Thermoregulation During Extended Exercise in the Heat: Comparisons of Fluid Volume and Temperature.

OBJECTIVE: This study aimed to determine the physiological and thermoregulatory responses of individuals exercising in the heat (US military red flag conditions, wet-bulb globe temperature 31.5-32.2ºC) while consuming varied volumes of ambient temperature water and ice slurry. METHODS: Participants (N = 12) walked on a treadmill for 3 hours at approximately 40% peak aerobic capacity in a hot environment while consuming ambient temperature (35.5°C) water (W), ice slurry (0°C, two-thirds shaved ice and one-third water) at a ratio of 2 g·kg(-1) body mass every 10 minutes (FS), and reduced volume ice slurry as described at a rate of 1 g·kg(-1) body mass every 10 minutes (HS). Trials were completed at least 14 days apart, in a randomized, repeated measures design. RESULTS: Percent body weight loss was higher during the HS trial (1.8 ± 0.01%) compared with FS (0.5 ± 0.01%; P < .001) and W (0.6 ± 0.01%; P < .001). Mean rectal temperature at 3 hours was lower during FS (37.8 ± 0.7°C) compared with HS (38.1 ± 0.8°C) and W (38.2 ± 0.8°C) (P = .04 vs HS, and P = .005 vs W, main effect for trial). No differences were found in rectal temperature between HS and W. Heart rate was lower at the end of the third hour during FS (141 ± 10 beats/min) compared with HS (157 ± 19 beats/min) and W (154 ± 18 beats/min) (P = .001 and P = .007, respectively, time × trial interaction). There were no differences in heart rate between HS and W. CONCLUSIONS: The temperature of consumed fluids may be as important as the volume for the management of thermoregulation and other physiological responses for extended work in hot environments.

Postexercise Glycogen Recovery and Exercise Performance is Not Significantly Different Between Fast Food and Sport Supplements.

A variety of dietary choices are marketed to enhance glycogen recovery after physical activity. Past research informs recommendations regarding the timing, dose, and nutrient compositions to facilitate glycogen recovery. This study examined the effects of isoenergetic sport supplements (SS) vs. fast food (FF) on glycogen recovery and exercise performance. Eleven males completed two experimental trials in a randomized, counterbalanced order. Each trial included a 90-min glycogen depletion ride followed by a 4-hr recovery period. Absolute amounts of macronutrients (1.54 ± 0.27 g·kg-1 carbohydrate, 0.24 ± 0.04 g·kg fat-1, and 0.18 ±0.03g·kg protein-1) as either SS or FF were provided at 0 and 2 hr. Muscle biopsies were collected from the vastus lateralis at 0 and 4 hr post exercise. Blood samples were analyzed at 0, 30, 60, 120, 150, 180, and 240 min post exercise for insulin and glucose, with blood lipids analyzed at 0 and 240 min. A 20k time-trial (TT) was completed following the final muscle biopsy. There were no differences in the blood glucose and insulin responses. Similarly, rates of glycogen recovery were not different across the diets (6.9 ± 1.7 and 7.9 ± 2.4 mmol·kg wet weight- 1·hr-1 for SS and FF, respectively). There was also no difference across the diets for TT performance (34.1 ± 1.8 and 34.3 ± 1.7 min for SS and FF, respectively. These data indicate that short-term food options to initiate glycogen resynthesis can include dietary options not typically marketed as sports nutrition products such as fast food menu items.

Effects of commercially available pneumatic compression on muscle glycogen recovery after exercise.

