Towards eco-friendly biocides: preparation, antibiofilm activity of hemibastadin analogues.

The antibiofilm activity of three hemibastadins analogues was evaluated against different marine bacterial strains through mono-species biofilms and through a multi-species model of biofilm. Results showed that compound 3 exhibited interesting antibiofilm efficiencies effective concentrations (EC50 ) in the range of 30-100 µmol l-1 without acute toxicity against bacteria. Toxicity against nontargeted organisms was also considered showing that the compound did not affect the global bacterial community at a concentration of 75-100 µmol l-1 . These results provided baseline data concerning the toxicity of antibiofilm biocides against marine organisms. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports relevant information about antibiofilm activity of original derivatives of hemibastadin alkaloids. The most active compound was shown to act as a specific anti-biofilm inhibitor without affecting viability of the targeted bacteria no more than those of the global bacterial community of a seawater sample. Taken together, these findings indicate the potentiality of such compounds to be used as original nonbiocidal molecules for designing eco-friendly antifouling solutions.

Towards smart biocide-free anti-biofilm strategies: Click-based synthesis of cinnamide analogues as anti-biofilm compounds against marine bacteria.

A set of triazole-based analogues of N-coumaroyltyramine was designed to discover potential leads that may help in the control of bacterial biofilms. the most potent compounds act as inhibitors of biofilm development with EC50 closed to ampicillin (EC50 = 11 µM) without toxic effect on bacterial growth even at high concentrations(100 µM).

Click-based synthesis of bromotyrosine alkaloid analogs as potential anti-biofilm leads for SAR studies.

A library of triazole-based analogs of bromotyramine alkaloids such as verongamines, hemibastadins, pseudoceramine D and clavatidine E was designed in order to identify promising leads that may help in the control of bacterial biofilms. Twenty-three compounds were screened for their biofilm inhibitory activity against three strains of Gram-negative bacteria. SAR studies revealed that hemibastadins analogs were the most active compounds which act as inhibitors of biofilm development (EC50 8.8-29µM) without effect on bacterial growth even at high concentrations (100µM).

Effects of height and load weight on shoulder muscle work during overhead lifting task.

Few musculoskeletal models are available to assess shoulder deeper muscle demand during overhead lifting tasks. Our objective was to implement a musculoskeletal model to assess the effect of lifting height and load on shoulder muscle work. A musculoskeletal model scaled from 15 male subjects was used to calculate shoulder muscle work during six lifting tasks. Boxes containing three different loads (6, 12 and 18 kg) were lifted by the subjects from the waist to shoulder or eye level. After optimisation of the maximal isometric force of the model's muscles, the bio-fidelity of the model was improved by 19%. The latter was able to reproduce the subjects' lifting movements. Mechanical work of the rotator cuff muscles, upper trapezius and anterior deltoid was increased with lifting load and height augmentation. In conclusion, the use of a musculoskeletal model validated by electromyography enabled to evaluate the muscle demand of deep muscles during lifting tasks.

Asymmetries in joint work during multi-joint movement after anterior cruciate ligament reconstruction: a pilot study.

After anterior cruciate ligament reconstruction (ACL-R), many studies have reported a deficit of performance on the injured leg during multi-joint tasks. However, the total mechanical joint work (WTotal ), parameter best related to the vertical displacement of the body mass center during vertical jumping, has not yet been studied. The aim of this research was to compare asymmetries between ACL-R subjects and healthy matched subjects, through the analysis of the kinematics and kinetics during a single-leg squat jump. Asymmetries are defined by the Limb Symmetry Index (LSI). A greater LSI was observed for WTotal in the ACL-R group than in the healthy group. There was no difference in LSI for knee joint work between the two groups, while the LSI for hip and ankle joint work was significantly larger in the ACL-R group. This was explained by greater LSI for the hip and ankle joint range of motion in the ACL-R group than in the healthy group. After ACL-R, patients exhibited greater asymmetries than healthy subjects during single-leg squat jump. Physiotherapists should focus on quality execution of multi-joint movement, especially on hip and ankle joints range of motion in order to reduce asymmetries and to improve vertical jumping performance.

