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1.
Can Vet J ; 54(9): 885-8, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24155496

RESUMO

The clinical and laboratory findings in puppies naturally infected with canine coronavirus (CCoV) and/or canine parvovirus (CPV) were compared with findings in uninfected puppies. Lymphopenia was the only parameter related to CCoV infection that was statistically significant; vomiting, anorexia, lethargy, hemorrhagic fluid diarrhea, leukopenia, lymphopenia, thrombocytopenia, hypoglycemia, and hypoproteinemia were correlated with CPV infection.


Résultats cliniques, hématologiques et biochimiques chez des chiots atteints de l'entérite à coronavirus et à parvovirus. Les résultats cliniques et de laboratoire chez des chiots naturellement infectés par le coronavirus canin (CoVC) et/ou le parvovirus canin (PVC) ont été comparés aux résultats des chiots non infectés. La lymphopénie était le seul paramètre statistiquement significatif associé à l'infection par le CoVC; les vomissements, l'anorexie, la léthargie, la diarrhée liquide hémorragique, la leucopénie, la lymphopénie, la thrombocytopénie, l'hypoglycémie et l'hypoprotéinémie étaient tous associés à l'infection par le PVC.(Traduit par Isabelle Vallières).


Assuntos
Infecções por Coronavirus/veterinária , Coronavirus Canino/isolamento & purificação , Doenças do Cão/virologia , Enterite/veterinária , Infecções por Parvoviridae/veterinária , Parvovirus Canino/isolamento & purificação , Animais , Coinfecção , Infecções por Coronavirus/sangue , Infecções por Coronavirus/patologia , Doenças do Cão/sangue , Doenças do Cão/patologia , Cães , Enterite/sangue , Enterite/patologia , Enterite/virologia , Infecções por Parvoviridae/sangue , Infecções por Parvoviridae/patologia
2.
PLoS Negl Trop Dis ; 13(8): e0007599, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31386662

RESUMO

BACKGROUND: Visceral leishmaniasis (VL) in Brazil is a neglected, vector-borne, tropical parasitic disease that is responsible for several thousand human deaths every year. The transmission route involves sand flies becoming infected after feeding on infected reservoir host, mainly dogs, and then transmitting the Leishmania infantum parasites while feeding on humans. A major component of the VL control effort is the identification and euthanasia of infected dogs to remove them as a source of infection. A rapid, non-invasive, point-of-care device able to differentiate between the odours of infected and uninfected dogs may contribute towards the accurate diagnosis of canine VL. METHODOLOGY/PRINCIPAL FINDINGS: We analysed the headspace volatile chemicals from the hair of two groups of dogs collected in 2017 and 2018 using a bench-top eNose volatile organic chemical analyser. The dogs were categorised as infected or uninfected by PCR analysis of blood samples taken by venepuncture and the number of parasites per ml of blood was calculated for each dog by qPCR analysis. We demonstrated using a robust clustering analysis that the eNose data could be discriminated into infected and uninfected categories with specificity >94% and sensitivity >97%. The eNose device and data analysis were sufficiently sensitive to be able to identify infected dogs even when the Leishmania population in the circulating blood was very low. CONCLUSIONS/SIGNIFICANCE: The study illustrates the potential of the eNose to rapidly and accurately identify dogs infected with Le. infantum. Future improvements to eNose analyser sensor sensitivity, sampling methodology and portability suggest that this approach could significantly improve the diagnosis of VL infected dogs in Brazil with additional potential for effective diagnosis of VL in humans as well as for the diagnosis of other parasitic diseases.


Assuntos
Doenças do Cão/diagnóstico , Leishmaniose Visceral/diagnóstico , Leishmaniose Visceral/veterinária , Óxido Nítrico Sintase Tipo III/análise , Patologia Molecular/métodos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Animais , Animais Domésticos/parasitologia , Brasil/epidemiologia , DNA de Protozoário/análise , Doenças do Cão/sangue , Doenças do Cão/epidemiologia , Cães , Leishmania infantum/genética , Leishmania infantum/isolamento & purificação , Leishmaniose Visceral/epidemiologia , Leishmaniose Visceral/parasitologia , Psychodidae/parasitologia , Sensibilidade e Especificidade
3.
Vet J ; 206(1): 115-7, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26189893

RESUMO

Feline caliciviruses (FCVs) have occasionally been described in cats in association with enteric disease, but an etiological role for these viruses in acute gastroenteritis is still unclear. In this study, molecular characterization of FCV and feline norovirus (FNoV) was undertaken using real-time PCR (RT-PCR) and sequence analysis of the ORF1 region in fecal specimens from 29 diarrheic cats. The specimens were also screened for parvovirus, coronavirus, astrovirus and group A rotavirus. A quantitative one step RT-PCR was also performed to detect and quantitate NoV genogroup IV and the role of these animal caliciviruses in feline gastroenteritis was investigated. This is the first description of enteric FCV and FNoV in South America.


Assuntos
Infecções por Caliciviridae/veterinária , Doenças do Gato/virologia , Surtos de Doenças/veterinária , Gastroenterite/veterinária , Norovirus/isolamento & purificação , Vesivirus/isolamento & purificação , Animais , Brasil , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Doenças do Gato/epidemiologia , Gatos , Gastroenterite/epidemiologia , Gastroenterite/virologia , Norovirus/genética , Filogenia , Vesivirus/genética
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