Evaluation of infrared photography for latent bloodstain visualization and the influence of time.

The localization of latent blood traces at crime scenes is generally performed using fluorescent stains although infrared light has previously been recognized as an effective localization test for bloodstains as it is a non-destructive and non-contact technique. The goal of this study was to evaluate infrared photography for the detection of latent bloodstains on a large number of objects with different compositions frequently encountered at crime scenes. In this study we show that infrared light photography was able to detect bloodstains deposited on 71.7% of materials while bloodstains on 29.2% of materials could only be detected using infrared photography and not through visual photography. Bloodstain could be detected on most fabrics composed of 100% polyester, 100% cotton and 100% wool or a combination of these fibers with other types of fiber such as nylon or viscose. For other materials such as leather, tiles, wood, bricks, parquet, infrared did not improve the visibility of the bloodstains. Finally, the influence of the time of bloodstain deposition was tested over the period of 1 week and 1 month and did not reveal major differences compared to stains after 24 h drying time suggesting that time has little influence on the ability of infrared light and visual light to detect latent bloodstains.

Time since contact influences DNA profiling success of cartridges and fired cartridge casings.

Forensic DNA analysis of cartridges and fired cartridge casings remains challenging, possibly due to the heat and pressure generated during firing of the weapon as well as metal ions from the casings that have been suggested to initiate DNA degradation and inhibit PCR during the DNA profiling process. Even though recently developed DNA recovery protocols have shown to significantly improve DNA yields and DNA profile success rates no information is available on whether the time interval between contact and the DNA recovery process has an influence on these outcomes. In the current study 40 cartridges and 40 fired cartridge casings were left untreated for 24 h or 1 week after which the rinse-and-swab technique was used to collect DNA. Higher DNA yields and higher DNA profile success rates were obtained from cartridges compared to fired cartridge casings. The same general observation was made when cartridges and fired cartridge casings were processed after 24 h compared to after 1 week. In addition, DNA profiles suitable for comparison could still be generated from samples when real-time PCR quantification indicated DNA concentrations < 0.001 ng/µl, suggesting that quantification results may not be reliable when assessing the presence of DNA on such items. In conclusion, the results indicate that cartridges and fired cartridge casings should be processed for DNA profiling as soon as possible and that DNA quantification results should be interpreted with caution as DNA profiles suitable for comparison could be missed.

Influence of ink and smoke ATM security systems on dactyloscopy and subsequent DNA analysis after detonation.

Automated Teller Machine bombings are an increasing societal problem that are often committed using Improvised Explosive Devices. The evolution in IEDs and the negative consequences for society require new security measures to prevent these crimes. Ink staining and security smoke devices are added to cash cassettes, in order to protect ATMs and prevent ATM bombings. Traces found at crime scenes, such as fingerprints and DNA, can contribute to the identification of perpetrators. However, the effect of ink staining and security smoke devices on dactyloscopy and DNA profiling is still unknown. In the current study, we demonstrate that procedures using Citrus Cleaner or sulfosalicylic acid were successful in removing ink and security smoke deposited on plastic plates but did result in the massive loss of fingerprint information as only a low number (4%) of good quality fingerprints were recovered after smoke contamination. Secondly, security ink Sun Blue ES2, but not SICPA Green and Sun Blue ES1, had a significant impact on DNA profiling success. DNA concentrations obtained from blood spiked swabs decreased with increasing ink concentration resulting in a complete loss of genotype information with the addition of ≥10 µl Sun Blue ES2. No noticeable PCR inhibition or DNA degradation was detected during quantification, but a decreased efficiency of DNA extraction could not be excluded. Security smoke, on the other hand, does not seem to have a significant influence on DNA analysis. Precautions must therefore be taken in order to avoid contaminating DNA swabs with ink during sampling. Thirdly, only a single negative impression of a glove in ink and a single glove print were able to be visualized with white fingerprint powder on detonated cash cassettes. In conclusion, the detection of glove prints and fingerprints is limited and security ink, contrary to smoke, after detonation can have a negative influence on downstream DNA analysis procedures.

Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation.

Improvised Explosive Devices (IEDs) are weapons of modern times, used by terrorist groups and thereby causing substantial damage to communities. There is a widespread misconception that destructive conditions like heat, water or pressure destroy all forensic evidence. Moreover, the plausibility to detect identifiable fingermarks and DNA on components of IEDs is insufficiently known. Therefore, this study investigated the effects of neutralisation and explosion on latent fingerprints and touch DNA. In a majority of the cases, comparative fingermark- and DNA testing resulted in individualisation. In some cases, despite extremely low amounts of contact DNA detected after deployment of render-safe tools or detonation, full STR profiles could be constituted, even after applying fingerprint development techniques. This research shows that latent fingerprints and touch DNA on improvised explosives can be successfully detected after destructive conditions and possibly be linked to the perpetrators of such crimes. This individualising power offers perspectives to enhance forensic investigations of terrorism-related crimes.

Molecular footprint of drug-selective pressure in a human immunodeficiency virus transmission chain.

Known human immunodeficiency virus (HIV) transmission histories are invaluable models for investigating the evolutionary and transmission dynamics of the virus and to assess the accuracy of phylogenetic reconstructions. Here we have characterized an HIV-1 transmission chain consisting of nine infected patients, almost all of whom were treated with antiviral drugs at later stages of infection. Partial pol and env gp41 regions of the HIV genome were directly sequenced from plasma viral RNA for at least one sample from each patient. Phylogenetic analyses in pol using likelihood methods inferred an evolutionary history not fully compatible with the known transmission history. This could be attributed to parallel evolution of drug resistance mutations resulting in the incorrect clustering of multidrug-resistant virus. On the other hand, a fully compatible phylogenetic tree was reconstructed from the env sequences. We were able to identify and quantify the molecular footprint of drug-selective pressure in pol using maximum likelihood inference under different codon substitution models. An increased fixation rate of mutations in the HIV population of the multidrug-resistant patient was demonstrated using molecular clock modeling. We show that molecular evolutionary analyses, guided by a known transmission history, can reveal the presence of confounding factors like natural selection and caution should be taken when accurate descriptions of HIV evolution are required.