21) compared to patients without metastases at those sites. Patients with metastasis to the lung have significantly higher odds of 30-day mortality (adjusted OR, 1.53; 95% confidence interval, 1.17-2.00) when adjusting for COVID-19 severity. CONCLUSIONS: Patients with metastatic cancer, especially with metastasis to the brain, are more likely to have severe outcomes after COVID-19 whereas patients with metastasis to the lung, compared to patients with cancer metastasis to other sites, have the highest 30-day mortality after COVID-19.
Asunto(s)
COVID-19 , Hospitalización , Metástasis de la Neoplasia , Neoplasias , Sistema de Registros , SARS-CoV-2 , Humanos , COVID-19/mortalidad , COVID-19/complicaciones , COVID-19/epidemiología , COVID-19/patología , Masculino , Femenino , Persona de Mediana Edad , Estudios Retrospectivos , Anciano , Hospitalización/estadística & datos numéricos , Neoplasias/patología , Neoplasias/mortalidad , SARS-CoV-2/aislamiento & purificación , Índice de Severidad de la Enfermedad , Respiración Artificial/estadística & datos numéricosRESUMEN
MAIN CONCLUSION: PlARF2 can positively regulate the seed dormancy in Paeonia lactiflora Pall. and bind the RY cis-element. Auxin, a significant phytohormone influencing seed dormancy, has been demonstrated to be regulated by auxin response factors (ARFs), key transcriptional modulators in the auxin signaling pathway. However, the role of this class of transcription factors (TFs) in perennials with complex seed dormancy mechanisms remains largely unexplored. Here, we cloned and characterized an ARF gene from Paeonia lactiflora, named PlARF2, which exhibited differential expression levels in the seeds during the process of seed dormancy release. The deduced amino acid sequence of PlARF2 had high homology with those of other plants and contained typical conserved Auxin_resp domain of the ARF family. Phylogenetic analysis revealed that PlARF2 was closely related to VvARF3 in Vitis vinifera. The subcellular localization and transcriptional activation assay showed that PlARF2 is a nuclear protein possessing transcriptional activation activity. The expression levels of dormancy-related genes in transgenic callus indicated that PlARF2 was positively correlated with the contents of PlABI3 and PlDOG1. The germination assay showed that PlARF2 promoted seed dormancy. Moreover, TF Centered Yeast one-hybrid assay (TF-Centered Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assay analysis (Dual-Luciferase) provided evidence that PlARF2 can bind to the 'CATGCATG' motif. Collectively, our findings suggest that PlARF2, as TF, could be involved in the regulation of seed dormancy and may act as a repressor of germination.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Paeonia , Filogenia , Latencia en las Plantas , Proteínas de Plantas , Paeonia/genética , Paeonia/fisiología , Paeonia/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Latencia en las Plantas/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Semillas/genética , Semillas/metabolismo , Semillas/crecimiento & desarrollo , Semillas/fisiología , Ácidos Indolacéticos/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Germinación/genética , Plantas Modificadas Genéticamente , Secuencia de AminoácidosRESUMEN
Recent investments in "clean" hydrogen as an alternative to fossil fuels are driven by anticipated climate benefits. However, most climate benefit calculations do not adequately account for all climate warming emissions and impacts over time. This study reanalyzes a previously published life cycle assessment as an illustrative example to show how the climate impacts of hydrogen deployment can be far greater than expected when including the warming effects of hydrogen emissions, observed methane emission intensities, and near-term time scales; this reduces the perceived climate benefits upon replacement of fossil fuel technologies. For example, for blue (natural gas with carbon capture) hydrogen pathways, the inclusion of upper-end hydrogen and methane emissions can yield an increase in warming in the near term by up to 50%, whereas lower-end emissions decrease warming impacts by at least 70%. For green (renewable-based electrolysis) hydrogen pathways, upper-end hydrogen emissions can reduce climate benefits in the near term by up to 25%. We also consider renewable electricity availability for green hydrogen and show that if it is not additional to what is needed to decarbonize the electric grid, there may be more warming than that seen with fossil fuel alternatives over all time scales. Assessments of hydrogen's climate impacts should include the aforementioned factors if hydrogen is to be an effective decarbonization tool.
