Drug Reprofiling to Identify Potential HIV-1 Protease Inhibitors.

The use of protease inhibitors in human immunodeficiency virus type 1 (HIV-1) treatment is limited by adverse effects, including metabolic complications. To address these challenges, efforts are underway in the pursuit of more potent and less toxic HIV-1 protease inhibitors. Repurposing existing drugs offers a promising avenue to expedite the drug discovery process, saving both time and costs compared to conventional de novo drug development. This study screened FDA-approved and investigational drugs in the DrugBank database for their potential as HIV-1 protease inhibitors. Molecular docking studies and cell-based assays, including anti-HIV-1 in vitro assays and XTT cell viability tests, were conducted to evaluate their efficacy. The study findings revealed that CBR003PS, an antibiotic currently in clinical use, and CBR013PS, an investigational drug for treating endometriosis and uterine fibroids, exhibited significant binding affinity to the HIV-1 protease with high stability. Their EC50 values, measured at 100% cell viability, were 9.4 nM and 36.6 nM, respectively. Furthermore, cell-based assays demonstrated that these two compounds showed promising results, with therapeutic indexes higher than 32. In summary, based on their favorable therapeutic indexes, CBR003PS and CBR013PS show potential for repurposing as HIV-1 protease inhibitors.

Identificación de los niveles de ansiedad y depresión en pacientes con insuficiencia renal crónica de una unidad de hemodiálisis de la ciudad de Armenia - Quindío / Identification of anxiety and depression levels in patients with chronic kidney failure from a hemodialysis unit in the city of Armenia - Quindío

El presente trabajo tuvo como fin identificar los niveles de ansiedad y depresión en pacientes con insuficiencia renal crónica (IRC), de una unidad de hemodiálisis de la ciudad de Armenia. Para ello se realiza una investigación de carácter descriptivo-cuantitativa en una muestra de 12 pacientes con IRC a los que se les aplicó la escala hospitalaria de ansiedad y depresión de Hamilton. Se encontró niveles significativos de ansiedad en el 66% de los encuestados, y depresión significativa en el 28%. Estas altas cifras indican la necesidad de realizar labores de acompañamiento psicológico durante el proceso médico en pro del mejoramiento de las condiciones de vida de las personas con IRC o cualquier otra patología de cronicidad que cause alteraciones en los aspectos psicosociales.

The present Project had as purpose to identify the levels of anxiety and depression in patients with chronic renal failure (CRF) of a hemodialysis unit of the city of Armenia. For that end, a descriptive, quantitative investigation was made in a sample of 12 patients with CRF, who were administered the Hamilton hospital anxiety and depression scale. There were found significant levels of anxiety in 66% of the sample, and significant levels of depression in the 28%. These high figures indicate the necessity to make psychological support work in the medical process to the improvement of life conditions of the people with CRF or any other chronic pathology that causes changes in the psychosocial aspects.