Litomosa chiropterorum Ortlepp, 1932 (Nematoda: Filarioidea) from a South African miniopterid: redescription, Wolbachia screening and phylogenetic relationships with Litomosoides.

69 Miniopterus notalensis, type host of the onchocercid Litomosa chiropterorum, were collected in caves in the Western Province and Gauteng Province, South Africa. The prevalence of these filariae was about 50 %. The microfilaria is folded, as in other Litomosa and an area rugosa composed of cuticular bosses is present in the male posterior region. L. chiropterorum is close to the species parasitic in other Miniopterus spp. and some Rhinolophus spp. from Africa, Madagascar and Europe; it is unique with the expanded anterior extremity and the four cephalic submedian bosses. The molecular analysis of L. chiropterorum, the first done with Litomosa species from a bat, supports the hypothesis that Litomosa and Litomosoides, which have an exceptionally large buccal capsule in common, form a group in which Litomosa has a basal position. Interestingly, L. chiropterorum does not harbour Wolbachia, as proved with immunohistological staining and PCR screening using the 16S rDNA gene as target. This is contrary to L. westi from rodents and the majority of the Litomosoides species parasitic in bats or rodents. The absence of Wolbachia in a filarioid group considered ancient based on traditional and molecular approaches opens interesting scenarios on the evolution of the endosymbionts spread through filarial lineages.

Post mortem identification of Kalicephalus colubri colubri (Nematoda: Diaphanocephalidae) in a captive mole snake (Pseudaspis cana) in South Africa.

Necropsy examination of a captive emaciated, dehydrated adult female Mole snake (Pseudaspis cana) in October 2007 revealed multiple cutaneous abscesses. Other findings included renal and hepatic atrophy, hepatic haemosiderosis, multifocal granulomatous hepatitis associated with acid-fast bacteria as well as pulmonary congestion and oedema. Large numbers of the nematode Kalicephalus colubri colubri were recovered from the oesophagus and stomach, representing the 1st reported case of K. colubri from a Mole snake in South Africa. The lesions caused by K. c. colubri were insignificant, but the presence of worms may have contributed to weight loss.

The helminth community of Helmeted Guineafowls, Numida meleagris (Linnaeus, 1758), in the north of Limpopo Province, South Africa.

The helminths of 15 Helmeted Guineafowls were collected in the north of Limpopo Province, South Africa. A total of 11 cestode, ten nematode and a single acanthocephalan species were present. Species richness ranged from 8 to 16 species per host, and nine core and nine secondary species accounted for 40.9% of the component parasite community. The remaining 18.2% comprised satellite species. Core species represented 91% of all the worms present. Individual intensities ranged from 66 to 2,724 per host and overdispersion was pronounced. There were no significant differences regarding the abundance and species richness between male and female hosts. The number of component species and overall abundance did not differ significantly between juvenile and adult hosts, but Cyrnea parroti was significantly more abundant, and the prevalence of Hadjelia truncata was higher in young birds than in adults. In contrast, Gongylonema congolense and Porogynia paronai were absent in juveniles, but had a prevalence of 60% and 70%, respectively, in adults. Pairwise Spearman's rank correlation yielded one positive and 10 negative significant species correlations. A single trematode, Dicrocoelium macrostomum, was collected from five of nine guineafowls, but was not included in the helminth community study.

Helminth parasites of Natal long-fingered bats, Miniopterus natalensis (Chiroptera: Miniopteridae), South Africa.

The helminth community infecting Miniopterus natalensis was studied at two localities, the De Hoop Nature Reserve (DHNR) (n = 57), Western Cape Province and Pretoria (n = 12), Gauteng Province, South Africa. Hosts from the DHNR had formed part of an earlier, unrelated study and were all pregnant females. A single hymenolepidid cestode species, the nematodes Molinostrongylus ornatus and Litomosa chiropterorum together with nematodes of the subfamily Capillariinae were present at both study sites, while a single digenean, Allassogonoporus sp., was only found in hosts from the DHNR. The prevalence of helminth infections was high at both localities, 68.4% in the DHNR and 77.7% in Pretoria, whereas the mean intensity of infection was low at the DHNR (3.76 +/- 3.15), but higher in Pretoria (10.4 +/- 9.9). Molinostrongylus ornatus and, to a lesser extent L. chiropterorum, were the main contributors to the higher intensities in Pretoria. The species richness ranged from 0 to 4 at both localities.

Nematodes from Swainson's spurfowl Pternistis swainsonii and an Orange River francolin Scleroptila levaillantoides in Free State Province, South Africa, with a description of Tetrameres swainsonii n. sp. (Nematoda: Tetrameridae).

