Organic media-based two-stage traditional and electrode-integrated tidal flow wetlands to treat landfill leachate: Influence of aeration strategy and plants.

Landfill leachate treatment employing normal and electrode-integrated constructed wetlands is difficult due to the presence of significant amounts of organic compounds, which frequently impede the progression of microbial-based aerobic pollutant removal pathways. As a result, this study examines the effect of supplementary air availability via intermittent and continuous aeration strategies in improving organic, nutrient, and coliform removals of the unplanted, planted (normal and electrode-integrated) two-stage tidal flow constructed wetlands designed to treat landfill leachate. The constructed wetlands were filled with coal and biochar media and planted with Canna indica. Mean chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), and coliform removal percentages of the externally aerated two-stage unplanted, only planted, planted-microbial fuel cell integrated constructed wetland systems ranged between 96 and 99%, 82 and 93%, 91 and 98%, 86 and 96%, respectively, throughout the experimental campaign. External aeration inhibited the development of a dominant anaerobic environment within the media of the wetland systems and improved overall pollutant removal. The electrode-integrated planted tidal flow wetlands produced better effluent quality than the unplanted or only planted tidal flow systems without electrode assistance. The first stages of the three wetland systems achieved an additional 5-7% COD, 7-12% TN, and 15-22% coliform removal during the continuous aeration period compared to the corresponding performance of the intermittent aeration phase. The pollutant removal performance of the second-stage wetlands decreased during the continuous aeration phase. The media composition supported electrochemically active and inactive microbial-based pollutant removal routes and the chemical adsorption of pollutants. Nitrogen and phosphorus accumulation percentage in plant tissues was low, i.e., 0.4-2.2% and 0.04-0.8%, respectively. During the continuous aeration period, the electrode-integrated tidal flow constructed wetlands achieved higher power density production, i.e., between 859 and 1432 mW (mW)/meter3(m3). This study demonstrates that external aeration might improve pollutant removal performance of the normal, electrodes integrated tidal flow-based constructed wetlands when employed for high organic-strength wastewater treatment such as landfill leachate.

Laboratory study of nitrification, denitrification and anammox processes in membrane bioreactors considering periodic aeration.

The possibility of using membrane bioreactors (MBRs) in simultaneous nitrification-anammox-denitrification (SNAD) by considering periodic aeration cycles was investigated. Two separate reactors were operated to investigate the effect of different anammox biomass in the presence of nitrifying and denitrifying biomass on the final nitrogen removal efficiency. The results illustrated that the reactor with higher anammox biomass was more robust to oxygen cycling. Around 98% Total Nitrogen (TN) and 83% Total Organic Carbon (TOC) removal efficiencies were observed by applying one hour aeration over a four-hour cycle. Decreasing the aeration time to 30, 15, and 2 min during a four-hour cycle affected the final TN removal efficiencies. However, the effect of decreasing aeration on the TN removal efficiencies in the reactor with higher anammox biomass was much lower compared to the regular reactor. The nitrous oxide (N2O) emission was a function of aeration as well, and was lower in the reactor with higher anammox biomass. The results of q-PCR analysis confirmed the simultaneous co-existence of nitrifiers, anammox, and denitrifiers in both of the reactors. To simulate the TN removal in these reactors as a function of the aeration time, a new model, based on first order reaction kinetics for both denitrification and anammox was developed and yielded a good agreement with the experimental observations.

Exploring the potential of graphite material in an unplanted electroactive wetland for the remediation of synthetic wastewater containing azo dye.

The current study was conducted to understand the sole role of graphite as a substrate material in a dual-chambered baffled electroactive wetland (EW) in the treatment of Methyl red dye-containing wastewater. The results obtained were compared with conventional gravel-based unplanted dual-chambered constructed wetlands (CW) at a lab scale. The highest dye decolorisation and COD removal efficiency achieved was 92.88 ± 1.6% and 95.78 ± 4.1%, respectively, in the electro-active wetland. Dissolved oxygen (DO) and pH conditions were appropriately maintained in both the microcosms because of separated aerobic and anaerobic chambers. UV-vis and gas chromatography-mass spectroscopy analysis revealed the production of by-products like 4-amino benzoic and N- N dimethyl phenyl-diamine of MR in microcosms and revealed further mineralisation of by-products in the aerobic zone of electroactive-wetland. Higher root growth of Cicer aerietinum and Vigna radiata was observed in the presence of effluents of baffled electroactive wetlands compared to constructed wetland, indicating a decrease in phytotoxicity. Metagenomic analysis revealed the abundance of potential microbes for MR and organic matter removal from phylum Proteobacteria, Firmicutes, Bacteroidetes, and Euryarchaeota. A batch adsorption study revealed a higher adsorption capability of graphite material in comparison to gravel. Hence, this study demonstrated that graphite is an appropriate substrate in electroactive wetland in facilitating microbial attachments and enhancing dye degradation, in addition to exhibiting superior adsorption quality.

