RESUMEN
Dermatitis herpetiformis (DH) is characterized by deposition of IgA in the papillary dermis. However, indirect immunofluorescence is routinely negative, raising the question of the mechanism of formation of these immune deposits. Sárdy et al. (2002. J. Exp. Med. 195: 747-757) reported that transglutaminase-3 (TG3) colocalizes with the IgA. We sought to create such deposits using passive transfer of Ab to SCID mice bearing human skin grafts. IgG fraction of goat anti-TG3 or control IgG were administered i.p. to 20 mice. Separately, sera from seven DH patients and seven controls were injected intradermally. Biopsies were removed and processed for routine histology as well as direct immunofluorescence. All mice that received goat anti-TG3 produced papillary dermal immune deposits, and these deposits reacted with both rabbit anti-TG3 and DH patient sera. Three DH sera high in IgA anti-TG3 also produced deposits of granular IgA and TG3. We hypothesize that the IgA class anti-TG3 Abs are directly responsible for the immune deposits and that the TG3 is from human epidermis, as this is its only source in our model. These deposits seem to form over weeks in a process similar to an Ouchterlony immunodiffusion precipitate. This process of deposition explains the negative indirect immunofluorescence results with DH serum.
Asunto(s)
Dermatitis Herpetiforme/inmunología , Dermatitis Herpetiforme/patología , Modelos Animales de Enfermedad , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Trasplante de Piel/inmunología , Trasplante de Piel/patología , Transglutaminasas/inmunología , Animales , Complejo Antígeno-Anticuerpo/metabolismo , Sitios de Unión de Anticuerpos/inmunología , Tejido Conectivo/enzimología , Tejido Conectivo/inmunología , Reacciones Cruzadas/inmunología , Dermatitis Herpetiforme/enzimología , Dermis/inmunología , Dermis/metabolismo , Cabras , Humanos , Inmunización Pasiva/métodos , Inmunoglobulina A/administración & dosificación , Inmunoglobulina A/biosíntesis , Inmunoglobulina G/administración & dosificación , Inmunoglobulina G/biosíntesis , Inyecciones Intradérmicas , Masculino , Ratones , Ratones SCID , Conejos , Transglutaminasas/sangreRESUMEN
IgA is present in the skin in several dermatoses, including dermatitis herpetiformis, linear IgA bullous dermatosis, and Henoch-Schoenlein purpura. The neutrophilic infiltration in the area of the IgA deposition suggests that IgA is responsible for the associated inflammatory events. The mechanism for this process is unproven, but is likely to involve IgA-mediated neutrophil chemotaxis with inhibition of chemotaxis by dapsone. Elucidation of the mechanism of IgA-mediated inflammation will require an animal model. We have established a model for linear IgA bullous dermatosis as a prototype disease to be studied. IgA mouse monoclonal antibodies against a linear IgA bullous dermatosis antigen have been passively transferred to SCID mice with human skin grafts. This has produced neutrophil infiltration and basement membrane vesiculation in 4 of 12 mice tested. We conclude that an animal model for the pathogenesis of IgA dermatoses with IgA deposition and inflammation can be produced by passive transfer of mouse IgA antibodies against a linear IgA antigen.