RESUMO
In the DA-to-Lewis renal allograft model, donor whole blood enhanced renal allograft survival (14.5 +/- 7.6 days versus 6.9 +/- 0.6 days in controls [P less than 0.01]). The effect of individual cell components given in numbers equivalent to those present in the enhancing volumes of donor whole blood was studied. Immunization with donor red cells alone produced greater enhancement than that produced by whole blood (36.14 +/- 19.5 days [P less than 0.01]). B lymphocytes also enhanced allograft survival (16.0 +/- 3.9 days [P less than 0.01]). Although slight enhancement was observed with platelets (8.5 +/- 0.6 days) and 10(5) dendritic cells (8.4 +/- 0.5 days), in terms of allograft function dendritic cell immunization produced evidence of dose-dependent accelerated rejection. A similar finding was obtained with donor T cell immunization. Donor plasma had no effect. We conclude that, although donor blood has an overall enhancing effect on renal allograft survival in this model, the sensitizing and enhancing effects can be ascribed to individual types of cells.
Assuntos
Transfusão de Sangue , Facilitação Imunológica de Enxerto , Imunização , Transplante de Rim , Transfusão de Linfócitos , Animais , Soro Antilinfocitário/biossíntese , Transfusão de Sangue/métodos , Nitrogênio da Ureia Sanguínea , Transfusão de Eritrócitos , Sobrevivência de Enxerto , Linfócitos/classificação , Masculino , Modelos Biológicos , Ratos , Ratos Endogâmicos Lew , Linfócitos T/transplanteAssuntos
Transfusão de Sangue , Transplante de Rim , Formação de Anticorpos , Citotoxicidade Imunológica , Teste de Histocompatibilidade/métodos , Humanos , Idiótipos de Imunoglobulinas/análise , Cuidados Pré-Operatórios , Linfócitos T Reguladores/imunologia , Reação Transfusional , Uremia/imunologiaRESUMO
A prospective study of serial serum C-reactive protein (CRP) concentrations was made on 21 patients who had received renal allografts. CRP was raised during 27 of 32 rejection episodes and in all of five episodes of rejection associated with infection. CRP values were persistently elevated in three irreversible rejection episodes. Significantly raised CRP concentrations were documented in 14 of 20 episodes of infection. In some cases CRP was a predictive indicator of rejection or infection. In all cases of infection or rejection associated with a rise in CRP, CRP values fell following successful treatment with appropriate agents. Serial CRP measurement used in conjunction with other clinical and biochemical parameters appears to be valuable clinically following renal transplantation.