Biases in Ecoacoustics Analysis: A Protocol to Equalize Audio Recorders.

Eco-acoustic indices allow us to rapidly evaluate habitats and ecosystems and derive information about anthropophonic impacts. However, it is proven that indices' values and trends are not comparable between studies. These incongruences may be caused by the availability on the market of recorders with different characteristics and costs. Thus, there is a need to reduce these biases and incongruences to ensure an accurate analysis and comparison between soundscape ecology studies and habitat assessments. In this study, we propose and validate an audio recording equalization protocol to reduce eco-acoustic indices' biases, by testing three soundscape recorder models: Song Meter Micro, Soundscape Explorer Terrestrial and Audiomoth. The equalization process aligns the signal amplitude and frequency response of the soundscape recorders to those of a type 1 level meter. The adjustment was made in MATLAB R2023a using a filter curve generated comparing a reference signal (white noise); the measurements were performed in an anechoic chamber using 11 audio sensors and a type 1 sound level meter (able to produce a .WAV file). The statistical validation of the procedure was performed on recordings obtained in an urban and Regional Park (Italy) assessing a significant reduction in indices' biases on the Song Meter Micro and Audiomoth.

Mapping of the Acoustic Environment at an Urban Park in the City Area of Milan, Italy, Using Very Low-Cost Sensors.

The-growing influence of urbanisation on green areas can greatly benefit from passive acoustic monitoring (PAM) across spatiotemporal continua to provide biodiversity estimation and useful information for conservation planning and development decisions. The capability of eco-acoustic indices to capture different sound features has been harnessed to identify areas within the Parco Nord of Milan, Italy, characterised by different degrees of anthropic disturbance and biophonic activity. For this purpose, we used a network of very low-cost sensors distributed over an area of approximately 20 hectares to highlight areas with different acoustic properties. The audio files analysed in this study were recorded at 16 sites on four sessions during the period 25-29 May (2015), from 06:30 a.m. to 10:00 a.m. Seven eco-acoustic indices, namely Acoustic Complexity Index (ACI), Acoustic Diversity Index (ADI), Acoustic Evenness Index (AEI), Bio-Acoustic Index (BI), Acoustic Entropy Index (H), Normalized Difference Soundscape Index (NSDI), and Dynamic Spectral Centroid (DSC) were computed at 1 s integration time and the resulting time series were described by seven statistical descriptors. A dimensionality reduction of the indices carrying similar sound information was obtained by performing principal component analysis (PCA). Over the retained dimensions, describing a large (∼80%) variance of the original variables, a cluster analysis allowed discriminating among sites characterized by different combination of eco-acoustic indices (dimensions). The results show that the obtained groups are well correlated with the results of an aural survey aimed at determining the sound components at the sixteen sites (biophonies, technophonies, and geophonies). This outcome highlights the capability of this analysis of discriminating sites with different environmental sounds, thus allowing to create a map of the acoustic environment over an extended area.