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1.
Theor Appl Genet ; 137(1): 28, 2024 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-38252297

RESUMEN

KEY MESSAGE: We developed an array of Zea-Tripsacum tri-hybrid allopolyploids with multiple ploidies. We unveiled that changes in genome dosage due to the chromosomes pyramiding and shuffling of three species effects karyotypic heterogeneity, reproductive diversity, and phenotypic variation in Zea-Tripsacum allopolyploids. Polyploidy, or whole genome duplication, has played a major role in evolution and speciation. The genomic consequences of polyploidy have been extensively studied in many plants; however, the extent of chromosomal variation, genome dosage, phenotypic diversity, and heterosis in allopolyploids derived from multiple species remains largely unknown. To address this question, we synthesized an allohexaploid involving Zea mays, Tripsacum dactyloides, and Z. perennis by chromosomal pyramiding. Subsequently, an allooctoploid and an allopentaploid were obtained by hybridization of the allohexaploid with Z. perennis. Moreover, we constructed three populations with different ploidy by chromosomal shuffling (allopentaploid × Z. perennis, allohexaploid × Z. perennis, and allooctoploid × Z. perennis). We have observed 3 types of sexual reproductive modes and 2 types of asexual reproduction modes in the tri-species hybrids, including 2n gamete fusion (2n + n), haploid gamete fusion (n + n), polyspermy fertilization (n + n + n) or 2n gamete fusion (n + 2n), haploid gametophyte apomixis, and asexual reproduction. The tri-hybrids library presents extremely rich karyotype heterogeneity. Chromosomal compensation appears to exist between maize and Z. perennis. A rise in the ploidy of the trihybrids was linked to a higher frequency of chromosomal translocation. Variation in the degree of phenotypic diversity observed in different segregating populations suggested that genome dosage effects phenotypic manifestation. These findings not only broaden our understanding of the mechanisms of polyploid formation and reproductive diversity but also provide a novel insight into genome pyramiding and shuffling driven genome dosage effects and phenotypic diversity.


Asunto(s)
Poaceae , Zea mays , Zea mays/genética , Cariotipo , Haploidia , Poliploidía , Variación Biológica Poblacional
2.
Environ Res ; 194: 110622, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33359699

RESUMEN

Halogenated methyl sulfonic acid (HMSAs) is a new type of disinfection by-product recently reported, and there are few relevant studies, so its source and harm are still unclear. This study mainly discusses the source of halogenated methyl sulfonic acid from the macroscopic level and evaluates the harm to human health. This study clarified that chlorine disinfection is one of the main ways of generating halogenated methyl sulfonic acids (HMSAs) in drinking water. The macroscopic properties of HMSA precursors were analyzed through liquid chromatograph-mass spectrometry (LC-MS/MS), 3D fluorescence, and Fourier transform infrared spectrometry. The results showed that polar and positively charged organic compounds with molecular weights of >3 KDa or <0.5 KDa can readily generate HMSAs. By analyzing the main components of natural organic compounds in water and comparing them with the characteristics of organic compounds such as dimethyl sulfoxide and ethyl mercaptan, it meant that natural organic compounds are easily degraded. Humic and fulvic acids contribute the most to HMSA generation. This study lasted for three years and collected water samples from 102 waterworks in 24 cities in China, and the existence of HMSAs in drinking water in different cities was analyzed. The potential health risks associated with HMSAs were used by the United States Environmental Protection Agency (U.S. EPA) health risk assessment model. The average and maximum concentrations of HMSAs in tap water from key cities in China were taken as evaluation objects. The results showed that the carcinogenic risk coefficient (R) value of both concentrations was far lower than the lowest acceptable carcinogenic risk (1.0 × 10-6) provided by U.S. EPA. Therefore, HMSAs in drinking water in China currently pose no human health risks.


Asunto(s)
Agua Potable , Contaminantes Químicos del Agua , Purificación del Agua , China , Cromatografía Liquida , Ciudades , Desinfección , Humanos , Ácidos Sulfónicos , Espectrometría de Masas en Tándem , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
3.
BMC Plant Biol ; 20(1): 247, 2020 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-32487142

