A Dual Functional Drug Delivery System that Combines Photothermal Therapy and Immunotherapy to Treat Tumors.

Cancer is one of the main diseases threatening human health. Immunotherapy, in which cancer is treated by activating immune cells and inducing the body's immune response, has rapidly developed. Photothermal therapy (PTT), a new treatment method that ablates tumors by light irradiation, has attracted great attention for its good therapeutic effect and low toxic side effects. In the present study, we combined photothermal and immunotherapy to design a novel nanoparticle delivery system by loading indoleamine 2,3-dioxygenase (IDO) inhibitors and toll-like receptor (TLR) agonists into polydopamine (PDA) nanoparticles coated with polyethylene imine (PEI). This delivery system has the advantages of high homogeneity, good stability, excellent biocompatibility, and low toxicity. In vitro antitumor studies showed that the system effectively inhibited the proliferation of mouse breast carcinoma cells and induced cell apoptosis. From the in vivo studies, we found that the system inhibited the growth of mouse breast carcinoma, facilitated the maturation of antigen-presenting cells, promoted T lymphocyte differentiation, and induced the body's immune response. The present study developed a dual functional drug delivery system combining photothermal therapy and immunotherapy to efficiently improve antitumor therapy with potential clinical application.

A new Ti-based IMAC nanohybrid with high hydrophilicity and enhanced absorption capacity for the selective enrichment of phosphopeptides.

Ti-based immobilized metal affinity chromatography (IMAC) nanomaterial has shown high potential in phosphoproteome mass-spectrometric (MS) analysis. However, the limited surface area and poor solubility will greatly restrict its use in phosphoproteome research. To overcome these two key drawbacks, a novel Ti-based IMAC nanomaterial was prepared by Ti-bonded ß-cyclodextrin (ß-CD) anchored on the surface of carbon nanotubes (CNTs) (denoted as COOH-CNTs-CD-Ti) and successfully applied as a biofunctional adsorbent for selectively enriching trace phosphopeptides. In the selective enrichment process, CNTs provided greater surface area for the absorption of phosphopeptides, while ß-CD also offered a greater opportunity for the interaction between phosphopeptides and Ti4+. COOH-CNTs-CD-Ti with the aforementioned properities exhibited higher selectivity for phosphopeptides from the standard protein digests, the tryptic digests of nonfat milk and human serum, showing a great selective enrichment capability towards complex biological samples.