[A case of left pulmonary artery sling combined with congenital tracheal stenosis in an adult].

Pulmonary artery sling in adults is a rare congenital vascular malformation usually accompanied by tracheal and bronchial stenosis. Due to its high mortality risk and relatively poor prognosis, it has rarely been reported in adults. We reported a middle-aged patient who presented with shortness of breath, predominantly after activity, since childhood. He was diagnosed with "tracheal stenosis" in another hospital and received symptomatic treatment. The diagnosis of left pulmonary artery sling with congenital tracheal stenosis was confirmed by multi-slice spiral CT (MSCT), airway examination with flexible bronchoscope and 3D image post-processing system. Data from this case and the related literatures have been summarized and analyzed. This will help clinicians to improve their level of diagnosis and treatment.

miR-122-5p inhibits tumor cell proliferation and induces apoptosis by targeting MYC in gastric cancer cells.

MicroRNAs (miRNAs) play crucial roles in the development and progression of human cancers, including Gastric cancer (GC). In this study, we investigated the correlation of miR-122-5p expression with cell proliferation, and apoptosis in a GC cell line. GC cells SCG 7901 were transfected with control, miR-122-5p or miR-122-5p inhibitor and MTT assay, western blot, and BrdU staining were respectively used to investigate the effect of miR-122-5p on GC cell cycle. The overexpression of miR-122-5p could reduce cell proliferation in SCG7901 cells, and BrdU staining finally verified miR-122-5p induced cell growth arrest by upregulation p27 expression in SCG7901cells. On the other hand, cells apoptosis research showed that miR-122-5p induced apoptosis by targeting MYC in SCG7901 cells. Finally, in this study, miR-122-5p was confirmed inhibiting tumor GC cells proliferation and inducing cells apoptosis by targeting MYC. All these findings suggest that miR-122-5p may be involved in progression of GC and could be a new therapeutic target for this disease.

Identification of the CAD gene from Eucalyptus urophylla GLU4 and its functional analysis in transgenic tobacco.

Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final step in lignin biosynthesis. The genus Eucalyptus belongs to the family Myrtaceae, which is the main cultivated species in China. Eucalyptus urophylla GLU4 (GLU4) is widely grown in Guangxi. It is preferred for pulping because of its excellent cellulose content and fiber length. Based on GLU4 and CAD gene expression, a Eucalyptus variety low in lignin content should be obtained using transgenic technology, which could reduce the cost of pulp and improve the pulping rate, and have favorable prospects for application. However, the role and function of CAD in GLU4 is still unclear. In the present study, EuCAD was cloned from GLU4 and identified using bioinformatic tools. Subsequently, in order to evaluate its impact on lignin synthesis, a full-length EuCAD RNAi vector was constructed, and transgenic tobacco was obtained via Agrobacterium-mediated transformation. A significant decrease in CAD expression and lignin content in transgenic tobacco demonstrated a key role for EuCAD in lignin biosynthesis and established a regulatory role for RNAi. In our study, the direct molecular basis of EuCAD expression was determined, and the potential regulatory effects of this RNAi vector on lignin biosynthesis in E. urophylla GLU4 were demonstrated. Our results provide a theoretical basis for the study of lignin biosynthesis in Eucalyptus.

Involvement of ligninolytic enzymes in degradation of wheat straw by Trametes trogii.

AIMS: Wheat straw is generated in billions of tons around the world every year and has not been fully used. This study sought to evaluate the delignification capacity and enzyme production of Trametes trogii MT strain and to clarify the changes of structure and chemical composition of wheat straw during the decay process. METHODS AND RESULTS: The results obtained revealed that the T. trogii MT strain has the ability to degrade lignin, cellulose as well as hemicellulose of wheat straw simultaneously. The strain can produce high activities of laccase, manganese peroxidase, xylanase, carboxymethylcellulase and feruloyl esterase but no lignin peroxidases during the decay process of a 60-day incubation period on wheat straw. Scanning electron microscopy observation and infrared spectroscopy analysis showed the lignin and carbohydrate of wheat straw were degraded with no obvious different levels. The low molecular mass fractions collected from the culture of the MT strains grown in wheat straw powder liquid medium showed high Fe(3+) chelating, reducing capacity and hydroxyl radical and hydrogen peroxide generation. CONCLUSIONS: Trametes trogii MT has a complex mechanism to degrade lignocellulose, in addition to the extracellular enzymatic systems, and has great potential as an attractive micro-organism used for the biological degradation of waste straws. SIGNIFICANCE AND IMPACT OF THE STUDY: This study revealed the dynamic changes of the ligninolytic enzymes of T. trogii MT during the degradation of wheat straw, and suggested that the decay patterns of wheat straw by T. trogii MT had some simultaneous type characteristics.