Asunto(s)
Hidrógeno , Metano , Clima , Gas Natural , Dióxido de CarbonoRESUMEN
A simple strategy was adopted for the preparation of an antimicrobial natural rubber/graphene oxide (NR/GO) composite film modified through the use of zwitterionic polymer brushes. An NR/GO composite film with antibacterial properties was prepared using a water-based solution-casting method. The composited GO was dispersed uniformly in the NR matrix and compensated for mechanical loss in the process of modification. Based on the high bromination activity of α-H in the structure of cis-polyisoprene, the composite films were brominated on the surface through the use of N-bromosuccinimide (NBS) under the irradiation of a 40 W tungsten lamp. Polymerization was carried out on the brominated films using sulfobetaine methacrylate (SBMA) as a monomer via surface-initiated atom transfer radical polymerization (SI-ATRP). The NR/GO composite films modified using polymer brushes (PSBMAs) exhibited 99.99% antimicrobial activity for resistance to Escherichia coli and Staphylococcus aureus. A novel polymer modification strategy for NR composite materials was established effectively, and the enhanced antimicrobial properties expand the application prospects in the medical field.
RESUMEN
BACKGROUND: Persistent macrophage infiltration may lead to adverse consequences, such as calcifications and nodules in fat grafts. Lymphatic vessels, which transport inflammatory cells, are involved in regulating inflammatory responses. Less is known, however, about lymphatic vessels after fat grafting. OBJECTIVES: The aim of this study was to explore the regulation of fat graft survival by lymphatic vessels. METHODS: A common adipose graft model was constructed to assess the processes responsible for changes in the number of lymphatic vessels in grafts. Adipose tissue samples from C57/BL6 mice and green fluorescent protein-expressing mice were cross-grafted to determine the source of lymphatic vessels. The number of lymphatic vessels in the grafts was increased by treatment with vascular endothelial growth factor C, and the effects of this increase on fat grafting were evaluated. RESULTS: The number of lymphatic vessels was greater in postgrafted fat than in inguinal fat before transplantation, with lymphatic vessels in these grafts gradually transitioning from donor to recipient sources. Lymphatic vessels grew more slowly than blood vessels during early stages of grafting; during later stages, however, the number of blood vessels declined markedly, with more lymphatic vessels than blood vessels being observed 60 days after grafting. Vascular endothelial growth factor C treatment increased graft lymphatics and distant volume retention, while reducing fibrosis and oil sacs. Lymphatic vessels acted as drainage channels for macrophages, with the degree of sustained macrophage infiltration decreasing with increases in the number of lymphatic vessels. CONCLUSIONS: Increasing the number of lymphatic vessels is beneficial for fat graft survival, which may be related to a reduction in prolonged macrophage infiltration.
RESUMEN
Anionic redox chemistry can surpass theoretical limits of conventional layered oxide cathodes in energy density. A recent model system of sodium-ion batteries, O3-NaLi1/3Mn2/3O2, demonstrated full anionic redox capacity but is limited in reversibility and kinetics due to irreversible structural rearrangement and oxygen loss. Solutions to these issues are missing due to the challenging synthesis. Here, we harness the unique structural richness of sodium layered oxides and realize a controlled ratio of P2 structural intergrowth in this model compound with the overall composition maintained. The resulted O3 with 27% P2 intergrowth structure delivers an excellent initial Coulombic efficiency of 87%, comparable to the state-of-the-art Li-rich NMCs. This improvement is attributed to the effective suppression of irreversible oxygen release and structural changes, evidenced by operando Differential Electrochemical Mass Spectroscopy and X-ray Diffraction. The as-prepared intergrowth material, based on the environmentally benign Mn, exhibits a reversible capacity of 226 mAh g-1 at C/20 rate with excellent cycling stability stemming from the redox reactions of oxygen and manganese. Our work isolates the role of P2 structural intergrowth and thereby introduces a novel strategy to enhance the reversibility and kinetics of anionic redox reactions in sodium layered cathodes without compromising capacity.
