Global profiling of protein lactylation in Caenorhabditis elegans.

Lactylation, as a novel posttranslational modification, is essential for studying the functions and regulation of proteins in physiological and pathological processes, as well as for gaining in-depth knowledge on the occurrence and development of many diseases, including tumors. However, few studies have examined the protein lactylation of one whole organism. Thus, we studied the lactylation of global proteins in Caenorhabditis elegans to obtain an in vivo lactylome. Using an MS-based platform, we identified 1836 Class I (localization probabilities > 0.75) lactylated sites in 487 proteins. Bioinformatics analysis showed that lactylated proteins were mainly located in the cytoplasm and involved in the tricarboxylic acid cycle (TCA cycle) and other metabolic pathways. Then, we evaluated the conservation of lactylation in different organisms. In total, 41 C. elegans proteins were lactylated and homologous to lactylated proteins in humans and rats. Moreover, lactylation on H4K80 was conserved in three species. An additional 238 lactylated proteins were identified in C. elegans for the first time. This study establishes the first lactylome database in C. elegans and provides a basis for studying the role of lactylation.

Design of Fluxgate Current Sensor Based on Magnetization Residence Times and Neural Networks.

This study introduces a novel fluxgate current sensor with a compact, ring-shaped configuration that exhibits improved performance through the integration of magnetization residence times and neural networks. The sensor distinguishes itself with a unique magnetization profile, denoted as M waves, which emerge from the interaction between the target signal and ambient magnetic interference, effectively enhancing interference suppression. These M waves highlight the non-linear coupling between the magnetic field and magnetization residence times. Detection of these residence times is accomplished using full-wave rectification circuits and a Schmitt trigger, with a digital output provided by timing sequence detection. A dual-layer feedforward neural network deciphers the target signal, exploiting this non-linear relationship. The sensor achieves a linearity error of 0.054% within a measurement range of 15 A. When juxtaposed with conventional sensors utilizing the residence-time difference strategy, our sensor reduces linearity error by more than 40-fold and extends the effective measurement range by 150%. Furthermore, it demonstrates a significant decrease in ambient magnetic interference.

Global analysis of lysine acetylation in the brain cortex of K18-hACE2 mice infected with SARS-CoV-2.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected hundreds of millions of people all over the world and thus threatens human life. Clinical evidence shows that SARS-CoV-2 infection can cause several neurological consequences, but the existing antiviral drugs and vaccines have failed to stop its spread. Therefore, an understanding of the response to SARS-CoV-2 infection of hosts is vital to find a resultful therapy. Here, we employed a K18-hACE2 mouse infection model and LC-MS/MS to systematically evaluate the acetylomes of brain cortexes in the presence and absence of SARS-CoV-2 infection. Using a label-free strategy, 3829 lysine acetylation (Kac) sites in 1735 histone and nonhistone proteins were identified. Bioinformatics analyses indicated that SARS-CoV-2 infection might lead to neurological consequences via acetylation or deacetylation of important proteins. According to a previous study, we found 26 SARS-CoV-2 proteins interacted with 61 differentially expressed acetylated proteins with high confidence and identified one acetylated SARS-CoV-2 protein nucleocapsid phosphoprotein. We greatly expanded the known set of acetylated proteins and provide the first report of the brain cortex acetylome in this model and thus a theoretical basis for future research on the pathological mechanisms and therapies of neurological consequences after SARS-CoV-2 infection.

Dynamic regulatory processes among child welfare parents: Temporal associations between physiology and parenting behavior.

This study examined how temporal associations between parents' physiological and behavioral responses may reflect underlying regulatory difficulties in at-risk parenting. Time-series data of cardiac indices (second-by-second estimates of inter-beat intervals - IBI, and respiratory sinus arrhythmia - RSA) and parenting behaviors were obtained from 204 child welfare-involved parents (88% mothers, Mage = 32.32 years) during child-led play with their 3- to 7-year-old children (45.1% female; Mage = 4.76 years). Known risk factors for maltreatment, including parents' negative social cognitions, mental health symptoms, and inhibitory control problems, were examined as moderators of intra-individual physiology-behavior associations. Results of ordinary differential equations suggested increases in parents' cardiac arousal at moments when they showed positive parenting behaviors. In turn, higher arousal was associated with momentary decreases in both positive and negative parenting behaviors. Individual differences in these dynamic processes were identified in association with parental risk factors. In contrast, no sample-wide RSA-behavior associations were evident, but a pattern of increased positive parenting at moments of parasympathetic withdrawal emerged among parents showing more total positive parenting behaviors. This study illustrated an innovative and ecologically-valid approach to examining regulatory patterns that may shape parenting in real-time and identified mechanisms that should be addressed in interventions.