Five Swainson's spurfowl collected in Free State Province, South Africa, were examined for helminth parasites, and the nematodes Acuaria gruveli, Cyrnea parroti, Gongylonema congolense, Subulura dentigera, Subulura suctoria and a new Tetrameres species were recovered. Their respective prevalence was 100, 20, 80, 20, 20 and 20%. These nematodes are all new parasite records for Swainson's spurfowl, and Acuaria gruveli constitutes a new geographical record as well. A single specimen of Cyrnea eurycerca was found in an Orange River francolin, representing a new host and geographical record for this parasite. The new species, for which the name Tetrameres swainsonii is proposed, can be differentiated from its congeners by a combination of the following characters of males: two rows of body spines, a single spicule which is 1152-1392 microm long, and eight pairs of caudal spines arranged in two ventral and two lateral rows of four spines each. The single female has the globular shape typical of the genus.

Tetrameres numida n. sp. (Nematoda: Tetrameridae) from Helmeted guineafowls, Numida meleagris (Linnaeus, 1758), in South Africa.

Tetrameres numida n. sp. from the proventriculus of Helmeted guineafowls, Numida meleagris, in South Africa is described from eight male and four female specimens. The new species shares some characteristics with other Tetrameres species, but can be differentiated by a unique combination of characters. It bears two rows of cuticular spines extending over the whole length of the body and possesses two spicules. The left spicule measures 1699-2304 microm and the right one 106-170 microm. Caudal spines are arranged in three ventral and three lateral pairs and the tail is 257-297 microm long. Diagnostic criteria of some of the previously described species of the genus Tetrameres from Africa and other parts of the world have been compiled from the literature and are included here.

Helminths of guineafowls in Limpopo Province, South Africa.

Between July 2005 and November 2006 the gastro-intestinal helminths of 15 Helmeted guineafowls and a single Crested guineafowl from Musina, Limpopo Province were examined, and in July and August 2005 helminths were collected from five Helmeted guineafowls from Mokopane in the same province. The acanthocephalan Mediorhynchus gallinarum, the cestodes Abuladzugnia gutterae, Davainea nana, Hymenolepis cantaniana, Numidella numida, Octopetalum numida, Ortleppolepis multiuncinata, Porogynia paronai, Raillietina angusta, Raillietina pintneri, Raillietina steinhardti and Raillietina sp. and the nematodes Ascaridia numidae, Cyrnea parroti, Gongylonema congolense, Hadjelia truncata, Sicarius caudatus, Subulura dentigera, Subulura suctoria, Subulura sp., Tetrameres numida and an unidentified subulurid were recovered. A single trematode species, Dicrocoelium macrostomum, was present in the liver. Mediorhynchus gallinarum, A. gutterae, O. multiuncinata, H. truncata and S. caudatus are recorded for the first time from Helmeted guineafowls, as well as from South Africa. South Africa is a new geographic record for D. macrostomum, G. congolense and D. nana. Subulura suctoria, G. congolense and H. truncata from the Crested guineafowl constitute new host-parasite associations.

A check list of the helminths of guineafowls (Numididae) and a host list of these parasites.

Published and personal records have been compiled into a reference list of the helminth parasites of guineafowls. Where data on other avian hosts was available these have been included for completeness' sake and to give an indication of host range. The parasite list for the Helmeted guineafowls, Numida meleagris, includes five species of acanthocephalans, all belonging to a single genus, three trematodes belonging to three different genera, 34 cestodes representing 15 genera, and 35 nematodes belonging to 17 genera. The list for the Crested guineafowls, Guttera edouardi, contains a single acanthocephalan together with 10 cestode species belonging to seven genera, and three nematode species belonging to three different genera. Records for two cestode species from genera and two nematode species belonging to a single genus have been found for the guineafowl genus Acryllium. Of the 70 helminths listed for N. meleagris, 29 have been recorded from domestic chickens.

Chemokine scavenging by the human cytomegalovirus chemokine decoy receptor US28 does not inhibit monocyte adherence to activated endothelium.

