MicroRNA-181a-5p alleviates acute liver failure in mice by inhibiting HMGB1.

MicroRNAs (miRNAs) control liver diseases, but the role of microRNA-181a-5p in acute liver failure (ALF) is unclear. In this study, the ALF model was generated by injection of D-galactosamine (D-GalN) and lipopolysaccharide (LPS). The levels of miRNAs were assessed by microarray and qRT-PCR. The expression of caspase 3 was detected as the marker of cell apoptosis in ALF by immunohistochemistry and western blot. The targeting of microRNA-181a-5p on the high mobility group box 1 (HMGB1) was verified by dual luciferase assay. The impact of microRNA-181a-5p and HMGB1 was explored by flow cytometry. Results showed that microRNA-181a-5p was significantly down-regulated by D-GalN/LPS in vivo and in vitro, while the level of HMGB1 was up-regulated after the challenge. Furthermore, microRNA-181a-5p overexpression attenuated cell apoptosis in D-GalN/TNF-treated BNLCL2 cells. MicroRNA-181a-5p could directly target HMGB1 mRNA and repress its expressions, in further HMGB1 is involved in microRNA-181a-5p effect on cell apoptosis of ALF. In conclusion, these findings demonstrate that microRNA-181a-5p regulates hepatocyte apoptosis via HMGB1 in the development of ALF, which may provide potential therapeutic targets for ALF. However, the precise underlying mechanism that connects microRNA-181a-5p and HMGB1 remains to be explored.

Influences of temperature and salinity on holistic network performability of multi-AUV cooperative systems.

This study proposes holistic network performability as a reliability index of multiple autonomous underwater vehicle (AUV) (multi-AUV) cooperative systems and deduces the reliability of communication links. Temperature and salinity vary in different areas of the sea. The influence mechanism of temperature and salinity on holistic network performability is discussed. An example is provided to show the influences of temperature and salinity on holistic network performability. Corresponding trends of holistic network performability with the influences of temperature and salinity can be obtained. The trends show that the holistic network performability of multi-AUV cooperative systems exhibit ladder-like increases with temperature and salinity. In each ladder, the value of holistic network performability changes rapidly at high salinity or low temperature. The study on the change of the reliability of multi-AUV cooperative systems under different temperatures and salinities provides a theoretical basis for the reliability research of multi-AUV cooperative systems and provides reference for future practical application of multi-AUV cooperative systems and other network systems.