RESUMEN
Objective: To explore the relationship between the hepatic caudate lobe boundary and the ductal system so as to guide the identification of the anatomical relationship during liver surgery. Methods: The specific parts were observed and the liver parenchyma was removed according to 41 cadaveric liver autopsy specimens. The critical relationship between the hepatic caudate lobe and other ducts was observed to explore the reticular duct structure. Results: The plane formed by the hepatic hilar plate and Arantius ligament served as the boundary between the caudate lobe and other hepatic lobes. The caudate lobe hepatic portal vein was composed of numerous small branches from its left and right branches. The portal vein adjacent to the vena cava was mainly derived from the left branch, and to a lesser extent from the right branch. Blood was drained straight from the caudate lobe vein into the inferior vena cava via the short hepatic vein. There were three or four bile duct branches in the caudate lobe. The main source of arterial blood flow were the left and right branches of the hepatic artery. An avascular zone of loose connective tissue was found between the caudate lobe and the retrohepatic inferior vena cava. Conclusion: The hepatic caudate lobe is an independent lobe. During hepatic caudate lobe surgery, the plane formed by the hepatic hilar plate and Arantius ligament can serve as the boundary between the caudate lobe and other hepatic lobes and be used for anatomical site identification. The duct system of the caudate lobe's is complicated, but it also has its own distinct regularity.
Asunto(s)
Arteria Hepática , Hígado , Humanos , Hígado/irrigación sanguínea , Conductos Biliares , Vena Porta , Venas Hepáticas , HepatectomíaRESUMEN
A*33:03:13 differs from A*33:03:01 by one nucleotide change at nucleotide 714 in exon 4 from A to G.
Asunto(s)
Alelos , Pueblo Asiatico/genética , Antígenos HLA-A/genética , Adulto , Secuencia de Bases , China , Exones/genética , Familia , Femenino , Humanos , Datos de Secuencia Molecular , Alineación de SecuenciaRESUMEN
We investigated the distribution of human leukocyte antigen (HLA)-B27 subtypes in Uygur ankylosing spondylitis patients in Xinjiang. B27-positive patients with ankylosing spondylitis were subtyped by using polymerase chain reaction-sequence-based typing. The HLA-B27 subtype frequencies of Uygur patients were compared with those in Han patients in Xinjiang and the other areas of China. B*2705 was the predominant subtype in Uygur patients with a frequency of 58.95%, which was much higher than that in Han patients in Xinjiang (31.58%, P < 0.05) and the other areas of China (excluding the Shandong region, which was 63.89%). The frequency of B*2704 (27.37%) in Uygur patients was the lowest and significantly lower than that in Han patients (61.18%, P < 0.05) and in 8 other areas of China. B*2710 has not been previously reported in Uygur ankylosing spondylitis patients; B*2704 was the main (61.18%) subtype in Han patients in Xinjiang, followed by B*2705 (31.58%) and was similar to the characteristics of Han patients in the other areas of China. B*2724 in Han ankylosing spondylitis patients has not been previously reported. Additionally, the B*2702/B*2705 homozygote was identified in Uygur patients. B*2702/B*2704, B*2704/B*2705, and B*2705/B*2705 homozygotes were identified in 3 Han patients. The distribution of HLAB27 subtypes in Uygur ankylosing spondylitis patients in Xinjiang significantly differed from that in Han patients. Understanding the distribution of HLAB27 subtypes in ethnic minority populations of Xinjiang is important for anthropological genetic studies and for analyzing the impact of genetic background on ankylosing spondylitis susceptibility.
