Effects of high non-phytate phosphorus starter diet on subsequent growth performance and carcass characteristics of broiler chickens.

The trial was performed to investigate the effects of different concentrations of non-phytate phosphorus (nPP) in the starter and grower (with phytase inclusion) periods on carcass characteristics, organ weight and weekly variations of growth performance in the grower period. Seven hundred and twenty-day-old male broiler chickens were randomly assigned to 12 treatments in a completely randomized design. Chickens received two dietary treatments (4.5 g/kg and 6 g/kg nPP) in the starter (0-21 days) and six experimental diets (4 g/kg, 3.1 g/kg, 2.3 g/kg and 2.3 g/kg + 1000 FTU/Kg of feed phytase, 1.5 g/kg, 1.5 g/kg nPP + 1000 FTU/Kg of feed phytase) in the grower period (22-42 days). Results showed that phytase inclusion in the second and third weeks of grower period could increase feed intake significantly. Also, decrease in the concentrations of nPP to 1.5 g/kg caused to decline body weight gain markedly. Moreover, there is a significant difference between 4.5 g/kg and 6 + 4 g/kg nPP (starter+grower) and 1.5 g/kg nPP. Phytase inclusion increased carcass yield and declined liver weight significantly. Dietary treatment of 4.5 + 1.5 g/kg nPP enhanced heart and liver weight markedly. It is concluded that starter diets with increased concentration of nPP (6 g/kg nPP) had no beneficial effects on growth performance in the starter and grower period in the total (0-42 days). Also, it is possible to decrease nPP concentration of grower diets to 1.5 and 2.3 g/kg with and without phytase inclusion respectively.

Assessment of potential enhancing effects of a carbohydrase mixture on phytase efficacy in male broiler chicks fed phosphorus-deficient diets from 1 to 18 days of age.

A total of 540 one-day-old male broiler chicks were used to evaluate the potential enhancing effects of a mixture of 2 commercial carbohydrase preparations on phytase efficacy. A nonphytate phosphorus (nPP) deficient diet (0.15%) was supplemented with different levels of phytase (0X, 1X, 2X, and 3X of recommended level of 500 phytase units per kg of feed), individually or in combination with different levels of a mixture of 2 commercial carbohydrase enzymes [0X, 1X, and 2X of recommended level (X = 0.2 g per kg of feed; a mix with predominantly xylanase and ß-glucanase activity)] to determine their effects on performance and bone development. A standard response curve was developed using graded levels of nPP (0.15 to 0.45%) from dicalcium phosphate in a diet with no phytase supplementation to estimate the phosphorus equivalency of the different enzyme combinations. Each diet was fed to 6 replicate pens of 5 male chicks from 1 to 18 d of age. Phytase at normal usage levels of 500 phytase units per kg of feed was effective (P < 0.05) in improving BW, increasing feed intake, reducing mortality rate, and increasing toe and tibia ash without significantly influencing feed conversion. Addition of extra levels of phytase (2X and 3X) significantly (P < 0.05) improved toe and tibia ash without further affecting performance traits. The carbohydrase mixture failed to improve performance or enhance phytase efficacy. The phosphorus equivalency of phytase was dose dependent, with estimates of 0.100, 0.123, and 0.213% for 1X, 2X, and 3X supplementation levels of phytase, respectively, based on tibia ash. In conclusion, results indicate that increasing the level of phytase resulted in significant improvements in utilization of dietary nPP, whereas the carbohydrase preparation had no significant effects on performance or had limited effects in enhancing phytase ability in young broiler chicks.

Interactions between phytase and xylanase enzymes in male broiler chicks fed phosphorus-deficient diets from 1 to 18 days of age.