Aluminium dross waste utilization for phosphate removal and recovery from aqueous environment: Operational feasibility development.

The need to minimize eutrophication in water bodies and the shortage of phosphate rock reserves has stimulated the search for sequestration and recovery of phosphate from alternative sources, including wastewater. In this study, aluminium dross (AD), a smelting industry waste/by-product, was converted to high-value material by encapsulation in calcium alginate (Ca-Alg) beads, viz. Ca-Alg-AD and utilized for adsorptive/uptake removal and phosphate recovery from an aqueous environment. Encapsulation of AD in alginate beads solves serious operational difficulties of using raw AD material directly due to density difference constraining efficient contact of AD with pollutants present in water and post-treatment recovery of AD material. The phosphate removal was evaluated in both batch and continuous flow operation modes. The batch adsorption study revealed 96.86% phosphate removal from 10 mg L-1 of initial phosphate concentration in 70 min of optimal contact time. Further, the phosphate removal potential of Ca-Alg-AD beads turned out to be independent of solution pH, with an average of 95.93 ± 1.40 % phosphate removal in the 2-9 pH range. The result reflects phosphate adsorption on Ca-Alg-AD beads following a second-order pseudo-kinetic model. Ca-Alg-AD beads-based adsorption followed Freundlich and Langmuir isotherm models. Further, a continuous packed bed column study revealed a total phosphate adsorption capacity of 1.089 mg g-1. The chemical composition, physical stability, and surface properties of Ca-Alg-AD beads were analyzed by means of state-of-the-art analytical techniques, such as Scanning Electron Microscopy-Energy Dispersive X-ray spectroscopy (SEM-EDX), Fourier Transform Infrared Spectroscopy (FTIR) and thermogravimetry/Differential Thermal Analysis (TG/DTA). These characterization techniques comprehend the mechanism and influence of surface properties and morphology on the phosphate adsorption behaviour, which induce the involvement of multiple mechanisms such as ligand complexation, ion exchange, and electrostatic attraction for phosphate adsorption on Ca-Alg-AD beads.

Nutrient removal in floating and vertical flow constructed wetlands using aluminium dross: An innovative approach to mitigate eutrophication.

On global scale, eutrophication is one of the most prevalent environmental threats to water quality, primarily caused by elevated concentration of nutrients in wastewater. This study utilizes aluminum dross (AD), an industrial waste, to create a value-added material by improving its operational feasibility and application for removing phosphate and ammonium from water. The operational challenges of AD such as its powdered nature and effective operation under only extreme pH conditions were addressed by immobilizing in calcium alginate to form calcium alginate aluminium dross (Ca-Alg-Al dross) beads. These Ca-Alg-Al dross beads were further tested for phosphate and ammonium removal from natural wastewater in two different aqueous environment systems: (i) vertical flow constructed wetlands (VF-CWs) followed by Ca-Alg-Al dross beads fixed bed system and (ii) Ca-Alg-Al dross beads mounted floating constructed wetlands (FCW) for remediating polluted lentic ecosystems. Our results show maximum phosphate and ammonium removal of 85 ± 0.41 % and 93.44 %, respectively, in VF-CWs followed by Ca-Alg-Al dross beads fixed bed system. The Ca-Alg-Al dross beads mounted FCW system achieved maximum phosphate removal of 79.18 ± 8.56 % and ammonium removal of 65.45 ± 21.04 %. Furthermore, the treated water from the FCW system was assessed for its potential to inhibit algal growth by artificially inoculating treated water with natural algae to simulate eutrophic conditions. Interestingly, treated water from the FCW system was found capable of arresting the algal growth. Besides, scanning electron microscopy with energy dispersive X-ray (SEM-EDX) and Fourier transform infrared (FTIR) spectroscopy confirmed the functional groups and surface properties and probable participation of multiple mechanisms including ion exchange, electrostatic attraction, and ligand complexation for phosphate and ammonium removal. Overall, these results offer a promising way to utilize AD for high-end applications in wastewater treatment.