The purpose of this study was to investigate the effects of pneumatic compression pants on postexercise glycogen resynthesis. Active male subjects (n = 10) completed 2 trials consisting of a 90-minute glycogen depleting ride, followed by 4 hours of recovery with either a pneumatic compression device (PCD) or passive recovery (PR) in a random counterbalanced order. A carbohydrate beverage (1.8 g·kg bodyweight) was provided at 0 and 2 hours after exercise. Muscle biopsies (vastus lateralis) were obtained immediately and 4 hours after exercise for glycogen analyses. Blood samples were collected throughout recovery to measure glucose and insulin. Eight fingerstick blood samples for lactate were collected in the last 20 minutes of the exercise period and during the initial portion of the recovery period. Heart rate was monitored throughout the trial. During the PCD trial, subjects recovered using a commercially available recovery device (NormaTec PCD) operational at 0-60 and 120-180 minutes into recovery period. The same PCD was worn during the PR trial but was not turned on to create pulsatile pressures. There was no difference in muscle glycogen resynthesis during the recovery period (6.9 ± 0.8 and 6.9 ± 0.5 mmol·kg wet wt·h for the PR and PCD trials, respectively). Blood glucose, insulin, and lactate concentrations changed with respect to time but were not different between trials (p > 0.05). The use of PCD did not alter the rate of muscle glycogen resynthesis, blood lactate, or blood glucose and insulin concentrations associated with a postexercise oral glucose load.

Work patterns dictate energy demands and thermal strain during wildland firefighting.

OBJECTIVE: The purpose of this investigation was to characterize the effects of self-selected work activity on energy expenditure, water turnover, and thermal strain during wildland fire suppression. A secondary aim was to contrast current data with data collected 15 years ago using similar methods to determine whether job demands have changed. METHODS: Participants (n=15, 26±3 years, 179±6 cm, 78.3±8.6 kg) were monitored for 3 days for total energy expenditure, water turnover, core and chest skin temperature, physical activity, and heart rate. Participants arrived to the mobile laboratory each morning, submitted a nude weight, ingested a temperature transmitter, provided a urine sample, and were equipped with a physiological and activity monitor. Participants completed live wildland fire suppression during their work shifts. RESULTS: Mean core temperature was 37.6°±0.2°C, mean chest skin temperature was 34.1°±1.0°C, mean heart rate was 112±13 beats/min, and the mean physiological strain index score was 3.3±1.0. Wildland firefighters spent 49±8%, 39±6%, and 12±2% in the sedentary, light, and moderate-vigorous intensity categories, respectively. The mean total energy expenditure was 19.1±3.9 MJ/d, similar to 1997 (17.5±6.9 MJ/d). The mean water turnover in 2012 was 9.5±1.7 L/d, which was higher (P<.05) compared with 1997-98 (7.0±1.7 L/d). CONCLUSIONS: Wildland firefighters do not induce consistently high cardiovascular and thermal strain while completing arduous work in a hot environment despite fairly high chest skin temperatures. The total energy expenditure in the current study suggests job demands are similar to those of 15 years ago, while the increased water turnover may reflect a change in drinking habits.

The Effect of Environmental Temperature on Glucose and Insulin After an Oral Glucose Tolerance Test in Healthy Young Men.

OBJECTIVE: The purpose of this study was to compare glucose and insulin responses during an oral glucose tolerance test (OGTT) in cold (C), neutral (N), and hot (H) environments. METHODS: Eleven males completed three 4-hour climate-controlled OGTT trials (C, 7.2°C; N, 22°C; and H, 43°C). Participants remained semireclined for 60 minutes before ingesting a 1.8 g/kg glucose beverage. Skin and rectal core temperatures were continuously monitored. Blood was collected just before glucose ingestion (time 0) and at 15, 30, 60, 90, 120, and 180 minutes, and analyzed for serum glucose, insulin, hematocrit, and hemoglobin. Expired gases were collected upon entering the chamber (-60 minutes), before glucose ingestion (0 minutes), and at 60, 120, and 180 minutes to determine V(O2) and respiratory exchange ratio. RESULTS: Rectal core temperature was greater in the H condition compared with both C and N (P < .001). Rectal core temperature was not different between C and N, whereas skin temperature was different across all trials (H greater than N greater than C). The V(O2) was greater in C than in both H and N during all time points. Carbohydrate oxidation was greater in C compared with H and N (P < 0.001). Glucose was higher during H compared with C and N (P ≤ 0.002). Glucose was elevated in C compared with N. Insulin was higher in H compared with C (P = 0.009). Area under the curve for serum glucose was greater in H compared with C and N (P ≤ 0.001); however, there was no significant difference in area under the curve for insulin. CONCLUSIONS: These data indicate that after an OGTT, glucose and insulin are elevated in a hot environment.