Effect of Femoral Nerve Block with Different Concentrations of Chloroprocaine on Early Postoperative Rehabilitation Training After Total Knee Arthroplasty.

BACKGROUND Patients experience severe pain in early postoperative rehabilitation after total knee arthroplasty (TKA). This study aimed to compare the effect of femoral nerve block with different concentrations of chloroprocaine on postoperative rehabilitation in patients with TKA. MATERIAL AND METHODS Ninety patients who only received unilateral TKA were randomly and equally divided into C1 (1% chloroprocaine 0.2 ml/kg), C2 (2% chloroprocaine 0.2 ml/kg), or NS (0.9% sodium chloride solution 0.2 ml/kg) groups. The patients received rehabilitation 3 times a day on days 3-6 after surgery, and femoral nerve block was performed with corresponding solution 10 min before each training session. We recorded the maximum knee flexion angles (MKFA) and maximum knee extension angles (MKEA) during active exercise on day 7 after surgery, as well as the incidence of MKFA ³100°, American knee society (AKS) scores, and postoperative rehabilitation satisfaction. Adverse effects after administration in each group were also recorded. RESULTS Compared with group NS, patients in group C1 and C2 had larger MKFA during active exercise on day 7 after TKA, and had better rehabilitation satisfaction (P<0.05). MKEA, the incidence of MKFA ≥100°, and AKS scores showed no significant differences in the 3 groups. There were more patients with decline of muscle strength in group C2 (P<0.05), and no other adverse reactions were recorded. CONCLUSIONS Chloroprocaine for femoral nerve block can be safely used in rehabilitation after TKA and to improve the knee flexion angle in the early postoperative period. Because they may have fewer adverse effects, 1% chloroprocaine 0.2 ml/kg may be preferred.

A dynamic systems account of parental self-regulation processes in the context of challenging child behavior.

To advance the understanding of how parental self-regulation contributes to their role in supporting children's development, this study proposes a model of the dynamic processes involved in parental self-regulation. Based on time-series data from 157 mothers and their 30- to 60-month-old children (49.7% female; 96% White; data collected June 2017-December 2019 in central Pennsylvania, U.S.) during a challenging wait task, the model was tested by examining the temporal relations among challenging child behavior, maternal physiology, and maternal responsiveness. Results were consistent with the hypothesized dynamic negative feedback processes and revealed their associations with the overall quality of parenting behaviors and experiences. Findings elucidate how parents adapt to competing external (attending to child) and internal (restoring parents' equilibrium) demands during parenting challenges.

Clinical efficacy of melatonin as adjunctive therapy to non-surgical treatment of periodontitis: a systematic review and meta-analysis.

OBJECTIVE: This study aimed to evaluate the effect of adjunctive melatonin supplementation on clinical outcomes after non-surgical periodontal treatment. METHODS: PubMed, Embase, and Web of Science databases were systematically searched for randomised controlled trials (RCTs) of melatonin adjuvant therapy for periodontitis from inception until May 2021. The systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and registered on The International Prospective Register of Systematic Reviews (PROSPERO) (CRD42021250630). The risk of bias of included studies was assessed according to the Cochrane Handbook for Systematic Reviews of Interventions. The pooled effect estimates were calculated by a random-effects model, and results were expressed as weighted mean differences (WMD). RESULTS: Seven RCTs comprising 412 participants were included in the meta-analysis. The pooled results showed that adjuvant use of melatonin for non-surgical periodontal treatment significantly improved the probing depth (PD) [WMD = - 1.18, 95% CI (- 1.75, - 0.62) I2 = 85.7%], clinical attachment loss (CAL) [WMD = - 1.16, 95% CI (- 1.60, - 0.72) I2 = 76.7%] and gingival index (WMD = - 0.29, 95%CI [- 0.48, - 0.11], I2 = 63.6%) compared with non-surgical treatment alone. In addition, subgroup analysis showed that higher doses of melatonin (3-10 mg) significantly improved PD [WMD = - 1.32, 95%CI (- 2.31, - 0.15) I2 = 93%] and CAL [WMD = - 1.30, 95%CI (- 1.80, - 0.81) I2 = 73.7%] compared with lower doses of melatonin (< 3 mg). CONCLUSIONS: We found that adjunctive melatonin supplementation can significantly improve the periodontal status after non-surgical treatment, suggesting that melatonin may be a new adjuvant therapy for periodontitis when non-surgical periodontal treatment alone cannot achieve the desired improvement.