RESUMEN

BACKGROUND: Tuber color is an important trait for Helianthus tuberosus L. (Jerusalem artichoke). Usually, purple tubers with high anthocyanin content are more nutritious than white tuber. But, the molecular mechanism underlying it is unknown. RESULTS: In the current study, high-throughput RNA-sequencing was used to compare the transcriptomes between plants with tubers with red or white epidermis. Compared with the white-skinned tubers of cultivar QY3, anthocyanin biosynthesis structural genes had greater expression in the red-skinned tubers of cultivar QY1, indicating that the anthocyanin biosynthesis pathway was activated in 'QY1'; quantitative PCR confirmed this difference in expression. HtMYB2 (Unigene44371_All) was the only MYB transcription factor, homologous to the MYB transcription factor regulating anthocyanin biosynthesis, expressed in the red tuber epidermis of 'QY1'. The anthocyanin concentration in the root, stem, leaf, flower, and tuber epidermis of 'QY1' was higher than in 'QY3', especially tuber epidermis. Correspondingly, HtMYB2 had greater expression in these tissues of 'QY1' than in 'QY3'. The expression of HtMYB2 was associated with anthocyanin accumulation in the different tissues. Overexpression of HtMYB2 activated the anthocyanin biosynthesis pathway, accumulating the pigment in leaves of transgenic tobacco, supporting the model that HtMYB2 regulated anthocyanin biosynthesis. Further experiments found that HtMYB2 had the same coding sequence and genomic sequence in 'QY1' and 'QY3', but that there were several single nucleotide polymorphisms and one insertion-deletion (indel) mutation of 21 nucleotides in the promoter region between the two alleles. The deletion of three nucleotides "AAA" made the promoter of 'QY1' predicted to contain one more possible promoter region. A specific primer, based on the indel, could differentiate between cultivars with red or white tuber epidermis. The genetic variation in HtMYB2 was associated with the tuber skin color in a natural population. CONCLUSIONS: RNA-seq can successfully isolate the candidate gene (HTMYB2) controlling anthocyanin biosynthesis in purple epidermis of Jerusalem artichoke tuber. HTMYB2 can regulate anthocyanin biosynthesis in plants and is closely related to the formation of purple phenotype in tubers. This study should be useful in understanding the genetic mechanism underlying different tuber skin colors and in breeding new H. tuberosus cultivars with different tuber skin colors.


Asunto(s)
Antocianinas/biosíntesis , Genes de Plantas/genética , Helianthus/genética , Proteínas de Plantas/genética , Factores de Transcripción/genética , Perfilación de la Expresión Génica , Genes de Plantas/fisiología , Helianthus/metabolismo , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Proteínas de Plantas/metabolismo , Alineación de Secuencia , Factores de Transcripción/metabolismo
4.
Environ Res ; 188: 109660, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32502684

RESUMEN

In this study, the main influence factors of combined UV/O3 process in practical industrial application were explored through laboratory trials and industrial pilot tests. Dimethyl phthalate (DMP) was analyzed as the research subject through different experiments in laboratory. The degradation effect of organic compounds by O3 and UV/O3 processes in different air distribution methods was compared independently, and the mechanism of free radical generation by the two processes was analyzed. This study found that the combined UV/O3 process for organic matter mineralization is clearly better than that of independent effect of O3 process as mixed gas-liquid distribution method was superior to the bubble aeration method. The experimental conditions included inlet O3 concentration between 70 and 75 mg/L, reactor internal relative pressure at 0.3 MPa, contact reaction time of 12 min, DMP mineralization efficiency reaching 63.07%. The calculated dosing ratio of O3 in the dynamic experiment was around 0.74 mg CODCr/mg O3. The results showed that the best effect in wastewater treatment was achieved when the conditions of ultraviolet lamp irradiation intensity and the O3 dosage reached 822.88 W/m2 15 mg/L and utilized in conjunction with biochemical reactions. The resulting CODCr concentration of effluent reached 39.8 mg/L. Finally, it is determined that the main influence factors affecting the economically efficient operation of UV/O3 process were the efficient O3 distribution mode, control of the relative pressure within the reactor, proportion of ozone addition and light source configuration.


Asunto(s)
Ozono , Contaminantes Químicos del Agua , Purificación del Agua , Oxidación-Reducción , Rayos Ultravioleta , Eliminación de Residuos Líquidos , Aguas Residuales/análisis
5.
Hereditas ; 157(1): 14, 2020 Apr 20.
Artículo en Inglés | MEDLINE | ID: mdl-32312318