The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.

Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5' untranslated regions (UTR) of 224 bp, a 3' UTR of 1287 bp with a poly (A) tail and an open reading frame (ORF) of 1542 bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86 kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif ((60)FDRERIPERVVHAKGAL(76)) and a proximal heme-ligand signature motif ((350)RLFSYNDTH(358)) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48 h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs.

Complete sequence of the mitochondrial genome of the Japanese buff-tip moth, Phalera flavescens (Lepidoptera: Notodontidae).

We sequenced the complete mitochondrial genome of Phalera flavescens. The mitogenome is 15,659 bp in length, including 13 protein-coding genes (atp6, atp8, cox1-3, nad1-6, nad4L, cob), two ribosomal RNAs (rrnS and rrnL), 22 transfer RNAs and an AT-rich region, a putative control region (D-loop). Gene order and orientation were found to be identical to those of other completely sequenced lepidopteran mitogenomes. All 13 protein-coding genes start with the common codon ATN, except for the cox1 gene, which uses CGA as the initial codon. Nine of the 13 protein-coding genes stop with codon TAA, while the cox1, cox2, nad5, and nad4 genes stop with the single nucleotide T. All tRNA genes can be folded into canonical cloverleaf secondary structure, except for trnS1, which loses the ''DHU'' arm. Six overlapping sequences totaling 20 bp (1-8 bp for each sequence) and 16 intergenic spacer sequences, totaling 276 bp (1-58 bp for each sequence) are scattered throughout the genome; the largest intergenic spacer is located between the trnQ and nad2 genes. A microsatellite-like structure (AT)(6)ACC(AT)(6) and 16-bp poly-T elements preceded by the ATTTA motif are present in the D-loop region. Additionally, unexpectedly, an extra 190-bp insertion, with unknown function, was found in the small subunit rRNA gene (rrnS); this gene is the longest known (1020 bp) among all of the Lepidoptera.

First Report of Cylindrocladium Black Rot of Peanut Caused by Cylindrocladium parasiticum (Teleomorph Calonectria ilicicola) in Jiangxi Province, China.