Early executive and school functioning: Protective roles of home environment by income.

This study used data from the Family Life Project (N=1,227), a longitudinal study of child development. We tested a three-way interaction in which positive parenting and learning materials in the home from age 6-36 months and family income predicted children's executive functioning (EF) at 58 months. We also tested whether this interaction predicted early school functioning, specifically behavioral and academic skills in the 1st grade. The interactive effects of positive parenting and learning materials differed by family income. For children in families of lower income, more learning materials and positive parenting predicted better EF, and in turn, better early school functioning. For children in families of higher income, only positive parenting significantly predicted EF, which in turn, predicted better early school functioning. Findings suggest that more targeted policy and program support for enrichment and positive parenting may bolster efforts to combat poverty.

Perceived Child Difficultness, Emotion Dysregulation, and Emotion-Related Parenting among Chinese Parents.

This study investigated the integrative effects of parents' perceptions of child difficultness and parental emotion dysregulation on emotion-related parenting among a group of Chinese parents of school-age children. One hundred and fifty parent-child dyads (121 biological mothers and 29 biological fathers as primary caregivers; Mage = 39.22 years) from urban Beijing, China participated in the study. Parents reported on their own emotion dysregulation and their children's difficultness, as well as their emotion socialization practices. Children (Mage = 8.54 years; ranged from 6 to 12 years) reported on their parents' use of psychological control strategies. Main and interactive effects were tested using path analysis. Results indicated that parents' perceived child difficultness was negatively associated with supportive reactions to children's expression of negative emotions, and parental emotion dysregulation was positively associated with unsupportive reactions. When parents perceived their children to be difficult and also reported emotion regulation difficulties of their own, they showed the highest levels of psychological control (child reports). These findings suggest differential effects of parent cognition and emotion on supportive and unsupportive reactions to children's negative emotions. Both cognition and emotion play important roles in relation to parents' use of psychological control.

En este estudio se investigaron los efectos integradores de la dificultad de los niños percibida por los padres y la desregulación emocional parental en la crianza relacionada con las emociones entre un grupo de padres chinos de niños en edad escolar. En el estudio participaron ciento cincuenta díadas de padres e hijos (121 madres biológicas y 29 padres biológicos como cuidadores principales; edad promedio = 39.22 años) del área urbana de Beijing, China. Los padres informaron su propia desregulación emocional y la dificultad de sus hijos, así como sus prácticas de socialización emocional. Los niños (edad promedio = 8.54 años; de entre 6 y 12 años) informaron sobre el uso de estrategias de control psicológico de sus padres. Los efectos principales e interactivos se evaluaron usando el análisis de ruta. Los resultados indicaron que la dificultad de los niños percibida por los padres estuvo asociada negativamente con reacciones de apoyo a la expresión de emociones negativas de los niños, y la desregulación emocional parental estuvo asociada positivamente con reacciones de falta de apoyo. Cuando los padres percibieron que sus hijos eran difíciles y también informaron dificultades propias de regulación emocional, demostraron niveles más altos de control psicológico (informes de los niños). Estos resultados sugieren efectos diferenciales de cognición y emoción de los padres en las reacciones de apoyo y de falta de apoyo a las emociones negativas de los niños. Tanto la cognición como la emoción cumplen funciones importantes en relación con el uso del control psicológico de los padres.

The developmental cascade of early parenting, emergence of executive functioning, and emotional symptoms across childhood.

