RESUMO
Bagaza virus (BAGV) has not been reported in birds in South Africa since 1978. We used phylogenetic analysis and electron microscopy to identify BAGV as the likely etiology in neurologic disease and death in Himalayan monal pheasants in Pretoria, South Africa. Our results suggest circulation of BAGV in South Africa.
Assuntos
Infecções por Flavivirus/epidemiologia , Infecções por Flavivirus/virologia , Flavivirus , Flavivirus/classificação , Flavivirus/genética , Flavivirus/ultraestrutura , Infecções por Flavivirus/história , História do Século XXI , Humanos , Filogenia , Vigilância em Saúde Pública , África do Sul/epidemiologia , Proteínas não Estruturais Virais/genéticaRESUMO
We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3 noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study.