In July 2010, a serious disease of peanut (Arachis hypogaea) resembling Cylindrocladium black rot (CBR) was found in Longnan County, Jiangxi Province, China. Symptoms included chlorotic, yellowish and blighted leaves, and wilting of the plants. Taproots and hypocotyls were blackened and rotted. Clusters of reddish orange spherical fruiting bodies appeared in the lesions present on basal stems, pegs, pods, and roots of peanut. Disease incidence reached as much as 50% in some patches of the field. Plants with symptoms were sampled from fields. Microscopic examination revealed that the reddish orange, spherical fruiting bodies were the perithecia and measured 461.6 (337.5 to 609.4) × 395.5 (309.4 to 496.9) µm. With gentle pressure, asci and ascospores were exuded from perithecia. The asci were hyaline, thin walled, and long stalked. Ascospores were hyaline, falcate with one septum, and measured 43.5 (27.3 to 54.5) × 5.6 (4.1 to 6.8) µm with a length/width (L/W) ratio of 7.8 ± 1.3. A fungus with white-to-pale buff border mycelia and yellowish brown pigment was consistently isolated from the edge of basal stem lesions on potato dextrose agar at 25°C. Mycelia grew at temperatures ranging from 8 to 32°C and the optimum was 25 to 26°C. To determine the species, single-conidial isolates of the fungus were cultured on carnation leaf agar for 7 days at 25°C and 12 h of light/dark conditions. Conidia were hyaline, cylindrical with one to three septa (mostly three septa), and measured 49.3 (27.3 to 70.9) × 5.9 (4.1 to 6.8) µm with L/W ratio of 8.4 ± 1.6. Vesicles were globose and measured 5.5 to 10.9 µm in diameter. The fungus was identified as Cylindrocladium parasiticum (teleomorph Calonectria ilicicola) (1,2). A PCR assay was conducted on one representative isolate (JXLN32) by analyzing multilocus sequences of the TUB2 (coding ß-tubulin protein), ACT (coding actin), and CaM gene (coding calmodulin protein) and were amplified and sequenced using the primers reported by Crous et al. (3). Sequences of the studied DNA regions were submitted to GenBank (Accession Nos. TUB2: JF429649; ACT: JQ070809; and CaM: JQ070808). BLAST searches with the existing sequences in GenBank showed that there was 99 to 100% identity with the existing sequences of C. ilicicola (GenBank Accession Nos. TUB2: AY725643; ACT: GQ280446; and CaM: GQ267402). To complete Koch's postulates, inoculum was prepared by mixing the microsclerotia (MS) suspension of the isolate (JXLN32) with soil at a proportion of 10 MS per g of soil. Ten replicate plastic pots containing five peanut seeds (cv. Yueyou 7) each were planted and placed in a glasshouse at 25 ± 2°C. The same number of peanut seeds was used as an uninoculated control. Typical basal stem and roots rot symptoms of CBR were observed in 2 months and C. parasiticum was reisolated from these inoculated diseased plants. No symptoms were detected on the control plants. To our knowledge, this is the first finding of Cylindrocladium black rot in Jiangxi Province, which is the main peanut-producing area in China. The disease has been previously reported in Guangdong Province in southern China but is not known elsewhere (4). Because of its ability to spread through seed and soil and its destructive potential, this pathogen may pose a serious threat to peanut production in China. References: (1) D. K. Bell and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) P. W. Crous et al. Stud. Mycol. 50:415, 2004. (4) R. Pan et al. Plant Pathol. 58:1176, 2009.

First Report of Nectria haematococca Causing Stem Rot of Soybean in China.

In October 2010, soybean (Glycine max) plants growing in commercial soybean fields in Zengcheng City, Guangdong Province developed symptoms consisting of stem and root rot, yellowing, and defoliation of leaves. Reddish, spherical fruiting bodies appeared in lesions that developed on stems. Plants with symptoms were sampled from fields. Fruiting bodies were excised from diseased tissues. Microscopic examination revealed that they were perithecia, globose to pyriform, and measured 197 to 260 µm in diameter and 226 to 358 µm long. When squeezed gently, cylindrical to clavate asci, 7.2 to 9.6 µm in diameter and 75.4 to 92.0 µm long, containing eight ascospores were exuded from the perithecia. Ascospores were ellipsoid to obovate, two celled, slightly constricted at the septum, had longitudinal striations, and measured 4.9 to 6.0 µm in diameter and 10.6 to 15.0 µm long. The fungus was isolated from the basal stem tissues of diseased soybean plants and cultured on potato dextrose agar (PDA) medium amended with streptomycin sulfate. On PDA, the culture developed into blue-pigmented colonies with whitish mycelium that produced oval to cylindrical microconidia. Microconidia had 0 to 1 septum, ranged from 2.5 to 5.2 × 7.6 to 29.4 µm, and were produced on monophialides. Macroconidia were cylindrical to falcate, thick walled, 2 to 5 septa, and 3.5 to 6.0 × 25.4 to 66.8 µm. Chlamydospores were present and ranged from 6.8 to 13.6 × 5.5 to 9.5 µm. Orange-to-reddish perithecia were readily formed in old culture. These morphological characteristics were consistent with descriptions of Nectria haematococca (anamorph Fusarium solani) (1). The rDNA internal transcribed spacer (ITS) region and the fragment of translation elongation factor 1-alpha (EF1-α) genes of the fungus were amplified, respectively, with universal primers ITS1/ITS4 and ef1/ef2 primers and sequenced. BLAST searches showed that the ITS sequences of three isolates (GenBank Accession Nos. JN015069, JN190942, and JN190943) had 99% similarity with those of N. haematococca(GenBank Accession Nos. DQ535186, DQ535185, and DQ535183) and the EF1-α sequences of three isolates (GenBank Accession Nos. JN874641, JN874642, and JN874643) had 100% similarity with those of F. solani (GenBank Accession Nos. DQ247265 and DQ247327). Completion of Koch's postulates confirmed the pathogenicity of the isolates in a replicated experiment. Thirty-day-old soybean seedlings of cultivar Huaxia No. 3 were inoculated by soaking their root systems in a conidial suspension (106 conidia per ml) for 30 min and then transplanted in plastic pots (20 cm in diameter) and incubated at 25 ± 2°C in a greenhouse. Control plants were treated with sterile water in the same way. There were four plants per pot and there were six replicates for each treatment. Within 3 weeks, more than 70% of the inoculated plants exhibited symptoms of leaf yellowing, stem rot, and root rots while control plants were symptomless. N. haematococca was reisolated from the diseased plants. To our knowledge, this is the first report of N. haematococca causing stem rot of soybean in China and the first description of sexual reproduction of F. solani causing soybean stem rot in nature. This pathogen may pose a serious threat to soybean production in China where soybean is a main crop. Reference: (1) C. Booth. The Genus Fusarium. CAB International, Wallingford, UK, 1971.