Many studies focus on proximal associations between parental sensitivity and emotional well-being in early childhood, with less data examining how parenting in infancy predicts children's emotional trajectories across childhood, in particular negative emotional symptoms of anxiety and depression. Thus, this study utilized data from The Family Life Project (N = 1015), a prospective study of child development in rural poverty, and assessed whether sensitive parenting in the first 4 years of life predicted child internalizing emotional symptoms (i.e., anxiety and depression) from kindergarten to fifth grade and whether early child executive functioning mediated this relationship. Path models indicated that observation of sensitive parenting predicted a decrease in teachers' report of child negative emotional symptoms over time and predicted fewer negative emotional symptoms in fifth grade. Moreover, though executive functioning performance did not mediate change in symptoms over time, executive functioning did mediate the relationship between sensitive parenting and fifth-grade symptoms, suggesting that executive functioning is one mechanism by which early sensitive parenting buffers long-term emotional development. Findings highlight the importance of early parenting and executive functioning in development of anxiety and depression symptoms and suggests potential targets for transdiagnostic intervention.

Muchos estudios se enfocan en asociaciones proximales entre la sensibilidad del progenitor y el bienestar emocional en la temprana niñez, aunque una menor información examina cómo la crianza en la infancia predice las trayectorias emocionales de los niños a lo largo de la niñez, particularmente los síntomas emocionales de ansiedad y depresión. Por tanto, este estudio utilizó datos de [BLINDED FOR REVIEW] (N = 1,015), un estudio en prospecto del desarrollo del niño en la pobreza rural, y evaluó si la crianza sensible en los primeros 4 años de vida predijo los síntomas emocionales de internalización en el niño (v.g. ansiedad y depresión) a partir del kínder hasta el quinto grado y si el temprano funcionamiento ejecutivo del niño mediaba esta relación. Los modelos de trayecto indicaron que la observación de la crianza sensible predijo una baja en los reportes de los maestros sobre síntomas emocionales negativos en el niño a lo largo del tiempo y predijo menos síntomas emocionales negativos en el quinto grado. Es más, a pesar de que el rendimiento del funcionamiento ejecutivo no medió el cambio en los síntomas a lo largo del tiempo, el funcionamiento ejecutivo sí medió la relación entre la crianza sensible y los síntomas en el quinto grado, lo cual sugiere que el funcionamiento ejecutivo es un mecanismo por medio del cual la temprana crianza sensible amortigua el desarrollo emocional a largo plazo. Los resultados subrayan la importancia de la crianza temprana y el funcionamiento ejecutivo en el desarrollo de los síntomas de ansiedad y depresión y sugiere metas probables para la intervención de transdiagnosis.

Beaucoup d'études portent sur les liens proximaux entre la sensibilité parentale et le bien-être émotionnel dans la petite enfance, avec moins de données examinant comment le parentage dans la petite enfance prédit les trajectoires émotionnelles des enfants durant l'enfance, en particulier les symptômes émotionnels négatifs d'anxiété et de dépression. Donc, cette étude a utilisé des données du Project de Vie Familiale (en anglais Family Life Project) (N = 1015), une étude prospective du développement de l'enfant en pauvreté rurale, et évalué si la sensibilité de parentage dans les quatre premières années de vie prédisaient l'internalisation de symptômes émotionnels de la part de l'enfant (comme l'anxiété et la dépression) de la maternelle au CM2 (ou 5ème au Québec) et si le fonctionnement exécutif précoce de l'enfant servait de médiateur à la relation. Les analyses causales ont indiqué que l'observation d'un parentage sensible prédisait une baisse des rapports faits par les enseignants de symptômes émotionnels négatifs de la part des enfants et prédisaient moins de symptômes émotionnels négatifs au CM2 (5ème au Québec). De plus, bien que la performance du fonctionnement exécutif n'a pas médié les changements dans les symptômes au fil du temps, le fonctionnement exécutif a médié la relation entre le parentage sensible et les symptômes au CM2 (5ème au Québec), suggérant que le fonctionnement exécutif est un mécanisme par lequel le parentage sensible précoce sert de tampon au développement émotionnel à long terme. Les résultats mettent en lumière l'importance du parentage précoce et du fonctionnement exécutif dans le développement de symptômes d'anxiété et de dépression et suggèrent des cibles potentielles pour une intervention trans-diagnostique.

When you go low, I go high: Negative coordination of physiological synchrony among parents and children.