A total of 735 one-day-old male broiler chicks were used to evaluate the interactions between different levels of phytase and xylanase enzymes on performance and bone mineralization. Basal nonphytate P (nPP)-deficient diets (0.15%) were supplemented with different levels of phytase [0X, 1X, 2X, 3X, and 4X of recommended level (X = 500 phytase units per kg of feed)] alone or in combination with 3 levels of a xylanase preparation [0X, 1X, and 2X of recommended level (X = 0.1 g per kg of feed; a mixture with predominantly xylanase activity)]. A standard curve was developed using different levels of nPP (0.15 to 0.45%) to estimate the P equivalency of each enzyme combination. Treatments were replicated with 7 pens of 5 chicks each. The findings indicated that reducing dietary nPP level had a severely negative influence on bird performance and bone ash content. Results also showed that increasing levels of phytase significantly (P < 0.05) improved BW, feed intake, feed conversion ratio, mortality, and toe and tibia bone ash contents in a dose-dependent fashion. The P equivalency of phytase was also dose dependent, with estimates of 0.08, 0.11, 0.15, and 0.19 for 1X, 2X, 3X, and 4X supplementation levels of phytase, respectively. Xylanase preparation at 1X level failed to influence measured criterion; however, increasing the level of xylanase (2X) resulted in deteriorating BW and feed conversion ratio. The P equivalency of xylanase preparation at 1X and 2X was estimated as 0.010 and 0.014%. There were no interactions between phytase and xylanase preparation (P > 0.05). In conclusion, phytase was effective in improving bird performance and bone mineralization; however, xylanase supplementation failed to enhance phytase efficacy.

Influence of growth rate on the occurrence of white striping in broiler breast fillets.

White striping refers to the occurrence of different degrees of white striations on broiler breast fillets and thighs of larger broilers, yet little is known about its causes. Thus, the objective of the study was to estimate the occurrence of normal (NORM), moderate (MOD), and severe (SEV) degrees of white striping with respect to the growth rate of broilers and to compare their proximate composition without the confounding effect of diet. Straight-run 1-d-old chicks (n = 280) were randomly assigned to either a low- (LED) or high-energy (HED) diet (5 replicates of 28 birds/dietary treatment). Birds were processed at 54 d of age, and live weight, deboned fillet weight, and occurrence of white striping were recorded. As expected, birds fed the HED had lower (P < 0.05) feed conversion ratios than birds fed LED (2.08 vs. 2.28). Also, HED-fed birds had heavier P < 0.05) live and fillet weights when compared with the LED-fed birds. A greater (P < 0.05) percentage of breast fillets from LED-fed birds were scored NORM, whereas HED-fed birds produced a greater (P < 0.05) percentage of SEV fillets. Fillet weight and yield (percent of live weight) increased (P < 0.05) as the degree of white striping increased from NORM to SEV. Additionally, NORM fillets had greater (P < 0.05) lipid and lower (P < 0.05) protein content when compared with SEV fillets. Also, NORM fillets had greater (P < 0.05) percentages of SFA than SEV fillets; however, proportions of all monounsaturated fatty acids, as well as linoleic and linolenic acids, were greater (P < 0.05) in SEV than NORM fillets. These results suggest that an increased growth rate results in increased occurrence of higher degrees of white striping in broiler breast fillets, and the various degrees of white striping are associated with differences in chemical composition of breast fillets.

Effect of different levels of dietary vitamin E (DL-α-tocopherol acetate) on the occurrence of various degrees of white striping on broiler breast fillets.

White striping could be a potential reason for the rejection of raw breast fillets in the market. The condition is characterized grossly by the white striations occurring on the fillets showing myopathic changes on microscopic examination. Early research has shown similar lesions in the case of nutritional muscular dystrophy, which is a condition caused mainly by the deficiency of vitamin E in the diet. The present study was intended to evaluate the effect of different levels of dietary vitamin E (dl-α-tocopherol acetate) on the incidence of normal, moderate, and severe degrees of white striping, by modern description, on broiler breast fillets. Basal diets adequate for starter (0 to 18 d), grower (19 to 32 d), and finisher (33 to 49 d) age periods supplemented with 15, 50, 100, 200, and 400 IU of vitamin E/kg of feed were used in the study. Each of the 5 diet treatments were fed to 8 pens (53 birds each) of male broilers from a commercial strain. At 49 d, 5 birds were randomly selected from each pen (n = 40 birds/diet treatment) and were processed. Live weight, ready-to-cook weight, weight of the fillets, wings, tenders, legs, and the racks were obtained. The fillets were scored for the 3 degrees of white striping. There were no significant differences among the diet treatments with respect to the weight and carcass yield parameters. Furthermore, the diet treatments did not show any significant effect on the occurrence of normal, moderate, and severe degrees of white striping. However, fillet weight was the only parameter that had a significant effect on the occurrence of white striping. Higher degrees of white striping were seen associated with heavier fillets, which is in accordance with previous studies. Different levels of vitamin E levels used in the present study did not show any significant effect on the occurrence of 3 degrees of white striping. These results suggest that dietary vitamin E level is not associated with the modern condition of white striping in broiler breast meat.

