RESUMO
Objective: To investigate the safety and efficacy of the Weitan Waifu patch on the postsurgical gastroparesis syndrome (PGS) of gastrointestinal cancer. Methods: The multi-center, double-blind, randomized controlled trial was conducted with superiority design. Patients with PGS of gastrointestinal cancer diagnosed in 4 AAA hospitals and the abdominal symptom manifested as cold syndrome by Chinese local syndrome differentiation were recruited. These patients were randomly divided into two groups according to 1â¶1 proportion. Placebo or Weitan Waifu patch was applied in control group or intervention group, respectively, based on the basic treatments, including nutrition support, gastrointestinal decompression, promoting gastric dynamics medicine.Two acupuncture points (Zhongwan and Shenque) were stuck with placebo in control group or patch in treatment group. The intervention course was 14 days or reached the effective standard. Results: From July 15, 2013 to Jun 3, 2015, 128 participants were recruited and 120 eligible cases were included in the full analysis set (FAS), and 60 cases in each group. 88 cases were included in the per-protocol set (PPS), including 45 cases in the treatment group and 43 cases in the control group. In the FAS, the clinical effective rate in the treatment group was 68.3%, significantly superior than 41.7% of the control group (P=0.003). The medium time of effective therapy in the treatment group was 8 days, significantly shorter than 10 days in the control group (P=0.017). In the FAS, 3 adverse events occurred in the treatment group, including mild to moderate decrustation, pruritus and nausea. The incidence rate of adverse events was 5.0% (3/60) and these symptoms were spontaneously remitted after drug withdrawal. No severe adverse events were observed in the control group. There was no significant difference between these two groups (P=0.244). Conclusion: Weitan Waifu patch is a safely and effectively therapeutic method for patients with PGS (cold syndrome) of gastroenterological cancer. Trial registration: International Standard Randomized Controlled Trial Number Register, ISRCTN18291857.
Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Neoplasias Gastrointestinais/cirurgia , Gastroparesia/tratamento farmacológico , Complicações Pós-Operatórias/tratamento farmacológico , Adesivo Transdérmico , Pontos de Acupuntura , Método Duplo-Cego , Humanos , Síndrome , Adesivo Transdérmico/efeitos adversos , Resultado do TratamentoRESUMO
The new allele A*02:01:119 was initially identified in a Chinese individual by sequence-based typing.
Assuntos
Alelos , Povo Asiático/genética , Sequência de Bases , Éxons/genética , Antígenos HLA-A/genética , Humanos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
A novel allele B*13:68 was identified in a Chinese individual by sequence-based typing method.
Assuntos
Alelos , Antígenos HLA-B/genética , Polimorfismo de Nucleotídeo Único , Povo Asiático , Sequência de Bases , Transplante de Medula Óssea , Feminino , Teste de Histocompatibilidade , Humanos , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNA , Doadores de TecidosRESUMO
A novel allele A*11:97 was identified in a Chinese individual by sequence-based typing method.
Assuntos
Alelos , Antígeno HLA-A11/genética , Teste de Histocompatibilidade/métodos , Análise de Sequência de DNA/métodos , Adulto , Povo Asiático/genética , Sequência de Bases , Éxons/genética , Feminino , Humanos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
The novel allele B*46:01:07 was identified in a Chinese individual by sequence-based typing.
Assuntos
Alelos , Povo Asiático/genética , Antígenos HLA-B/genética , Sequência de Bases , China/etnologia , Éxons/genética , Humanos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
The allele was identified in a Chinese individual by sequence-based typing. HLA-B*40:162 differs from B*40: 06:01 by a single nucleotide at position 272 CâT in exon 2.
Assuntos
Alelos , Éxons/genética , Antígenos HLA-B/genética , Polimorfismo de Nucleotídeo Único , Povo Asiático , China , HumanosRESUMO
In this report we describe a novel allele B*44:127, which was identified in a Chinese voluntary bone marrow donor by sequence-based typing. HLA-B*44:127 showed one nucleotide difference from G to A with HLA-B*44:02:01 at nucleotide 320.
Assuntos
Alelos , Antígenos HLA-B/genética , Mutação Puntual , Análise Mutacional de DNA , HumanosRESUMO
One nucleotide replacement at nucleotide 811 of HLA-A*02:01:01:01 results in a new allele, HLA-A*02:692.
Assuntos
Alelos , Antígeno HLA-A2/genética , Teste de Histocompatibilidade , Análise de Sequência de DNA , Feminino , Humanos , MasculinoRESUMO
The novel allele, A*11:264, was identified in a Chinese individual by sequence-based typing.
