Dietary supplement use among dietetics students at the University of KwaZulu-Natal.

BACKGROUND: A dietary supplement is a product that aims to add nutritional value to the diet. University students are known to make use of dietary supplements to improve their academic performance, increase energy levels and promote overall general health. Based on assumption, students studying towards a nutrition-related degree may eat healthily and choose not to use dietary supplements. Alternatively, because of their interest in and exposure to nutrition, they may decide to use dietary supplements. However, there is a lack of published studies investigating the prevalence of dietary supplement use and reasons for use among South African university students studying towards a nutrition-related degree. AIM: The aim of this study was to assess the use of dietary supplements by dietetics students. SETTING: University of KwaZulu-Natal (UKZN). METHODS: A cross-sectional, descriptive study was conducted using a self-administered questionnaire. RESULTS: Of the 139 participants, 23% (n = 32) used dietary supplements. There was a greater use by female students, those who lived at home and those registered for the Postgraduate Diploma in Dietetics. Reasons for using dietary supplements included the following: to strengthen the immune system (62.5%), to improve energy levels (56.3%) and to enhance physical health (50%). Cost (32.7%; n = 35), an adequate diet (22.4%; n = 24) and not necessary or waste of money (15%; n = 16) were reasons for not using dietary supplements. Most students (84.4%) made use of a multivitamin and mineral supplement. CONCLUSION: There was a low prevalence of dietary supplement use by UKZN dietetics students, with the high cost of supplements given as the main reason for non-use.

Nephrotoxicity and highly active antiretroviral therapy: Mitigating action of Momordica charantia.

Momordica charantia (M. charantia) is known for its antioxidant and antidiabetic properties. The aim of this study is to investigate the renoprotective effects of M. charantia in rats following treatment with highly active antiretroviral therapy (HAART) regimen triplavar. Adult male Sprague-Dawley rats weighing 178.1-220.5 g (n = 36) were divided into six groups (A-F) with each group comprising of six (n = 6) rats. The drugs and extract were administered via oral gavage. The therapeutic dose of triplavar was adjusted using the human therapeutic dose equivalent for the rat model. Animals were euthanized on the tenth week with kidneys removed for examination and blood obtained via cardiac puncture. Levels of oxidative stress enzymes (superoxide dismutase-SOD, catalase-CAT, and reduced glutathione-GSH) were significantly lowered in all groups not receiving M. charantia. The levels of thiobarbituric acid reactive substances (TBARS) were increased resulting in free radical formation via auto-oxidation. Renal parameters showed no albuminuria, normal blood urea nitrogen (BUN), serum creatinine (SCr) and electrolytes in groups treated with M. charantia. HAART treated (Group B) showed severe albuminuria, a significantly (p < 0.05) raised BUN and SCr and gross electrolyte disturbances. Blood glucose levels were significantly raised in groups not receiving the adjuvant M. charantia (p < 0.05). Histopathology in HAART treated animals showed glomerular capillary abnormalities and cellular infiltrations while M. charantia treated animals showed an essentially normal glomerular appearance with capillary loops and normal cytoarchitecture. In conclusion M. charantia extract administration improved blood glucose levels, restored renal histology, reinstate renal function, reduce body weight loss and restores hyperglycemia.

Isolation and characterization of antimicrobial constituents of Searsia chirindensis L. (Anacardiaceae) leaf extracts.

ETHNOPHARMACOLOGICAL RELEVANCE: Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant. MATERIALS AND METHODS: The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses. RESULTS: Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: myricetin-3-O-arabinopyranoside (2), myricetrin-3-O-rhamnoside (3), kaempferol-3-O-rhamnoside (4) and quercetin-3-O-arabinofuranoside (5). These compounds are reported from Searsia chirindensis for the first time. All the compounds showed good antibacterial activity against all bacterial strains tested. Their minimum inhibitory concentrations ranged from 30 to 250 µg/mL. CONCLUSIONS: Antibacterial activity demonstrated by the extracts and isolated compounds provides credence to the ethnomedicinal use of Searsia chirindensis against diarrhoea.