Comparison of low dietary calcium and sodium sulfate for the potentiation of tetracycline antibiotics in broiler diets.

Three experiments were conducted to compare different methods of short-term potentiation of tetracycline antibiotics for broiler chicks. Reduction of dietary calcium and addition to sodium sulfate were compared in different combinations with various levels of chlortetracycline (CTC) and oxytetracycline (OTC). Reducing dietary calcium from 0.8 to 0.4% significantly increased serum levels of CTC and OTC. Adding sodium sulfate (1.25%) also increased serum levels; the majority of the response occurred at the higher calcium level. There was no benefit from adding sodium sulfate to the low-calcium diet. Neither body weight gain nor feed utilization were adversely affected during the 5-day test period.

Nutritional approaches to reducing phosphorus excretion by poultry.

Phosphorus is an essential mineral for growing poultry, and the consequences of a failure to provide for adequate quantities of this nutrient are physiologically and economically disastrous. Therefore, nutritionists provide a margin of safety for this mineral in their diets. However, because of growing concerns regarding the potential contribution of P in poultry excreta on eutrophication of surface waters, increasing pressure is being placed to limit the amount of excess P in diets and thus reduce fecal output. In order to significantly reduce fecal P while maintaining economic productivity, the nutritionist must establish and maintain an integrated program of activities, including an effective quality control program for incoming animal protein feeds, selection of P supplements of the highest biological value, use of phytase enzymes, and judicious selection of dietary P levels. Potential benefits of newer isomers of vitamin D and the commercial development of grains with high levels of nonphytate P offer promise in the future. Whatever measures are taken to increase the biological availability of the phytate-bound and nonphytate P portions of the diet, commensurate reductions in overall dietary P content must be made.

Effects of feeding extruded grain amaranth to laying hens.

Extruded grain amaranth (Amaranthus cruentus) was incorporated into corn-soybean meal layer rations at 0, 10, 20, or 30%. Diets were each fed to 20 Single Comb White Leghorn laying hens for 6 wk beginning at 65 wk of age. Data collected from the week prior to the test period were used as a covariate. Egg production was significantly higher and egg weights were significantly lower for birds fed the 10 and 20% inclusion levels of amaranth than for those fed the control diet. However, no differences were observed among the diets for daily egg mass. Significantly less feed was consumed at the 10% inclusion level of amaranth while achieving this higher production. Layers fed diets containing amaranth required significantly less feed to produce a dozen eggs or a gram of egg than those fed the control diet. No differences were observed among the treatments for shell strength, shell thickness, number or severity of blood spots, or Haugh units. Yolk color was negatively correlated with increased amaranth inclusion. No yolk or albumen discolorations were observed after 6 wk of storage. Extruded grain amaranth may be effectively used in layer rations without detrimentally altering production characteristics.

Effect of cage level (tier) on the performance of White Leghorn chickens.

Conflicting reports exist in the literature about the effects of cage level (tier) on laying hen performance. Results clearly indicate, under conditions of this study, that performance as measured by egg production, egg weight, and mortality significantly deteriorated in a linear fashion progressing from the bottom to the top in three and four tier cages. Causes may be related to fear associated with differences in light intensity or differences in exposure to visual stimuli between the tiers.

Estimation of the tryptophan requirement of male broiler chickens.