Asunto(s)
Predisposición Genética a la Enfermedad , Antígeno HLA-B27/genética , Espondilitis Anquilosante/genética , Adolescente , Adulto , Anciano , Alelos , Pueblo Asiatico/genética , China , Etnicidad , Femenino , Humanos , Masculino , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Espondilitis Anquilosante/patologíaRESUMEN
We investigated the presence of serum antinuclear antibodies (ANAs) and autoantibodies and their relationship with serum prognostic indicators in lymphoma patients. The study population comprised 127 patients diagnosed with lymphoma and 138 healthy control subjects. The blood samples of the participants were assayed for ANAs by immunofluorescence, and autoantibodies were detected by western blotting. Serum ANAs were detected in 31.5 (40/127) and 6.5% (9/138) of lymphoma patients and control subjects, respectively. There was a statistically significant difference between the lymphoma and the control groups (P < 0.05). The level of lactate dehydrogenase in the ANA-positive subjects was significantly lower than in the ANA-negative subjects (P < 0.05). Low ANA titers (1:100) were commonly found in the ANA-positive subjects and the control subjects, and the fluorescence models were diverse. Autoantibodies were found in 35% (14/40) of the ANA-positive patients by western blotting. Detection of ANAs in lymphoma patients helps in determining the diagnosis and prognosis of lymphoma, but has no independent diagnostic value; there are still various autoantibodies of unknown significance that require further study.
Asunto(s)
Anticuerpos Antinucleares/sangre , Biomarcadores de Tumor/sangre , Linfoma/sangre , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Niño , Femenino , Humanos , L-Lactato Deshidrogenasa/sangre , Masculino , Persona de Mediana EdadRESUMEN
The novel HLA-B*51:144 allele shows one nucleotide difference from B*51:02:02 in exon 3.
Asunto(s)
Alelos , Exones , Antígenos HLA-B/genética , Pueblo Asiatico , HumanosRESUMEN
A*02:01:79 is identical to A*02:01:01:01, except for one nucleotide change at nt 762 in exon 4 from C to T.
Asunto(s)
Alelos , Exones/genética , Antígeno HLA-A2/genética , Mutación/genética , Secuencia de Bases , China , Codón/genética , Genoma Humano , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
The aim of this study was to examine the interleukin-1B (IL-1B) gene promoter region -31 (IL-1B-31) polymorphism distribution characteristic of Hakka gastric cancer patients in Guangdong Province and to explore its association with gastric cancer. We used the 1:1 case-control method, matrix-assisted laser desorption ionization flight time mass spectrometry, and MassARRAY-IPLEX technology to genotype IL-1B-31 (-31C> T) in 52 Hakka gastric cancer patients and 52 Hakka control subjects in Meizhou. Three genotypes - CT, TT, and CC - of IL-1B-31 were found in the Meizhou Hakka population. Their distribution frequencies in the gastric cancer group were 40.38, 40.38, and 19.23%, respectively, whereas the frequencies in control subjects were 57.69, 17.31, and 25.00%, respectively. The differences in frequency distributions of the genotypes between the 2 groups were statistically significant (chi-square = 6.78, P < 0.05). Subjects with the TT genotype had a higher risk of gastric cancer compared with that in subjects carrying the CT genotype (odds ratio = 2.857, 95% confidence interval = 1.114-7.328). This risk was more apparent in male subjects. IL-1B-31 locus polymorphism may be associated with gastric cancer susceptibility in this population, but additional studies with larger sample size are needed to confirm the conclusions.