The parent-child relationship is an important context for children's emotional development. The current study assessed 43 parent-child dyads while watching a positive film clip and a negative film clip, in order to examine whether partner's physiological reactivity demonstrated dyadic synchrony, and whether this was associated with children's prosocial empathy. Respiratory sinus arrhythmia was measured in both caretakers and children (ages 9-14) and a continuous time series was estimated in order to maximize temporal resolution of physiological dynamics. Dyads varied in both the magnitude and direction of synchrony, with correlation coefficients ranging from moderately negative; r = -.44 (increases in one partner correspond to decreases in the other), to moderately positive; r = .55 (increases in one partner correspond to increases in the other). For both conditions, negative synchrony was correlated with higher child-reported empathy. For the negative mood condition, this effect was moderated by caretaker's emotional acceptance such that positive synchrony was associated with lower levels of empathy among dyads in which caretaker emotional acceptance was lower. These results suggest that positive physiological synchrony during a negative mood condition may not be adaptive for children when their caretaker lacks the skills to appropriately socialize their child's emotional development.

The Role of Children's Neurophysiological Functioning in the Links Between Emotion-Parenting Behaviors and Child Anxiety Symptoms: A Biological Sensitivity to Context Framework.

A plethora of data supports links between parenting behaviors and child anxiety, but few studies have examined factors that can contribute to variability in these relations. Adopting a biological sensitivity to context framework, this study explored the role of children's physiological stress reactivity in the links between emotion-parenting and child anxiety symptoms in a group of Chinese families. Sixty-one parent-child dyads (child Mage  = 8.21 years, SD = 1.40, range = 6-12 years) participated in an acute stress protocol, from which children's physiological (cortisol and respiratory sinus arrhythmia) responses to a social speech task were recorded. Participants then completed questionnaires assessing parents' emotion-parenting behaviors and children's anxiety symptoms. Results showed that the relation between supportive emotion-parenting and child anxiety was stronger in the context of greater child RSA suppression to acute stress, such that children higher in RSA suppression exhibited lower anxiety symptoms when supportive emotion-parenting was higher than when it was lower. Thus, these findings supported the biological sensitivity to context model. No significant moderation effect was detected for cortisol reactivity or recovery. Instead, exploratory mediation analyses showed that supportive emotion-parenting was negatively related to child anxiety via greater cortisol recovery. There was also a significant indirect path where unsupportive emotion-parenting was related to blunted cortisol recovery, which in turn was associated with higher child anxiety. The results highlight the importance of coaching parents to respond in supportive ways to children's emotional expressions, particularly in the context of greater child reactivity, to help buffer against childhood anxiety symptoms.

Una plétora de datos respaldan las conexiones entre las conductas de crianza y la ansiedad infantil, pero pocos estudios han analizado los factores que pueden contribuir a la variabilidad en estas relaciones. Mediante la adopción de una sensibilidad biológica al marco del contexto, el presente estudio analizó el papel de la reactividad fisiológica al estrés de los niños en los vínculos entre las conductas de crianza emocional y los síntomas de ansiedad infantil en un grupo de familias chinas. Sesenta y una díadas padre-hijo (edad promedio de los niños = 8.21 años, desviación típica = 1.40, rango = 6-12 años) participaron en un protocolo de estrés agudo, del cual se registraron las respuestas fisiológicas de los niños (el cortisol y la arritmia sinusal respiratoria) a una tarea de habla social. Luego, los participantes contestaron cuestionarios que evaluaban las conductas de crianza emocional de los padres y los síntomas de ansiedad de los niños. Los resultados demostraron que la relación entre la crianza emocional comprensiva y la ansiedad de los niños fue más fuerte en el contexto de una mayor supresión de la arritmia sinusal respiratoria del niño ante el estrés agudo, de manera que los niños con mayor supresión de la arritma sinusal respiratoria demostraron menos síntomas de ansiedad cuando la crianza emocional comprensiva fue mayor que cuando fue menor. Por lo tanto, estos resultados respaldaron la sensibilidad biológica al modelo del contexto. No se detectó ningún efecto de moderación importante para la reactividad o recuperación del cortisol. En cambio, los análisis exploratorios de mediación demostraron que la crianza emocional comprensiva estuvo relacionada negativamente con la ansiedad de los niños mediante una mayor recuperación de cortisol. También hubo una vía indirecta significativa donde la crianza emocional incomprensiva estuvo relacionada con la recuperación disminuida de cortisol, que a su vez estuvo asociada con una mayor ansiedad infantil. Los resultados destacan la importancia de capacitar a los padres para responder de maneras comprensivas a las expresiones emocionales de los niños, particularmente en el contexto de una mayor reactividad infantil, a fin de contribuir a atenuar los síntomas de ansiedad en la niñez.