RESUMEN
The intelligent response actuators based on bilayer polymer can deform under the stimulation of temperature, humidity, light, and other external environment, which is the focus of research. However, achieving multiple responses, high deformation, and programmability is still one of the challenges for these actuators. Herein, a nondetachable bilayer structure, polylactic acid-polypropylene carbonate/polyvinyl alcohol-polydopamine (PLA-PPC/PVA-PDA) multiresponse programmable actuator is prepared by a simple scraping film method. Using PLA-PPC as the solvent-driven response layer, the effects of length, thickness, shape, and solvent vapor on the deformation of PLA-PPC/PVA-PDA actuators are studied. Among them, the high curvature of the film stimulated by ethyl acetate (EA) solution is 29.85 cm-1 . Using PVA-PDA as the response layer to water molecules and infrared (IR) light, the bilayer film shows excellent curling performance. Moreover, the dynamic processes of human clothing and biomimetic squid under solvent stimulation, the picture rolling motion under water molecule stimulation, the biomimetic flower blooming and merging under the synergistic of water molecules and IR light, and the deformation process of biomimetic mimosa under the competition between water molecules and IR light are simulated, which broadens the road for the development of intelligent driving materials.
Asunto(s)
Poliésteres , Alcohol Polivinílico , Humanos , Alcohol Polivinílico/química , Temperatura , Agua , SolventesRESUMEN
The double-layer PVDF-PVC (D-PP/PP) super-hydrophobic composite membrane was prepared by the coating immersion phase separation method to enhance the mechanical properties of the composite membrane. The D-PP/PP super-hydrophobic membrane was prepared using the casting solution concentration of 12 wt% PVDF-PVC composite membrane as basement and 4% casting of PVDF-PVC coating. The contact angle of the D-PP/PP membrane was 150.4 ± 0.3°, and the scanning electron microscope showed that the surface of the D-PP/PP membrane was covered by a cross-linked micro-nano microsphere. The mechanical properties showed that the maximum tensile force of the D-PP/PP composite membrane was 2.34 N, which was 19.4% higher than that of PVDF-PVC (1.96 N). Nano-graphite was added to the coating layer in the experiment. The prepared double-layer PVDF-PVC-nano-graphite/PVDF-PVC (D-PPG/PP) composite membrane reached 153.7 ± 0.5°, the contact angle increasing by 3.3°. The SEM comparison showed that the D-PPG/PP composite membrane had a more obvious micro-nano level microsphere layer. The mechanical properties are also superior. By preparing the D-PP/PP membrane, the mechanical properties of the membrane were improved, and the super-hydrophobic property of the coating was also obtained. At the same time, it was found that adding nano-graphite to the coating layer can better improve the hydrophobic, mechanical, and self-cleaning properties of the D-PP/PP composite membrane.
RESUMEN
This paper presents a highly efficient porous adsorbent PGMA-N prepared through a series of amination reactions between polyglycidyl methacrylate (PGMA) and different polyamines. The obtained polymeric porous materials were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), specific surface area test (BET), and elemental analysis (EA). Thereinto, the PGMA-EDA porous adsorbent exhibited excellent ability to synergistically remove Cu(II) ions and sulfamethoxazole from aqueous solutions. Moreover, we studied the effects of pH, contact time, temperature, and initial concentration of pollutants on the adsorption performance of the adsorbent. The experimental results showed that the adsorption process of Cu(II) followed the pseudo-second-order kinetic model and Langmuir isotherm. The maximum adsorption capacity of PGMA-EDA for Cu(II) ions was 0.794 mmol/g. These results indicate that PGMA-EDA porous adsorbent has great potential for application in treating wastewater coexisting with heavy metals and antibiotics.
Asunto(s)
Cobre , Contaminantes Químicos del Agua , Cobre/química , Agua/química , Espectroscopía Infrarroja por Transformada de Fourier , Sulfametoxazol , Porosidad , Adsorción , Contaminantes Químicos del Agua/química , Cinética , Concentración de Iones de HidrógenoRESUMEN
An environmentally friendly pore size-controlled, superhydrophobic polylactic acid (PLA) membrane was successfully prepared by a simpler freeze solidification phase separation method (FSPS) and solution impregnation, which has application prospects in the field of oil-water separation. The pore size and structure of the membrane were adjusted by different solvent ratios and solution impregnation ratios. The PLA-FSPS membrane after solution impregnation (S-PLA-FSPS) had the characteristics of uniform pore size, superhydrophobicity and super lipophilicity, its surface roughness Ra was 338 nm, and the contact angle to water was 151°. The S-PLA-FSPS membrane was used for the oil-water separation. The membrane oil flux reached 16,084 L·m-2·h-1, and the water separation efficiency was 99.7%, which was much higher than that of other oil-water separation materials. In addition, the S-PLA-FSPS membrane could also be applied for the adsorption and removal of oil slicks and underwater heavy oil. The S-PLA-FSPS membrane has great application potential in the field of oil-water separation.