Asunto(s)
Interleucina-1beta/genética , Polimorfismo de Nucleótido Simple , Neoplasias Gástricas/genética , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Alelos , Estudios de Casos y Controles , China/epidemiología , Femenino , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Riesgo , Factores Sexuales , Neoplasias Gástricas/epidemiologíaRESUMEN
The aim of this study was to investigate the characteristics and polymorphisms of the T-cell receptor BV complementarity-determining region 3 (TCR BV CDR3) gene in peripheral blood mononuclear cells (PBMCs) from patients with uveitis to provide an experimental basis for studying the pathogenesis of this disease. RT-PCR amplification of 26 subfamilies of the TCR BV CDR3 gene and immune spectratyping analysis were used to study the pedigree drift of TCR BV CDR3 in PBMCs from the uveitis patients. The following results were obtained: 1) the vast majority of the TCR BV CDR3 spectra in PBMCs in 5 healthy subjects fit the normal (or Gaussian) distribution. The distributions of the TCR BV CDR3 spectra in 4 patients with uveitis were non-normal and showed an abnormal peak including a widowed peak trend, a partial peak, and an irregular abnormal peak. 2) In the 26 TCR BV subfamilies, the abnormal peak frequency was different in the various subfamilies. The BV2 and BV17 (both 3/4) subfamilies had higher frequencies of the non-normally distributed abnormal peak. The BV5.2, BV6, BV15, and BV18 subfamilies showed no abnormal peaks. 3) TCR BV2 and BV17 yielded an abnormal peak in 3 HLA-B27-negative patients; however, no such abnormalities were detected in HLA-B27-positive patients. The abnormal expression of some TCR BV subfamilies in PBMCs from patients with uveitis may be associated with the immune pathogenesis of the disease. Our study provides the basis for further investigations into the pathogenesis of uveitis.
Asunto(s)
Regiones Determinantes de Complementariedad/genética , Leucocitos Mononucleares/metabolismo , Receptores de Antígenos de Linfocitos T alfa-beta/genética , Uveítis/sangre , Uveítis/inmunología , Adulto , Femenino , Variación Genética , Humanos , Leucocitos Mononucleares/inmunología , Masculino , Persona de Mediana Edad , Uveítis/genética , Adulto JovenRESUMEN
HLA-DQB1*03:39 is identical to HLA-DQB1*03:03:02, except for one nucleotide change at nt 477 (CâG) in exon 3.
Asunto(s)
Alelos , Cadenas beta de HLA-DQ/genética , Polimorfismo de Nucleótido Simple , Pueblo Asiatico , Secuencia de Bases , Trasplante de Médula Ósea , Exones , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de Secuencia , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
Exons 2, 3 and 4 of HLA-B*40:06:06 allele are identical to those of HLA-B*40:06:01:01, except for one nucleotide change.
Asunto(s)
Alelos , Exones , Antígenos HLA-B/genética , Mutación , Pueblo Asiatico , Secuencia de Bases , Codón , Antígenos HLA-B/inmunología , Trasplante de Células Madre Hematopoyéticas , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de Secuencia , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
The full-length sequences of exons 1-8 of this novel HLA-A*02:230 allele are identical to those of HLA-A*02:03:01, except for one nucleotide change at nt 858 in exon 3 from G to A.
Asunto(s)
Alelos , Exones/genética , Antígeno HLA-A2/genética , HumanosRESUMEN
Exons 1-8 of HLA-A*24:132N allele are identical to those of HLA-A*24:02:01:01, except for one nucleotide change at nt 746 in exon 3 from C to A.
Asunto(s)
Antígenos HLA-A/genética , Antígeno HLA-A24/genética , Mutación Puntual , Polimorfismo de Nucleótido Simple , Alelos , Secuencia de Aminoácidos , Pueblo Asiatico , Secuencia de Bases , Trasplante de Médula Ósea , Codón de Terminación , Exones , Antígenos HLA-A/inmunología , Antígeno HLA-A24/inmunología , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Donantes de TejidosRESUMEN
The novel HLA-C*04:107 allele shows one nucleotide difference from C*04:03 in exon 2 at nucleotide position 341.
Asunto(s)
Alelos , Antígenos HLA-C/genética , Polimorfismo de Nucleótido Simple , Adulto , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Pueblo Asiatico , Secuencia de Bases , Codón , Exones , Antígenos HLA-C/inmunología , Trasplante de Células Madre Hematopoyéticas , Prueba de Histocompatibilidad , Humanos , Masculino , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Donantes de TejidosRESUMEN
The sequence of the novel allele is identical to HLA-DRB1*16:02:01 except for one nucleotide change at nt203 (GâA), resulting in a coding change, 39 R (CGC)âH (CAC).