The human cytomegalovirus has found smart ways to exploit the chemokine network in order to subvert immune attack. Chemokines trigger the arrest and firm adhesion of inflammatory cells to the vascular wall. Scavenging of chemokines by viral decoy receptors, such as US28, might prevent arrest of leukocytes to the vascular wall and impair an antiviral immune response. We determined the effect of chemokine scavenging by endothelium-expressed signaling mute US28 (US28R129A) on static monocyte adhesion. Despite the chemokine scavenging capacity of US28R129A, expression of this construct by endothelial cells was insufficient to disrupt leukocyte adhesion to cytokine-activated monolayers. Our results suggest that the concentrations of chemokines that trigger firm leukocyte adhesion are too high to be efficiently scavenged by viral chemokine decoy receptors like US28. From the results of this experimental model a role for US28 in viral immune evasion by chemokine scavenging would appear therefore unlikely.

An examination of the infracommunities and component communities from impala (Aepyceros melampus) in the Kruger National Park, South Africa.

The intestinal helminth parasites of the impala from the Kruger National Park, South Africa, were examined to describe the parasite community structure. Demographic variation and the associated differences in behavior were used to further investigate the patterns of community composition. Monte Carlo simulations were performed to test for differences in species richness and mean abundance between the various demographic groups, and nonmetric multidimensional scaling ordination was used to compare community composition. Seventeen species of nematodes, totaling more than 1.3 million worms, were recovered. Males harbored a greater number of nematode species than did females, but adult females were more heavily infected than their male counterparts. Lambs acquired infections early in life, and their parasite community composition rapidly approached that of the older animals. The parasite community in the juvenile and adult males was significantly different from the community of the adult females. These data suggest that social and feeding behavior of the different age-sex classes structure the parasite component community of impala. Additionally, the distinction between common and rare parasites, and their classification in other herbivores, implies complex transmission dynamics that includes extensive species sharing within the Kruger National Park.

Mediorhynchus gallinarum (Acanthocephala: Gigantorhynchidae) in Helmeted guineafowls, Numida meleagris, in the Kruger National Park, South Africa.

Mediorhynchus gallinarum was recovered from the small intestines of 36 of 50 Helmeted guineafowls sampled from August 1988 to May 1989. The intensity of infection ranged from 1-141 worms per host, with a mean intensity of 23.2 (+/- 34) and a median intensity of 5. The Wilcoxon-Mann-Whitney test revealed no significant differences between the mean worm burdens of male and female birds at the 5% level (P > 0.05). Slightly more female than male acanthocephalans were collected. The majority (63.4%) of females had eggs with fully-developed embryos, 9% had immature eggs, 21.2% had no eggs and the egg status of 6.4% could not be determined. No seasonal pattern of intensity of infection emerged from the data, but worm burdens were markedly higher after good rains in February 1989. South Africa constitutes a new geographic record for M. gallinarum.

Check-list of the pentastomid parasites crocodilians and freshwater chelonians.

Based on published records and own data a summary is given of the geographical distribution of the currently known species of pentastomid parasites infecting crocodiles and alligators, as well as freshwater chelonians. A brief generic diagnosis is provided for each genus. Fourteen out of the currently 23 living crocodilian species have been recorded as being host to one or more pentastomes. Out of the 32 pentastome species six are considered species inquirendae. Presently, six genera of crocodilian pentastomes, Agema, Alofia, Leiperia, Sebekia, Selfia and Subtriquetra are recognized. African crocodiles harbour eight pentastome species, six of which have been recorded from the Nile crocodile, Crocodylus niloticus. Three species belong to the genus Sebekia, Alofia being represented by two and Leiperia by only one species. Two species, Alofia parva and Agema silvae-palustris, occur in the dwarf crocodile, Osteolaemus tetraspis, and the slender-snouted crocodile, Crocodylus cataphractus, exclusively, but a single Sebekia species is shared with the Nile crocodile. The genus Agema is endemic to the African region. Infective stages of the pentastome Subtriquetra rileyi, thought to utilize Nile crocodiles as final hosts, have been recovered only from fishes. The largest number of pentastome species is found in the Australasian region. Of these, the Indo-Pacific crocodile, Crocodylus porosus, harbours seven, representing the genera Alofia, Sebekia, Leiperia and Selfia. Selfia is exclusive to the latter host. The genus Subtriquetra has been reported from "Indian crocodiles", a term possibly referring to either Crocodylus palustris, Crocodylus porosus or Gavialis gangeticus. Ten species of pentastomes parasitizing the crocodilian genera Alligator, Caiman, Crocodylus and Melanosuchus have been recorded from the Neotropical region including the southern states of the North American continent. The two most wide-spread pentastome genera, Alofia and Sebekia, have been recorded together with representatives of the genus Subtriquetra and immature and larval forms of Leiperia. To date the two monospecific genera, Pelonia, from two terrapin species, Pelusios sinuatus and Pelomedusa subrufa, in South Africa, and Diesingia from Hydraspis geoffroyana and Hydromedusa tectifera in South America, are the only chelonian pentastomes recovered world-wide. A possible exception is the crocodilian pentastome Sebekia mississippiensis which can reach maturity in experimentally infected terrapins.