Three experiments were conducted to determine the response of the male broiler chick to tryptophan (TRP) supplementation. Chicks were fed from 7 to 18 or 7 to 20 days posthatch either a grain sorghum-soybean meal diet containing .16% TRP, supplemented with 0, .02, .04, .06, .09, or .11% L-TRP, or a corn-soybean meal positive control diet containing .25% TRP. Levels of TRP in all diets were confirmed by ion-exchange chromatography using an alkaline hydrolysis. The test diet contained 15.34% CP (N x 6.25) from intact ingredients and 20% CP (N x 6.25) when supplemented with other amino acids to 110% of National Research Council recommendations in 1984. No improvement in gain or feed efficiency was observed with chicks fed the grain sorghum-soybean meal diet at any level of TRP supplementation. Feed utilization but not weight gain of the chicks fed the TRP-supplemented test diets was significantly poorer than that of chicks fed the corn-soybean meal positive control diets. These results suggest that the TRP requirement of the young (0 to 21-day-old) broiler chicken is no greater than .16% of a diet containing 3,200 kcal ME/kg.

Use of organic acids in broiler diets.

Two feeding trials were carried out to determine the potential use of fumaric acid and calcium formate in diets for broiler chickens. In the first study using male broiler chicks fed to 21 days of age, fumaric acid was added at 0, .5, 1.0 and 1.5% and calcium formate was added at 0, .72, 1.48, 2.20, and 2.89%. All combinations of fumaric acid and calcium formate were evaluated in a 4 x 5 factorial arrangement of treatments. Addition of .5 or 1.0% fumaric acid significantly (P less than .05) improved body weights of broilers but did not influence feed utilization. Addition of calcium formate at levels greater than .72% significantly reduced both body weight and feed utilization. There was no significant interaction between levels of fumaric acid and calcium formate. In a second trial, male and female broiler chicks were grown to 49 days of age using diets containing 0, .5, 1.0, and 1.5% calcium formate. Addition of .5 or 1.0% calcium formate had no significant effect on weight gains or feed utilization. Addition of 1.5% calcium formate significantly reduced body weights of both males and females at 21 and 42 days. At 49 days of age, body weights of males fed 1.5% calcium formate did not differ from those of males fed the control diet, but were significantly less than those of males fed diets with .5 or 1.0% calcium formate. Female body weights at 49 days of age were significantly depressed by addition of 1.5% calcium formate.

Assessment of extruded grain amaranth as a feed ingredient for broilers. 1. Apparent metabolizable energy values.

Two trials were conducted to determine the apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) of extruded grain amaranth (Amaranthus cruentus). Procedures for these determinations were the total collection method (TCM) and the use of acid-insoluble ash (AIA) as a marker. The AME and AMEn values for the grain were determined both by regression analysis and by calculations based on differences from the basal diet. Based on standard errors of the predicted values and coefficients of determination of the regression equations, TCM produced more accurate results than did AIA. Using regression analysis, the predicted values from the TCM were 3,382 kcal/kg of AME and 3,267 kcal/kg of AMEn on an as-fed basis. When expressed on a dry-matter basis, these values were 3,646 and 3,522 kcal/kg for AME and AMEn, respectively. When the difference method was used to calculate the ME of the extruded grain on an as-fed basis, the values of 3,295 kcal/kg of AME and 3,164 kcal/kg of AMEn were obtained. When the results were expressed on a dry-matter basis, 3,556 kcal/kg of AME and 3,415 kcal/kg of AMEn were determined.

Assessment of extruded grain amaranth as a feed ingredient for broilers. 2. Apparent amino acid availability values.

Apparent amino acid availabilities (AAAA) were determined for grain amaranth in two experiments using broilers. Total collection method (TCM) and acid-insoluble ash (AIA), as a marker, were used for these determinations. Individual amino acid availabilities for the extruded grain were calculated both by regression analysis and by difference from the basal diet. From the linear regression equations, TCM produced more accurate results than did the AIA method, based on standard errors of the intercept and coefficient of determination. Predicted AAAA, based on linear regression equations, from the TCM were 92.4, 79.9, and 89.4% for methionine, lysine, and arginine, respectively. Using the TCM and calculation by difference, average AAAA on an as-fed basis were 85.0, 79.1, and 92.9% for methionine, lysine, and arginine, respectively. The AAAA of 11 other amino acids were also determined.