Enhanced electrochemiluminescent brightness and stability of porphyrins by supramolecular pinning and pinching for sensitive zinc detection.

Ultrasensitive electrochemiluminescence (ECL) detection can benefit substantially from the rational configuration of emitter-enhancer stereochemistry. Here, using zinc(II) meso-5,10,15,20-tetra(4-sulfonatophenyl)porphyrin (ZnTSPP) as a model, we demonstrate that both the ECL intensity and the photostability of this emitter were significantly improved when it was trapped in pyridyl-bridged ß-cyclodextrin dimer (Py(CD)2); a synthetic enhancer that is ECL inactive. Through NMR characterization, we confirmed that ZnTSPP formed a clam-like inclusion complex involving pinning and pinching forces from the biocompatible container Py(CD)2. Up to a threefold increase in the ECL brightness of ZnTSPP was witnessed when it was encapsulated in ß-CD. Absorption and emission spectroscopic data revealed that both the extended excitation lifetime and the restricted mobility of the guest contributed to the observed improvement in signal transduction within the host molecule. This bioinspired entrapment also led to a marked boost in ECL stability. With the aid of the newly identified coreactant H2O2, the hollow TSPP@Py(CD)2 system was employed to create a Zn2+-selective probe that was capable of sensitive and accurate zinc detection. The observed increase in ECL conversion and enhanced photophysical properties of this compact supramolecular assembly render it a novel template for enhancing ECL in analytical applications. Graphical abstract ᅟ.

RSA reactivity in response to viewing bullying film and adolescent social adjustment.

This study assessed adolescent respiratory sinus arrhythmia (RSA) reactivity while watching a bullying film clip from Boyz'n the Hood. We emphasized the importance of considering measurement context of RSA and examined whether RSA reactivity moderated the links between environmental factors (i.e., neighborhood violence, parental psychological control, and parental acceptance) and adolescent social adjustment. Data were collected from 57 adolescents and their primary caregivers. Electrocardiogram (ECG) and respiration data were obtained from adolescents. Results indicated that neighborhood violence was positively associated with aggressive behavior but only among adolescents showing RSA augmentation, not suppression. Among youth displaying RSA suppression, parental acceptance was positively linked to prosocial behavior. We propose that, in the specific context of watching a bullying film clip, RSA suppression may relate to physiological regulation of emotion, which may differentially influence adolescents' responses to socialization.

Parent and child psychopathological symptoms: the mediating role of parental emotion dysregulation.

BACKGROUND: Parental psychopathological symptoms have been associated with a number of child psychological problems, yet little research has examined the role of parental emotion dysregulation on the intergenerational transmission of psychopathological symptoms. This study aims to examine the relationship between parents' and children's psychopathological symptoms with a focus on the mediating mechanism of parental emotion dysregulation on these relationships. METHODS: Eighty-nine Chinese parents and their school-age children between the ages of 7 and 12 (49 males, M age = 8.79, SD = 1.81) participated in the study. In the initial phase of the study, parents filled out a series of questionnaires reporting their own psychopathological symptoms via SCL-90 and difficulties with emotion regulation via Difficulties in Emotion Regulation Scale. After 9 months, the parents reported their children's internalizing and externalizing problems via Child Behavior Checklist, and the children self-reported anxiety symptoms via Screen for Child Anxiety Related Emotional Disorders in the second phase of the study. RESULTS: The results showed that parental emotion dysregulation played an important role as a mediator of the relationship between parental psychopathological symptoms and child internalizing problems and separation anxiety, which indicates that parents' mental health problems were significantly associated with their difficulties with emotion regulation, which in turn led to more internalizing problems and separation anxiety in their children. However, we did not find a mediating effect of parental emotion dysregulation on the links between parent psychopathology and child externalizing problems or other types of self-reported anxiety symptoms. CONCLUSIONS: Our findings highlighted the importance of implementing more psycho-education programs that specifically target parents' emotion regulation abilities in both community and clinical settings to ameliorate the intergenerational transmission of psychopathological symptoms between generations.

[Different doses of dexmedetomidine for quiet extubation during anesthesia recovery in hypertensive patients].