Asunto(s)
Alelos , Médula Ósea/inmunología , Cadenas HLA-DRB1/genética , Nucleótidos/genética , Sustitución de Aminoácidos , Pueblo Asiatico , Secuencia de Bases , Exones , Sitios Genéticos , Cadenas HLA-DRB1/inmunología , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Isoformas de Proteínas/genética , Isoformas de Proteínas/inmunología , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
A novel HLA-C allele, Cw*0824, which was identified from an individual of the Han Chinese, differs from Cw*080101 at codon 222 (GAG > AAG ) in exon 4, which results in an amino acid change Glu222Lys.
Asunto(s)
Alelos , Pueblo Asiatico , Antígenos HLA-C/genética , Sustitución de Aminoácidos , Secuencia de Bases , Exones , Humanos , Datos de Secuencia MolecularRESUMEN
The novel human leukocyte antigen (HLA)-Cw*0348 allele was identified by sequence-based typing in a Chinese family. This allele shows that the sequences of exons 1-3 of HLA-Cw*0348 are identical to those of HLA-Cw*030401 except for a nucleotide substitution that changes CCG to CTG at codon 57, resulting in an amino acid change from Pro to Leu in the protein, and this is a unique nucleotide change among the HLA-C alleles, suggesting a point mutation mechanism. The extended haplotype carrying the new allele was deduced from the family group typing and defined as A*110101, B*1301, Cw*0348, DRB1*0405, and DQB1*0402.
Asunto(s)
Sustitución de Aminoácidos/genética , Pueblo Asiatico/genética , Antígenos HLA-C/genética , Adulto , Alelos , Secuencia de Bases , Exones/genética , Humanos , Masculino , Datos de Secuencia Molecular , Linaje , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
This allele is characterized by a nucleotide substitution (C>T) in exon 4 at position nt 672, codon 200 (ACC>ACT), no coding change.
Asunto(s)
Pueblo Asiatico/genética , Antígenos HLA-A/genética , Alelos , Secuencia de Bases , Análisis Mutacional de ADN , Humanos , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple , Homología de Secuencia de Ácido NucleicoRESUMEN
The full-length sequences of exons 1-8 of this novel HLA-Cw*070205 allele are identical to those of HLA-Cw*07020101, except for one nucleotide change at nt 498 in exon 3 from C to T, which result in a synonymous amino acid substitution from ATC to ATT at codon 142 in exon 3.
Asunto(s)
Antígenos HLA-C/genética , Alelos , Sustitución de Aminoácidos , Pueblo Asiatico/genética , Secuencia de Bases , Humanos , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
A novel human leukocyte antigen (HLA)-B*40 allele, officially named B*4081, was identified during routine high-resolution sequence-based typing in a Chinese potential hematopoietic stem cell transplantation donor. The HLA-B*4081 allele shows one nucleotide difference from B*400101 in exon 2 at nucleotide position 124 where G-->C (codon 18 GGG-->CGG) resulting in a coding change, 18Gly is changed to Arg, this is a unique nucleotide change among the HLA class I alleles, suggesting a point mutation mechanism.
Asunto(s)
Alelos , Antígenos HLA-B/genética , Adulto , Sustitución de Aminoácidos/genética , Secuencia de Bases , China , Exones/genética , Antígeno HLA-B40 , Trasplante de Células Madre Hematopoyéticas , Humanos , Donadores Vivos , Masculino , Datos de Secuencia Molecular , Mutación Puntual/genética , Alineación de SecuenciaRESUMEN
We report here a novel human leukocyte antigen-DRB1 allele, DRB1*112802, which was identified from a Chinese individual during sequence-based typing. The new allele is identical to DRB1*112801 except for one nucleotide change at nucleotide 189 (A --> G ), codon 34 Q (CAA) --> Q (CAG), no coding change.