RESUMO
The aim of the study was to determine polyamine concentration in erythrocytes in the blood of pregnant women with intrauterine growth retardation of different severity. The study included 100 pregnant women (from 23 to 40 weeks of gestation). The main group consisted of 80 pregnant women with intrauterine growth retardation. The control group consisted of 20 women with physiological course of pregnancy. The patients of the main group were divided into three clinical groups regarding intrauterine growth retardation staging. Group I included 38 pregnant women with stage I IUGR, 22 pregnant women with stage II IUGR were in group II and 20 pregnant women with stage III IUGR - in group III.Polyamine concentration in erythrocytes in the blood of pregnant women with intrauterine growth retardation was determined by using Agilent 1200 series (USA) high performance liquid chromatography [4]. The standards of polyamine hydrochlorides were obtained from Sigma-Aldrich Company (USA). The variational methods were used to make the statistical analysis of outcomes by standard licensed computer programs: STATISTICA 6.0, Microsoft Excel, ANOVA «Statistica¼. The study results were presented in the form of M±m and differences were considered reliable at Ñ<0,05 by Student's t-criterion. The conducted study has revealed that polyamine concentration in erythrocytes in the blood of pregnant women with intrauterine growth retardation is drastically lower if compared with pregnant women with physiological course of pregnancy. At the same time the putrescine concentration is higher, andspermidineandspermine concentrations are significantly reduced in the pregnant women with intrauterine growth retardation in comparison with the control group.According to the obtained results the polyamine exchange proves to be disturbed in pregnant women with intrauterine growth retardation. The progression of polyamine imbalance depends on the severity of fetal growth retardation in pregnant women.