RESUMEN

BACKGROUND: Jerusalem artichoke (Helianthus tuberosus) is a fructan-accumulating plant, and an industrial source of raw material for fructan production, but the crucial enzymes involved in fructan biosynthesis remain poorly understood in this plant. RESULTS: In this study, a fructan: fructan 1-fructosyl-transferase (1-FFT) gene, Ht1-FFT, was isolated from Jerusalem artichoke. The coding sequence of Ht1-FFT was 2025 bp in length, encoding 641 amino acids. Ht1-FFT had the type domain of the 1-FFT protein family, to which it belonged, according to phylogenetic tree analysis, which implied that Ht1-FFT had the function of catalyzing the formation and extension of beta-(2,1)-linked fructans. Overexpression of Ht1-FFT in the leaves of transgenic tobacco increased fructan concentration. Moreover, the soluble sugar and proline concentrations increased, and the malondialdehyde (MDA) concentration was reduced in the transgenic lines. The changes in these parameters were associated with increased stress tolerance exhibited by the transgenic tobacco plants. A PEG-simulated drought stress experiment confirmed that the transgenic lines exhibited increased PEG-simulated drought stress tolerance. CONCLUSIONS: The 1-FFT gene from Helianthus tuberosus was a functional fructan: fructan 1-fructosyl-transferase and played a positive role in PEG-simulated drought stress tolerance. This transgene could be used to increase fructan concentration and PEG-simulated drought stress tolerance in plants by genetic transformation.


Asunto(s)
Sequías , Helianthus/enzimología , Hexosiltransferasas/genética , Nicotiana/fisiología , Estrés Fisiológico , Helianthus/genética , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/fisiología , Nicotiana/genética
6.
Hereditas ; 156: 9, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30774580

RESUMEN

BACKGROUND: Jerusalem artichoke (Helianthus tuberosus L.) is a characteristic crop in the Qinghai-Tibet Plateau which has rapidly developed and gained socioeconomic importance in recent years. Fructans are abundant in tubers and represent the foundation for their formation, processing and utilization of yield; and are also widely used in new sugar-based materials, bioenergy processing, ecological management, and functional feed. To identify key genes in the metabolic pathway of fructans in Jerusalem artichoke, high-throughput sequencing was performed using Illumina Hi Seq™ 2500 equipment to construct a transcriptome library. RESULTS: Qinghai-Tibet Plateau Jerusalem artichoke "Qingyu No.1" was used as the material; roots, stems, leaves, flowers and tubers of Jerusalem artichoke in its flowering stage were mixed into a mosaic of the Jerusalem artichoke transcriptome library, obtaining 63,089 unigenes with an average length of 713.6 bp. Gene annotation through the Nr, Swiss Prot, GO, KOG and KEGG databases revealed 34.95 and 46.91% of these unigenes had similar sequences in the Nr and Swiss Prot databases. The GO classification showed the Jerusalem artichoke unigenes were divided into three ontologies, with a total of 49 functional groups encompassing biological processes, cellular components, and molecular functions. Among them, there were more unigenes involved in the functional groups for cellular processes, metabolic processes, and single-organism processes. 38,999 unigenes were annotated by KOG and divided into 25 categories according to their functions; the most common annotation being general function prediction. A total of 13,878 unigenes (22%) were annotated in the KEGG database, with the largest proportion corresponding to pathways related to carbohydrate metabolism. A total of 12 unigenes were involved in the synthesis and degradation of fructan. Cluster analysis revealed the candidate 12 unigene proteins were dispersed in the 5 major families of proteins involved in fructan synthesis and degradation. The synergistic effect of INV gene is necessary during fructose synthesis and degradation in Jerusalem artichoke tuber development. The sequencing data from the transcriptome of this species can provide a reliable data basis for the identification and assessment of the expression of the members of the INV gene family.A simple sequence repeat (SSR) loci search was performed on the transcriptome data of Jerusalem artichoke, identifying 6635 eligible SSR loci with a large proportion of dinucleotide and trinucleotide repeats, and the most different motifs were repeated 5 times and 6 times. Dinucleotide and trinucleotide repeat motifs were the most frequent, with AG/CT and ACC/GGT repeat motifs accounting for the highest proportion. CONCLUSIONS: In this study, a database search of the transcriptome of the Jerusalem artichoke from the Qinghai Tibet Plateau was conducted by high throughput sequencing technology to obtain important transcriptional and SSR loci information. This allowed characterization of the overall expression features of the Jerusalem artichoke transcriptome, identifying the key genes involved in metabolism in this species. In turn, this offers a foundation for further research on the regulatory mechanisms of fructan metabolism in Jerusalem artichoke.


