RESUMEN
BACKGROUND: Pustulosis palmaris et plantaris (PPP) is a chronic, inflammatory, autoimmune-type disease characterized by sterile pustules of skin. The skin inflammation is influenced by several factors such as drugs, sunlight, metabolic and psychogenic factors as well as metal allergy. Here, we report a rare case that intensive periodontal treatment might have contributed to the improvement of skin inflammation. RESULTS: Skin inflammation regressed 1 month after intensive periodontal treatment. Both CD4/CD8 ratio and % of B cells in the blood sample were slightly decreased corresponding to the improvement of periodontal inflammation. CONCLUSIONS: Infection control of periodontal lesions might be one of attractive therapeutic targets in management of PPP.
Asunto(s)
Periodontitis Crónica/complicaciones , Periodontitis Crónica/tratamiento farmacológico , Psoriasis/tratamiento farmacológico , Psoriasis/etiología , Linfocitos B , Proteína C-Reactiva/análisis , Relación CD4-CD8 , Humanos , Recuento de Leucocitos , Masculino , Persona de Mediana Edad , Psoriasis/patología , Piel/patologíaRESUMEN
BACKGROUND: Cyclosporin A (CsA) is an immunosuppressant with side effects including gingival hyperplasia. Sarcoidosis is a systemic disease characterized by granulomas. Here, we report on a rare case of sarcoidosis with gingival hyperplasia to clarify whether clinical observation corresponds to in vitro results. METHODS: Gingival fibroblasts (HGFs) were isolated from healthy gingiva and cultured with CsA. Total RNA was collected and expression of mRNAs examined using semi-quantitative RT-PCR analysis. Cathepsin B, D, and L expression in overgrown gingiva of the patient was examined by immunohistochemistry. RESULTS: Cathepsin D, L, and vascular endothelial growth factor (VEGF)165 mRNA were markedly suppressed in CsA-treated HGFs, whereas cathepsin B, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were not reduced. Next, the decrease of cathepsin B and L expression in enlarged gingiva was observed, whereas an increase of cathepsin D expression was observed. Clinically, the enlarged gingival lesions were fully resolved by performing oral infection control. CONCLUSIONS: Cathepsins regulation might be an important factor in the development of CsA-mediated gingival hyperplasia.
Asunto(s)
Catepsina B/genética , Catepsina D/genética , Catepsina L/genética , Ciclosporina/efectos adversos , Regulación de la Expresión Génica/efectos de los fármacos , Hiperplasia Gingival/metabolismo , Inmunosupresores/efectos adversos , Sarcoidosis/tratamiento farmacológico , Factor A de Crecimiento Endotelial Vascular/genética , Corticoesteroides/administración & dosificación , Corticoesteroides/uso terapéutico , Infecciones por Bacteroidaceae/complicaciones , Catepsina B/biosíntesis , Catepsina D/biosíntesis , Catepsina L/biosíntesis , Ciclosporina/administración & dosificación , Ciclosporina/farmacología , Ciclosporina/uso terapéutico , Raspado Dental , Quimioterapia Combinada , Inducción Enzimática/efectos de los fármacos , Femenino , Hiperplasia Gingival/inducido químicamente , Hiperplasia Gingival/etiología , Hiperplasia Gingival/prevención & control , Gingivitis/complicaciones , Gingivitis/microbiología , Gingivitis/terapia , Humanos , Huésped Inmunocomprometido , Inmunosupresores/administración & dosificación , Inmunosupresores/farmacología , Inmunosupresores/uso terapéutico , Metaloproteinasa 1 de la Matriz/biosíntesis , Metaloproteinasa 1 de la Matriz/genética , Persona de Mediana Edad , Higiene Bucal , Porphyromonas gingivalis/aislamiento & purificación , Sarcoidosis/complicaciones , Inhibidor Tisular de Metaloproteinasa-1/biosíntesis , Inhibidor Tisular de Metaloproteinasa-1/genética , Treponema denticola/aislamiento & purificación , Factor A de Crecimiento Endotelial Vascular/biosíntesisRESUMEN
In criminal investigations, usually it is necessary to identify whether blood spots found at crime scenes are from humans or not. Nowadays, immunohistochemical methods and DNA analysis are usually used for this purpose. However, such methods and DNA analysis are labor intensive and expensive, and require highly trained skilled technicians. Recently, the genome profiling method (GP method) was developed. However, its use as a human DNA analysis method has not been reported. In this report, an attempt was made to differentiate human blood samples from animal blood samples using the GP method for forensic purposes. DNA extracted from a rat, squirrel, cat, dog, cow, and antelope along with human blood samples were analyzed. Following cluster analysis the human samples clustered into a single group separate from the animal samples. Therefore, although the number of samples was small the results suggest that the GP method might enable us to differentiate human samples from various animal samples. It may become a powerful tool in the field of forensic science.