Assuntos
Povo Asiático , Medula Óssea/fisiologia , Antígeno HLA-A11/genética , Alelos , Transplante de Medula Óssea , China , Teste de Histocompatibilidade , Humanos , Masculino , Polimorfismo Genético , Alinhamento de Sequência , Análise de Sequência de DNA , Doadores de Tecidos , Organização Mundial da SaúdeRESUMO
A novel allele A*31:126N was identified in a Chinese individual by sequence-based typing method.
Assuntos
Povo Asiático , Medula Óssea/fisiologia , Antígenos HLA-A/genética , Alelos , Transplante de Medula Óssea , China , Feminino , Teste de Histocompatibilidade , Humanos , Polimorfismo Genético , Alinhamento de Sequência , Análise de Sequência de DNA , Doadores de Tecidos , Organização Mundial da SaúdeRESUMO
The cultivated peanut, A. hypogaea L., is an important oil and food crop globally.High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this context, a recombinant inbred line (RIL) of 146 lines was developed by crossing Huayu28 and P76. We developed 433,679 high-quality SLAFs, of which 29,075 were polymorphic. 4,817 SLAFs were encoded and grouped into different segregation patterns. A high-resolution genetic map containing 2,334 markers (68 SSRs and 2,266 SNPs) on 20 linkage groups (LGs) spanning 2586.37 cM was constructed for peanut. The average distance between adjacent markers was 2.25 cM. Based on phenotyping in seven environments, QTLs for oleic acid (C18:1), linoleic acid (C18:2) and the ratio of oleic acid to linoleic acid (O/L) were identified and positioned on linkage groups A03, A04, A09, B09 and B10. Marker2575339 and Marker2379598 in B09 were associated with C18:1, C18:2 and O/L in seven environments, Marker4391589 and Marker4463600 in A09 were associated with C18:1, C18:2 and O/L in six environments. This map exhibits high resolution and accuracy, which will facilitate QTL discovery for essential agronomic traits in peanut.
Assuntos
Arachis/genética , Arachis/metabolismo , Mapeamento Cromossômico/métodos , Ácido Linoleico/metabolismo , Repetições de Microssatélites/genética , Ácido Oleico/metabolismo , Locos de Características Quantitativas/genética , Técnicas de Genotipagem , Fenótipo , Polimorfismo de Nucleotídeo ÚnicoRESUMO
A novel HLA-A*24 allele, A*24:02:61, confirmed in a Chinese individual.
RESUMO
A novel allele A*02:436 was identified in a Chinese individual by sequence-based typing method.
Assuntos
Alelos , Éxons , Antígeno HLA-A2/genética , Mutação Puntual , Doadores de Tecidos , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Códon/química , Feminino , Genótipo , Antígeno HLA-A2/imunologia , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade , Humanos , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
A novel allele DRB1*04:01:17 was identified in a Chinese individual by sequence-based typing method.
Assuntos
Alelos , Éxons , Cadeias HLA-DRB1/genética , Mutação Puntual , Doadores não Relacionados , Sequência de Bases , Expressão Gênica , Genótipo , Cadeias HLA-DRB1/imunologia , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade/métodos , Humanos , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
A novel allele A*24:337 was identified in a Chinese individual by sequence-based typing.
Assuntos
Alelos , Éxons , Antígeno HLA-A24/genética , Mutação Puntual , Doadores não Relacionados , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Clonagem Molecular , Expressão Gênica , Genótipo , Antígeno HLA-A24/imunologia , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade , Humanos , Masculino , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
Malignant cancers commonly invade locally followed by spread through venous or lymphatic channels or both to distant sites. Hemangiogenesis and its relation to tumor growth and metastasis have been extensively studied. However, the role played by lymphangiogenesis in growth and metastasis of cancer has been largely neglected until just recently. Inhibition of lymphangiogenesis, as compared to inhibition of hemangiogenesis, may provide new insights into the mechanisms of cancer metastasis. The current study was designed to examine the in vitro effect of two commonly used inhibitors of hemangiogenesis, angiostatin and thalidomide, on the growth and proliferation of lymphatic endothelial cells isolated from pig thoracic ducts. We first isolated and characterized the lymphatic endothelial (LE) cells using specific markers for VEGFR3 and LYVE-1. The experimental results showed that treatment of the LE cells with these two drugs resulted in a decrease in the rate of cell proliferation in a dose-dependent manner as assessed by MTT assays. Cell migration rate was assessed by the speed of cell migration from the scrape-wound margin, and the results showed that migration of LE cells was also significantly inhibited in a dose-dependent fashion compared to controls. Treatment with angiostatin and thalidomide both resulted in an increase in apoptosis of LE cells as assessed by Hoechst staining and flow cytometry. We conclude that both angiostatin and thalidomide are able to inhibit LE cell growth in a dose-dependent manner and that the inhibition may be through induction of apoptosis.