First Report of Peanut Foot Rot Caused by Neocosmospora vasinfecta var. africana in Jiangxi Province, China.

From June 2009 to November 2010, a disease was observed on peanut (Arachis hypogaea L.) in Ganzhou City, Jiangxi Province, China. Infected plants initially exhibited yellow leaves, then defoliated, and finally wilted and died. Basal stems, pegs, pods, and roots became black and rotted, with many orange-brown spherical fruiting bodies emerging on the lesions. Disease incidence reached as high as 30% in some plots, especially in those covered with plastic sheets after planting to control weeds. Isolations from 68 diseased plants were conducted on potato dextrose agar (PDA) amended with streptomycin sulfate and incubated at 25°C. A fungus was consistently isolated from the edge of the lesions. Mycelia grew at a linear rate of 3.9 mm per day on PDA at 25°C forming a pale buff, floccose colony with abundant orange-red perithecia. Perithecia were globose to pyriform, ostiolate and with a short neck, and measured 151 to 353 × 141 to 313 µm. Asci were narrowly cylindrical to clavate, thin walled with a short stalk, measured 100 to 160 µm high and 9 to 15 µm in diameter, and were eight spored. Ascospores were uniseriately arranged, globose to ellipsoid, nonseptate, thick walled, hyaline to pale yellow, and measured 7 to 16 × 7 to 12 µm. Scanning electron microscopy revealed that the wall of the ascospores had cerebriform ornamentation. Conidia were cylindrical to oblong-ellipsoidal, hyaline, most one celled, and measured 3 to 15 × 1 to 5 µm, aggregating in a gelatinous mass on the tip of the conidiogenous cell, which usually arose directly from the vegetative mycelium. The fungus was identified as Neocosmospora vasinfecta var. africana (anamorph Acremonium sp.) (1). The rDNA internal transcribed spacer (ITS) region of the fungus was amplified with universal primers ITS1/ITS4 and sequenced. The sequences of a representative isolate (No. N-JXLN02) were submitted to GenBank (Accession No. JF708085) and BLAST searches showed 99 to 100% similarity with sequences of N. vasinfecta deposited in GenBank (Accession Nos. HM461900, HM461901, and AY381142 ). Pathogenicity tests were conducted on 36 2-week-old peanut seedlings, cv. Yueyou No. 13, in plastic pots. Plants were inoculated by drenching the soil near the shoot with a mixed suspension of conidia and ascospores (105 spores per ml). Control plants were treated with sterile water. All plants were then incubated in a moist chamber at 25 ± 2°C. Fifteen days after inoculation, all inoculated plants showed lesions on basal stems and black root rot similar to that observed on naturally infected plants. No disease was observed on control plants. The pathogen was reisolated from infected tissues. To our knowledge, this is the first finding of Neocosmospora foot rot of peanut in Jiangxi Province, where outbreaks of this disease have been observed in several counties. The pathogen will pose a threat to peanut, which is a major oil crop in China. It has been previously reported in Taiwan (2) and recently in Guangdong Province, though the subspecies of the pathogen was not identified in the latter case (3). References: (1) P. F. Cannon and D. L. Hawksworth. Trans. Br. Mycol. Soc. 82:673, 1984. (2) J. W. Huang et al. Plant Pathol. Bull. 1:203, 1992. (3) R. Pan et al. Plant Pathol. 59:1172, 2010.

