RESUMO
A new variant of the normal minor component Hb A(2) has been detected in a family that lives in Sphakiá, Crete. Chemical studies of this abnormal hemoglobin, designated Hb A(2) delta Sphakiá, indicates a substitution of the histidyl residue number two of the delta-chain by an arginyl residue.
Assuntos
Hemoglobinas Anormais , Adulto , Criança , Cromatografia , Eletroforese , Grécia , Humanos , Técnicas In VitroRESUMO
Small unilamellar liposomes were optimised for cell phenotyping by indirect immunofluorescence. This involved selection and covalent attachment to the liposome of a polyspecific ligand for cell-bound antibody. For this purpose sheep anti-mouse antibody was preferred to protein A because of its ability to attach to cell-bound IgG1 as well as IgG2 at physiological pH. The maximally fluorescent concentration of encapsulated carboxyfluorescein was determined to be 20 mM and liposomes thus comprised gave up to a nine-fold increase in mean cell fluorescence when compared with sheep anti-mouse antibody conjugated to fluorescein isothiocyanate. There was no parallel increase in background fluorescence. Liposomes retained their targeting and fluorescence properties after 3 months storage. They could be sterilised and were as versatile in use as FITC-antibody conjugates.
Assuntos
Imunofluorescência , Lipossomos , Animais , Citometria de Fluxo , Fluoresceína-5-Isotiocianato , Fluoresceínas/administração & dosagem , Humanos , Camundongos , Fenótipo , Ovinos , TiocianatosRESUMO
The oxyhaemoglobin dissociation curve (ODC) was investigated in 15 patients with Eales's disease and 11 controls to test the hypothesis that Eales's disease is caused by retinal hypoxia secondary to impaired oxygen release from the blood. Patients with the peripheral form of Eales's disease did not have any significant difference in the P50 values when compared with the controls. However, the P50 values were significantly lower (P < 0.005) for the patients with mixed retinopathy. This degree of increased oxygen affinity is not physiologically significant but may reflect an intrinsic abnormality of the red cells in these cases.
Assuntos
Oxigênio/sangue , Hemorragia Retiniana/sangue , Adulto , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Neovascularização Patológica , Oxiemoglobinas/metabolismoRESUMO
In a group of young patients with thalassaemia and iron overload treated by subcutaneous infusions of desferrioxamine we have found a number of minor alterations in retinal function. The incidence of such changes is not related to drug dosage or to ferritin level but to abnormality of the extended glucose tolerance test.