Ozone disinfection of waterborne pathogens: A review of mechanisms, applications, and challenges.

Water serves as a critical vector for the transmission of pathogenic microorganisms, playing a pivotal role in the emergence and propagation of numerous diseases. Ozone (O3) disinfection technology offers promising potential for mitigating the spread of these pathogens in aquatic environments. However, previous studies have only focused on the inactivated effect of O3 on a single pathogenic microorganism, lacking a comprehensive comparative analysis of various influencing factors and different types of pathogens, while the cost-effectiveness of O3 technology has not been mentioned. This review synthesized the migration characteristics of various pathogenic microorganisms in water bodies and examined the properties, mechanisms, and influencing factors of O3 inactivation. It evaluated the efficacy of O3 against diverse pathogens, namely bacteria, viruses, protozoa, and fungi, and provided a comparative analysis of their sensitivities to O3. The formation and impact of harmful disinfection by-products (DBPs) during the O3 inactivation process were assessed, alongside an analysis of the cost-effectiveness of this method. Additionally, potential synergistic treatment processes involving O3 were proposed. Based on these findings, recommendations were made for optimizing the utilization of O3 in water inactivation in order to formulate better inactivation strategies in the post-pandemic eras.

Development of nature-based sustainable passive technologies for treating and disinfecting municipal wastewater: Experiences from constructed wetlands and slow sand filter.

There is an urgent need to develop low-cost technology for effective wastewater treatment and its further disinfection to the level that makes it economically useful. This work has designed and evaluated the various types of constructed wetlands (CWs) followed by a slow sand filter (SSF) for wastewater treatment and disinfection. The studied CWs were, CWs with gravels (CW-G), free water surface-CW (FWS-CWs), and CWs integrated microbial fuel cell (MFC) with granular graphite (CW-MFC-GG) planted with Canna indica plant species. These CWs were operated as secondary wastewater treatment technologies followed by SSF for disinfection purposes. The highest total coliform removal was observed in the combination of CW-MFC-GG-SSF which achieved a final concentration of 172 CFU/100 mL, whereas faecal coliform removal was 100 % with the combinations of CW-G-SSF and CW-MFC-GG-SSF, achieving 0 CFU/100 mL in the effluent. In contrast, FWS-SSF achieved the lowest total and faecal coliform removal attaining a final concentration of 542 CFU/100 mL and 240 CFU/100 mL, respectively. Furthermore, E. coli were detected as negative/absent in CW-G-SSF and CW-MFC-GG-SSF, while it was positive for FWS-SSF. In addition, the highest turbidity removal was achieved in CW-MFC-GG and SSF combination of 92.75 % from the municipal wastewater influent turbidity of 82.8 NTU. Furthermore, in terms of overall treatment performance of CW-G-SSF and CW-MFC-GG-SSF, these systems were able to treat 72.7 ± 5.5 % and 67.0 ± 2.4 % of COD and 92.3 % and 87.6 % of phosphate, respectively. Additionally, CW-MFC-GG also exhibited a power density of 85.71 mA/m3 and a current density of 25.71 mW/m3 with 700 Ω of internal resistance. Thus, CW-G and CW-MFC-GG followed by SSF could be a promising solution for enhanced disinfection and wastewater treatment.

Systematizing Microbial Bioplastic Production for Developing Sustainable Bioeconomy: Metabolic Nexus Modeling, Economic and Environmental Technologies Assessment.

The excessive usage of non-renewable resources to produce plastic commodities has incongruously influenced the environment's health. Especially in the times of COVID-19, the need for plastic-based health products has increased predominantly. Given the rise in global warming and greenhouse gas emissions, the lifecycle of plastic has been established to contribute to it significantly. Bioplastics such as polyhydroxy alkanoates, polylactic acid, etc. derived from renewable energy origin have been a magnificent alternative to conventional plastics and reconnoitered exclusively for combating the environmental footprint of petrochemical plastic. However, the economically reasonable and environmentally friendly procedure of microbial bioplastic production has been a hard nut to crack due to less scouted and inefficient process optimization and downstream processing methodologies. Thereby, meticulous employment of computational tools such as genome-scale metabolic modeling and flux balance analysis has been practiced in recent times to understand the effect of genomic and environmental perturbations on the phenotype of the microorganism. In-silico results not only aid us in determining the biorefinery abilities of the model microorganism but also curb our reliance on equipment, raw materials, and capital investment for optimizing the best conditions. Additionally, to accomplish sustainable large-scale production of microbial bioplastic in a circular bioeconomy, extraction, and refinement of bioplastic needs to be investigated extensively by practicing techno-economic analysis and life cycle assessment. This review put forth state-of-the-art know-how on the proficiency of these computational techniques in laying the foundation of an efficient bioplastic manufacturing blueprint, chiefly focusing on microbial polyhydroxy alkanoates (PHA) production and its efficacy in outplacing fossil based plastic products.