Asunto(s)
Fructanos/biosíntesis , Helianthus/genética , Transcriptoma , Metabolismo de los Hidratos de Carbono/genética , Helianthus/metabolismo , Secuenciación de Nucleótidos de Alto Rendimiento , Repeticiones de Microsatélite , Anotación de Secuencia Molecular , Tibet
7.
iScience ; 27(2): 108952, 2024 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-38357668

RESUMEN

In response to escalating environmental concerns surrounding antibiotic pollution, the utilization of calcium-montmorillonite minerals for tetracycline wastewater treatment is gaining prominence. This study systematically analyzed the physicochemical properties of calcium-montmorillonite through scanning electron microscope, contact angle analysis, X-ray diffraction, and Fourier transform infrared spectroscopy. It explored the adsorption efficacy and mechanisms for tetracycline removal, considering factors like initial pH, adsorption duration, concentration, and co-cations (Na+ and Ca2+). Under optimized conditions, achieving over 90% tetracycline removal with a maximum adsorption capacity of 526 mg/g, the study revealed competitive adsorption sites for coexisting cations. The Langmuir model best described the monolayer adsorption process, while kinetic studies favored the pseudo-first-order model. This research offers comprehensive insights into tetracycline adsorption on calcium-montmorillonite, emphasizing its potential as an efficient, cost-effective adsorbent for pharmaceutical wastewater treatment.

8.
ACS Omega ; 9(1): 573-584, 2024 Jan 09.
Artículo en Inglés | MEDLINE | ID: mdl-38222656

RESUMEN

This study utilized a 1% chitosan solution (dissolved in 2% acetic acid), with a chitosan-to-zeolite mass ratio of 0.005, to successfully prepare chitosan-loaded natural zeolite. The performance of chitosan-modified natural zeolite in the removal of low-concentration cadmium ions in the presence of micropollutants was investigated. The adsorbent was characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM)/energy-dispersive spectroscopy (EDS) techniques. The impact of modified adsorbent dosage, pH value, contact time, temperature, and initial concentration on adsorption performance was discussed. Additionally, the adsorption kinetics, isotherms, and thermodynamics of cadmium on chitosan-modified zeolites were analyzed. The results indicated that the modified zeolite exhibited a dispersed and porous structure with increased surface area, average pore size, and total pore volume. Under the conditions of 25 °C, pH 6, a dosage of 8 g/L, and a 60 min adsorption reaction time, chitosan-loaded natural zeolite (CNZ) achieved a removal efficiency of over 94.51% for a 100 µg/L cadmium solution (in a 100 mL volume). The adsorption process followed the Langmuir model, suggesting monolayer adsorption. The adsorption kinetics followed a pseudo-second-order equation, indicating an exothermic process with an increase in entropy. Chitosan-loaded natural zeolite demonstrated improved adsorption capacity and effectively removed cadmium from water contaminated with micropollutants.

9.
Sci Total Environ ; 919: 170757, 2024 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-38340851

RESUMEN

Water resources carrying capacity (WRCC) is indispensable for sustainable development, acting as a crucial determinant for harmonizing ecological preservation with socio-economic advancement. This research delineates an advanced evaluation index system for WRCC, focusing on Henan Province, China, a region straddling the Yangtze, Huaihe, Yellow, and Haihe river basins. Leveraging the analytic hierarchy process (AHP) with a system dynamics (SD) model, our analysis dissects the nonlinear interplays among demographic expansion, economic activities, land use patterns, water resources, and water environment. We introduce a novel integration of the "Four Determinations with Water" principle with sustainable development tenets, thereby sculpting six exploratory scenarios that chart Henan's potential paths from 2022 to 2035. Through these scenarios, we forecast and scrutinize the evolution of population dynamics, GDP, water supply, and sewage discharge volumes, employing rigorous quantitative analyses for a holistic evaluation. The results show that: WRCC in Henan Province becomes larger gradually, and, in Scenario 6, the WRCC indicator is the largest (0.643 in 2035) and the prediction effect is the best, while in Scenario 1, the WRCC indicator is the smallest (0.472 in 2035) and the prediction effect is the worst. Based on the prediction results, suggestions were made to adjust the industrial structure and strengthen the awareness of water conservation to improve the regional water resources carrying capacity.

10.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 35(6): 672-6, 2013 Dec.
Artículo en Zh | MEDLINE | ID: mdl-24382248

RESUMEN

OBJECTIVE: To systematically review the role of two common prostheses(metal on metal and metal on polythene)in total hip replacement. METHODS: Studies on two prostheses(metal on metal and metal on polythene)in total hip replacement were searched in databases including PubMed, EMBASE, the Cochrane library, CNKI database, WANFANG database, and VIP database using key words including"metal on metal", "metal on polythene", and "total hip replacement". Meta-analysis was performed using RevMan 5.2 software. RESULTS: Metal on metal group was superior to metal on polythene group in terms of Harris function evaluation(WMD=4.40, 95%CI: 3.50-5.31, P<0.01), operation time(WMD=6.82, 95%CI: 4.50-9.13, P<0.01), whereas other indicators showed no significant difference between these two groups. CONCLUSIONS: Compared with the prosthesis, metal on metal is better than metal on polythene in improving the Harris function when applied for total hip replacement. However, more high-quality large-scale randomized controlled trials are required to further verify it.