RESUMEN
In this paper, the photocatalytic degradation efficiency of typical antibiotics (norfloxacin (NOR), sulfamethoxazole (SMX) and tetracycline hydrochloride (TCH)) by Ag/CNQDs/g-C3N4 under visible light irradiation was studied. Various strategies were applied to characterize the morphology, structure and photochemical properties of the Ag/CNQDs/g-C3N4 composites. The superior photocatalytic activity of Ag/CNQDs/g-C3N4 was attributed to the wide light response range and the enhancement of interfacial charge transfer. At the same time, the effect of the influence factors (pH, Humic acid (HA) and coexisting ions) on the antibiotics degradation were also investigated. Furthermore, the electron spin resonance (ESR) technology, free radical quenching experiments, LC/MS and DFT theoretical calculations were applied to predict and identify the active groups and intermediates during the photocatalytic degradation process. In addition, Ag/CNQDs/g-C3N4 exhibited the obvious antibacterial effect to Escherichia coli due to the addition of Ag NPs. This study develops a new way for the removal of emerging antibiotic pollution from wastewaters.
Asunto(s)
Antibacterianos , Tetraciclina , Antibacterianos/química , Norfloxacino , Sulfametoxazol , Luz , CatálisisRESUMEN
OBJECTIVE: To develop an annotation model to apply natural language processing (NLP) to device adverse event reports and implement the model to evaluate the most frequently experienced events among women reporting a sterilization device removal. METHODS: We included adverse event reports from the Manufacturer and User Facility Device Experience database from January 2005 to June 2018 related to device removal following hysteroscopic sterilization. We used an iterative process to develop an annotation model that extracts six categories of desired information and applied the annotation model to train an NLP algorithm. We assessed the model performance using positive predictive value (PPV, also known as precision), sensitivity (also known as recall), and F1 score (a combined measure of PPV and sensitivity). Using extracted variables, we summarized the reporting source, the presence of prespecified and other patient and device events, additional sterilizations and other procedures performed, and time from implantation to removal. RESULTS: The overall F1 score was 91.5% for labeled items and 93.9% for distinct events after excluding duplicates. A total of 16 535 reports of device removal were analyzed. The most frequently reported patient and device events were abdominal/pelvic/genital pain (N = 13 166, 79.6%) and device dislocation/migration (N = 3180, 19.2%), respectively. Of those reporting an additional sterilization procedure, the majority had a hysterectomy or salpingectomy (N = 7932). One-fifth of the cases that had device removal timing specified reported a removal after 7 years following implantation (N = 2444/11 293). CONCLUSIONS: We present a roadmap to develop an annotation model for NLP to analyze device adverse event reports. The extracted information is informative and complements findings from previous research using administrative data.