Performance assessment of normal and electrode-assisted floating wetlands: influence of input pollutant loads, surface area, and positioning of anode electrodes.

This study reports the design and development of microbial fuel cell (MFC) assisted floating wetlands and compares treatment removal performance with a normal (without electrodes) floating wetland. Both types of floating wetlands were planted with Phragmites plant and evaluated for real municipal wastewater treatment. The effective volume of each floating wetland was 0.5 m3. The floating wetlands were operated under variable hydraulic load rates, i.e., 20 and 60 mm/day. Mean 5-day biochemical oxygen demand (BOD5), chemical oxygen demand (COD), ammoniacal nitrogen (NH4-N), total nitrogen (TN), total phosphorus (TP), total suspended solids (TSS), and coliform removal percentages ranged between 71 and 96%, 72 and 94%, 62 and 86%, 58 and 75%, 82 and 97%, 64 and 92%, and 72 and 93%, respectively within the normal and electrode-assisted MFC integrated floating wetlands. The electrode-integrated floating wetlands showed better pollutant removal performance than the normal system under unstable input pollutant loading conditions. Nitrogen and organic matter removals were achieved through both electrochemically active and inactive microbial removal routes. Physical separation processes, such as filtration and sedimentation, contributed to phosphorus, solids, and coliform removal. Plant uptake contributed to micro-scale nitrogen (≤ 1%) and phosphorus (≤ 0.1%) removal. Increment of hydraulic/pollutant load improved organic removal but decreased nutrient removal performance of the normal, electrode-integrated floating wetlands. The electrode-integrated floating wetlands produced power densities ranging between 0.7 and 1.4 mW/m3, and 0.2 and 2.3 mW/m3 during lower, upper input loading ranges, respectively. Bioenergy production of the electrode-integrated floating wetlands varied within the two operational periods due to a wider range of electrochemically inactive microbial populations in real wastewater that interfered with electrochemical organic matter oxidation. The positioning difference of the anode electrodes was a significant factor that improved pollutant removal within the electrode-integrated floating wetlands compared to the other variable, i.e., anode electrodes surface area.

Binder-free NiO/MnO2 coated carbon based anodes for simultaneous norfloxacin removal, wastewater treatment and power generation in dual-chamber microbial fuel cell.

Norfloxacin (NFX) is a commonly consumed synthetic antibiotic drug to cure many adverse infectious diseases of humans worldwide, but their presence in almost all aquatic environments has grown into severe global health concerns. In this study, the power performance of dual-chamber microbial fuel cells (MFCs) with two different types of base anodes (graphite felt and activated carbon cloth) were tested with a coating of NiO/MnO2 for removal of NFX in wastewater. As transition metal oxides have excellent electrochemical stability and a higher specific capacitance, their application in MFC for antibiotic removal and wastewater treatment would be an interesting study. Four different NFX concentrations were studied in two different base material with a coating of NiO/MnO2. Coating was done with 2 step hydro solvothermal method and modified anode surface was characterized by XRD and XPS analyses. Extracellular electron transfer between microorganisms and the modified anode improved significantly as a consequence of reduced internal resistance and a more biocompatible surface as measured by Electroscopy Impedance Spectroscopy (EIS) and polarization curves. NiO/MnO2 coated graphite felt performed 1.2 fold better than the control plain graphite felt. Similar results were found for activated carbon cloth (ACC). Modified ACC performed 1.3 fold better than the control plain ACC.

Ozone based inactivation and disinfection in the pandemic time and beyond: Taking forward what has been learned and best practice.