Performance and yields of broilers fed extruded grain amaranth and grown to market weight.

A trial was conducted to determine the effects of feeding extruded grain amaranth (Amaranthus cruentus) on performance of male broilers grown in batteries to 49 days of age. Practical corn-soy rations were supplemented with 0, 10, 20, 30, 40, or 50% amaranth and formulated to meet nutrient requirements for the starter (0 to 21 days), grower (21 to 42 days), and finisher (42 to 49 days) periods. Each diet was fed to six replicate pens of 10 male birds each. No differences were observed for feed utilization (P greater than .05), but at Day 49, birds fed 50% amaranth weighed significantly less and had significantly lighter dressed carcass weights than those fed the control diet. For dressing percentages, no significant differences among diets were observed. Dietary differences in fat pad weights were not detected, but significant differences between diets were observed for fat pad weights expressed as a percentage of the total carcass weight (% OTC). Significant and positive linear responses existed when liver weight, liver weight as % OTC, and fat pad weight as % OTC were regressed upon amaranth inclusion. No treatment differences were observed for heart or pancreas weights or for their weights as % OTC. Gizzard weight and gizzard weight as % OTC decreased linearly with amaranth inclusion. Giblet weights (heart + liver + gizzard) were significantly different among diets, but giblet weight appeared to be unrelated to amaranth level. Birds fed the diet with 50% amaranth had giblets of equal size to birds fed the control diet.(ABSTRACT TRUNCATED AT 250 WORDS)

Calculation and evaluation of energy: amino acid ratios for the egg-production type hen.

Published equations for estimating the daily energy needs of laying hens were combined with published equations for estimating the daily amino acid needs to derive estimated energy:amino acid ratios for laying hens at different stages of production and environmental temperatures. Two feeding trials were conducted using various percentages of the suggested ratios to determine their applicability. The results of these studies indicate that the calculated energy:amino acid ratios appeared to support production levels comparable with those of hens fed higher nutrient levels. The use of temperature-adjusted energy:amino acid ratios appears to be a valid and feasible means of formulating diets to meet the changing nutritional needs of laying hens.

Effect of dietary protein level and length of feeding on performance and abdominal fat content of broiler chickens.

Dietary CP levels influence carcass fat content of chickens but the length of time necessary to feed higher levels in order to increase carcass fat is not known. Studies were conducted to evaluate the effects of length of feeding of different levels of dietary CP on broiler performance and abdominal fat deposition. All diets were calculated to be isocaloric at 3,200 kcal ME/kg. Broilers fed 23% CP for 0 to 21 days, 20% CP for 21 to 42 days, and 18% CP for 42 to 49 or 42 to 56 days served as the control group in each of two trials. In the first trial, broilers were fed constant levels of CP from day-old to market age with CP levels ranging from 15 to 27% (amino acids adjusted in proportion to CP level). Other broilers were fed 23% CP for 0 to 21 days and then fed either 15, 17, 19, or 21% CP to market age. In the second trial, broilers were fed constant levels of CP (18, 20, or 23%) from day-old to market age. Feeding CP levels lower than those fed the control group had a more pronounced effect on males than on females, with the primary effects being reduced body weight, poorer feed utilization, and increased carcass fat content. Feeding CP levels higher than those fed the control group generally did not increase final body weight but generally improved feed utilization and decreased carcass fat content. The economics of improved feed utilization and reduced carcass fat must be considered in determining dietary CP levels to use at different ages for growing broilers.

Effects of different coccidiostats on performance of large white turkeys.

