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1.
J Biol Regul Homeost Agents ; 33(5): 1437-1449, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31637902

RESUMEN

Influenza has frequently been epidemic in recent years. However, the mechanisms of severe pneumonia with postinfluenza Streptococcus pneumoniae (SP) secondary infection have not been fully understood. In this study, we explored the mechanisms of pneumonia in postinfluenza A virus (IAV) infection via a mouse model. Mice were intranasally inoculated with SP three days after IAV inoculation. We then collected samples at three time points to dynamically observe the pathological progression. In IAV infection alone, lymphocyte infiltration and widened alveolar intervals were observed. In the blood, levels of the CD19+, CD19+CD21+ and CD19+CD79ß+B lymphocyte subpopulations were reduced, and IFN-γ and IL-10 were elevated. Slight atrophy was seen in the spleen, which was due to splenic B lymphocyteinitiated apoptosis through the mitochondrial pathway. When SP infection occurred after IAV infection, the pulmonary inflammation was significantly aggravated; a fair number of lymphocytes and neutrophils infiltrated simultaneously with exfoliated bronchial epithelial cells, vascular endothelial cells, widened alveolar septum and hemorrhaging. Increasing edema fluid and bacteria accumulated in the alveolar cavity. Decreased CD19+, CD19+CD21+ and CD19+CD79ß+B lymphocyte subpopulations and increased interferon gamma (IFN-γ) or interleukin 10 (IL-10) were more prominent compared to those with viral infection alone. Spleen atrophy resulting from coinfection was more obvious because of massive splenic B lymphocyte apoptosis through the mitochondrial pathway compared to viral infection alone. This study shows that although inflammation caused by SP infection alone was temporary, preceding IAV infection provided favorable conditions for SP colonization and multiplication by destroying lung structure and suppressing humoral immunity. Synergistic IAV-SP coinfection is likely to facilitate more SP colonization and promote B lymphocyte-suppression and reduction. Eventually, the pneumonia worsened.


Asunto(s)
Linfocitos B/inmunología , Infecciones por Orthomyxoviridae/inmunología , Infecciones Neumocócicas/inmunología , Neumonía Bacteriana/inmunología , Animales , Apoptosis , Linfocitos B/citología , Coinfección/microbiología , Coinfección/virología , Células Endoteliales , Virus de la Influenza A , Pulmón , Ratones , Infecciones por Orthomyxoviridae/microbiología , Infecciones Neumocócicas/virología , Streptococcus pneumoniae
2.
Vaccine ; 17(22): 2901-7, 1999 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-10438062

RESUMEN

To vaccinate against a vaginal challenge with Chlamydia trachomatis, C3H/HeJ (H-2k) mice were immunized intranasally (i.n.) or intraperitoneally (i.p.) with 1 x 10(6) inclusion forming units (IFU) of C. trachomatis, serovar E and i.n. with 1 x 10(6) UV inactivated IFU of serovar E. Animals inoculated i.n. with mock infected HeLa 229 cells were used as controls. Upon a vaginal challenge with 5 x 10(3) IFU of serovar E, mice immunized i.n. with viable serovar E exhibited significant protection as judged by the number of mice infected compared to controls (p < 0.05). In contrast, mice immunized i.n. with serovar E that had been UV-inactivated, were not protected from a subsequent vaginal challenge with serovar E. Mice immunized i.p. with serovar E showed attenuation of the infection by 4 weeks after challenge compared to control mice as to the number of animals with positive vaginal cultures (p < 0.05). Of the immune parameters examined, the best correlation with protection was seen with Chlamydia specific IgG and IgA vaginal titers and lymphoproliferative responses to serovar E. In summary, mucosal immunization with viable serovar E partially protected mice against a subsequent vaginal challenge, thereby showing that it is possible to elicit a protective response to a human strain of C. trachomatis at a distant mucosal site in this animal model.


Asunto(s)
Infecciones por Chlamydia/inmunología , Infecciones por Chlamydia/prevención & control , Chlamydia trachomatis/clasificación , Chlamydia trachomatis/inmunología , Vaginosis Bacteriana/prevención & control , Administración Intranasal , Animales , Técnicas de Tipificación Bacteriana , Vacunas Bacterianas/inmunología , Femenino , Células HeLa , Humanos , Inyecciones Intraperitoneales , Activación de Linfocitos/inmunología , Ratones , Ratones Endogámicos C3H , Vaginosis Bacteriana/inmunología , Vaginosis Bacteriana/microbiología
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