A reduced core to skin temperature gradient, not a critical core temperature, affects aerobic capacity in the heat.

The purpose of this study was to determine the impact of the core to skin temperature gradient during incremental running to volitional fatigue across varying environmental conditions. A secondary aim was to determine if a "critical" core temperature would dictate volitional fatigue during running in the heat. 60 participants (n=49 male, n=11 female; 24±5 yrs, 177±11 cm, 75±13 kg) completed the study. Participants were uniformly stratified into a specific exercise temperature group (18 °C, 26 °C, 34 °C, or 42 °C) based on a 3-mile run performance. Participants were equipped with core and chest skin temperature sensors and a heart rate monitor, entered an environmental chamber (18 °C, 26 °C, 34 °C, or 42 °C), and rested in the seated position for 10 min before performing a walk/run to volitional exhaustion. Initial treadmill speed was 3.2 km h(-1) with a 0% grade. Every 3 min, starting with speed, speed and grade increased in an alternating pattern (speed increased by 0.805 km h(-1), grade increased by 0.5%). Time to volitional fatigue was longer for the 18 °C and 26 °C group compared to the 42 °C group, (58.1±9.3 and 62.6±6.5 min vs. 51.3±8.3 min, respectively, p<0.05). At the half-way point and finish, the core to skin gradient for the 18 °C and 26 °C groups was larger compared to 42 °C group (halfway: 2.6±0.7 and 2.0±0.6 vs. 1.3±0.5 for the 18 °C, 26 °C and 42 °C groups, respectively; finish: 3.3±0.7 and 3.5±1.1 vs. 2.1±0.9 for the 26 °C, 34 °C, and 42 °C groups, respectively, p<0.05). Sweat rate was lower in the 18 °C group compared to the 26 °C, 34 °C, and 42 °C groups, 3.6±1.3 vs. 7.2±3.0, 7.1±2.0, and 7.6±1.7 g m(-2) min(-1), respectively, p<0.05. There were no group differences in core temperature and heart rate response during the exercise trials. The current data demonstrate a 13% and 22% longer run time to exhaustion for the 18 °C and 26 °C group, respectively, compared to the 42 °C group despite no differences in beginning and ending core temperatures or baseline 3-mile run time. This capacity difference appears to result from a magnified core to skin gradient via an environmental temperature advantageous to convective heat loss, and in part from an increased sweat rate.

Seasonal heat acclimatization in wildland firefighters.

The purpose of this study was to determine changes in physiological markers of heat acclimatization across a 4-month wildland fire season. Wildland firefighters (WLFF) (n=12) and non-WLFF (n=14) were assessed pre- and post-season for body mass, percent body fat, and peak VO2. Both groups completed a 60-min heat stress trial (walking at 50% of peak VO2) in a climate controlled chamber (43.3 °C, 33% RH) pre and post-fire season (May through September). During the trials, core (Tc) and skin (Tsk) temperatures, heart rate (HR), physiological strain index (PSI), and rating of perceived exertion (RPE) were measured. There were no differences pre or post-season between the WLFF and non-WLFF groups in body mass, percent body fat, or peak V.O2. During the 73 days where the WLFF were involved in direct wildland fire suppression, daily high temperature for the WLFF was higher compared to the non-WLFF, 30.6 ± 5.4 °C and 26.9 ± 6.1 °C, respectively, p<0.05. Tc was lower at post-season compared to pre-season (p<0.05) for the WLFF at 30, 45, and 60 min (pre 30, 45, and 60: 37.9 ± 0.3, 38.3 ± 0.3 and 38.5 ± 0.3 °C, respectively; post 30, 45, and 60: 37.8 ± 0.3, 38.1 ± 0.3 and 38.2 ± 0.4 °C, respectively). For WLFF, PSI was lower (p<0.05) at 15, 30, 45, and 60 min at post-season compared to pre-season (4.2 ± 0.7, 5.6 ± 0.9, 6.5 ± 0.9, and 7.1 ± 1.1 for 15, 30, 45, and 60 min pre-season, respectively; 3.6 ± 0.8, 4.9 ± 1.0, 5.7 ± 1.2, 6.3 ± 1.3 for 15, 30, 45, and 60 min post-season, respectively). For WLFF, RPE was lower during the post-season trial at 30, 45, and 60 min (pre 30, 45, and 60: 11.7 ± 1.4, 12.3 ± 1.2, and 13.5 ± 1.4, respectively; post 30, 45, and 60: 10.7 ± 1.2, 11.3 ± 1.3, and 11.9 ± 1.5, respectively), p<0.05. There were no differences between pre and post-season for the non-WLFF for Tc and PSI, but RPE was lower at 15 min during the pre-season trial. WLFFs demonstrated significant decreases in Tc, PSI, and RPE during controlled heat stress after the season. Since an age and fitness-matched control group experienced no indication of heat acclimatization, it is suggested that the long-term occupational heat exposure accrued by the WLFFs was adequate to incur heat acclimatization.