Asunto(s)
Artroplastia de Reemplazo de Cadera , Prótesis e Implantes , Humanos
11.
Front Public Health ; 11: 1298875, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-38249395

RESUMEN

Background: Promoting high-level coupling coordination between the provision of medical services (PMS) and high-quality economic development (HED) has emerged as a critical issue in China's pursuit of high-quality development and is now a significant subject of concern in the area of welfare economics. Materials and methods: Based on panel data from 11 provinces and municipalities in the Yangtze River Economic Belt, covering the period from 2010 to 2020, this study conducted an empirical analysis of the coupling coordination between PMS and HED and its influencing factors. Methods combined a comprehensive evaluation model, a coupling coordination model, and a panel Tobit model. Results: The study found that: (1) Regarding the overall situation in the Yangtze River Economic Belt, the overall PMS demonstrates a fluctuating upward trend, while HED fluctuates within the lower range of 0.3 to 0.4. The coupling coordination degree between PMS and HED fluctuates within the moderate range of 0.5 to 0.6. (2) In terms of the spatiotemporal evolution trends, there still exists substantial spatial disparity among provinces and municipalities within the Yangtze River Economic Belt regarding PMS; nonetheless, this gap is gradually narrowing. Significant regional disparities are also observed in HED, with Shanghai, Jiangsu, and Zhejiang leading among the provinces and municipalities in the Yangtze River Economic Belt. The coupling coordination degree between PMS and HED displays notable spatial discrepancies, where downstream areas of the Yangtze River Economic Belt such as Shanghai, Jiangsu, and Zhejiang exhibit a higher coupling coordination degree compared to other provinces and municipalities. However, most provinces and municipalities outside this group remain at a moderately coordinated stage concerning the degree of coupling coordination between PMS and HED. (3) Economic development level and local government competition had a significant negative impact on coupling coordination between PMS and HED, whereas there was a significantly positive impact on the degree of fiscal autonomy and urbanization. Discussion: This study contributes to comprehensively understanding the coupling and coordination relationship between the PMS and HED across provinces and municipalities in the Yangtze River Economic Belt. It provides empirical evidence for the collaborative evolution of PMS and HED.


Asunto(s)
Desarrollo Económico , Gobierno Local , China , Ríos
12.
Plants (Basel) ; 12(4)2023 Feb 08.
Artículo en Inglés | MEDLINE | ID: mdl-36840111

RESUMEN

The principal objective of this study was to screen and verify reference genes appropriate for gene expression evaluation during plant growth and development under distinct growth conditions. Nine candidate reference genes were screened based on garlic transcriptome sequence data. RT-qPCR was used to detect the expression levels of the aforementioned reference genes in specific tissues under drought and cold stress. Then, geNorm, NormFinder, BestKeeper, and ReFinder were used to consider the consistency of the expression levels of candidate reference genes. Finally, the stress-responsive gene expression of ascorbate peroxidase (APX) was quantitatively evaluated to confirm the chosen reference genes. Our results indicated that there were variations in the abundance and stability of nine reference gene transcripts underneath cold and drought stress, among which ACT and UBC-E2 had the highest transcript abundance, and 18S rRNA and HIS3 had the lowest transcript abundance. UBC and UBC-E2 were the most stably expressed genes throughout all samples; UBC and UBC-E2 were the most stably expressed genes during cold stress, and ACT and UBC were the most stably expressed genes under drought stress. The most stably expressed genes in roots, pseudostems, leaves, and cloves were EF1, ACT, HIS3, UBC, and UBC-E2, respectively, while GAPDH was the most unstable gene during drought and cold stress conditions and in exclusive tissues. Taking the steady reference genes UBC-E2, UBC, and ACT as references during drought and cold stress, the reliability of the expression levels was further demonstrated by detecting the expression of AsAPX. Our work thereby offers a theoretical reference for the evaluation of gene expression in garlic in various tissues and under stress conditions.