Helminth parasites of gemsbok (Oryx gazella) in the Klein Karoo.

The number and species of helminth parasites from three gemsbok (Oryx gazella) were recorded, and their faecal nematode egg counts and the level of pasture contamination determined. Six nematode genera were recovered and four species identified, of which Trichostrongylus rugatus was the most prevalent. Other nematode species recovered were Cooperia sp., Agriostomum sp., Haemonchus contortus, Nematodirus spathiger and Ostertagia ostertagi. None of the worms were present in all animals studied, and no new host associations were found. Cysticerci were recovered from the mesenteries of one gemsbok and a further two unidentifiable helminths were recovered from the abomasum and the kidney fat layer of another antelope.

Eustrongylides sp. (Nematoda: Dioctophymatoidea) from the stomach of a Nile crocodile, Crocodylus niloticus Laurenti, 1768, in Botswana.

During a study conducted between 2003 and 2005 on the diet of Nile crocodiles in Botswana, two young adult nematodes, one male and one female, belonging to the genus Eustrongylides Jägerskiöld, 1909 were recovered from the stomach contents of one of these animals. The caudal bursa of the male is present and the ejaculatory duct could be identified, but the spicule could not be seen. The vulva of the female has opened and the anus is situated on a terminal protruberance. Measurements and drawings of these specimens are provided, together with some data on the occurrence and life-cycles of members of the genus Eustrongylides in crocodilians world-wide and in African hosts in particular. Piscivorous birds are the usual final hosts of these nematodes. It is probable that the specimens described herein had developed in a paratenic fish host, and that the latter had been eaten by the crocodile.

First record of Cylicospirura (Cylicospirura) felineus (Chandler, 1925) Sandground, 1933 (Nematoda: Spirocercidae) from a domestic cat in South Africa.

Cylicospirura (Cylicospirura) felineus (Chandler, 1925) Sandground, 1933 is reported from a cat in South Africa for the first time. The nematode was present in a gastric parasitic nodule in a male domestic cat, and three males were recovered as well as the anterior parts of three and the posterior parts of two gravid females. The heads of two specimens of undetermined sex were also found. Part of the removed parasitic nodule was processed for histopathological examination. The parasitic nodule was located in the submucosa and slightly expanded into the muscular layer. In its centre were small necrotic areas containing debris of inflammatory cells, adult nematodes and numerous bacterial colonies. Neutrophils, eosinophils, plasma cells and lymphocytes, as well as fibroblasts, were the predominant cell types. The serosal surface of the stomach remained unaffected. Some comparative morphological as well as ecological data concerning Cylicospirura (Cylicospirura) Vevers, 1922 in other feline hosts, mainly from Australia, India and North America, are included.

Gastric nematodes of nile crocodiles, Crocodylus niloticus Laurenti, 1768, in the Okavango River, Botswana.

The ascaridoid nematodes Dujardinascaris madagascariensis Chabaud & Caballero, 1966, Dujardinascaris dujardini (Travassos, 1920), Gedoelstascaris vandenbrandeni (Baylis, 1929) Sprent, 1978 and Multicaecum agile (Wedl, 1861) Baylis, 1923 were recovered from the stomach contents of Crocodylus niloticus Laurenti, 1768 from the Okavango River, Botswana, together with Eustrongylides sp., a dioctophymatoid nematode usually parasitizing piscivorous birds. Dujardinascaris madagascariensis was present in most of the infected hosts, while the remaining species were mostly represented in single collections in one to three hosts. All four ascaridoid nematodes represent new geographic records.

Anguillicola papernai (Nematoda: Anguillicolidae) and other helminths parasitizing the African longfin eel Anguilla mossambica.