Human skeletal muscle mRNAResponse to a single hypoxic exercise bout.

BACKGROUND: The ability to physically perform at high altitude may require unique strategies to acclimatize before exposure. The effect of acute hypoxic exposure on the metabolic response of the skeletal muscle may provide insight into the value of short-term preacclimatization strategies. OBJECTIVE: To determine the human skeletal muscle response to a single acute bout of exercise in a hypoxic environment on metabolic gene expression. METHODS: Eleven recreationally active male participants (24 ± 4 years, 173 ± 20 cm, 82 ± 12 kg, 15.2 ± 7.1% fat, 4.0 ± 0.6 L/min maximal oxygen consumption) completed two 1-hour cycling exercise trials at 60% of peak power followed by 4 hours of recovery in ambient environmental conditions (975 m) and at normobaric hypoxic conditions simulating 3000 m in a randomized counterbalanced order. Muscle biopsies were obtained from the vastus lateralis before exercise and 4 hours after exercise for real-time polymerase chain reaction analysis of select metabolic genes. RESULTS: Gene expression of hypoxia-inducible factor 1 alpha, cytochrome c oxidase subunit 4, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, hexokinase, phosphofructokinase, mitochondrial fission 1, and mitofusin-2 increased with exercise (P < .05) but did not differ with hypoxic exposure (P > .05). Optic atrophy 1 did not increase with exercise or differ between environmental conditions (P > .05). CONCLUSIONS: The improvements in mitochondrial function reported with intermittent hypoxic training may not be explained by a single acute hypoxic exposure, and thus it appears that a longer period of preacclimatization than a single exposure may be required.

Substrate use and biochemical response to a 3,211-km bicycle tour in trained cyclists.

The purpose of this study was to assess the physiological adaptations in physically fit individuals to a period of intensified training. Ten trained males cycled outdoors ~170 km day(-1) on 19 out of 21 days. Expired gas was collected on days 1 and 21 during maximal graded exercise and used for the determination of gross efficiency and whole body substrate use. Muscle biopsies were obtained before and after exercise on days 2 and 22 for the determination of mtDNA/gDNA ratio, gene expression, metabolic enzyme activity and glycogen use. Muscle glycogen before and after exercise, fat oxidation, and gross efficiency increased, carbohydrate oxidation decreased (p < 0.05), and VO(2max) did not change over the 21 days of training. Citrate synthase (CS), ß-hydroxyacyl CoA dehydrogenase (ß-HAD) and cytochrome c oxidase (COX) enzyme activity did not change with training. CS and ß-HAD mRNA did not change with acute exercise or training. COX (subunit IV) mRNA increased with acute exercise (p < 0.05) but did not change over the 21 days. PGC-1α mRNA increased with acute exercise, but did not increase to the same degree on day 22 as it did on day 2 (p < 0.05). UCP3 mRNA decreased with training (p < 0.05). Acute exercise caused an increase in mitofusin2 (MFN2) mRNA (p < 0.05) and a trend for an increase in mtDNA/gDNA ratio (p = 0.057). However, training did not affect MFN2 mRNA or mtDNA/gDNA ratio. In response to 3,211 km of cycling, changes in substrate use and gross efficiency appear to be more profound than mitochondrial adaptations in trained individuals.