First Report of Soybean Neocosmospora Stem Rot Caused by Neocosmospora vasinfecta var. vasinfecta in China.

During October 2009, the occurrence of a disease on soybean (Glycine max) was observed in several fields in Boluo County and Zengcheng City, Guangdong Province. Top leaves of infected plants initially turned yellow and plants eventually were defoliated, while stems and roots became black and rotted. The stem lesions sometimes extended 10 to 15 cm upward from the soil surface. Orange-to-brown spherical fruiting bodies, which were very similar with those of the soybean red crown rot pathogen, scattered or congregated on the stem lesions. Plants with symptoms were sampled from fields. Fruiting bodies were excised from diseased tissues. Microscopic examination revealed that they were perithecia, globose to pyriform, ostiolate with a short neck, and measured 160 to 298 × 151 to 235 µm. Under gentle pressure, asci and ascospores were exuded from these perithecia. Asci were eight spored, narrowly cylindrical to clavate, thin walled, with a short stalk, and measured 58 to 124 µm long and 8 to 15 µm in diameter. Ascospores were uniseriately arranged, globose to ellipsoid, thick walled, one celled, hyaline to pale, and measured 14 to 17 × 8 to 12 µm. Isolation was made from stem tissues at the edge of disease lesions on potato dextrose agar (PDA) amended with streptomycin sulfate and incubated at 25°C. Mycelia were white and floccose. Conidia were cylindrical to oblong-ellipsoidal, hyaline, one celled, and measured 6 to 22 × 2 to 5 µm, aggregating in a slimy mass on the apex of the conidiogenous cell. Abundant orange-to-brown spherical perithecia were produced on the colony. Ascospores had walls with a rugose ornamentation that could be clearly seen under a scanning electron microscope. The fungus was identified as Neocosmospora vasinfecta var. vasinfecta (anamorph Acremonium sp.) (1). The internal transcribed spacer (ITS) region of rDNA of two isolates were amplified with universal primers ITS1/ITS4 and sequenced (GenBank Accession No. JF705861 and JF705862), and comparisons with GenBank accessions showed 99% similarity with N. vasinfecta strain Pec070 (Accession No. FJ940902) and strain NRRL22497 (Accession No. AY381142). Pathogenicity tests were conducted. Five, 3-week-old seedlings of soybean cv. Huaxia No. 3 planted in plastic pots (20 cm in diameter) were wounded with a needle at the base of the stem below the soil line and near the root system, and then inoculated by drenching the soil with a conidial suspension (105 per ml). Control plants were inoculated with sterile water. There were six replicates for each treatment. The treated plants were incubated at 25 ± 2°C in a greenhouse. All inoculated plants exhibited symptoms of leaf yellowing and black rot of stems and roots 3 weeks after inoculation. N. vasinfecta var. vasinfecta was reisolated from the diseased plants. All control plants remained healthy. To our knowledge, this is the first observation of Neocosmospora stem rot of soybean in China. The pathogen could pose a threat to soybean, which is a major crop in China. This disease has been previously reported in the United States though the anamorph of the pathogen has either not been identified or has been identified as a Cylindrocarpon sp. (2,4). This fungus is also associated with human infections (3). References: (1) P. F. Cannon and D. L. Hawksworth. Trans. Br. Mycol. Soc. 82:673, 1984. (2) F. A. Gray et al. Plant Dis. 64:321, 1980. (3) P. Manikandan et al. Med. Mycol. 46:279, 2008. (4) D. V. Phillips. Phytopathology 62:612, 1972.