The effects of seat height and foot placement on lumbar spine load during sit-to-stand tasks.

The purpose of this study was to determine the influence of seat height and foot position in the sagittal plane on L5-S1 joint load. Fourteen healthy male adults stood up from a chair with three different seat heights and positions of the feet in the sagittal plane. L5-S1 net joint torque, mechanical work, range of motion and electromyographic activity of the erector spinae muscle were measured. L5-S1 net joint work increased by about 50% from high to low seat position. The mean and peak L5-S1 net joint torques increased about 30% from foot-back to foot-neutral position. These results were reinforced by a greater integrated electromyography signal from the erector spinae from high to low seat position and from foot-back to foot-neutral position. A high chair and placement of the feet behind the knees may be advisable to lessen lumbar load during sit-to-stand movements.

The open Latarjet procedure does not affect scapulohumeral rhythm three months postoperatively.

BACKGROUND: Although it is considered a sensitive indicator of shoulder disabilities, the scapulohumeral rhythm has not been investigated after anterior glenohumeral stabilization with open Latarjet procedure. This study aimed to assess the short-term influence of glenohumeral stabilization on scapulohumeral rhythm in patients who underwent open Latarjet procedure compared to asymptomatic individuals. METHODS: A group of male patients who underwent anterior glenohumeral stabilization by open Latarjet procedure and a healthy group were enrolled in this study. An electromagnetic device was used to record scapulothoracic and glenohumeral joint kinematics during dynamic arm elevation and depression in the scapular plane before and three months after surgery. Then, the three-dimensional dynamic scapulohumeral rhythm was computed bilaterally. SPM ANOVAs were used for statistical analysis. FINDINGS: Twenty-two participants per group were included. The scapulohumeral rhythm of the two groups increased from 1.88 [mean] ± 0.79 [standard deviation] to 2.83 ± 1.52 during the ascending phase, while a decrease followed by a slight increase in the scapulohumeral rhythm were observed during the descending phase. The scapulohumeral rhythm did not significantly differ between the operated and non-operated sides or before and after surgery. When pooling the sides for each group, no significant differences were observed between the groups. INTERPRETATION: The scapulohumeral rhythm in patients who underwent the Latarjet procedure is not modified three months after surgery and is similar to the scapulohumeral rhythm of asymptomatic individuals. These findings suggest that in addition to restoring glenohumeral stability, the open Latarjet procedure preserves shoulder joint coordination three months postoperatively.

Is an ellipsoid surface suitable to model the scapulothoracic sliding plane?

Closed loop kinematic chain approaches are commonly used to assess scapular kinematics but with heterogeneous ellipsoid calibration procedures. This study aimed to assess whether an ellipsoid surface can model the scapulothoracic sliding plane and determine the optimal number of static poses to calibrate the ellipsoid parameters. An intracortical pin with a rigid cluster of four reflective markers was inserted into the left scapular spine of two healthy males (P1 and P2). They performed arm elevations, internal rotations, ball throwing, hockey shooting, and eating movements. Ellipsoid radii and center location were functionally calibrated for each participant and each movement, either based on all frames of a movement or based on a reduced number of frames (from 3 to 200 equally position-distributed frames). Across both participants and all movements, ellipsoid radii varied up to 10.2 cm, 3.9 cm, and 18.4 cm in the antero-posterior, medio-lateral, and cranio-caudal directions, respectively. When all frames of a movement were considered for calibration, the median scapula-to-ellipsoid distance was, on average, 0.52 mm and 0.38 mm for P1 and P2, respectively. When only five frames were considered for ellipsoid calibration, the scapula-to-ellipsoid median distance slightly increased with 0.57 mm and 0.47 mm for P1 and P2, respectively. To conclude, this study highlights that an ellipsoid surface may effectively be appropriate to model the scapulothoracic sliding plane, especially when the calibration is functional, participant- and movement-specific. Furthermore, the number of poses required for the ellipsoid calibration can be reduced to five, minimizing the experimental cost.