Applications of real-time thermoregulatory models to occupational heat stress: validation with military and civilian field studies.

A real-time thermoregulatory model using noninvasive measurements as inputs was developed for predicting physiological responses of individuals working long hours. The purpose of the model is to reduce heat-related injuries and illness by predicting the physiological effects of thermal stress on individuals while working. The model was originally validated mainly by using data from controlled laboratory studies. This study expands the validation of the model with field data from 26 test volunteers, including US Marines, Australian soldiers, and US wildland fire fighters (WLFF). These data encompass a range of environmental conditions (air temperature: 19-30° C; relative humidity: 25-63%) and clothing (i.e., battle dress uniform, chemical-biological protective garment, WLFF protective gear), while performing diverse activities (e.g., marksmanship, marching, extinguishing fires, and digging). The predicted core temperatures (Tc), calculated using environmental, anthropometric, clothing, and heart rate measures collected in the field as model inputs, were compared with subjects' Tc collected with ingested telemetry temperature pills. Root mean standard deviation (RMSD) values, used for goodness of fit comparisons, indicated that overall, the model predictions were in close agreement with the measured values (grand mean of RMSD: 0.15-0.38° C). Although the field data showed more individual variability in the physiological data relative to more controlled laboratory studies, this study showed that the performance of the model was adequate.

Water turnover and core temperature on Mount Rainier.

OBJECTIVE: Hydration is an important logistical consideration for persons performing in austere environments because water demands must be balanced with the burden of carrying water. METHODS: Seven novice climbers participated in a study to determine the hydration kinetics and core temperatures associated with a successful summit of Mount Rainier. Ingestible radio-equipped thermometer capsules were swallowed to monitor core temperature, and an oral dose of deuterium (0.12 ± 0.02 g·kg⁻¹ body weight) was administered to determine hydration kinetics. RESULTS: Mean core temperature throughout the 5.5-hour climb to Camp Muir (3000 m) was 37.6 ± 0.3°C. Water turnover was 95.0 ± 17.5 mL·kg⁻¹·24 h⁻¹ over the duration of the 43-hour study. There was a trend for reduced body mass from before (75.9 ± 13.0 kg) to after (74.8 ± 12.5 kg) the climb (P = .06), and urine specific gravity increased from before (1.013 ± 0.002) to after (1.022 ± 0.006) the climb (P = .004). CONCLUSIONS: Hydration demands of climbing Mount Rainier are highly elevated despite modest fluctuations in core temperature. Participants experienced hypohydration but were able to maintain sufficient hydration to successfully summit Mount Rainier and return home safely.

Factors of trainability and predictability associated with military physical fitness test success.

Cuddy, JS, Slivka, DR, Hailes, WS, and Ruby, BC. Factors of trainability and predictability associated with military physical fitness test success. J Strength Cond Res 25(12): 3486-3494, 2011-The purpose of this study was to determine the trainability of college-aged men using varied training programs and to assess factors associated with successfully passing a Special Operations Forces (SOF) physical fitness test (PFT). One hundred thirty-five male subjects were stratified into 3 training groups (run focused, calisthenic focused, or combined run and calisthenic) and were trained 3 times·per week for 12 weeks. Body composition and accelerometer activity patterns were measured pretraining and posttraining. The PFT performance (pull-ups, sit-ups, push-ups, and 1.5-mile run time) was measured weekly throughout the study period. The subjects exhibited reduced body fat (18.4 ± 7.7 to 16.9 ± 7.3), increased fat-free mass (66.1 ± 8.2 to 67.4 ± 7.9), reduced fat mass (15.8 ± 9.2 to 14.6 ± 8.9) from pretraining to posttraining, respectively (p < 0.05). All groups improved in each component of PFT performance with training (p < 0.05). There was a significant 20 ± 35% increase in 6-day average daily activity for the run-focused training group from pretraining and posttraining. The key indicators of a candidate's potential to successfully reach SOF PFT standards (in 12 weeks) were determined to be as follows: enter the pipeline being able to run 2.4 km in ≤10:41 minutes, have a body fat percentage of ≤12.9%, and participate in a minimum of 30 min·d of vigorous physical activity. Training an individual's relative run or calisthenic deficiency did not prove to be a better training approach compared with a program that emphasizes training both running and calisthenic activities.