In vitro dopaminergic neuroprotective and in vivo antiparkinsonian-like effects of Delta 3,2-hydroxybakuchiol isolated from Psoralea corylifolia (L.).

Cocktail recipes containing Psoralea corylifolia seeds (PCS) are used to empirically treat Parkinson disease. A PCS isolate Delta(3),2-hydroxybakuchiol (BU) can inhibit dopamine uptake in dopamine transporter (DAT) transfected Chinese hamster ovary (CHO) cells, and dopamine reuptake blockade may provide an alternative approach for ameliorating parkinsonism. Here, we assessed the potential dopaminergic neuroprotective, and antiparkinsonian-like activity of BU. BU sample size was increased by using a scale-up extraction paradigm. Pharmacologically, BU significantly protected SK-N-SH cells from 1-methyl-4-phenylpyridinium (MPP(+)) insult, produced striking inhibitory actions on dopamine/norepinephrine uptake and WIN35,428 binding in synaptosomes on in vivo administration, and significantly preventing poor performance on rotarod and dopaminergic loss in substantia nigra in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice. BU acts by protecting dopaminergic neurons from MPP(+) injury and preventing against MPTP-induced behavioral and histological lesions in the Parkinson's disease (PD) model, possibly by inhibiting monoamine transporters. These findings suggest that BU could be meaningful in PD treatment.

Astaxanthin protecting myocardial cells from hypoxia/reoxygenation injury by regulating miR-138/HIF-1α axis.

OBJECTIVE: To investigate astaxanthin (AST) protecting myocardial cells from hypoxia/reoxygenation (H/R) injury by regulating miR-138/HIF-1α axis. MATERIALS AND METHODS: Myocardial cells were collected and divided into a control group, a H/R group, and a H/R+AST group. The H/R injury model was established, and cells in the H/R+AST group were given AST before modeling. The cell survival rate, contents of myocardial enzymes, and apoptosis were detected. RESULTS: The survival rate in the H/R group reduced and was lower than that in the H/R+AST group (p<0.05). Compared with the control group, activities of myocardial enzymes significantly increased in the H/R group but those were inhibited in the H/R+AST group (p<0.05). The apoptotic rate in the H/R group significantly increased compared with the control group but that significantly decreased compared with the H/R+AST group (p<0.05). The expression of cleaved caspase-9 and caspase-3 increased in the H/R group (p<0.05), and was higher than that in the H/R+AST group (p<0.05). The expression levels of miR-138 and HIF-1α were detected. MiR-138 level significantly decreased in the H/R group but increased in the H/R+AST group (p<0.05). Compared with the control group, HIF-1α content significantly increased in the H/R group but that was significantly inhibited in the H/R+AST group (p<0.05). The Luciferase reporter gene assay confirmed that HIF-1α was the target gene of miR-138. After miR-138 mimics and HIF-1α siRNA were transfected into myocardial cells, the cell survival rate significantly increased, and activities of myocardial enzymes were significantly inhibited in the H/R+AST+miR-138 mimics and H/R+AST+HIF-1α siRNA groups (p<0.05). The apoptotic rate significantly decreased, and contents of cleaved caspase-9 and caspase-3 were significantly inhibited in the miR-138 mimics and HIF-1α siRNA groups (p<0.05). CONCLUSIONS: AST can exert a protective function in myocardial cells via regulating the expression of miR-138/HIF-1α axis.

Rosuvastatin inhibits inflammatory response and resists fibrosis after myocardial infarction.

