RESUMEN
Three novel corynebacterial species were isolated from soil sampled at a paddock in Vilsendorf, North Rhine-Westphalia, Germany. The strains were coccoid or irregular rod-shaped, catalase-positive and pale white to yellow-orange in colour. By whole genome sequencing and comparison of the 16S rRNA genes as well as the whole genome structure, it was shown that all three strains represent novel species of the family Corynebacteriaceae, order Corynebacteriales, class Actinobacteria. This project describes the isolation, identification, sequencing, and phenotypic characterization of the three novel Corynebacterium species. We propose the names Corynebacterium kalinowskii sp. nov. (DSM 110639T=LMG 31801T), Corynebacterium comes sp. nov. (DSM 110640T=LMG 31802T), and Corynebacterium occultum sp. nov. (DSM 110642T=LMG 31803T).
Asunto(s)
Corynebacterium , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , Corynebacterium/clasificación , Corynebacterium/aislamiento & purificación , ADN Bacteriano/genética , Granjas , Ácidos Grasos/química , Alemania , Pigmentación , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNAsunto(s)
Registros Odontológicos/legislación & jurisprudencia , Procesos de Copia/legislación & jurisprudencia , Control de Formularios y Registros/legislación & jurisprudencia , Health Insurance Portability and Accountability Act/legislación & jurisprudencia , Humanos , Indiana , Derechos del Paciente/legislación & jurisprudencia , Medidas de Seguridad/legislación & jurisprudencia , Estados UnidosRESUMEN
The application of genome editing technologies, like CRISPR/Cas9 for industrially relevant microorganisms, is becoming increasingly important. Compared to other methods of genetic engineering the decisive factor is that CRISPR/Cas9 is relatively easy to apply and thus time and effort can be significantly reduced in organisms, which are otherwise genetically difficult to access. Because of its many advantages and opportunities, we adopted the CRISPR/Cas9 technology for Actinoplanes sp. SE50/110, the producer of the diabetes type II drug acarbose. The functionality of genome editing was successfully shown by the scarless and antibiotic marker-free deletion of the gene encoding the tyrosinase MelC, which catalyzes the formation of the dark pigment eumelanin in the wild type strain. The generated ΔmelC2 mutant of Actinoplanes sp. SE50/110 no longer produces this pigment and therefore the supernatant does not darken. Furthermore, it was shown that the plasmid containing the gene for the Cas9 protein was removed by increasing the temperature due to its temperature-sensitive replication. The precision of the intended mutation was proven and possible off-target effects caused by the genome editing system were ruled out by genome sequencing of several mutants.