High work output combined with high ambient temperatures caused heat exhaustion in a wildland firefighter despite high fluid intake.

The purpose of this case study is to examine the physiological/behavioral factors leading up to heat exhaustion in a male wildland firefighter during wildland fire suppression. The participant (24 years old, 173 cm, 70 kg, and 3 years firefighting experience) experienced heat exhaustion following 7 hours of high ambient temperatures and arduous work on the fire line during the month of August. At the time of the heat-related incident (HRI), core temperature was 40.1 °C (104.2 °F) and skin temperature was 34.4 °C (93.9 °F). His work output averaged 1067 counts·min(-1) (arbitrary units for measuring activity) for the 7 hours prior to the HRI, a very high rate of work over an extended time period during wildfire suppression. In the 2.5 hours leading up to the heat incident, he was exposed to a mean ambient temperature of 44.6 °C (112.3 °F), with a maximum temperature of 59.7 °C (139.5 °F). He consumed an average of 840 mL·h(-1) in the 7 hours leading up to the incident and took an average of 24 ± 11 drinks·h(-1) (total of 170 drinks). The combined effects of a high work rate and high ambient temperatures resulted in an elevated core temperature and a higher volume and frequency of drinking than typically seen in this population, ultimately ending in heat exhaustion and removal from the fire line. The data demonstrate that heat-related incidents can occur even with aggressive fluid intake during wildland fire suppression.

Glycogen levels in wildland firefighters during wildfire suppression.

OBJECTIVE: The purpose of this project was to determine the effects of wildfire suppression on muscle glycogen utilization in wildland firefighters (WLFFs). METHODS: Wildland firefighters (n = 11) participated in the study. Muscle biopsies were obtained from the vastus lateralis pre- and post-work shift. Activity patterns were measured using an Actical activity monitor positioned on the chest. Food was consumed ad libitum and recorded using a food log and interview. Differences were analyzed using paired samples t-tests and relationships were assessed using Pearson r correlation coefficients. A significance level of p < .05 was set. RESULTS: Body weight was similar pre- to post-work shift (85.9 ± 9.1 and 85.6 ± 8.8 kg, respectively). Muscle glycogen decreased from pre- to post-work shift, 101 ± 7 to 80 ± 5 mmol/kg wet wt, p < .05. Average activity counts were 175 ± 60 counts/min. Mean percent of time spent in each intensity category included: sedentary (74 ± 7%), light (21 ± 5%), and moderate/vigorous (5 ± 2%). There was a significant relationship between minutes completing vigorous activity and glycogen utilization (r = -.76, p < .05), and between minutes spent completing vigorous activity and pre-shift glycogen content (r = .79, p < .05). Kilocalorie intake during the work shift was 9.2 ± 2.9 MJ/d (2195 ± 699 kcal/d). CONCLUSIONS: This study demonstrates the variety of self-selected nutritional and activity habits of WLFFs, and emphasizes the relationships between moderate/vigorous activity and muscle glycogen. The current data suggest that the food provided was adequate to maintain muscle glycogen levels pre- to post-work shift.

Effects of 21 days of intensified training on markers of overtraining.

The purpose of this study was to impose a period of quantifiable intensified training to determine if commonly used diagnostic markers of overtraining parallel changes in physical performance and thus overtraining status. Eight trained male cyclists (24 ± 1 years, 71 ± 3 kg, VO2peak = 4.5 ± 0.1·L·min⁻¹) performed 21 days (3,211 km) of intensified training in the field where volume and intensity were increased over normal training. Salivary IgA, testosterone, and cortisol, 1-hour time trial performance, heart rate response, and profile of mood states (POMS) were collected and analyzed throughout the 21-day training period. The POMS category vigor declined from day 1 to day 4 and remained lower throughout (p < 0.05). There were no other statistical changes in overtraining parameters. However, individuals who demonstrated 2 or more symptoms of overtraining at any point throughout the 21 days were considered symptomatic and had a lower (p < 0.05) VO2peak (4.2 ± 0.1·vs. 4.7 ± 0.1 L·min⁻¹) and lower (p < 0.05) average workload during the initial 1-hour time trial (253 ± 5 vs. 288 ± 14 W). Interestingly, the 1-hour time trial power in these individuals with symptoms of overtraining did not decline (p > 0.05). These data demonstrate that markers of overtraining do not parallel a decrease in performance and should be interpreted with caution.