The large-scale global COVID-19 has a profound impact on human society. Timely and effectively blocking the virus spread is the key to controlling the pandemic growth. Ozone-based inactivation and disinfection techniques have been shown to effectively kill SARS-CoV-2 in water, aerosols and on solid surface. However, the lack of an unified information and discussion on ozone-based inactivation and disinfection in current and previous pandemics and the absence of consensus on the main mechanisms by which ozone-based inactivation of pandemic causing viruses have hindered the possibility of establishing a common basis for identifying best practices in the utilization of ozone technology. This article reviews the research status of ozone (O3) disinfection on pandemic viruses (especially SARS-CoV-2). Taking sterilization kinetics as the starting point while followed by distinguishing the pandemic viruses by enveloped and non-enveloped viruses, this review focuses on analyzing the scope of application of the sterilization model and the influencing factors from the experimental studies and data induction. It is expected that the review could provide an useful reference for the safe and effective O3 utilization of SARS-CoV-2 inactivation in the post-pandemic era.

A glance of coupled water and wastewater treatment systems based on microbial fuel cells.

Microbial fuel cell (MFC) is a variant of the bioelectrochemical system that uses microorganisms as biocatalysts to generate bioenergy by oxidizing organic matter. Due to its two-prong feature of simultaneously treating wastewater and generating electricity, it has drawn extensive interest by scientific communities around the world. However, the pollution purifying capacity and power production of MFC at the laboratory scale have tended to remain steady, and there have been no reports of a performance breakthrough. In recent years, research related to MFC has demonstrated a new trend, namely the coupling of MFC with other wastewater treatment technologies to create a 1 + 1 > 2 impact. MFC-based coupling/hybrid technologies such as sediment MFC (SMFC), constructed wetland MFC (CW-MFC), membrane bioreactor MFC (MBR-MFC), microbial desalination cell (MDC), and MFC coupled nutrient recovery technology, etc. have been increasingly studied. Therefore, this review aims to overview these already-emerging MFC coupling technologies and explores their development trends and challenges to serve as a guide for determining priority research topics in this area. Among these MFC-based coupling/hybrid technologies, literature seems to support that CW-MFC is a good example of integrated MFC technology where CWs are already employed at the field level for wastewater treatment application. MFC-Electroflocculation and MBR-MFCs are typical emerged hybrid systems to own promising potential. However, scalability and practical application potential of these integrated technologies are the challenge towards their reality except for ideal performance in small scale trials.

Influence of aeration, plants, electrodes, and pollutant loads on treatment performance of constructed wetlands: A comprehensive study with septage.

This study reports the performance of non-aerated and aerated unplanted, planted, microbial fuel cell planted wetlands for stabilizing septage and treating the drained wastewater. The wetland systems of this study were dosed with septage for a relatively shorter period, i.e., 20 weeks, followed by 60 days of sludge drying period. The sludge loading rates across the constructed wetlands ranged between 259 and 624 kg total solids (TS)/m2 per year. Organic matter, nitrogen, and phosphorus concentration of the residual sludge ranged between 8512 and 66,374 mg/kg, 12,950 and 14,050 mg/kg, 4979 and 9129 mg/kg, respectively. The presence of plants, electrode, and aeration improved sludge dewatering and decreased the organic matter and nutrient concentration of the residual sludge. The heavy metals (Cd, Cr, Cu, Fe, Pb, Mn, Ni, and Zn) concentration of the residual sludge fulfilled the guidelines for agricultural reuse in Bangladesh. Chemical oxygen demand (COD), ammoniacal nitrogen (NH4-N), total nitrogen (TN), total phosphorus (TP), and coliform removal percentages from the drained wastewater ranged between 91 and 93 %, 88 and 98 %, 90 and 99 %, 92 and 100 %, and 75 and 90 %, respectively. NH4-N removal from the drained wastewater depended upon aeration. The sludge treatment wetlands achieved metals removal percentages (from the drained wastewater) ranging between 90 and 99 %. Physicochemical and microbial routes in accumulated sludge, rhizosphere, and media contributed to pollutants removal. Input load and organic removal increment (from the drained wastewater) were positively correlated; nutrient removal showed a contradictory trend. The non-aerated and aerated microbial fuel cell planted wetlands produced maximum power densities ranging between 66 and 3417 mW/m3. Because of the shorter experimental duration, this study revealed preliminary but new information on the macro and micro pollutants removal pathways in septage sludge wetlands (with and without electrode) that could be utilized to design pilot or full-scale systems.

The race between classical microbial fuel cells, sediment-microbial fuel cells, plant-microbial fuel cells, and constructed wetlands-microbial fuel cells: Applications and technology readiness level.