Normative values for internal and external glenohumeral rotation strength in rugby players: A systematic review with meta-analysis.

This systematic review aims to provide normative values for internal and external glenohumeral rotation strength in rugby players. From the inception to March 2021, the search strategy was (strength OR torque) AND shoulder AND rugby using PubMed, Scopus, Web of Science, and SPORTDiscus databases, with no language restrictions. This systematic review includes 15 articles involving 573 rugby players and presenting internal or external glenohumeral rotation strength values. Two main methods are used to assess glenohumeral rotation strength in rugby players: isokinetic and isometric methods; in the isometric method, the upper arm is abducted at either 0° or 90°. Owing to differences in isokinetic procedures and a lack of studies assessing isometric strength when the upper arm is in a neutral position, normative internal or external glenohumeral rotation strength values are only provided for isometric contractions when the upper arm is abducted at 90° based on 311 shoulders of 163 male rugby union players, with 2.04 ± 0.15 N.kg-1 and 2.11 ± 0.13 N.kg-1 for internal and external glenohumeral rotation strength, respectively. These findings may help strength and conditioning coaches and physical therapists, provide objective evidence when deciding whether or not rugby union players should return to sport.

Effect of arm swing on effective energy during vertical jumping: experimental and simulation study.

Arm swing helps to increase vertical jump height (VJH), in part by a greater hip joint muscle work. The force-velocity relationship has been put forward to explain the increase in hip joint work. Nevertheless, the efficacy ratio, muscle shortening length, and active state might be parameters that affect the effective energy and then VJH. The purpose of this study was to evaluate the influence of arm swing on effective energy in vertical jumping. Eight subjects performed maximal squat jumps with (SJ arm ) and without arm swing (SJ). A simulation model of the musculo-skeletal system was applied. For subjects and simulation, VJH was about 20% higher during SJ arm. In subjects, this was explained by the shoulder joint work (34%) and an increase of L5-S1 joint work (66%). In simulated jump, effective energy increase during SJ arm (+80.74 J) was related to an improvement of the total muscle work and not to the efficacy ratio. The increase in total muscle work was due to anterior deltoid work and to greater erector spinae, biceps femoris, and gluteus work. The greater muscle works were explained by a slower shortening velocity for all the muscles and by a greater shortening length and active state for the biceps femoris.

Influence of marker weights optimization on scapular kinematics estimated with a multibody kinematic optimization.

Scapular kinematic estimates are altered by soft tissue artefacts, therefore experimental and numerical methods should be developed to improve their accuracy. This study aimed to assess the influence of weights applied to the scapula markers within a closed-loop multibody kinematic optimization on scapular kinematic estimates. Fifteen healthy volunteers performed static postures mimicking analytical, daily living and sport movements. Scapulo-thoracic angles were computed either from a scapula locator as the reference, or from a closed-loop multibody-kinematic optimization (MKO) including a participant-specific point-on-ellipsoid scapulothoracic joint. Weights applied to scapula markers in the MKO were optimized to minimize the difference in scapular orientation from the reference. Optimizing weighting sets significantly (p < 0.0001) improved scapular orientation from 0.9° to 12.1° in comparison to scapular kinematics estimated with non-optimized weighting sets. The mean optimized weighting set contained no neglectable weight for all markers from the acromion to the medial border of the scapular spine but showed no significant difference (p = 0.547) compared to homogeneous weights. Optimized weighting sets were participant- and movement- specific. To conclude, homogenous weights applied on redundant markers located from acromion to scapular medial border spine are recommended when estimating scapular kinematics in upper limb MKO.

Uncertainty analysis and sensitivity of scapulothoracic joint angles to kinematic model parameters.