Asunto(s)
Histeroscopía , Esterilización Tubaria , Bases de Datos Factuales , Remoción de Dispositivos/efectos adversos , Femenino , Humanos , Histeroscopía/efectos adversos , Histeroscopía/métodos , Procesamiento de Lenguaje Natural , Embarazo , Esterilización , Esterilización Tubaria/efectos adversos , Esterilización Tubaria/métodosRESUMEN
BACKGROUND: The Superficial Femoral Artery-Popliteal EvidencE Development Study Group developed contemporary objective performance goals (OPGs) for peripheral vascular interventions (PVI) for superficial femoral artery (SFA)-popliteal artery disease using the Registry Assessment of Peripheral Interventional Devices. METHODS: The Society for Vascular Surgery Vascular Quality Initiative PVI registry from January 2010 to October 2016 was used to develop OPGs based on SFA-popliteal procedures (n = 21,377) for intermittent claudication and critical limb ischemia (CLI). OPGs included 1-year rates for target lesion revascularization (TLR), major amputation, and 1 and 4-year survival rates. OPGs were calculated for the SFA and popliteal arteries and stratified by four treatments: angioplasty alone (percutaneous transluminal angioplasty [PTA]), self-expanding stenting, atherectomy, and any treatment type. Outcomes were illustrated by unadjusted Kaplan-Meier analyses. RESULTS: Cohorts included PTA (n = 7505), stenting (n = 9217), atherectomy (n = 2510) and any treatment (n = 21,377). The mean age was 69 years, 58% were male, 79% were White, and 52% had CLI. The freedom from TLR OPGs at 1 year in the SFA were 80.3% (PTA), 83.2% (stenting), 83.9% (atherectomy), and 81.9% (any treatments). The freedom from TLR OPGs at 1 year in the popliteal were 81.3% (PTA), 81.3% (stenting), 80.2% (atherectomy), and 81.1% (any treatments). The freedom from major amputation OPGs at 1 year after SFA PVI were 93.4% (PTA), 95.7% (stenting), 95.1% (atherectomy), and 94.8% (any treatments). The freedom from major amputation OPG at 1 year after popliteal PVI were 90.5% (PTA), 93.7% (stenting), 91.8% (atherectomy), and 91.8%, (any treatments). The 4-year survival OPGs after SFA PVI were 76% (PTA), 80% (stenting), 82% (atherectomy), and 79% (any treatments), and for the popliteal artery were 72% (PTA), 77% (stenting), 82% (atherectomy), and 75% (any treatment). On a multivariable analysis, which included patient-level, leg-level, and lesion-level covariates, CLI was the single independent factor associated with increased TLR, amputation, and mortality. CONCLUSIONS: The Superficial Femoral Artery-Popliteal EvidencE Development OPGs define a new, contemporary benchmark for SFA-popliteal interventions using a large subset of real-world evidence to inform more efficient peripheral device clinical trial designs to support regulatory and clinical decision-making. It is appropriate to discuss proposals intended for regulatory approval with the US Food and Drug Administration to refine the OPG to match the specific trial population. The OPGs may be updated using coordinated registry networks to assess long-term real-world device performance.
Asunto(s)
Benchmarking , Procedimientos Endovasculares/instrumentación , Arteria Femoral , Claudicación Intermitente/terapia , Isquemia/terapia , Enfermedad Arterial Periférica/terapia , Arteria Poplítea , Indicadores de Calidad de la Atención de Salud , Anciano , Anciano de 80 o más Años , Amputación Quirúrgica , Benchmarking/normas , Enfermedad Crítica , Procedimientos Endovasculares/efectos adversos , Procedimientos Endovasculares/mortalidad , Procedimientos Endovasculares/normas , Femenino , Arteria Femoral/diagnóstico por imagen , Arteria Femoral/fisiopatología , Mortalidad Hospitalaria , Humanos , Claudicación Intermitente/diagnóstico por imagen , Claudicación Intermitente/mortalidad , Claudicación Intermitente/fisiopatología , Isquemia/diagnóstico por imagen , Isquemia/mortalidad , Isquemia/fisiopatología , Recuperación del Miembro , Masculino , Persona de Mediana Edad , Enfermedad Arterial Periférica/diagnóstico por imagen , Enfermedad Arterial Periférica/mortalidad , Enfermedad Arterial Periférica/fisiopatología , Arteria Poplítea/diagnóstico por imagen , Arteria Poplítea/fisiopatología , Indicadores de Calidad de la Atención de Salud/normas , Sistema de Registros , Estudios Retrospectivos , Medición de Riesgo , Factores de Riesgo , Factores de Tiempo , Resultado del Tratamiento , Estados UnidosRESUMEN