Microbial fuel cell (MFC) is an interesting technology capable of converting the chemical energy stored in organics to electricity. It has raised high hopes among researchers and end users as the world continues to face climate change, water, energy, and land crisis. This review aims to discuss the journey of continuously progressing MFC technology from the lab to the field so far. It evaluates the historical development of MFC, and the emergence of different variants of MFC or MFC-associated other technologies such as sediment-microbial fuel cell (S-MFC), plant-microbial fuel cell (P-MFC), and integrated constructed wetlands-microbial fuel cell (CW-MFC). This review has assessed primary applications and challenges to overcome existing limitations for commercialization of these technologies. In addition, it further illustrates the design and potential applications of S-MFC, P-MFC, and CW-MFC. Lastly, the maturity and readiness of MFC, S-MFC, P-MFC, and CW-MFC for real-world implementation were assessed by multicriteria-based assessment. Wastewater treatment efficiency, bioelectricity generation efficiency, energy demand, cost investment, and scale-up potential were mainly considered as key criteria. Other sustainability criteria, such as life cycle and environmental impact assessments were also evaluated.

Development of electrodes integrated hybrid constructed wetlands using organic, construction, and rejected materials as filter media: Landfill leachate treatment.

This study developed microbial fuel cell (MFC)-based hybrid constructed wetland systems using different filter media, i.e., organic (biochar), construction (sand), and rejected (iron particle, concrete particle, and stone dust) materials, and evaluated the performance of the developed systems for treating landfill leachate. The mean ammonium nitrogen (NH4-N), total nitrogen (TN), total phosphorus (TP), biochemical oxygen demand (BOD), chemical oxygen demand (COD) removal percentages within the hybrid systems ranged between 91 and 98%, 90 and 98%, 97 and 99%, 88 and 93%, 93 and 97%, respectively, despite higher pollutants concentration in leachate wastewater. The aerobic environment in the cathode compartment (due to intermittent load) and free-draining of wastewater (from cathode to anode compartment) supported electrochemically inactive, active pollutants removal in the electrodes integrated first stage vertical flow (VF) wetlands. The second stage electrodes integrated horizontal flow (HF) wetlands supported electrochemical-based organic removal and nitrification because of efficient organic removal in the previous VF wetland stages. Nitrogen, phosphorus accumulation percentages in plant tissues ranged between 0.3 and 7%, 0.4 and 14%, respectively. Nutrient removal was achieved through chemical and microbial routes. The biochar-packed VF wetland produced a maximum power density of 20.6 mW/m2. The coexistence of unsaturated, saturated media in the partially saturated HF wetland maintained the required environmental gradient between the electrodes and improved operational performance.

A comparative landfill leachate treatment performance in normal and electrodes integrated hybrid constructed wetlands under unstable pollutant loadings.

This study provides a comparative pollutant removal performance assessment between organic or construction materials-based four hybrid wetland systems that received landfill leachate. The hybrid systems included vertical flow (VF) followed by horizontal flow (HF)-based unplanted and planted systems, and planted electrodes incorporated microbial fuel cell (MFC) integrated hybrid wetlands systems. All the systems were run in free-draining mode. Overall mean chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) removal percentage of the hybrid systems ranged between 81 and 99%, 82 and 96%, 74 and 99%, respectively, under unstable input pollutant loading conditions. Additionally, up to 27% organic and up to 14% nitrogen removal improvement was observed in electrodes integrated free-draining VF wetlands. Free-draining and additional oxygen availability from atmospheric diffusion, rootzone improved the removal performance of MFC-based VF wetlands. Input load increment decreased organic, nutrient removals in second stage HF units due to saturated media. The chemical composition of the employed media supported biotic, abiotic organic, nutrient removal pathways. Nutrient accumulation percentage in plants tissue was very low, i.e., ≤3%. Bioenergy production across the MFC-based VF-HF wetlands decreased with input pollutant load increment. The single anode electrode-based VF wetland achieved maximum power density production, i.e., 294 mW/m2.. The electrodes integrated hybrid systems achieved comparatively stable removal performance despite input pollutant/hydraulic load variation.

Towards a more labor-saving way in microbial ammonium oxidation: A review on complete ammonia oxidization (comammox).

