RESUMO
Twenty-eight warmblood mares were monitored during their late pregnancy in the Teaching Hospital of Ghent University. The reliability of two commercial assays (enzyme immunoassay and glutaraldehyde coagulation test) used for determining the IgG concentrations of their newborn foals was tested. Mammary secretions were examined at the time of foaling (T0), and then 4 (T1) and 8 (T2) hours after foaling by refractometry and electrophoresis. The foals' blood IgG levels were measured at T1 and T2 as a routine clinical diagnostic examination using two different commercial test kits (SNAP Foal Ig and Gamma-Check E) and T0, T1 and T2 samples were stored (at -18 °C) for immunoglobulin (Ig) determination by electrophoresis. Differences between the results of refractometry and electrophoresis occurred in 27.8% of the colostrum analyses. Some serum IgG could be detected immediately post partum (T0) in 75% of the foals, and 42.82% of the newborn foals acquired a serum concentration of more than 800 mg/dl IgG within 8 h of birth. Compared to the electrophoresis, the glutaraldehyde test scored better (85%) than the enzyme immunoassay (74%), although both are accurate and safe to use since they clearly distinguish between safe and unsafe IgG concentrations.
Assuntos
Testes de Coagulação Sanguínea/veterinária , Testes Diagnósticos de Rotina/veterinária , Eletroforese/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Refratometria/veterinária , Animais , Animais Recém-Nascidos , Testes de Coagulação Sanguínea/métodos , Colostro/química , Testes Diagnósticos de Rotina/métodos , Eletroforese/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Glutaral/química , Cavalos , Imunoglobulina G/sangue , Refratometria/métodos , Reprodutibilidade dos TestesRESUMO
Ascaris suum and Macracanthorhynchus hirudinaceus cause a large loss of yield in farm animals as well as in free-living and captive wild boar herds, thereby causing economic damage. This study compared A. suum and M. hirudinaceus infections in free-ranging and captive wild boars (Sus scrofa) in Hungary. The authors measured the A. suum and M. hirudinaceus infections of a 248-hectare wild boar garden and an 11,893-hectare free-living wild boar herd in the sample area. In all cases, samples were collected from shot wild boars. In total, 216 wild boars were examined from June 2015 to June 2023 in Hungary. Of the 173 dissected wild boars from the wild, 57 (32.9%) were infected with A. suum, while 30 (69.8%) of the 43 individuals from the captive area were infected. The prevalence of M. hirudinaceus in the free-living area population was 9.25% (16 wild boars), while that of the captive population was 34.89% (15 wild boars). In the case of the examined helminths, the captive herd was 36.9% more infected than the herd living in the open area.