OBJECTIVE: To explore the feasibility of different doses of dexmedetomidine for quiet extubation during anesthesia recovery in hypertensive patients monitored with Narcotrend. METHODS: A total of 120 hypertensive patients scheduled for thyroid surgery from August 2012 to June 2014 in the Second Affiliated Hospital of Wenzhou Medical University, were randomly divided into 6 groups (n=20 each). Dexmedetomidine 0.4 (group M1), 0.6 (group M2), 0.8 (group M3) or 1.0 (group M4) µg·kg(-1)·h(-1), remifentanil 0.1 µg·kg(-1)·min(-1) (group R) and normal saline (group S) were infused for half an hour before the end of surgery, and extubation was carried out when Narcotrend index (NI) values were ≥80 in each group. Data of heart rate (HR), systolic blood pressure (SBP) and minimal alveolar concentration (MAC) of sevoflurane were observed and recorded at the time of baseline (T0), half an hour (T1) and 15 min (T2) before the end of surgery, stopping sevoflurane (T3), before extubation (T4), 1 min (T5), 5 min (T6) and 10 min (T7) after extubation. Extubation time, recovery time and related adverse reactions were also recorded. RESULTS: Compared with T0, SBP and HR at T4 to T7 in four M groups were significantly lower (all P<0.05). SBP and HR at T6, T7 in group R were significantly lower than at T0 (all P<0.05). SBP at T6, T7 and HR at T4 to T7 in group R were significantly lower than that of group S (all P<0.05). SBP and HR at T4 to T7 in four M groups were significantly lower than that of group S and group R (all P<0.05). The values of MAC of sevoflurane at T2 and T3 in group R and M2-4 were significantly lower than at T1 and that of group S (all P<0.05). Recovery time in group M3 [(19.1±2.8) min] and group M4 [(20.6±4.1) min] were significantly longer compared with other four groups (all P<0.05). The percentage of cough grade at level I and II in each M group and group R during extubation was significantly higher than that in group S (85%, 85%, 90%, 95%, 80% vs 45%, all P<0.05). CONCLUSIONS: Dexmedetomidine could be safely used in hypertensive patients monitored with Narcotrend for quiet extubation during anesthesia recovery, but larger doses of dexmedetomidine may prolong the recovery time.

Within-person bidirectional associations between maternal cortisol reactivity and harsh parenting across infancy and toddlerhood.

To understand transactional associations between mothers' biological stress responsivity and parenting behaviors, we examined bidirectional effects between maternal cortisol reactivity to observing their children during distress-eliciting paradigms and harsh parenting across infancy and toddlerhood using longitudinal data from the Family Life Project (N = 1,292, 41.5% African American). Children completed a series of distress-eliciting laboratory paradigms when they were 7, 15, and 24 months old, and mothers observed their children during the paradigms. Maternal cortisol reactivity was computed as a residualized change score from baseline to 20 min postparadigm, controlling for the time of day the saliva sample was collected. Harsh parenting was measured using five items from the Home Observation Measurement of the Environment inventory. A random-intercept cross-lagged panel model revealed that increases in maternal cortisol reactivity at 15 months predicted subsequent increases in harsh parenting at 24 months. Similarly, increases in harsh parenting at 15 months predicted increases in maternal cortisol reactivity at 24 months. Findings indicate that increased cortisol reactivity to children's distress in early toddlerhood may indicate a risk for harsh parenting in late toddlerhood and that increases in harsh parenting can also negatively impact mothers' stress physiology over time. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

[Occurrence Characteristics of Microplastics in Multi-environmental Media and Bellamya aeruginosa of Manao River].

Microplastic pollution poses threats to aquatic ecosystems and human health. In this study, in order to investigate the characteristics of microplastic occurrence in different environmental media, the abundance, particle size, shape, color, and composition types of microplastics in the water column, sediment, riparian zone soil, and the benthic snail Bellamya aeruginosa of the Manao River were analyzed using field sampling, microscopic observation, and Fourier infrared spectroscopy. The results showed that the average abundance of microplastics in the surface water of the Manao River was (5.9±0.26) n·L-1; the abundance of microplastics in the upper sediment (by dry weight) was (1.35±0.1) n·g-1, and that in the lower sediment (by dry weight) was (0.93±0.12) n·g-1. The abundance of microplastics in the near riparian zone soil (by dry weight) was (0.68±0.16) n·g-1, and that in the far riparian zone soil (by dry weight) was (0.69±0.14) n·g-1, and the abundance of microplastics in the B. aeruginosa was (2.06±0.25) n·g-1. The analysis results showed that the abundance of microplastics in the upper and lower sediments were positively correlated; the abundance of microplastics in B. aeruginosa was positively correlated with the abundance of microplastics in the upper and lower sediments, respectively; and the abundance of microplastics in the near and far riparian zone soils were also correlated. Most of the microplastics within each environmental medium and B. aeruginosa were <0.1 mm in size, mainly in the form of fibers and fragments, mainly blue and black in color, and mainly composed of polypropylene (PP) and polyethylene (PE). It was found that microplastics in riparian zone soils mainly originated from the fragmentation and decomposition of agricultural plastic films. The results of this study shed light on the accumulation of microplastics in macrobenthic organisms through the investigation of microplastics in multi-environmental media and in the B. aeruginosa, which helps us to understand the potential ecological risk of microplastics in a comprehensive manner.