The purposes of this study were to determine the influence of kinematic model parameter variability on scapulothoracic angle estimates, and to define which parameters of the kinematic model have the largest effect on scapulothoracic angle estimates. Nominal subject-specific kinematic models of nine participants were implemented. Fifteen parameters of the nominal models relative to the clavicle length, ellipsoid, sternoclavicular and acromioclavicular joint centers, and contact point location were altered from - 1 to 1 cm. Then, scapulothoracic angles were computed during four movements using multibody kinematic optimizations for nominal and altered models. The percentage of scapulothoracic angle variance explained by each parameter of the kinematic model was computed using Effective Algorithm for Computing Global Sensitivity Indices. When altering simultaneously the 15 parameters of the kinematic model, scapulothoracic angles varied up to 50°. For all movements and degrees of freedom, the clavicle length significantly explained the largest part of scapulothoracic angle variance (up to 25%, p < 0.01). In conclusion, kinematic model parameters need to be estimated accurately to avoid any bias in scapulothoracic angle estimates especially in a clinical context. The present sensitivity analysis may also be used as a benchmark for future works focusing on improving shoulder kinematic models. The curves represent mean scapulothoracic angles computed with the nominal model and their variability when kinematic model parameters are altered. The colormap graphs represent the percentage of scapulothoracic angle variance explained by each parameter of the kinematic model.

Designing 2-aminoimidazole alkaloids analogs with anti-biofilm activities: structure-activities relationships of polysubstituted triazoles.

In order to discover novel probes that may help in the investigation and the control of bacterial biofilms, we have designed a library of triazole-based analogs of 2-aminoimidazole marine alkaloids: naamine A and isonaamine A. Twenty-two compounds were screened for their biofilm inhibitory activity against two strains of Gram-negative bacteria. Four compounds were shown to act as non-toxic inhibitors of biofilm development without effect on bacterial growth even at high concentrations (100 µM).

Closed-loop multibody kinematic optimization coupled with double calibration improves scapular kinematic estimates in asymptomatic population.

Non-invasive methods still need to better estimate scapular kinematics because of soft tissue artifact issue. This study aimed to develop and assess new procedures to estimate scapular kinematics by combining closed kinematic chain optimization and double calibration. Sixteen healthy volunteers performed static postures mimicking analytical and daily living movements. Scapulo-thoracic angles were computed either with a scapula locator (Ref), or with a closed-loop multibody kinematic optimization (Ell) or with double calibration involving linear (DClin), exponential (DCexp) or logarithmic (DClog) correction. Double calibration corrections enforced scapulo-thoracic angles to be the same than those measured with Ref at the end of the movement performed. DClin and DClog significantly (p < 0.01) reduced scapulo-thoracic misorientation for at least the second third of the movement with averaged improvement ranging from 9° to 32°. Moreover, for arm elevation in the sagittal plane, internal rotations and mimicking hair combing, the beneficial effect of DClin and DClog propagates up to half of the movement. To conclude, when a kinematic chain is required, coupling double calibration (using either linear or logarithmic correction), to a closed-loop multibody kinematic optimization is an efficient and fast method in regard with improvement in scapular kinematic estimates in healthy population.

Effect of rocker shoes and running speed on lower limb mechanics and soft tissue vibrations.

Previous studies have shown a possible effect of running speed and the sole material of footwear on lower-limb mechanics and soft tissue vibrations, while little information has been offered concerning the influence of the shape of the footwear's sole. The purpose of this study is to assess the effect of running speed and rocker shoes on muscular activity, ground reaction force, and soft tissue vibrations. Twenty participants performed heel-toe running with two shoes, differentiated only by their sole shape (i.e. rocker and non-rocker), at four running speeds. Ground reaction force and electromyograms of the gastrocnemius medialis and vastus lateralis were measured, and soft tissue accelerations of the same muscles were recorded with tri-axial accelerometers. A continuous wavelet transform was applied to the accelerometer's signals to analyse them in the time-frequency domain. The rocker of the shoes did not change the muscular activations, ground reaction force, nor power of soft tissue vibrations. In opposite, increased running speed led to an augmentation of all of the measured parameters. Interestingly, running speed augmentation led to a greater increase in high frequencies component of soft tissue vibrations (25-50 Hz, 242%) than lower ones (8-25 Hz, 111%). Consequently, we indicated a 10% increase in the relative part of the high frequencies of the total power. In conclusion, although rocker shoes have shown an effect on lower-limb kinetics in some studies, no influence on soft tissue vibration is denoted. By contrast, soft tissue vibrations may be modulated by changing running speed.