INTRODUCTION: Immunocompromised patients are postulated to have higher rates of post-operative infection. We sought to determine if inflatable penile prosthesis (IPP) reoperation rates (due to infection, erosion, device malfunction or patient dissatisfaction) are higher among immunocompromised men. METHODS: We analyzed men who underwent initial IPP insertion from 2000 to 2016 in the New York Statewide Planning and Research Cooperative System database. Immunocompromised patients were propensity-score matched in a 1:3 fashion with immunocompetent patients. We estimated and compared reoperation rates (including removal, reoperation due to infection, revision, or replacement of an IPP after an index procedure) at 30 days, 90 days, 1 year and 3 years of follow up between immunocompromised men and controls by performing a Kaplan Meier analysis and Log-rank tests. Cox proportional hazards models were built to examine the overall association between immune deficient status and the risk of reoperation. MAIN OUTCOME MEASURE: Reoperation rate and time to reoperation after index IPP placement. RESULTS: A total of 245 immunocompromised patients who received an initial IPP between 2000 and 2016 were identified. After propensity score matching, we analyzed 235 immunocompromised men and 705 controls. There was no difference in overall reoperation rates between immunocompromised men and controls within any time period assessed (30 days, 90 days, 1 year, or 3 years). In our Cox proportional hazards model, the hazards of overall reoperation, removal, or revision/replacement (HR 1.11 [95% CI 0.74-1.67], HR 1.58 [95% CI 0.90-2.79)], and HR 0.83 [95% CI 0.47-1.45], respectively) were not significant different between immunocompromised men and controls. Reoperation due to infection was also not significantly different between immunocompromised and immunocompetent men (HR 2.06 [95% CI 0.97-4.40]). STRENGTHS & LIMITATIONS: This study is strengthened by its size as the largest cohort of immunocompromised men treated with IPP to date in the literature, but is limited by the retrospective nature of the database which may introduce selection bias and by the low event rate for IPP reoperation. CONCLUSIONS: Reoperation rates, including those due to infection, are not significantly different between immunocompromised men and immunocompetent controls. Therefore, immune status in appropriately selected candidates does not appear to place patients at substantially higher risk of explant or revision. Gaffney CD, Fainberg J, Aboukhshaba A, et al. Immune Deficiency Does Not Increase Inflatable Penile Prosthesis Reoperation Rates. J Sex Med 2021;18:1427-1433.
Asunto(s)
Disfunción Eréctil , Implantación de Pene , Prótesis de Pene , Disfunción Eréctil/cirugía , Humanos , Masculino , Prótesis de Pene/efectos adversos , Reoperación , Estudios RetrospectivosRESUMEN
Greenhouse gas (GHG) emissions from constructed wetlands (CWs) lower the environmental and ecological benefits of CWs and thus have raised increasing environmental concern. To prevent GHGs emissions, it is important to assess and quantify the correlation of GHGs emission and microbial carbon and nitrogen transformations. In this study, two typical wetland substrate samples (mud sampled from Xiaomei River CW and sand sampled from Dongwen River CW) were used to build lab-scale vertical subsurface flow CW microcosms, labeled as XRCW and DRCW, respectively. The mean COD removal rate of the DRCW group (76.1%) was higher than that of XRCW group (60.6%). Both groups achieved a high extent of nitrogen nutrient removal, indicating a higher metabolic activity of nitrifying and denitrifying microorganisms in the system, especially in XRCW. The mean emission fluxes of N2O, CH4 and CO2 in the XRCW group were 52.7 µg/m2-h, 1.6 mg/m2-h and 100.4 mg/m2-h, which were higher than that in the DRCW group (30.0 µg/m2-h, 1.0 mg/m2-h and 28.0 mg/m2-h, respectively). The relation of GHG emissions to microbial carbon and nitrogen transformation was assessed by genomics and functional analysis. The release of GHGs by the XRCW group had a positive correlation with the relative abundance of Proteobacteria, while for the DRCW group a positive correlation was found with the relative abundance of Cyanobacteria. Nitrogen fixation by Cyanobacteria could be an approach to reduce GHG emissions. The release of CH4 and CO2 was positively correlated with glucose metabolism. N2O gas emission was affected by the species of denitrifiers. This study is of great importance to clarify the emissions of GHGs in vertical subsurface flow CWs, as it is relating to microbial carbon and nitrogen transformation. The connection is of great significance to control the emission of GHGs in wetlands.
