RESUMEN
The ultrasonic cell disruption method was used to efficiently extract isothiocyanates and other volatile compounds from radish microgreens. A total of 51 volatiles were identified and quantified by headspace solid-phase micro-extraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS) in four radish microgreen cultivars, mainly including alcohols, aldehydes, isothiocyanates, sulfides, ketones, esters, terpenes, and hydrocarbons. The correlation between cultivars and volatile compounds was determined by chemometrics analysis, including principal component analysis (PCA) and hierarchical clustering heat maps. The aroma profiles were distinguished based on the odor activity value (OAV), odor contribution rate (OCR), and radar fingerprint chart (RFC) of volatile compounds. This study not only revealed the different flavor characteristics in four cultivars but also established a theoretical basis for the genetic improvement of radish microgreen flavors.
Asunto(s)
Raphanus , Compuestos Orgánicos Volátiles , Microextracción en Fase Sólida/métodos , Cromatografía de Gases y Espectrometría de Masas/métodos , Ultrasonido , Compuestos Orgánicos Volátiles/análisis , Odorantes/análisis , Isotiocianatos/análisisRESUMEN
Radish microgreens constitute a good source of bioactive compounds; however, they are very delicate and have a short shelf life. In this study, we investigated the impact of light exposure and modified atmosphere packaging on sensorial quality, bioactive compound concentrations and antioxidant capacity of radish microgreens during storage. Results showed that light exposure during storage increased the amount of ascorbic acid and had no effect on α-tocopherol or total phenolic concentrations. Dark storage resulted in higher hydroxyl radical scavenging capacity and carotenoid retention. No significant differences were found for relative 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity between light and dark treatments. Radish microgreens in bags of 29.5 pmol s(-1) m(-2) Pa(-1) oxygen transmission rate (OTR) maintained better quality than those within laser microperforated bags. In conclusion, light exposure accelerated deterioration of radish microgreens, while dark storage maintained quality; and application of OTR bags was beneficial in extending shelf life.