Effects of modafinil and sleep loss on physiological parameters.

OBJECTIVE: The purpose of this study was to determine the effects of modafinil administration on physical performance, thermoregulation, and total energy expenditure (TEE) during continued wakefulness in Air Force operators. METHODS: Participants (N = 12) were randomly assigned to the modafinil or placebo group. Participants performed physical performance and testing during 72 hours of wakefulness. RESULTS: There were no differences between groups for physical performance. Oral temperature was higher for modafinil compared to placebo (36.5 +/- 0.2 degrees C versus 36.3 +/- 0.2 degrees C for modafinil and placebo, respectively, p < 0.05). Daily water turnover (8.8 +/- 1.0 L x day(-1) and 9.0 +/- 1.5 L x day(-1)) and total energy expenditure (19.4 +/- 3.7 and 19.9 +/- 2.1 MJ) were similar between the modafinil and placebo groups, respectively. CONCLUSION: modafinil did not improve physical performance. Despite elevating oral temperature, water turnover and TEE were similar between treatments. These findings suggest it is unnecessary for operators taking modafinil to carry additional fluids and/or food.

Supplemental feedings increase self-selected work output during wildfire suppression.

PURPOSE: The purpose of this study was to determine the impact of supplemental feeding strategies on self-selected activity during wildland fire suppression. METHODS: Seventy-six wildland firefighters were studied in three experiments for three fire seasons. During the first two seasons, subjects consumed, in addition to their sack lunch, 1) liquid carbohydrate (CHO) (200 mL.h, 20% CHO (40 g.h, 160 kcal.h)) or placebo (PLA) every hour, or 2) liquid CHO (200 mL.h, 20% CHO (40 g.h, 160 kcal.h) every even hour and solid CHO (25 g of CHO, 10 g of protein, 2 g of fat, and 160 kcal.h) every odd hour, or PLA, using counterbalanced crossover designs. During the third season, subjects consumed their sack lunch halfway through their workday, or shift food items of approximately 150-400 kcal at 90-min intervals after breakfast in a randomized crossover design (isocaloric intake, 1534+/-265 kcal per workday). Work output was monitored using CSA and MiniMitter actigraphy units. RESULTS: During the liquid CHO trials, subjects consuming CHO demonstrated significantly higher average activity counts throughout the day compared with PLA (50,262+/-36,560 and 40,159+/-35,969 counts per hour for 12 h for the CHO and PLA trials, respectively; P<0.05). For the liquid+solid CHO trials, subjects consuming CHO demonstrated higher average activity counts per minute 2 h before lunch and the last 4 h of the workday compared with PLA (P<0.05). For the sack lunch and shift food trials, subjects consuming shift foods demonstrated higher average counts per minute during the final 2 h compared with those consuming sack lunch (521+/-421 vs 366+/-249 counts per minute during 2 h; P<0.05). CONCLUSION: Liquid and/or solid supplemental CHO and regular feedings increased self-selected work rates during wildland fire suppression, particularly during the latter hours of the workday.

Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat.