OBJECTIVE: To study the effect of rosuvastatin on myocardial infarction in rats and its mechanism of action. MATERIALS AND METHODS: 24 Sprague-Dawley (SD) rats were randomly divided into 3 groups: intensive statin group (n=8), myocardial infarction control group (n=8) and sham-operation group (n=8). The left anterior descending coronary artery was ligated to establish myocardial infarction models. Rats in intensive statin group were treated with gavage via rosuvastatin (1 mg × kg) and 1.5 mL distilled water suspension at 3 d before operation, while rats in the other two groups received gavage via the same amount of distilled water till 4 weeks after operation. Venous blood was collected using capillary glass tubes at 3 d before operation (before medication) and the last day in the 4th week after operation. Interleukin-6 (IL-6) was detected via chemiluminescence assay, and tumor necrosis factor-α (TNF-α) was detected via immunofluorescence assay. Hematoxylin and eosin (HE) staining and Masson staining were performed for myocardium to detect the inflammation and fibrosis. Finally, the expressions of inflammatory protein p65, peroxisome proliferator-activated receptor (PPAR) and fibrin were detected via Western blotting, and the Snail expression was detected by immunohistochemical assay. RESULTS: The survival rate and cardiac function of rats in intensive statin group were superior to those in control group. HE staining and detection of blood IL-6 and TNF-α, and p65 and PPAR protein expressions revealed that the inflammatory levels in the body and myocardium of rats in intensive statin group were decreased compared with those in control group. Masson staining and detection of fibrin level showed that the myocardial fibrosis level of rats in intensive statin group was reduced compared with that in control group. CONCLUSIONS: Rosuvastatin can reduce the level of myocardial fibrosis through alleviating the inflammatory response in rats with myocardial infarction.

Effects of rosuvastatin on pentraxin 3 level and platelet aggregation rate in elderly patients with acute myocardial infarction undergoing elective interventional therapy: a double-blind controlled study.

OBJECTIVE: To investigate clinical effects of rosuvastatin on blood lipid levels, hemorheological profiles, vascular endothelial function, pentraxin 3 (PTX-3) level, the number of granule membrane glycoprotein (GMP-140) molecules and platelet aggregation rate in elderly patients with acute myocardial infarction (AMI) undergoing elective percutaneous coronary intervention (PCI). PATIENTS AND METHODS: Total of 120 elderly patients admitted with AMI undergoing elective PCI from July 2014 to January 2016 were selected. The patients were divided into the control group and the experimental group based on the rule of random number generation and double-blind controlled trial, 60 cases in each group. All of 120 patients were treated with routine medications; the experimental group was orally administered with rosuvastatin 1 week before PCI. Blood lipid levels, hemorheological profiles, vascular endothelial function, PTX-3, the number of GMP-140 molecules and platelet aggregation rate were compared between two groups before treatment with rosuvastatin and 10d after elective PCI. RESULTS: Triglycerides, plasma total cholesterol, and low-density lipoprotein levels were significantly lower (p<0.05) in the experimental group when compared with the control group; plasma viscosity, fibrinogen, the viscosity of blood in the high shear rates and in the low shear rates in the experimental group were significantly lower than those of the control group (p<0.05); FMD and NMD in the experimental group were significantly higher than those of the control group (p<0.05); ET-1, TXA2 levels in the experimental group were lower, however, PGI2, NO as well as NOS in the experimental group were higher, when compared the control group, the differences were statistically significant (p<0.05); PTX-3, the number of GMP-140 molecules and platelet aggregation rate in the experimental group were significantly lower than those of the control group (p<0.05). CONCLUSIONS: Oral administration of rosuvastatin 1 week before PCI can significantly improve the blood lipid levels and hemorheological profiles, enhance endothelial function, reduce the PTX-3 level and the number of GMP-140 molecules, decrease the platelet aggregation rate, therefore improving prognosis in elderly patients with AMI undergoing PCI.

A general approach to cyathin diterpenes. Total synthesis of allocyathin B(3).

[reaction: see text] The synthesis of allocyathin B(3) from an advanced intermediate possessing the ring system and relative stereochemistry but lacking the isopropyl and hydroxymethyl groups is reported. The isopropyl group was introduced by radical cyclization of a methyl propargyl acetal of an alpha-bromo ketone, and the hydroxymethyl group was generated by Pd-catalyzed carbonylation of a vinyl triflate. The route provides functionalized intermediates that could allow access to more complex members of the cyathin family of diterpenes.

