[Establishment of rat integrated discrete multiple organ cell culture (IdMOC) model].

OBJECTIVE: To establish the integrated discrete multiple organ cell culture (IdMOC) system. METHODS: Rat primary cell of hepatocyte, nephrocyte, cardiomyocytes, alveolar macrophage, dermal fibroblasts were isolated by collagenase digestion, separation of bronchial lavage, two-step digestion method and cultured respectively, with monolayer culture. To establish the integrated discrete multiple organ cell culture (IdMOC) system, glass slides of five different cells were used to the same dish with 10% FBS DMEM medium cultured 7d, using MTT comparison primary cells cultured alone and cocultured when growth. RESULTS: Established rat hepatocytes, renal cell, cardiomyocyte, alveolar macrophages, dermal fibroblasts separation method was stable, cell separation survival rate was about 90.0%. Hepatocytes separation survival rate 90.3% ,renal cell separation survival rate 91.9%, cardiomyocyte separation survival rate 93.0% and beating rate indifference curve among 3d-15d, alveolar macrophages cell separation survival rate 90.8%, dermal fibroblasts cell separation survival rate 92.7%. Five primary cells multiple organ cells coculture showed cocultured cell growth proliferation well, cultured alone and cocultured cells growth curve basic coincide. CONCLUSION: Established rat multiple organ cell co-culture is successful.

Major achievements in relation to dyslexia in Chinese characters.

OBJECTIVE: To review the major achievements in dyslexia in Chinese characters, hoping to give some clues for future studies. DATA SOURCES: Both Chinese and English language literature search using PUBMED, and original articles published in main Chinese and international journals. STUDY SELECTION: After reviewing the literature, 54 articles were selected that specifically addressed the stated purpose. RESULTS: The results of studies about the subtypes, cerebral basis, reading processing model, event-related potential (ERP) and saccadic features between English and Chinese dyslexia are different. CONCLUSIONS: In the last ten years, great progress has been made in the study of dyslexia in Chinese characters. However, there are still many problems and shortcomings which need to be investigated.

[Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis].

OBJECTIVE: To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization. METHODS: Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis. RESULTS: The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma. CONCLUSION: The antigen IF is distributed in the intestine wall of A. cantonensis.