Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia.

Some species of the Baccharis genus have been shown to possess important biomedical properties, including cytotoxic activity. In this study, we examined the cytotoxic effect of methanol extract from Baccharis obtusifolia (Asteraceae) in cancer cell lines of prostate (PC-3), colon (RKO), astrocytoma (D-384), and breast (MCF-7). The methanolic extract displayed the largest substantial cytotoxic effect in lines of colon cancer (RKO) and cerebral astrocytoma (D-384). Chromatographic purification of the B. obtusifolia methanolic extract led to the isolation and identification of 5,4'-dihydroxy-7-methoxyflavone (1) and 5-hydroxy-7,4'-dimethoxyflavone (2) compounds of the flavonoid type.

Phytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves

Abstract Some species of the Clusia genus have been shown to have important biomedical properties, including the ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we examined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. & Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO), astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the most substantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induced a genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purification of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol, and hesperidin as active cytotoxic compounds in hexane extract, while β-amyrine was identified as an active cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further studies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cell survival.

Anticuerpos antipéptico citruliano en pacientes con artritis reumatoide. Hospital Vicente Corral Moscoso. Facultad de Ciencias Médicas. Cuenca. 2008 / Citruliano antipéptico antibodies in patients with rheumatoid arthritis. Vicente Corral Moscoso Hospital. Faculty of Medical Sciences. Cuenca

Se establecieron dos grupos de estudio, 30 pacientes con Artritis Reumatoide que cumplieron criterios diagnósticos del Colegio Americano de Reumatología y 30 controles que acudieron a consulta externa del Hospital Vicente Corral Moscoso en el período de enero a mayo de 2008. Se realizo el Factor Reumatoide y el Anti Péptido Cíclico Citrulinado y se confrontó los resultados. En el análisis estadístico se realizó la sensibilidad, especificidad, valores predictivos positivos y negativos. Resultados: la determinación de Anticuerpos Anti-pétido Cíclico Citrulinado generación 2 realizada por la técnica ELISA, presentó una sensibilidad del 53,3, una especificidad del 83,3, un valor predictivo positivo de 76,19y un valor predictivo vengativo de 64,10para la generación 3 la sensibilidad fue de 50, especificidad 86,67, valor predictivo positivo 78.95y valor predictivo negativo 63,41y el Factor Reumatoide isotipo IgM sensibilidad 53,3, especificidad 70, valor predictivo positivo 64y valor predictivo negativo 60. Conclusión: los Anti-pétido Cíclico Citrulinado presentaron una mayor especificidad que el Factor Reumatoide, sin embargo la sensibilidad obtenida fue similar en ambas pruebas comparadas.AU