Combination of Loop-Mediated Isothermal Amplification Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples.

A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the fla gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.l. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.l. in human serum samples.

A Metaanalysis of physical activity among Chinese children and adolescents with accelerometer data / 中国学校卫生

Objective@#To provide baseline data for the development of physical activity and health promotion strategies and public health policy among Chinese children and adolescents by systematically evaluating accelerometermeasured moderatetovigorous physical activity (MVPA) and sedentary behavior(SB).@*Methods@#The study search was carried out using China National Knowledge Infrastructure, SPORTDiscus with Full Text, and Web of Science, including studies that published from January 1, 2009 to February 28, 2019. The studies were selected by inclusion (based on PICOS principles) and exclusion criteria, and were assessed through quality assessment items based on revised STROBE statement. The information about MVPA and SB were extracted, and Comprehensive Metaanalysis Software (CMA V2) was used to pool the findings from the included studies into a Metaanalysis.@*Results@#Sixteen studies published from 2012 to 2019 were included the Metaanalysis. Sample sizes of eligible studies ranged from 96 to 2 163 participants. Metaanalysis found that Chinese children and adolescents separately spent 37.66 min/day, 539.61 min/d in MVPA and SB. Boys were more likely to have higher level of MVPA in minutes compared to the girls’(P<0.01). Children and adolescents spent more time in MVPA on weekdays than weekends(P<0.05), and also spent more SB on weekdays than weekends(P<0.05).@*Conclusion@#Chinese children and adolescents are physically inactive and live a sedentary lifestyle in general, especially among girls. MVPA and SB time among children and adolescents spent on weekdays are higher than weekends.

Relationship between fundamental movement skills and physical fitness in children / 中国学校卫生

Objective@#To investigate the relationship between fundamental movement skills(FMS) and physical-related fitness(PF) in 5-6 aged children in Beijing and to provide the corresponding theoretical basis for the further development of the scien tific research work of children sports activities.@*Methods@#M-ABC-2(Movement Assessment Battery for Children-2)was used to assess FMS of the children aged 5-6 in 3 kindergartens of Beijing; “National Physical Fitness test Standards Manual-Preschool Part” was used as the standard for assessment of children’s PF; Canonical Correlation Analysis was used to analyze the relationship between FMS and PF.@*Results@#A total of 107 children’s complete data were obtained. The overall development level of 5-6 yearold children’s physical fitness could positively predict their the manual dexterity (B=0.24, P<0.05); In the test of individual PF, the muscle strength and balance ability exhibited by the tennis throwing and walking balance wood were also strongly correlated with the manual dexterity (r=0.43, P<0.01), and the standing long jump and the continuous jump of both feet also showed a positive correlation with the manual dexterity (r=0.35, P<0.05).@*Conclusion@#There is a certain relationship between the PF and FMS of children aged 5-6. The development of coordination and stability will play a positive role in promoting the development of their FMS. At the same time, parents and teachers should pay attention to the development of children's fine motor skills to promote their overall healthy growth.