Sensitivity of Shoulder Musculoskeletal Model Predictions to Muscle-Tendon Properties.

OBJECTIVE: While the sensitivity of estimated muscle forces to muscle-tendon properties is well documented for the lower limbs, little is known about the shoulder and upper limbs. The purpose of this study was to assess the sensitivity of estimated shoulder muscle forces and scapulohumeral joint force to muscle-tendon properties. METHODS: One healthy male participant performed arm flexions and simulated throwing maneuvers. Kinematics were recorded using intra-cortical pins. Muscle forces were estimated using static optimization with the generic delft shoulder and elbow in OpenSim, and scapulohumeral joint forces were calculated from the estimated forces. Then, variations from -25% to +25% of the nominal values of the tendon slack length, the optimal fiber length, the maximal isometric force, and the pennation angle were applied to the musculoskeletal model to compute affected muscle forces and scapulohumeral joint force. RESULTS: The variations in muscle-tendon properties led to changes up to 9.6 N or 174% in the muscle nominal forces. The more sensitive muscles were those that produced the greatest force: the rotator cuff muscles and the prime movers specific to the task. Among the four muscle-tendon properties, the maximal isometric force and the optimal fiber length had the greatest influence on the muscle force variability. Glenohumeral force was slightly influenced by muscle-tendon properties (<8%). CONCLUSION: Generic models (i.e., those without personalization of muscle-tendon properties) can lead to misinterpretations of muscle force. Efforts should focus on the maximal isometric force and the optimal fiber length of the rotator cuff muscles and prime movers.

Technical considerations in lateral extra-articular reconstruction coupled with anterior cruciate ligament reconstruction: A simulation study evaluating the influence of surgical parameters on control of knee stability.

BACKGROUND: Surgical parameters such as the selection of tibial and femoral attachment site, graft tension, and knee flexion angle at the time of fixation may influence the control of knee stability after lateral extra-articular reconstruction. This study aimed to determine how sensitive is the control of knee rotation and translation, during simulated pivot-shift scenarios, to these four surgery settings. METHODS: A computer model was used to simulate 625 lateral extra-articular reconstructions based upon five different variations of each of the following parameters: femoral and tibial attachment sites, knee flexion angle and graft tension at the time of fixation. For each simulated surgery, the lateral extra-articular reconstruction external rotation moment at the knee joint center was computed during simulated pivot-shift scenarios. The sensitivity of the control of knee rotation and translation to a given surgery setting was assessed by calculating the coefficient of variation of the lateral extra-articular reconstruction external rotation moment. FINDINGS: Graft tension had minimal influence on the control of knee rotation and translation with less than 2.4% of variation across the scenarios tested. Control of knee rotation and translation was the least affected by the femoral attachment site if the knee was close to full extension at the time of graft fixation. The choice of the tibial attachment site was crucial when the femoral fixation was proximal and posterior to the femoral epicondyle since 15 to 67% of variation was observed in the control of knee rotation and translation. INTERPRETATION: Femoral and tibial attachment sites as well as knee flexion angle at the time of fixation should be considered by surgeons when performing lateral extra-articular reconstruction. Variation in graft tension between the ranges 20-40 N has minimal influence on the control of knee rotation and translation.

Scapular kinematics during scaption in competitive swimmers.