BACKGROUND: Heat stress down-regulates mitochondrial function, while carbohydrate supplementation attenuates the exercise induced stimulation of mitochondrial biogenesis in humans. The effects of exogenous carbohydrate during exercise in the heat on metabolic mRNA have not been investigated in humans. The purpose of this study was to determine the impact of exercise with and without carbohydrate supplementation on skeletal muscle metabolic response in the heat. METHODS: Eight recreationally active males (4.05 ± 0.2 L.min-1) completed 2 trials which included 1 hr of cycling at 70% workload max and 3 hr recovery in a hot environment. Both trials were conducted in a climate controlled environmental chamber (38°C and 40% RH). The trials differed by the consumption of either a 6% carbohydrate (CHO) containing beverage (8 ml.kg-1.hr-1) or placebo (P) during exercise in random order. Muscle biopsies were obtained from the vastus lateralis before exercise, immediately post-exercise and at the end of the 3 hr recovery period. Muscle was analyzed for muscle glycogen and mRNA related to metabolic and mitochondrial development (MFN2, PGC-1α, GLUT4, UCP3). Expired gases were measured to determine whole body substrate use during exercise. RESULTS: Carbohydrate oxidation and muscle glycogen utilization did not differ between trials, whereas fat oxidation was elevated during exercise in P. Exercise caused an increase in PGC-1α, and GLUT4 (P < 0.05) independent of exogenous carbohydrate provision. Carbohydrate consumption attenuated the mRNA response in UCP3 (P < 0.05). CONCLUSIONS: This study indicates that the provision of exogenous carbohydrate attenuates the stimulation of mRNA expression of UCP3 following exercise in the heat.

Skin temperature and heart rate can be used to estimate physiological strain during exercise in the heat in a cohort of fit and unfit males.

OBJECTIVE: To evaluate the previously developed physiological strain index (PSI) model using heart rate and skin temperature to provide further insight into the detection and estimation of thermal and physiological heat strain indices. A secondary aim was to characterize individuals who excel in their performance in the heat. METHODS: 56 male participants completed 2 walking trials (3.5 miles per hour, 5% grade) in controlled environments of 43.3 °C and 15.5 °C (40% humidity). Core and skin temperature, along with heart rate and PSI, were continually monitored during exercise. Participants completed a physical fitness test. RESULTS: The logistic regression model exhibited 4 false positives and 1 false negative at the 40% decision boundary. The "Not at Risk" group (N = 33) had higher body weight (84 ± 13 vs. 77 ± 10 kg, respectively) compared to the "At Risk" (N = 23) group, p < 0.05. The "Not at Risk" group had a faster 3-mile run time compared to the "At Risk" group (21:53 ± 3:13 vs. 25:16 ± 2:37, respectively), p < 0.05. During the Heat Trial, the "At Risk" group had a higher rating of perceived exertion at 60 and 90 minutes compared to the "Not at Risk" group (13.5 ± 2.8 vs. 11.5 ± 1.8 and 14.8 ± 3.2 vs. 12.2 ± 2.0 for "At Risk" vs. "Not at Risk" at 60 and 90 minutes, respectively), p < 0.05. CONCLUSIONS: The previously developed model relating heart rate and skin temperature to PSI is highly accurate at assessing heat risk status. Participants classified as "At Risk" had lower physical performance scores and different body weights compared to the "Not at Risk" group and perceived themselves as working harder during exercise in the heat.

Accelerometry and salivary cortisol response during Air Force Special Tactics Officer selection.

BACKGROUND: Special Tactics Officer (STO) selection is conducted to select officers to enter the combat controller training pipeline. The aims were to determine physical activity patterns, estimate energy expenditure, and identify whether return and/or unsuccessful candidates demonstrated differences in cortisol responses compared to non-selected and/or first-time attendees. METHODS: Participants completed the STO selection, consisting of 5 days of physical and mental challenges. Participants were equipped with ActiCals®, and saliva samples were collected throughout the STO selection. RESULTS: Average activity counts were 684 ± 200 counts∙min-1, with no group differences. Estimated energy expenditure was 4,105 ± 451 kcal∙day-1. Cortisol was elevated following extended physical training but returned to baseline during rest. Return candidates had significantly lower cortisol responses compared to first-timers, 0.43 ± 0.06 µg∙dl-1 versus 0.76 ± 0.18 µg∙dl-1, respectively, p < 0.05. CONCLUSIONS: An individual's salivary cortisol response to the stresses incurred during the STO selection has the potential to be incorporated into the entire picture of a candidate's performance and ability to handle stress.