13.
Heliyon ; 9(8): e18974, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37636388

RESUMEN

Pepino (Solanum muricatum), a member of the Solanaceae family originating from South America, is cultivated globally. However, the cultivation range and suitable habitat of Pepino have not been extensively studied, which hampers the further development of its cultivation industry. Therefore, we aimed at enrich and expand the planting scope of Pepino. Currently, the main cultivation areas of Pepino in China are the Yunnan-Guizhou Plateau and the Loess Plateau, where the altitude is above 1000 m. In this study, ArcGIS combined with the MaxEnt model was used for prediction, whose area under curve value was 0.949. The main climatic factors affecting the distribution of Pepino are temperature seasonality, annual means temperature, mean temperature of the coldest quarter, elevation, isothermality, and the climate factors, and their cumulative contribution rate of 87.6%. Pepino's main potential distribution areas are located in Yunnan-Guizhou Plateau, Yunnan Province, Hexi Corridor of Loess Plateau, and low altitude areas of Qinghai-Tibet Plateau. The main distribution ranges from 1000 to 2000 m above sea level, and the total suitable area accounts for 20.09% of China's total land area. The prediction results reveal an expanded potential area for Pepino, with no significant migration in the central region of the main potential distribution area by 2050 and 2070. No studies have been conducted on the open-area cultivation of Pepino in northern China. Our findings revealed that the yield and quality in the four experimental sites and final actual cultivation conditions were consistent with the predicted results of MaxEnt. The yiel d per plant in Xunhua and Minhe was significantly different from that in Xining, which was low, and that in Minhe was the highest. Overall, the fruit quality in the Xining region was the lowest among the three regions, which was related to the climatic differences in each region. These results align with the predicted outcomes, indicating that Xining is the least suitable area. Further, these data verify the accuracy of the prediction results. The climate data of the four regions were analyzed simultaneously to elucidate the influence of different climate conditions on the growth of Pepino. Our findings are of considerable significance for introducing characteristic horticultural crops in the Qinghai-Tibet Plateau and using the MaxEnt model to predict the cultivation range of crops.

14.
Sci Total Environ ; 866: 161247, 2023 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-36603646

RESUMEN

Polystyrene nanoplastics (PSNPs, <100nm), an artificial pollutant that is widespread in the environment, can be assimilated by plants to alter plant gene expression and its metabolic pathway; thus, interfering with physiological homeostasis and growth of plants. Recently, the biosafety and potential environmental risks of PSNPs have attracted enormous attention. However, the knowledge regarding the uptake and phytotoxicity of atmosphere PSNPs subsiding to plant leaves is still limited. Here, we separately applied 50 mg/L and 100 mg/L PSNPs on cucumber leaves to simulate the plant response to the atmosphere PSNPs. We found that the PSNPs can be accumulated on the surface of cucumber leaves and are also able to be uptake by cucumber leaf stomata. The repertoires of metabolomics and transcriptomics from cucumber leaves upon PSNPs treatment demonstrated that the deposition of PSNPs on leaves alters the biosynthesis of various metabolites and the expression of a variety of genes. The leaves exposure to low concentration (50 mg/L) of PSNPs impact the genes involved in carbohydrate metabolism and the biosynthesis of metabolites related to membrane stability maintenance, thereby, probably enhancing plant tolerance to the stress caused by PSNPs. Whereas, exposure to high concentration (100 mg/L) of PSNPs, both nitrogen and carbohydrate metabolism in cucumber leaves are affected, as well as that the photosynthetic capacity was decreased, leading to the threat to plant health. Combined omics technologies, our findings advance our understanding about how the PSNPs released to ecological environment influence the terrestrial plant growth and provide phytotoxic mechanism.


Asunto(s)
Cucumis sativus , Poliestirenos/toxicidad , Poliestirenos/metabolismo , Microplásticos/metabolismo , Transcriptoma , Metabolómica , Hojas de la Planta/metabolismo
15.
Food Res Int ; 163: 112287, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36596193

RESUMEN

There is considerable knowledge about plant compounds that produce flavor, scent, and aroma. Aside from the similarities, however, groups of plant-produced nutrients and taste components have little in common with each other. Network analysis holds promise for metabolic gene discovery, which is especially important in plant systems where metabolic networks are not yet fully resolved. To bridge this gap, we propose a joint model of gene regulation and metabolic reactions in two different pepino varieties. Differential metabolomics analysis is carried out for detection of eventual interaction of compound. We adopted a multi-omics approach to profile the transcriptome and metabolome analyze differences in phenolic acids, flavonoids, organic acids, lipids, alkaloids, and sugars between LOF and SRF. The two most predominant classes of metabolites are phenolic acids and lipids in pepino. Overall results show enrichment in most DEGs was carbohydrate and biosynthesis of secondary metabolites pathway. Results of DEMs predominantly comprised N-p-coumaroyl agmatine and tryptamine, and significant differences were observed in their expression between LOF and SRF. Integrated DEMs and DEGs specific networks were constructed by combining two types of networks: transcriptional regulatory networks composed of interactions between DEMs and the regulated genes, and pepino metabolite-metabolite interaction networks. Newly discovered features, such as DEGs (USPA, UBE2 and DELLA) involved in the production of secondary metabolites are found in coregulated gene clusters. Moreover, lipid metabolites were most involved in DEMs correlations by OPLS-DA while identifying a significant number of DEGs co-regulated by SENP1, HMGCS et al. These results further that the metabolite discrepancies result from characterized the nutrients and taste components between two pepino genotype. Among the possible causes of the differences between species in pepino metabolite concentrations is co-regulated by these DEGs, continue to suggest that novel features of metabolite biosynthetic pathway remain to be uncovered. Finally, the integrated metabolome and transcriptome analyses have revealed that many important metabolic pathways are regulated at the transcriptional level. The metabolites content differences observed among varieties of the same species mainly originates from different regulated genes and enzymes expression. Overall, this study provides new insights into the underlying causes of differences in the plant metabolites and suggests that genetic data can be used to improve its nutrients and taste components.