This study aimed (1) to describe and compare scapular kinematics between three groups of swimmers of different levels and a group of non-swimmers, and (2) to assess whether swimming practice alters the asymmetries in scapular kinematics between the dominant and non-dominant sides, both during unilateral arm raising and lowering in the scapular plane. Scapular kinematics were assessed bilaterally during arm raising and lowering in the scapular plane using an electromagnetic system in 42 healthy males, which were split into four groups: control (n = 11), adolescent elite swimmers (n = 11), adult elite swimmers (n = 10), and club-level adult swimmers (n = 10). One-Way ANOVA SPM(t) on two repeated measures showed that the three groups of swimmers had more protracted shoulder between 30° and 90° of arm raising and lowering (p < .001). The three groups of swimmers presented no bilateral difference in scapular upward rotation, while the dominant scapula was more upwardly rotated than the non-dominant one between 74° and 104° of arm elevation in the control group (p < .001). The scapula of adult elite swimmers was more internally rotated between 67° and 116° of humeral elevation during arm raising, and between 81° and 54° during arm lowering in comparison to the other swimming and control groups (p ≤ .02), who presented similar scapular positioning in internal rotation. In conclusion, the findings of the study pointed out that swimming practice generated protracted shoulders and removed bilateral differences in scapular upward rotation during scaption, while accumulation of swimming practice at elite level enlarged scapular internal rotation.

Main component of soft tissue artifact of the upper-limbs with respect to different functional, daily life and sports movements.

Soft tissue artifact (STA) is the main source of error in kinematic estimation of human movements based on skin markers. Our objective was to determine the components of marker displacements that best describe STA of the shoulder and arm (i.e. clavicle, scapula and humerus). Four participants performed arm flexion and rotation, a daily-life and a sports movement. Three pins with reflective markers were inserted into the clavicle, scapula and humerus. In addition, up to seven skin markers were stuck on each segment. STA was described with a modal approach: individual marker displacements or marker-cluster (i.e. translations, rotations, homotheties and stretches) relative to the local segment coordinate system defined by markers secured to the pins. The modes were then ranked according to the percentage of total STA energy that they explained. Both individual skin marker displacements and marker-cluster geometrical transformations were task-, location-, segment- and subject-specific. However, 85% of the total STA energy was systematically explained by the rigid transformations (i.e. translations and rotations of the marker-cluster). In conclusion, large joint dislocations and limited efficiency of least squares bone pose estimators are expected for the computation of upper limb joint kinematics from skin markers. Future developments shall consider the rigid transformations of marker-clusters in the implementation of an STA model to reduce its effects on kinematics estimation.

Analysis of the tennis racket vibrations during forehand drives: Selection of the mother wavelet.

The time-frequency analysis of the tennis racket and hand vibrations is of great interest for discomfort and pathology prevention. This study aimed to (i) to assess the stationarity of the vibratory signal of the racket and hand and (ii) to identify the best mother wavelet to perform future time-frequency analysis, (iii) to determine if the stroke spin, racket characteristics and impact zone can influence the selection of the best mother wavelet. A total of 2364 topspin and flat forehand drives were performed by fourteen male competitive tennis players with six different rackets. One tri-axial and one mono-axial accelerometer were taped on the racket throat and dominant hand respectively. The signal stationarity was tested through the wavelet spectrum test. Eighty-nine mother wavelet were tested to select the best mother wavelet based on continuous and discrete transforms. On average only 25±17%, 2±5%, 5±7% and 27±27% of the signal tested respected the hypothesis of stationarity for the three axes of the racket and the hand respectively. Regarding the two methods for the detection of the best mother wavelet, the Daubechy 45 wavelet presented the highest average ranking. No effect of the stroke spin, racket characteristics and impact zone was observed for the selection of the best mother wavelet. It was concluded that alternative approach to Fast Fourier Transform should be used to interpret tennis vibration signals. In the case where wavelet transform is chosen, the Daubechy 45 mother wavelet appeared to be the most suitable.