[PHYTOCORRECTION OF IMMUNOLOGICAL AND BIOCHEMICAL CHANGES IN THE COMBINED IMPACT OF COAL DUST AND HIGH DOSE OF RADIATION].

The objective of this researsh is to study the effects of Eminium Regelii phytopreparation (ERP) on immune status and free radical oxidation in the tissues of the adrenal glands and immunocompetent organs after combined exposure to 6 Gy dose of gamma irradiation and coal dust (remote period). The study was realized on 30 white laboratory male rats of the Wistar line, weighing 240±20g, that were divided into equal 3 groups: I group - intact, ІІ group - were exposured to combined effects of coal dust and gamma irradiation, III group - were exposured to combined effects and in parallel taking phytopreparation Eminium Regel. The animals of II and III groups were irradiated 90 days prior to the study at the TERAGAM 60Co radiotherapy unit ("ISOTREND spol. S.r.o.", Czech Republic) in dose of 6 Gy once. Experimental animals received phytopreparation of ER 2.5 mg/kg per day on calculate of body mass for 14 days. The results of the conducted studies showed that in the long-term period after the actions of the sublethal dose of gamma radiation and coal dust, significant changes were revealed that were characterized by a decrease in immunological reactivity, increased lipoperoxidation and inhibition of antioxidant defense activity of the organism. After exposure to ER, oxidative stress was alleviated, sufficient restoration of antioxidant protection and immune system indices, which were disrupted by the combined effects of a single high dose of radiation and a prolonged three-month inhalation of coal dust.

[METABOLIC PROCESSES OF ORGANISM IN REMOTE PERIOD AFTER THE COMBINED EFFECTS OF RADIATION AND EMOTIONAL STRESS].

Aim of the research was to study the role of free radical oxidation in the tissues of adrenal and immune organs and cells in remote period after combined effects of sublethal dose of gamma radiation (6 Gy) and emotional stress. Forty male Wistar rats were divided into four groups: I - control; II - exposed to emotional stress; III - exposed to radiation; IV - under the combined effects of emotional stress and radiation. Emotional stress in groups II and IV was simulated by tail suspension. In groups III and IV rats were irradiated once 90 days before the investigation at 6 Gy via TERAGAM Сo60 («ISOTREND spol. s.r.o.¼, Czech Republic). The results of study showed that after the long term exposure gamma radiation has inhibitory effect of the radiation factor in the antioxidant protection. Ionizing radiation combined with emotional stress in remote period has a more pronounced effect on the formation of lipid hyper peroxidation syndrome than separately. Influence of the combined effects of emotional stress and ionizing radiation resulted in increased levels of DC and MDA, inhibition of enzyme activity of catalase and glutathione reductase in almost all the study objects, resulting in the development of dual-oxidative stress.

Free radical oxidation in rats in the delayed period after combined exposure to dust and radiation.

We studied the intensity of free radical processes in organs (liver, spleen, thymus, lymph nodes of the small intestine, and adrenal glands) and cells (lymphocytes) in the delayed period after combined exposure to a sublethal dose of γ-radiation (6 Gy) and asbestos dust particles. Combined exposure was followed by accumulation of LPO metabolites (diene conjugates and malonic dialdehyde) in homogenates of the studied organs and resulted in antioxidant defense failure in experimental animals. Ionizing radiation have a pivotal role in these processes.