Interrelationship between platelets and BUN in postmenopausal patients with coronary heart disease: findings of the NHANES from 2003 to 2016.

OBJECTIVE: During menopause, women are more likely to develop coronary heart disease (CHD) due to the significant changes in body metabolism brought on by the loss of estrogen. The purpose of this study was to investigate the independent association between platelet parameters and blood urea nitrogen (BUN) in postmenopausal patients with coronary artery disease in order to clarify the function performed by platelet parameters and BUN in thrombosis. PATIENTS AND METHODS: We took information from the NHANES between 2003 and 2016. Platelet count (PC), mean platelet volume (MPV), and PC/MPV were the independent variables, BUN was the dependent variable, and age, race, marital status, body mass index (BMI), inflammation indicators, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were the covariates. RESULTS: BUN decreased with increasing PC in postmenopausal heart disease patients after controlling for other factors. When PC/MPV was less than 30.5, there was a strong negative correlation with BUN. In addition, there was a strong positive correlation with BUN when MPV was less than 9.3 fL. CONCLUSIONS: The findings of this study will contribute to a better understanding of the mechanisms underlying thrombosis in postmenopausal women with CHD and offer fresh perspectives on how to create novel antithrombotic medications for an aging population.

MiR-34a-5p directly targeting TRIM44 affects the biological behavior of ovarian cancer cells.

OBJECTIVE: This research intended to explore the expression and molecular mechanism of miR-34a-5p and Tripartite motif-containing protein 44 (TRIM44) in ovarian cancer (OC). PATIENTS AND METHODS: Tissue and serums of OC patients were collected, and miR-34a-5p and TRIM44 in serum and tissue were tested by Real-time quantitative PCR (qRT-PCR). In vitro cell experiment was constructed. Methyl Thiazolyl Tetrazolium (MTT), transwell, and flow cytometry were applied to test the proliferation, migration, invasion, and apoptosis. Western blot was performed to explore TRIM44 and epithelial-mesenchymal transition (EMT) proteins. RESULTS: MiR-34a-5p was low expressed, while TRIM44 was highly expressed, which was related to Federation International of Gynecology and Obstetrics (FIGO) staging and lymph node metastasis. The receiver operating characteristic curve (ROC) revealed that the Area Under Curve (AUC) of miR-34a-5p and TRIM44 were 0.885 and 0.868, respectively. An evident increase of miR-34a-5p or decrease of TRIM44 could inhibit OC malignant behaviors, and E-cadherin increased while N-cadherin and Fibronectin decreased. The knockdown of miR-34a-5p or overexpression of TRIM44 could inhibit the malignant behavior of ovarian cancer cells, hinder the malignant behaviors, and reduce E-cadherin, while N-cadherin and Fibronectin protein was enhanced. Co-transfection found that overexpression of miR-34a-5p eliminated the biological behaviors of OC cells by TRIM44. CONCLUSIONS: MiR-34a-5p and TRIM44 can be used as diagnostic markers for OC. MiR-34a-5p can act in biological behaviors by targeting TRIM44 in OC.