The swim bladder nematode Anguillicola papernai Moravec & Taraschewski, 1988 has been investigated as regards its occurrence in longfin eels Anguilla mossambica (Peters) in rivers in South Africa. A. papernai revealed a prevalence of around 50% and a mean intensity of about 6 adult worms at 1 sampling site but were less abundant in 3 others. Field observations suggest a more narrow habitat preference than that of Anguillicola crassus and a seasonal pattern of abundance. African longfin eels harboured a poor helminth community. In addition to A. papernai, 2 gastro-intestinal nematodes occurred, the stomach worm Heliconema longissimum Ortlepp, 1923 as the dominant species, and the intestinal Paraquimperia africana Moravec, Boomker & Taraschewski, 2000. Experiments were undertaken using European eels Anguilla anguilla (Linnaeus) and copepods as laboratory hosts. The morphology of larvae and adult parasites obtained from these experimental hosts is described. The ultrastructure of adult worms recovered from wild longfin eels was studied. The 'papilla-like excrescences of fibrous structure' on the adult worms' cuticle, as mentioned in the original description, are in fact the attachment points of thick cords of fibers interconnecting the epicuticle with the hypodermis. Such a structure has not yet been described from any other species of Anguillicola Yamaguti, 1935. At present in South Africa, Mozambique and Madagascar attempts are on the way to establish an eel management like in Asia and Europe including eel farming. In this context, care should be taken to prevent the introduction of non-endemic eel parasites into Africa and Madagascar. On the other hand, the future commercial management of African eel species should not lead to the spread of A. papernai or other parasites of African eel species to Europe or elsewhere. In this study A. papernai has been experimentally demonstrated to be capable of reproducing in the European eel and of using European copepods as intermediate hosts.

Helminths in sympatric populations of mountain reedbuck (Redunca fulvorufula) and gray rhebok (Pelea capreolus) in South Africa.

Helminths of mountain reedbuck (Redunca fulvorufula fulvorufula) and gray rhebok (Pelea capreolus) were investigated in South Africa between June 1999 and February 2002. Forty-one mountain reedbuck were culled at Sterkfontein Dam Nature Reserve over 8 different periods, and 25 mountain reedbuck were culled at Tussen die Riviere Nature Reserve over 3 different periods. A total of 17 kinds of helminths were found at the 2 sites, including 15 nematodes, 1 trematode, and 1 cestode. At Sterkfontein, the most prevalent and abundant species were Cooperia yoshidai, Longistrongylus schrenki, and Haemonchus contortus, with the latter 2 being more abundant during November/December than at other times of the year, probably because infective larvae increased on pasture at that time. No statistical differences were found in parasite loads between male and female mountain reedbuck. No correlation was found between fecal egg counts and adult worm counts or between parasite counts and body condition. At Tussen die Riviere, helminths in mountain reedbuck were less prevalent and abundant than at Sterkfontein. The most important species were Nematodirus spathiger, Trichostrongylus falculatus, and Cooperia rotundispiculum. Four gray rhebok died of natural causes at Sterkfontein, from which 5 kinds of helminths were recovered, including C. yoshidai and Paracooperioides peleae.

Paraquimperia Africana n. sp (Nematoda : Quimperiidae), a new intestinal parasite of the eel Anguilla mossambica Peters, in South Africa.

A new seuratoid nematode of the family Quimperiidae, Paraquimperia africana n. sp., is described from the small intestine of the longfin eel, Anguilla mossambica Peters, from the Eastern Cape Province, South Africa. The new species is characterized mainly by the presence of a ventral sucker in mature males, short spicules (147-171 microm), the number and arrangement of caudal papillae, the postesophageal position of the excretory pore, and by the slender female tail. In this new species, a variability in the number (3-5 pairs) of subventral preanal papillae was observed. Paraquimperia africana is the first representative of the genus in Africa. In view of recent reports, Paraquimperia aditum (Mueller, 1934) is considered a junior synonym of Paraquimperia tenerrima (Linstow, 1878). Paraquimperia xenentodonia Gupta and Bakshi, 1984 is considered a species inquirenda.

Paraspirura bettinae n. sp. from a South African skink with comments on spirurid nematodes from saurians and mammals.

The spirurid nematode, Paraspirura bettinae n. sp., described from Mabuya spilogaster (Scincidae) in South Africa (Molopo Reserve), can be distinguished from the single known species of the genus, Paraspirura mabuyae Sandground, 1936 (also reviewed) by the larger size of the eggs, the shape of the tail of the female, and the smaller body size in both sexes. The cephalic anatomy of Paraspirura spp. does not fit the original description but appears similar to that of spirurid parasites of mammals, Spirura spp. and Protospirura spp. Previous descriptions did not mention the rim and bulges in members of Protospirura. Paraspirura appears close to Protospirura, particularly to Protospirura muricola from the Ethiopian region, but it is distinct because of the persistence of the larval caudal tubercles in the adult stage, the simple right spicule without alae, and the more developed rim and bulges. The species parasitic in saurians may have arisen from Protospirura spp. by capture, or the spirurids of mammals may have been derived from species of Paraspirura and quickly split into a Protospirura line and a more specialized Spirura assemblage.