Asunto(s)
Gases de Efecto Invernadero , Carbono , Dióxido de Carbono/análisis , Genómica , Gases de Efecto Invernadero/análisis , Metano/análisis , Nitrógeno , Óxido Nitroso/análisis , HumedalesRESUMEN
PURPOSE: We aimed to determine the real world safety and cost of third line overactive bladder therapies, including onabotulinumtoxinA and sacral neuromodulation. MATERIALS AND METHODS: We performed an all-inclusive, population based cohort study of third line therapies of overactive bladder (sacral neuromodulation or onabotulinumtoxinA) using the statewide surgical data captured in the New York Statewide Planning and Research Cooperative System. The main outcome measures were 30-day safety events, and 1 and 3-year health care utilization costs. Propensity score matching was done to control for confounding factors and comparative analyses of safety events were also performed. RESULTS: Our cohort included 2,680 patients, of whom 1,328 underwent sacral neuromodulation and 1,352 received onabotulinumtoxinA from January 1, 2013 through December 31, 2016. Average ± SD age was 61.7 ± 16.3 years and 82.7% of the patients were female. Sacral neuromodulation implantation led to re-intervention in 15.8% of cases within 1 year and in 26.1% at 3 years. In this comparative analysis patients who received onabotulinumtoxinA therapy were at higher risk for urinary tract infection, hematuria, urinary retention and an emergency room visit compared to those treated with sacral neuromodulation. The overall cost of onabotulinumtoxinA was lower than the cost of the sacral neuromodulation device (cost at 1 year $2,896 vs $15,343 and at 3 years $3,454 vs $16,189, each p <0.01). CONCLUSIONS: Sacral neuromodulation implantation was more expensive than onabotulinumtoxinA injection. However, patients who underwent sacral neuromodulation had a lower complication rate than patients treated with onabotulinumtoxinA. A quality improvement collective database must be created to track information on onabotulinumtoxinA and sacral neuromodulation treatment. This would help generate better performance and comparative data for patient and physician decision making.
Asunto(s)
Toxinas Botulínicas Tipo A/uso terapéutico , Fármacos Neuromusculares/uso terapéutico , Estimulación Eléctrica Transcutánea del Nervio/métodos , Vejiga Urinaria Hiperactiva/terapia , Anciano , Toxinas Botulínicas Tipo A/economía , Femenino , Humanos , Plexo Lumbosacro , Masculino , Fármacos Neuromusculares/economía , New York , Seguridad del Paciente , Puntaje de Propensión , Estimulación Eléctrica Transcutánea del Nervio/economía , Vejiga Urinaria Hiperactiva/economíaRESUMEN
PURPOSE: Inflatable penile prostheses and artificial urinary sphincters are used to treat men with erectile dysfunction and stress urinary incontinence, respectively. After prostate cancer treatment men often experience erectile dysfunction and stress urinary incontinence. Dual prosthetic implantation can improve the quality of life of these men. We evaluated reoperation outcomes in men who underwent dual implantation compared to each device implanted individually. MATERIALS AND METHODS: We queried the SPARCS (New York State Department of Health Statewide Planning and Research Cooperative) database for men who underwent inflatable penile prosthesis and/or artificial urinary sphincter insertion between 2000 and 2014. The primary outcomes were the inflatable penile prosthesis and artificial urinary sphincter reoperation rates (revision, replacement or removal). Multivariable regression analysis was performed to assess the association of dual implantation with reoperation. Adjusted time to event analysis was also performed. RESULTS: Median followup in the inflatable penile prosthesis cohort was 66 months (IQR 25-118) and in the artificial urinary sphincter cohort it was 69 months (IQR 27-121). Compared with men who received a penile prosthesis alone those with a penile prosthesis and an artificial urinary sphincter had a higher likelihood of undergoing inflatable penile prosthesis reoperation at 1 year (OR 2.08, 95% CI 1.32-3.27, p <0.01) and 3 years (OR 2.60, 95% CI 1.69-3.99, p <0.01). Compared with an artificial urinary sphincter alone patients with an inflatable penile prosthesis and an artificial urinary sphincter did not have a higher likelihood of undergoing artificial urinary sphincter reoperation at 1 year (p = 0.76) or 3 years (p = 0.73). CONCLUSIONS: Combined inflatable penile prosthesis and artificial urinary sphincter insertion portends a higher likelihood of inflatable penile prosthesis reoperation at 1 and 3 years. However, artificial urinary sphincter outcomes remain comparable. These findings should be used to better counsel patients about the risk of reoperation when undergoing dual implantation.