Diversity of chloroplast DNA SSRs in wild and cultivated soybeans: evidence for multiple origins of cultivated soybean.

Soybean [ Glycine max (L.) Merr.] is one of the major crops in the world and was domesticated from a wild progenitor, Glycine soja Sieb. & Zucc., in East Asia. In order to address the questions concerning the evolution and maternal lineage of soybean, we surveyed the variation in chloroplast DNA simple sequence repeats (cpSSR) of 326 wild and cultivated soybean accessions that were collected from various Asian countries. Twenty-three variants were detected at six cpSSRs in the accessions tested. All of the variants were found in wild soybean, whereas only 14 variants existed in the cultigen. Combining the variants at the six cpSSRs gave 52 haplotypes in the former and eight haplotypes in the latter. Both analyses indicated a considerably higher genetic diversity in the wild soybean. Around 75% of the cultivated accessions tested possessed a common haplotype (no. 49), which was detected in only seven wild accessions, six from southern Japan and one from southern China. The predominant haplotype in the cultigen may therefore have originated from a rare haplotype of the wild soybean that is presently distributed in the southern areas of Japan and China. The remaining seven haplotypes in the cultigen were distributed regionally, and except for three rare haplotypes, largely overlapped with the distributions of wild accessions with the same respective haplotypes. Our results strongly suggest that the cultivated soybeans with different cpDNA haplotypes originated independently in different regions from different wild gene pools and/or hybrid swarms between cultivated and wild forms.

Transformation of the host-selective toxin destruxin B by wild crucifers: probing a detoxification pathway.

The destruxin B detoxification pathway present in Sinapis alba is also present in three unrelated species, Camelina sativa, Capsella bursa-pastoris, and Eruca sativa, suggesting a conservation of this pathway across crucifers. The chemical structure of a destruxin B metabolite, (6'-O-malonyl)hydroxydestruxin B beta-D-glucopyranoside, was also establised. Considering that Camelina sativa and Capsella bursa-pastoris detoxify destruxin B and produce the phytoalexins camalexins, these wild crucifers appear to represent unique and perhaps useful sources of blackleg resistance in strategic plant breeding.

[Comparison of susceptibility testing results of M. tuberculosis by different methods].

OBJECTIVE: To compare the susceptibility testing results of M. tuberculosis detected with different standards and methods. METHOD: 158 strains of M. tuberculosis isolated from clinical patients were detected for the drug sensitivity by Chinese standard and method (absolute concentration method), 1% resistance ratio and absolute concentration method of WHO/IUATLD standard. RESULTS: The drug-resistant rate detected by the standard of WHO/IUATLD was 53.8%(85/158), while by the Chinese standard 50.0%(79/158). The drug-resistant rates to INH, SM, EMB and RFP by WHO/IUATLD standard were 41.8%(66/158), 34.2%(54/158), 22.1%(35/158) and 27.8%(44/158) respectively, whereas by the Chinese standard 34.2%(54/158), 30.4% (48/158), 18.4%(29/158) and 27.2%(43/158) respectively. There was no significant difference between the results detected with WHO/IUATLD and Chinese standard, except for the results of INH. There was no significant difference between the results detected with WHO/IUATLD standard by 1% resistant ratio and absolute concentration methods. CONCLUSION: The results of drug-sensitivity were interfered by the detected standards but not by the tested methods.

[Observation on acupoint electric potential in pilots under vestibular stimulation].

To study the possibility of using acupoint electric potential as an index for evaluation of pilot's reaction to vestibular stimulation, electric potential was measured on both sides of left and right Neiguan points and Zusanli points in 80 pilots. Rotation chair was used as the method of stimulation. The results showed that changes of acupoint electric potentials were not significant (P >0.05) during 5 min uniform rotation. While the changes under head motion stimulation were significant (P<0.05 or 0.01) as compared with the pretest values. Relationship between symptoms and the corresponding acupoint potential changes remain to be studied further.