RESUMO
The conditions of Moscow 2010 summer heat wave were simulated in an accommodation module. Six healthy men aged from 22 to 46 years stayed in the module for 30 days. Measurements of gene expression in peripheral blood leukocytes before, during and 3 day after simulated heat wave were performed using qRT-PCR. We observed a shift in the expression level of certain genes after heat exposure for a long time, and rapid return to the initial level, when volunteers leaved the accommodation module. Eight genes were chosen to form the "heat expression signature". EGR2, EGR3 were upregulated in all six volunteers, EGR1, SIRT1, CYP51A1, MAPK9, BAG5, MNDA were upregulated in 5 volunteers.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Antígenos de Diferenciação Mielomonocítica/genética , Fatores de Transcrição de Resposta de Crescimento Precoce/genética , Proteína Quinase 9 Ativada por Mitógeno/genética , Sirtuína 1/genética , Esterol 14-Desmetilase/genética , Termotolerância/genética , Fatores de Transcrição/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Adulto , Antígenos de Diferenciação Mielomonocítica/metabolismo , Fatores de Transcrição de Resposta de Crescimento Precoce/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Estudo de Associação Genômica Ampla , Voluntários Saudáveis , Temperatura Alta , Humanos , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , Proteína Quinase 9 Ativada por Mitógeno/metabolismo , Sirtuína 1/metabolismo , Esterol 14-Desmetilase/metabolismo , Fatores de Transcrição/metabolismo , TranscriptomaRESUMO
M. gallisepticum genome fragment carrying complete coding sequence for ATP-binding subunit of topoisomerase II (topIIB), partial coding sequence for N-terminal part of A-subunit of topoisomerase II and region upstream of topIIB gene (open reading frame encoding 99 amino acids) was sequenced. The nucleotide sequence of topIIB has significant homology with previously reported gyrase genes and parE gene of E. coli. No protein sequence significantly similar to the open reading frame upstream from the gene topIIB was found in GeneBank data.
Assuntos
Trifosfato de Adenosina/metabolismo , DNA Topoisomerases Tipo II/genética , Genes Bacterianos , Mycoplasma/genética , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Clonagem Molecular , DNA Topoisomerases Tipo II/metabolismo , DNA Bacteriano , Dados de Sequência Molecular , Homologia de Sequência de AminoácidosRESUMO
Carney complex is an autosomic dominant disorder initially described as the association of cardiac myxomas, spotty skin pigmentation and endocrine overactivity and considered as a multiple neoplasia and lentiginosis syndrome. Mutations in the tumor suppressor gene PRKAR1A, coding for the type 1-alpha regulatory subunit of cAMP-depended protein kinase A have been previously identified in about half of the Carney complex kindreds. In this paper we report identification of the molecular defect in PRKARIA gene in two Carney complex patients. A new mutation (403delAC) located in a 3rd exon of PRKARIA gene has been observed in one case, and a previously described mutation in exon 7 (847delTC) in the second case.
Assuntos
Proteínas Quinases Dependentes de AMP Cíclico/genética , Neoplasias Cardíacas/genética , Neoplasia Endócrina Múltipla/genética , Mixoma/genética , Transtornos da Pigmentação/genética , Adolescente , Adulto , Subunidade RIalfa da Proteína Quinase Dependente de AMP Cíclico , Genes Supressores de Tumor , Humanos , Masculino , Mutação , Linhagem , SíndromeAssuntos
Proteínas de Bactérias/genética , Cromossomos Bacterianos/genética , Proteínas de Escherichia coli , Mycoplasma/genética , Óperon , Proteínas Ribossômicas/genética , Sequência de Aminoácidos , Aminopeptidases/genética , Sequência de Bases , DNA Bacteriano/química , DNA Bacteriano/genética , L-Lactato Desidrogenase/química , L-Lactato Desidrogenase/genética , Metionil Aminopeptidases , Dados de Sequência Molecular , Mycoplasma/enzimologia , Mapeamento Físico do Cromossomo , Canais de Translocação SEC , Homologia de Sequência de AminoácidosRESUMO
cDNA expression arrays were used to identify mRNA expression markers for cardiac myxoma. The RNA profile analysis suggests that cardiac myxoma should be considered as a stand-alone tissue rather than a pathological modification of particular normal tissue. The analysis reveals a set of genes which are highly and steadily expressed in cardiac myxomas and can serve as an mRNA expression markers of the tumour. Marker status of selected genes was confirmed by reverse transcriptase polymerase chain reaction analysis. Genes MIA (melanoma inhibitory activity) and PLA2G2A (phospholipase A2, group IIA) show the highest specificity as cardiac myxoma markers, since they have more than 10-fold higher RNA level in cardiac myxomas than in any one of 15 normal tissues tested. Among markers of myxoma at least three are participants of phospholipid metabolism: ANXA3, PLA2G2A, and phospholipid transfer protein. Tissue inhibitor of metalloproteinase 1 and secretory leucocyte protease inhibitor are inhibitors of proteases degrading extracellular matrix proteins and participating in cell proliferation regulation. MIA, SPP1, fibromodulin are modulators or participants of the interaction between extracellular matrix proteins and their cell surface receptors. SOX9 is a transcription factor required for chondrocyte differentiation. Calretenin (CALB2) is an intracellular calcium-binding protein with poorly understood function.