In the Anthropocene, nitrogen pollution is becoming an increasing challenge for both mankind and the Earth system. Microbial nitrogen cycling begins with aerobic nitrification, which is also the key rate-limiting step. For over a century, it has been accepted that nitrification occurs sequentially involving ammonia oxidation, which produces nitrite followed by nitrite oxidation, generating nitrate. This perception was changed by the discovery of comammox Nitrospira bacteria and their metabolic pathway. In addition, this also provided us with new knowledge concerning the complex nitrogen cycle network. In the comammox process, ammonia can be completely oxidized to nitrate in one cell via the subsequent activity of the enzyme complexes, ammonia monooxygenase, hydroxylamine dehydrogenase, and nitrite oxidodreductase. Over the past five years, research on comammox made great progress. However, there still exist a lot of questions, including how much does comammox contribute to nitrification? How large is the diversity and are there new strains to be discovered? Do comammox bacteria produce the greenhouse gas N2O, and how or to which extent may they contribute to global climate change? The above four aspects are of great significance on the farmland nitrogen management, aquatic environment restoration, and mitigation of global climate change. As large number of comammox bacteria and pathways have been detected in various terrestrial and aquatic ecosystems, indicating that the comammox process may exert an important role in the global nitrogen cycle.

An algal assisted constructed wetland-microbial fuel cell integrated with sand filter for efficient wastewater treatment and electricity production.

In this study we report on a three-stage design of algal assisted constructed wetland -microbial fuel cell incorporated sand filter for efficient treatment of wastewater and bioelectricity generation, simulating a core of it. It aims for the complete removal of organic matter and other inorganic pollutants such as NO3-, NH4+ and PO43-. The conductive material equipped anode region of constructed wetland (CW) performed better for organic removal, and the system efficiently removed COD up to 96.37 ± 2.6%. The algal biomass passively aerated the cathode region and resulted in efficient removal of 85.14 ± 10.73% of NH4+ and 69.03 ± 10.14% of PO43-. The additional sand filter provided anaerobic situation favorable for removal of NO3- up to 68.41 ± 7.63%. The maximum power and current densities achieved in the system were 33.14 mW m-3 and 235.0 mA m-3, respectively.

A comprehensive review on emerging constructed wetland coupled microbial fuel cell technology: Potential applications and challenges.

Constructed wetlands (CWs) integrated with bioelectrochemical systems (BESs) are being intensively researched with the names like constructed wetland-microbial fuel cell (CW-MFC), electro-wetlands, electroactive wetlands, and microbial electrochemical technologies-based constructed wetland since the last decade. The implantation of BES in CW facilitates the tuning of redox activities and electron flow balance in aerobic and anaerobic zones in the CW bed matrix, thereby alleviating the limitation associated with electron acceptor availability and increasing its operational controllability. The benefits of CW-MFC include high treatment efficiency, electricity generation, and recalcitrant pollutant abatement. This article presents CW-MFC technology's journey since its emergence to date, encompassing the research done so far, including the basic principle and functioning, bio-electrocatalysts as its machinery, influential factors for microbial interactions, and operational parameters controlling different processes. A few key challenges and potential applications are also discussed for the CW-MFC systems.

Interrelation between sulphur and conductive materials and its impact on ammonium and organic pollutants removal in electroactive wetlands.

This investigation is the first of its kind to evaluate the interrelation of sulphate (SO42-) with conductive materials as well as their individual and synergetic effects on the removal of ammonium and organic pollutants in electroactive wetlands, also known as constructed wetland (CW) - microbial fuel cell (MFC). The role of MFC components in CW was investigated to treat the sulphate containing wastewater under a long-term operation without any toxicity build-up in the system. A comparative study was also performed between CW-MFC and CW, where sulphate containing wastewater (S-replete) and without sulphate wastewater (S-deplete) was assessed. The S-replete showed high NH4+ removal than the S-deplete, and the requesnce of removal was: CW-MFC-replete>CW-MFC-deplete>CW-replete>CW-deplete. The chemical oxygen demand (COD) removal was high in the case of CW-MFC-replete, and the sequence of removal was CW-MFC-replete>CW-MFC-deplete>CW-deplete>CW-replete. X-ray photon spectroscopic study indicates 0.84% sulphur accumulation in CW-MFC-replete and 2.49% in CW-replete, indicating high sulphur precipitation in CW without the MFC component. The high relative abundance of class Deltaproteobacteria (7.3%) in CW-MFC-replete along with increased microbial diversity (Shannon index=3.5) rationalise the symbiosis of sulphate reducing/oxidising microbes and its impact on the treatment performance and electrochemical activity.