Asunto(s)
Solanum , Gusto , Transcriptoma , Nutrientes , Lípidos
16.
Cells ; 12(7)2023 03 26.
Artículo en Inglés | MEDLINE | ID: mdl-37048089

RESUMEN

Plant-specific transcription factors such as the TCP family play crucial roles in light responses and lateral branching. The commercial development of S. muricatum has been influenced by the ease with which its lateral branches can be germinated, especially under greenhouse cultivation during the winter with supplemented LED light. The present study examined the TCP family genes in S. muricatum using bioinformatics analysis (whole-genome sequencing and RNA-seq) to explore the response of this family to different light treatments. Forty-one TCP genes were identified through a genome-wide search; phylogenetic analysis revealed that the CYC/TB1, CIN and Class I subclusters contained 16 SmTCP, 11 SmTCP and 14 SmTCP proteins, respectively. Structural and conserved sequence analysis of SmTCPs indicated that the motifs in the same subcluster were highly similar in structure and the gene structure of SmTCPs was simpler than that in Arabidopsis thaliana; 40 of the 41 SmTCPs were localized to 12 chromosomes. In S. muricatum, 17 tandem repeat sequences and 17 pairs of SmTCP genes were found. We identified eight TCPs that were significantly differentially expressed (DETCPs) under blue light (B) and red light (R), using RNA-seq. The regulatory network of eight DETCPs was preliminarily constructed. All three subclusters responded to red and blue light treatment. To explore the implications of regulatory TCPs in different light treatments for each species, the TCP regulatory gene networks and GO annotations for A. thaliana and S. muricatum were compared. The regulatory mechanisms suggest that the signaling pathways downstream of the TCPs may be partially conserved between the two species. In addition to the response to light, functional regulation was mostly enriched with auxin response, hypocotyl elongation, and lateral branch genesis. In summary, our findings provide a basis for further analysis of the TCP gene family in other crops and broaden the functional insights into TCP genes regarding light responses.


Asunto(s)
Arabidopsis , Solanum , Solanum/genética , Solanum/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Secuencia de Aminoácidos , Arabidopsis/genética , Perfilación de la Expresión Génica
17.
Int J Oncol ; 63(4)2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37594082

RESUMEN

Glioblastoma multiforme (GBM) is the most frequent and lethal cancer derived from the central nervous system, of which the mesenchymal (MES) subtype seriously influences the survival and prognosis of patients. NAD(P)H: quinone acceptor oxidoreductase 1 (NQO1) serves an important role in the carcinogenesis and progression of various types of cancer; however, the specific mechanism underlying the regulatory effects of NQO1 on GBM is unclear. Thus, the present study aimed to explore the role and mechanism of NQO1 in GBM progression. The results of bioinformatics analysis and immunohistochemistry showed that high expression of NQO1 was significantly related to the MES phenotype of GBM and shorter survival. In addition, MTT, colony formation, immunofluorescence and western blot analyses, and lung metastasis model experiments suggested that silencing NQO1 inhibited the proliferation and metastasis of GBM cells in vitro and in vivo. Furthermore, western blotting showed that the activity of the PI3K/Akt/mTOR signaling pathway was revealed to be inhibited by downregulation of NQO1 expression, whereas it was enhanced by overexpression of NQO1. Notably, co­immunoprecipitation and ubiquitination experiments suggested that Snail was considered an important downstream target of NQO1 in GBM cells. Snail knockdown could eliminate the promoting effect of ectopic NQO1 on the proliferation and invasion of GBM cells, and reduce its effects on the activity of PI3K/Akt/mTOR signaling pathway. These results indicated that NQO1 could promote GBM aggressiveness by activating the PI3K/Akt/mTOR signaling pathway in a Snail­dependent manner, and NQO1 and its relevant pathways may be considered novel targets for GBM therapy.


