Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island.

A bacterial strain, 17J42-1T, was isolated from a soil sample collected on Jeju Island, Republic of Korea. Colonies grown on R2A agar were pink in color, and cells were Gram-stain negative, short and rod-shaped. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Methylobacterium in the family Methylobacteriaceae, with high levels of 16S rRNA sequence similarity shared with Methylobacterium oxalidis 35aT (98.6%), Methylobacterium jeotgali S2R03-9T (97.5%), and Methylobacterium soli YIM 48816T (97.3%). Cells grew at 15-35 °C, pH 5-9, and in the presence of 0-1.0% NaCl. The genomic G + C content was 70.2 mol% based on the whole genome analysis. The predominant respiratory quinone was ubiquinone Q-10, the major fatty acid was C18:1ω7c (85.3%), and the major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The phenotypic and chemotaxonomic data support the affiliation of strain 17J42-1T with the genus Methylobacterium. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbors was lower than 38%. The OrthoANI and dDDH values between strain 17J42-1T and the closest type strain Methylobacterium oxalidis NBRC 107715T were calculated to be 85.9% and 30.6%, respectively. The results of 16S rRNA gene sequence similarity analysis, DNA-DNA hybridization analysis, and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17J42-1T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium segetis sp. nov. is proposed. The type strain is 17J42-1T (= KCTC 62267T = JCM 33059T).

Lysobacter terrigena sp. nov., isolated from a Korean soil sample.

A bacterial strain isolated from a soil collected in Jeju Island, designated as 17J7-1T, was Gram-negative, rod-shaped, yellow colored, and motile by gliding. This strain was able to grow at temperature range from 10 to 42 °C, pH 7-9, and tolerated up to 1% NaCl. Analysis of 16S rRNA sequence identified strain 17J7-1T as a member of the genus Lysobacter with close sequence similarity with Lysobacter mobilis 9NM-14T (97.4%), Lysobacter xinjiangensis RCML-52T (97.0%), and Lysobacter humi FJY8T (96.9%). The genomic DNA G + C content of the isolate was 67.9 mol%. DNA-DNA relatedness between strain 17J7-1T and L. mobilis, L. humi, and L. xinjiangensis were 42.3%, 39.5%, and 35.8%, respectively, clearly showing that the isolate is distinct from its closest phylogenetic neighbors in the genus Lysobacter. Average nucleotide identity (ANI) and digital DNA-DNAhybridization (dDDH) values between strain 17J7-1T and L. enzymogenes ATCC 29487T, the type species of this genus, and several other close Lysobacter species were less than 77% and 22%, respectively. Major fatty acids were C16:0 iso (29.8%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl; 20.1%), and C15:0 iso (17.7%). The predominant respiratory quinone was ubiquinone Q-8 and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. In the light of the polyphasic evidence accumulated in this study, strain 17J7-1T is considered to represent a novel species in the genus Lysobacter, for which name Lysobacter terrigena sp. nov. is proposed. The type strain is 17J7-1T (= KCTC 62217T = JCM 33057T).

Lysobacter segetis sp. nov., Isolated from Soil.

A Gram-negative, aerobic, motile by gliding, rod-shaped bacterium, strain 17J68-2T, was isolated from a soil sample taken from Jeju Island, Republic of Korea. The isolate displayed high 16S rRNA gene sequence similarity to the members of the genus Lysobacter in the family Lysobacteraceae, with Lysobacter humi FJY8T (98.4% similarity), Lysobacter xinjiangensis RCML-52T (98.3%), and Lysobacter mobilis 9NM-14T (98.1%) as closest phylogenetic neighbors. Growth of strain 17J68-2T occurred at 15-42 °C, pH 7-8, and in the presence of 0-1.0% NaCl. Draft genome was 2.94 Mb in size with G+C content of 70.5 mol%. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. Ubiquinone Q-8 was the predominant respiratory quinone and the major fatty acids were C16:0 iso (39.4%), summed feature 3 (C16:1ω7c/C16:1ω6c) (6.6%), C11:0 iso 3-OH (6.4%), C15:0 iso (6.4%), and C16:1 iso H (6.2%). The DNA-DNA relatedness between strain 17J68-2T and L. humi, L. xinjiangensis, and L. mobilis were 39.9, 39.4, and 25.3%, respectively. From these results, it is concluded that the novel isolate possesses sufficient characteristics to differentiate it from the most closely affiliated Lysobacter species, and strain 17J68-2T represents a novel species of the genus Lysobacter, for which the name Lysobacter segetis sp. nov. (=KCTC 62237T = JCM 33058T) is proposed.