Protective effect of dexmedetomidine against delayed bone healing caused by morphine via PI3K/Akt mediated Nrf2 antioxidant defense system.

Background: As a class of analgesics, opioids are frequently used to treat both acute and chronic moderate to severe pain. Patients frequently receive opioid painkillers after orthopedic accidents or surgeries. Evidence suggests that opioid drug users have a 55.1% higher risk of fracture and poor bone repair than non-users of opioid drugs. The key pathogenic alterations in the incidence and progression of poor bone repair are over apoptosis and aging of osteoblasts due to the stress caused by oxidation. Dexmedetomidine (Dex) has been proven to protect against a variety of degenerative illnesses by reducing oxidative stress. However, nothing is known about how it affects bone repair. Methods: PI3K/Akt/Nrf2 pathway was detected by immunofluorescence and Western blot. SOD, CAT, JC-1, dihydroethidium and mitosox were used in the Oxidative Stress. Micro-CT, H&E and Masson's staining, immunohistochemically were performed to evaluate the therapeutic effects of DEX on calvarial defects in the morphine-induced rat model. Results: We found that morphine-induced an imbalance in the metabolism and catabolism of primary rat Osteoblasts. However, these conditions could be inhibited by DEX treatment. In the meantime, DEX induced the expression of Nrf2-regulated antioxidant enzymes such as NQO1, HO-1, GCLm, GCLc, and TrxR1. DEX-mediated Nrf2 activation is linked to the PI3K/Akt signaling system. Furthermore, it has been established that intravenous DEX enhanced the growth of bone healing in a model of a surgically produced rat cranial lesion. Conclusion: This is the first description of the unique DEX mechanism acting as a Nrf2 activator against morphine-mediated oxidative harm, raising the possibility that the substance may be used to prevent bone defects.

20- Deoxyingenol attenuate morphine-induced hippocampus neurotoxicity and memory impairments in rats.

Objective: The present study aimed to see if 20-Deoxyingenol(20-DOI) could protect hippocampus neurons from the neurotoxic effects of morphine and reduce memory loss in rats. Method: Male Wistar rats were given morphine hydrochloride (45 mg/kg, sc, four weeks) and 20-DOI (10, 20 mg/kg, ip., coadministered with morphine) for the Morris Water Maze (MWM) test to investigate the effects of 20-DOI on spatial learning and memory. Western blotting was used to evaluate the expression of the hippocampal CA1 region of the cleaved caspase-3, Bax, and Bcl2 proteins and so on. Moreover, these assays were used to evaluate the expression of superoxide dismutase (SOD)2, heme oxygenase 1(HO1) protein, and glutathione peroxidase (GPx) activity within the hippocampus CA1 area. Results: The administration of 20-DOI (10 and 20 mg/kg) to morphine-treated mice enhanced spatial learning and reduced memory deficits. Additionally, 20-DOI treatment reduced apoptosis and oxidative stress in the hippocampal CA1 region of morphine-treated rats. Moreover, 20-DOI improved the autophagy level of the hippocampal CA1 area of morphine-treated rats using Transcription factor EB (TFEB), and 20-DOI prevented spatial learning and memory impairment in morphine-treated rats. The current observation could be partially due to the inhibition of neuronal apoptosis and oxidative stress in the hippocampal CA1 region of rats treated with morphine and the improved autophagy in this region. Conclusions: 20-DOI attenuated morphine administration in rats with chronic disease caused spatial learning and memory dysfunction. These mechanistic effects could be partially related to 20-DOI protecting the CA1 region of rat hippocampal neurons from the morphine-induced oxidative stress, apoptosis, and autophagy through TFEB.