Asunto(s)
Glioblastoma , Humanos , Glioblastoma/genética , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Serina-Treonina Quinasas TOR/genética , Agresión , NAD , NAD(P)H Deshidrogenasa (Quinona)/genética
18.
Plant Signal Behav ; 18(1): 2283363, 2023 Dec 31.
Artículo en Inglés | MEDLINE | ID: mdl-37976083

RESUMEN

Pepino (Solanum muricatum), a horticultural crop that has experienced significant growth in the highlands of China over the past two decades, is widely embraced by consumers due to its distinctive taste and nutritional advantages. This study focused on the cultivar 'Qingcanxiang' of pepino grown on the Qinghai-Tibetan Plateau was analyzed using UPLC-QTOF-MS and RNA-seq transcriptome sequencing. Fruit samples were collected at three distinct stages of development, and the results of the metabolomics and transcriptomics were compared and correlated. The study's findings indicate that the 'Qingcanxiang' fruit contained a total of 187 metabolites, comprising 12 distinct categories of compounds, including amino acids and their derivatives, organic acids, sugars and alcohols, phenols and phenolic acids. Of these metabolites, 94 were identified as differential. Significant variations in nutrient composition were observed across the three growth stages of the fruit. Specifically, the stage spanning from the growth to the maturation was identified as the critical stages for nutrient accumulation and flavor development. Transcriptome sequencing analysis revealed a set of highly associated genes between aspartate and quinic acid, namely SIR2, IRAK4, RP-L29, and CCNH. These genes are potentially involved in the regulation of both amino acid and phenolic acid synthesis. Through the application of metabolomics and transcriptomics, this investigation elucidates the alterations in metabolites and the underlying molecular regulatory mechanisms of pepino fruits during three growth stages. The findings furnish a theoretical foundation for the evaluation of nutritional quality and the enhancement of breeding strategies for pepino.


Asunto(s)
Solanum , Solanum/genética , Frutas , Fenoles , Metabolómica , China
19.
iScience ; 26(10): 107869, 2023 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-37736047

RESUMEN

Recent studies have demonstrated that CPT1A plays a critical role in tumor metabolism and progression. However, the molecular mechanisms by which CPT1A affects tumorigenicity during PAAD progression remain unclear. In the current research, the bioinformatics analysis and immunohistochemical staining results showed that CPT1A was overexpressed in PAAD tissues and that its overexpression was associated with a shorter survival time in patients with PAAD. Overexpression of CPT1A increased cell proliferation and promoted EMT and glycolytic metabolism in PAAD cells. Mechanistically, CPT1A is able to bind to Snail and facilitate PAAD progression by regulating Snail stability. In summary, our findings revealed Snail-dependent glycolysis as a crucial metabolic pathway by which CPT1A accelerates PAAD progression. Targeting the CPT1A/Snail/glycolysis axis in PAAD to suppress cell proliferation and metastatic dissemination is a new potential treatment strategy to improve the anticancer therapeutic effect and prolong patient survival.

20.
Adv Healthc Mater ; 12(30): e2301645, 2023 12.
Artículo en Inglés | MEDLINE | ID: mdl-37557883

RESUMEN

Developing efficient integrated diagnosis and treatment agents based on fuel-free self-movement nanomotors remains challenging in antitumor therapy. In this study, a covalent organic framework (COF)-based biomimetic nanomotor composed of polypyrrole (PPy) core, porphyrin-COF shell, and HCT116 cancer cell membrane coating is reported. Under near-infrared (NIR) light irradiation, the obtained mPPy@COF-Por can overcome Brownian motion and achieves directional motion through self-thermophoretic force generated from the PPy core. The HCT116 cancer cell membrane coating enables the nanomotor to selectively recognize the source cell lines and reduces the bio-adhesion of mPPy@COF-Por in a biological medium, endowing with this NIR light-powered nanomotor good mobility. More importantly, such multifunctional integration allows the COF-based nanomotor to be a powerful nanoagent for cancer treatment, and the high infrared thermal imaging/photoacoustic imaging/fluorescence trimodal imaging-guided combined photothermal/photodynamic therapeutic effect on HCT116 tumor cell is successfully achieved. The results offer considerable promise for the development of COF nanomotors with integrated imaging/therapy modalities in biomedical applications.


Asunto(s)
Estructuras Metalorgánicas , Neoplasias , Humanos , Polímeros , Pirroles/farmacología , Medicina de Precisión , Neoplasias/terapia , Línea Celular Tumoral
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