Two trials were conducted to evaluate the effects of feeding various anticoccidial products to turkeys to 8 wk and then growing to market age (16 wk for hens and 20 wk for toms). Anticoccidials evaluated in the first trial included amprolium at 187.5 mg/kg for 0 to 4 wk and 125 mg/kg for 4 to 8 wk; butynorate at 375 mg/kg for 0 to 8 wk; monensin at both 60 (MON-60) and 100 mg/kg for 0 to 8 wk; zoalene at 187.5 mg/kg for 0 to 4 wk and 125 mg/kg for 4 to 8 wk; and halofuginone at 3 mg/kg for 0 to 8 wk. In the second trial, MON-60 was replaced by a combination of sulfadimethoxine (62.5 mg/kg) plus ormetoprim (37.5 mg/kg) for 0 to 8 wk. In each trial each treatment was fed to four pens of 16 hens and four pens of 12 toms. Several of the anticoccidials significantly influenced the weight of both hens and toms by producing lower weights at the end of the 8-wk feeding period than birds in other treatments. However, after removal of the anticoccidials, compensatory gains were observed in almost every instance. Significant effects of previous anticoccidial feeding were noted on body weight of hens at 16 wk but not on weights of toms at 20 wk.

The influence of amprolium and butynorate on growth rate and feed utilization by large white turkeys.

Four feeding trials were conducted in which Large White turkeys were fed diets containing either amprolium or butynorate to 8 or 9 weeks of age to female and male turkeys, respectively. Diets fed during the study contained from 0 to 10% supplemental fat to provide a range of dietary energy levels. Poults were maintained on built-up wood shavings litter with no attempts to induce coccidiosis. Turkeys fed diets with amprolium did not grow as well or convert feed as efficiently as those fed butynorate at 8 or 9 weeks of age when the additives were withdrawn. However, at market weights or feed utilization between turkeys fed the two different anticoccidials.

Utilization of spent hen meal in diets for broiler chickens.

Studies were conducted to evaluate spent hen meal (SHM) produced in commercial rendering plants as a nutrient source in diets for broiler chickens. Utilizing previously determined nutrient composition values, including digestible amino acid and TMEn content, diets were formulated to include 0, 5, 10, and 15% of SHM from three different locations. In the first experiment, conducted in battery pens from 1 to 21 d posthatch, diets were formulated with digestible amino acid requirements set at 90, 95, or 100% of NRC (1994) total amino acid requirements. In the second experiment, conducted in floor pens from 1 to 49 d posthatch, diets were formulated with digestible amino acid requirements set at 95% of NRC (1994) total amino acid requirements. Samples of birds from the second experiment were processed to determine the possible influence of SHM inclusion on carcass yield. Results of the present studies indicate that SHM from commercial rendering facilities can be utilized in diets for growing broiler chickens provided adjustments are made in nutrient content and digestibility. When formulated on the basis of digestible amino acid content, levels of SHM up to 10% appear acceptable based upon body weight, feed conversion, bone ash, and carcass yield. Higher inclusion rates generally reduced performance. It is apparent that differences in nutritional quality may exist among products produced by different rendering facilities; however, evaluation of products to assess nutrient quality may be difficult under commercial conditions. As more information is generated regarding typical amino acid content and digestibility of rendered SHM, the product may be used with greater confidence in commercial diets.

Utilization of hydrolyzed sucrose polyester (olestra) in broiler diets.

Three experiments were conducted in which hydrolyzed olestra (HO) and hydrolyzed olestra manufacturing by-product (HBP) were compared with corn oil (CO) and a feed-grade hydrolyzed animal-vegetable fat blend (AVF) as fat supplements in diets for broilers. Various blends of HO and HBP with AVF and other fat sources were also evaluated. Results of these experiments indicate that HO or HBP may be used as energy sources in broiler diets. Use of these materials as the sole source of supplemental fat often reduced body weight gain and impaired feed utilization as compared with CO or AVF; however, when used as a component of a blended fat product typical of industry supplements, there was little if any adverse effect on live performance. Carcass fatty acid content was a reflection of dietary fat content. If the blend of fats containing HO or HBP contains a suitable ratio of unsaturated and saturated fatty acids, the product should be an acceptable source of supplemental energy in broiler diets.