RESUMEN
Studies of the evolution of coastal lowlands since the Last Glacial Maximum (LGM) typically ignore radiocarbon data from sediment samples that have undergone reworking. However, these samples contain information on their sediment sources and the timing of their redeposition. We analyzed 738 radiocarbon dates obtained from shell and plant material in samples of post-LGM coastal sediment from north of Tokyo Bay, Japan. Of these samples, 245 (33%) were reworked. Furthermore, the percentage of reworked samples and their average age offsets increased with the depth of the water environment (terrestrial, 15% and 360 ± 250 years, respectively; intertidal, 26% and 470 ± 620 years; subtidal, 39% and 550 ± 630 years). Taking these radiocarbon samples as a proxy for clastic material, our results imply that channel erosion accounted for relatively little clastic removal in the terrestrial and intertidal environments over short timescales, whereas ~ 40% of clastics were removed by storm winnowing and transported in stepwise fashion to deeper water over longer timescales and ~ 60% in the subtidal environment were transported by floods directly from river mouths. These findings imply that radiocarbon ages from reworked samples can be used to quantify clastic recycling processes and their history in coastal areas.
RESUMEN
Anaerobic methane-oxidizing archaea (ANME) are known to play an important role in methane flux, especially in marine sediments. The 16S rRNA genes of ANME have been detected in terrestrial freshwater subsurfaces. However, it is unclear whether ANME are actively involved in methane oxidation in these environments. To address this issue, Holocene sediments in the subsurface of the Kanto Plain in Japan were collected for biogeochemical and molecular analysis. The potential activity of the anaerobic oxidation of methane (AOM) (0.38-3.54 nmol cm⻳ day⻹) was detected in sediment slurry incubation experiments with a (13) CH(4) tracer. Higher AOM activity was observed in low-salinity treatment compared with high-salinity condition (20), which supports the adaptation of ANME in freshwater habitats. The 16S rRNA sequence analysis clearly revealed the presence of a distinct subgroup of ANME-1, designated ANME-1a-FW. Phylogenetic analysis of the mcrA genes also implied the presence of the distinct subgroup in ANME-1. ANME-1a-FW was found to be the most dominant active group in the archaeal communities on the basis of 16S rRNA analysis (75.0-93.8% of total archaeal 16S rRNA clones). Sulfate-reducing bacteria previously known as the syntrophic bacterial partners of ANME-1 was not detected. Our results showed that ANME-1a-FW is adapted to freshwater habitats and is responsible for AOM in terrestrial freshwater subsurface environments.